##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936901_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 178362 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912144963613326 31.0 31.0 34.0 30.0 34.0 2 31.987160942353192 31.0 31.0 34.0 30.0 34.0 3 32.040008521994594 33.0 31.0 34.0 30.0 34.0 4 35.755749543064105 37.0 35.0 37.0 35.0 37.0 5 35.68250524214799 37.0 35.0 37.0 33.0 37.0 6 35.83312028346845 37.0 35.0 37.0 35.0 37.0 7 35.777772171202386 37.0 35.0 37.0 35.0 37.0 8 35.773684977741894 37.0 35.0 37.0 33.0 37.0 9 37.326723180946615 39.0 37.0 39.0 34.0 39.0 10-11 37.23325876588063 39.0 37.0 39.0 33.5 39.0 12-13 37.17632959935412 39.0 37.0 39.0 33.0 39.0 14-15 38.010781444478084 40.0 37.0 41.0 33.0 41.0 16-17 37.99173310458506 39.0 37.0 41.0 33.0 41.0 18-19 38.28892364965631 40.0 37.5 41.0 34.0 41.0 20-21 38.371359930926985 40.0 38.0 41.0 34.0 41.0 22-23 38.25694094033483 40.0 37.5 41.0 34.0 41.0 24-25 38.032661104943884 40.0 37.0 41.0 33.0 41.0 26-27 37.714381426537045 39.5 36.5 41.0 33.0 41.0 28-29 37.565044684405876 39.0 36.0 41.0 33.0 41.0 30-31 37.41518653076328 39.0 36.0 41.0 33.0 41.0 32-33 36.95092003902177 39.0 35.0 41.0 32.0 41.0 34-35 36.576389589710814 38.5 35.0 41.0 31.0 41.0 36-37 36.19723651898947 38.0 35.0 40.5 30.5 41.0 38-39 35.865299222928655 38.0 35.0 40.0 30.0 41.0 40-41 35.64295365604781 38.0 35.0 40.0 30.0 41.0 42-43 35.087759724605014 38.0 35.0 40.0 28.0 41.0 44-45 34.43025420212825 38.0 34.5 40.0 23.5 41.0 46-47 33.5437509110685 38.0 33.5 40.0 14.0 41.0 48-49 32.68002993911259 38.0 33.0 40.0 2.0 41.0 50-51 32.16344008252879 37.0 33.0 40.0 2.0 41.0 52-53 31.911570289635684 37.0 32.0 40.0 2.0 41.0 54-55 31.624283759993723 37.0 31.5 40.0 2.0 41.0 56-57 31.19626658144672 36.0 31.0 39.5 2.0 41.0 58-59 30.84317567643332 36.0 31.0 39.0 2.0 40.5 60-61 30.320191520615378 35.0 29.0 39.0 2.0 40.0 62-63 30.03385810878999 35.0 29.0 38.5 2.0 40.0 64-65 29.6621617833395 35.0 29.0 38.0 2.0 40.0 66-67 29.11002063219744 35.0 28.0 37.0 2.0 39.0 68-69 28.741601350063355 34.0 27.5 37.0 2.0 39.0 70-71 28.165517318711384 34.0 26.5 36.0 2.0 38.5 72-73 27.68128581200031 34.0 26.0 35.5 2.0 37.5 74-75 27.266766463708638 33.0 26.0 35.0 2.0 37.0 76-77 25.966514728473555 31.5 24.5 34.0 2.0 35.5 78-79 26.695843285004653 33.0 25.0 35.0 2.0 36.0 80-81 26.426909879907157 33.0 24.5 35.0 2.0 36.0 82-83 26.08503492896469 33.0 23.5 35.0 2.0 35.0 84-85 25.73402966999697 33.0 22.5 35.0 2.0 35.0 86-87 25.53744351375293 33.0 20.5 35.0 2.0 35.0 88-89 25.07229959296263 32.0 19.0 34.5 2.0 35.0 90-91 24.50386573373252 32.0 14.0 34.0 2.0 35.0 92-93 24.06061548984649 31.0 4.5 34.0 2.0 35.0 94-95 23.399950662136554 31.0 2.0 34.0 2.0 35.0 96-97 22.633360805552755 31.0 2.0 34.0 2.0 35.0 98-99 21.938655655352598 31.0 2.0 34.0 2.0 35.0 100-101 18.81246285643803 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.011402094376894212 1101 2 0.059088599794321794 1101 3 -0.01573973659809269 1101 4 0.07788312702056288 1101 5 0.16504661879108795 1101 6 0.1592405663942671 1101 7 0.09698669814011396 1101 8 0.15972912433507958 1101 9 0.4200222033203289 1101 10-11 0.3246120290561052 1101 12-13 0.2985840335256782 1101 14-15 0.28196978729845057 1101 16-17 0.2314459466862857 1101 18-19 -0.09829731086138338 1101 20-21 -0.07349831510045846 1101 22-23 0.08256761085327469 1101 24-25 0.11015055386172179 1101 26-27 0.22628415498619603 1101 28-29 0.05178961920298519 1101 30-31 0.4860406342899779 1101 32-33 0.8277733326782908 1101 34-35 0.9541559979833281 1101 36-37 1.0970418859274957 1101 38-39 0.8433271573248007 1101 40-41 0.5624705381556652 1101 42-43 1.177871571036043 1101 44-45 1.3489158035073388 1101 46-47 1.708301034892095 1101 48-49 1.834961048628152 1101 50-51 2.124897984848097 1101 52-53 2.257694251521851 1101 54-55 1.4269958964495082 1101 56-57 2.456248327871652 1101 58-59 1.9879053378745937 1101 60-61 2.3103961339072647 1101 62-63 2.0847274663484434 1101 64-65 2.2695210569321453 1101 66-67 1.9903376736568674 1101 68-69 1.789532019819525 1101 70-71 1.9868559319460815 1101 72-73 2.6122537602323312 1101 74-75 2.100027281055457 1101 76-77 1.7586468783487703 1101 78-79 1.8102800825678536 1101 80-81 1.697153226799447 1101 82-83 2.103678101811436 1101 84-85 2.401899589711242 1101 86-87 1.3625624553135367 1101 88-89 1.33558348337802 1101 90-91 1.8868345819423915 1101 92-93 1.9571142515777211 1101 94-95 2.134992864810993 1101 96-97 2.858180507199048 1101 98-99 2.5329037773960543 1101 100-101 1.263587655266388 1102 1 0.2624900856729617 1102 2 0.34019835091631734 1102 3 0.37049717350662803 1102 4 0.036097461305892864 1102 5 0.12336295226042182 1102 6 0.17685242549939773 1102 7 -0.27197835013949856 1102 8 -0.24384764705074957 1102 9 0.038613254591915336 1102 10-11 0.23747072618652965 1102 12-13 0.49173648830603867 1102 14-15 0.26041395974507253 1102 16-17 0.6363657073282027 1102 18-19 0.41768256129964243 1102 20-21 0.46352738869391175 1102 22-23 0.4649625516937448 1102 24-25 0.40358520638449846 1102 26-27 0.4864840733868476 1102 28-29 0.6053221909424096 1102 30-31 0.43582303603076866 1102 32-33 0.9902410329366873 1102 34-35 1.3901855097472335 1102 36-37 1.2801134213552103 1102 38-39 1.5874282045164207 1102 40-41 1.9321495807233262 1102 42-43 1.6328729310251617 1102 44-45 1.2573375361334769 1102 46-47 1.4644209859324846 1102 48-49 1.960930040876221 1102 50-51 2.237573083447309 1102 52-53 2.264630196034293 1102 54-55 2.322140735290798 1102 56-57 2.211552285439989 1102 58-59 2.19154670874363 1102 60-61 2.501083608407466 1102 62-63 2.5913744131728755 1102 64-65 2.38923409922781 1102 66-67 2.4765507839519856 1102 68-69 2.7899910161475567 1102 70-71 2.0054707830272704 1102 72-73 1.754321623689421 1102 74-75 1.9735652927513634 1102 76-77 1.6430308032773695 1102 78-79 1.74336861785957 1102 80-81 1.9670260678167182 1102 82-83 1.8712049615965576 1102 84-85 2.18671531118547 1102 86-87 2.534958076148566 1102 88-89 2.268043023701697 1102 90-91 2.327896053450619 1102 92-93 2.5334776406706077 1102 94-95 2.284880665708588 1102 96-97 2.95759571187741 1102 98-99 3.3459052733840835 1102 100-101 3.071931188518125 1103 1 0.36585837604507887 1103 2 0.20719825290592553 1103 3 0.15003913828822846 1103 4 0.3070308469114309 1103 5 0.13652901452968536 1103 6 0.09733332980858478 1103 7 0.11738425563004284 1103 8 0.25515591399338433 1103 9 0.15491891632539279 1103 10-11 0.13918264098283117 1103 12-13 0.24716789736873324 1103 14-15 0.5071224441777531 1103 16-17 0.610278608762421 1103 18-19 0.3116026501623921 1103 20-21 0.17331862656512698 1103 22-23 0.4444293751770374 1103 24-25 0.030590335594858686 1103 26-27 0.5198076056812724 1103 28-29 -0.1385929974008846 1103 30-31 0.09838960142003117 1103 32-33 0.06814295720732844 1103 34-35 0.09422680479116963 1103 36-37 0.140971338961144 1103 38-39 -0.3504658789230959 1103 40-41 -0.4152155733163383 1103 42-43 -0.07987495358394625 1103 44-45 0.04511237359958642 1103 46-47 -0.04631115167410371 1103 48-49 0.1909114189022887 1103 50-51 0.5557801467293544 1103 52-53 0.7783516505084656 1103 54-55 1.150524543974523 1103 56-57 0.8272045445794625 1103 58-59 0.681990695806256 1103 60-61 0.2726703966070154 1103 62-63 0.24977553702533228 1103 64-65 0.6505461316365775 1103 66-67 0.37024218190683555 1103 68-69 -0.009626743334951726 1103 70-71 0.15567170432493072 1103 72-73 0.3214424051589262 1103 74-75 0.4680440408319946 1103 76-77 0.26921917924522276 1103 78-79 0.006330491818211215 1103 80-81 0.12710970334874716 1103 82-83 -0.15179480971253767 1103 84-85 -0.22337649187898734 1103 86-87 -0.30144624937660325 1103 88-89 -0.131757688507129 1103 90-91 0.04353524453820867 1103 92-93 0.5609858898783564 1103 94-95 0.6159107548980423 1103 96-97 0.02019168038340524 1103 98-99 -0.28364812594291067 1103 100-101 0.06198313402434863 1104 1 0.22704097600741235 1104 2 0.348871719731612 1104 3 0.3366250399893147 1104 4 0.1333443237804275 1104 5 0.11317831463640005 1104 6 0.09705086166471943 1104 7 0.04394253822373173 1104 8 0.07585141333429135 1104 9 0.0791546830694827 1104 10-11 0.3240894264953553 1104 12-13 0.26670527731977245 1104 14-15 0.13936461353310392 1104 16-17 -0.06039492083396425 1104 18-19 0.12700971814306428 1104 20-21 0.038926560781440855 1104 22-23 0.14527991814358643 1104 24-25 0.14630811853378844 1104 26-27 0.21805316465434288 1104 28-29 0.12524794164876596 1104 30-31 0.36819375684027733 1104 32-33 0.2311898469192144 1104 34-35 0.5869297110745251 1104 36-37 0.4743935974508844 1104 38-39 0.3576660650854464 1104 40-41 0.2237587534679193 1104 42-43 0.5018524960425736 1104 44-45 0.9470017716285852 1104 46-47 1.2797669350750027 1104 48-49 1.3981959584792065 1104 50-51 1.8349842142706265 1104 52-53 1.8778275151748396 1104 54-55 1.9723969939148809 1104 56-57 2.133541246606949 1104 58-59 2.173657885562079 1104 60-61 1.5033997921251832 1104 62-63 1.4958523683604632 1104 64-65 1.632860487295357 1104 66-67 1.401266599708606 1104 68-69 1.0762121870523451 1104 70-71 1.1351770712196654 1104 72-73 1.0208963000807785 1104 74-75 1.132330651841972 1104 76-77 0.9222541425243627 1104 78-79 0.9150618959987362 1104 80-81 0.8466502218347216 1104 82-83 0.8537760897915767 1104 84-85 0.9147033524496813 1104 86-87 0.4840348880284573 1104 88-89 1.1181481554449135 1104 90-91 0.8136375607769892 1104 92-93 0.38773876163782006 1104 94-95 0.06316767536756629 1104 96-97 0.16155129371589894 1104 98-99 0.5658277387234634 1104 100-101 1.1295400984333632 1105 1 0.3741360705538632 1105 2 0.6295650603728831 1105 3 0.3401876598851814 1105 4 0.2598173300835285 1105 5 0.2380755467547857 1105 6 0.27566143990532765 1105 7 0.3058636124906542 1105 8 0.31160093375084585 1105 9 0.4885134114882206 1105 10-11 0.43309189292450867 1105 12-13 0.36402646120576776 1105 14-15 0.4832602013653826 1105 16-17 0.4657766330163966 1105 18-19 0.6234128422866618 1105 20-21 0.4182770677696581 1105 22-23 0.18083760518112513 1105 24-25 0.2133127773444201 1105 26-27 0.5461224987184679 1105 28-29 0.39771985176112423 1105 30-31 0.6982499366155608 1105 32-33 0.4512843933702939 1105 34-35 0.6949387546480992 1105 36-37 0.7765654253489345 1105 38-39 0.44960906316710947 1105 40-41 0.5241835273785753 1105 42-43 0.5616634031404075 1105 44-45 1.1337995244375705 1105 46-47 1.2471552606436518 1105 48-49 1.0686536181119877 1105 50-51 1.0647731975537127 1105 52-53 1.3338428618451665 1105 54-55 1.406008942393921 1105 56-57 1.5248813397831675 1105 58-59 1.7384016509772664 1105 60-61 1.7821062870553028 1105 62-63 1.7011483398595075 1105 64-65 1.5943225316910805 1105 66-67 1.3387494717283381 1105 68-69 1.3909216965069895 1105 70-71 0.9957895677940698 1105 72-73 0.5137710602890522 1105 74-75 1.203685817640821 1105 76-77 1.275946935070266 1105 78-79 1.709457620854863 1105 80-81 1.5519461933337588 1105 82-83 1.597438737092208 1105 84-85 1.6770697542035826 1105 86-87 1.196935682766739 1105 88-89 1.162441112419426 1105 90-91 0.8311766125720013 1105 92-93 1.563608863035462 1105 94-95 1.8364959844933537 1105 96-97 1.6508634340283166 1105 98-99 1.5950138171011012 1105 100-101 2.3935507862930514 1106 1 0.49451725939569613 1106 2 0.4623750501494257 1106 3 0.4048145037584092 1106 4 0.14538765285261945 1106 5 0.3109176673915641 1106 6 0.46497792887860356 1106 7 0.36965067251693995 1106 8 0.34500972592260126 1106 9 -0.07162241442093631 1106 10-11 0.28864907125078787 1106 12-13 0.1467597750658527 1106 14-15 0.7105312689862444 1106 16-17 0.45124472123021775 1106 18-19 0.2739349687654098 1106 20-21 0.6849266953437692 1106 22-23 0.9565057810245605 1106 24-25 0.7172561101909238 1106 26-27 0.8458705635643824 1106 28-29 0.43664200744974835 1106 30-31 0.31875891865148986 1106 32-33 0.22244329507906713 1106 34-35 0.6895166719841583 1106 36-37 0.8846236990454415 1106 38-39 0.9942636564196334 1106 40-41 0.7695099477086487 1106 42-43 0.6091659590042937 1106 44-45 0.3501168897070315 1106 46-47 0.7561007842306253 1106 48-49 0.925999901155663 1106 50-51 0.9023663040237082 1106 52-53 1.2153603147442418 1106 54-55 0.8272883348285625 1106 56-57 0.6914871525478823 1106 58-59 0.35085966178491645 1106 60-61 0.3585266171268664 1106 62-63 0.5588780024854749 1106 64-65 0.3794291477272971 1106 66-67 0.1742431185809714 1106 68-69 0.40565442616961533 1106 70-71 0.7079080138288276 1106 72-73 0.18391857014738378 1106 74-75 0.40466799961832933 1106 76-77 0.6038460149592701 1106 78-79 0.7701046195404189 1106 80-81 0.8542866344028432 1106 82-83 1.0182617252622101 1106 84-85 0.9648782834864811 1106 86-87 0.8559651847384053 1106 88-89 0.3790140640770865 1106 90-91 -0.14844950105440802 1106 92-93 0.10556154304130416 1106 94-95 -0.6305516275995338 1106 96-97 -0.0430170975913704 1106 98-99 0.4198276041611244 1106 100-101 0.1805560440701761 1107 1 0.21589612328952867 1107 2 0.09742926604196356 1107 3 0.22423512198142248 1107 4 0.21242115341905787 1107 5 0.06592656850824596 1107 6 0.050315362120230134 1107 7 0.14136130530541635 1107 8 0.2362218961766871 1107 9 0.1923037872298181 1107 10-11 0.05280184678080957 1107 12-13 0.2505953471648965 1107 14-15 0.3139622448723003 1107 16-17 0.17124795799991688 1107 18-19 -0.0038284233692351677 1107 20-21 -0.3481369071809084 1107 22-23 -0.12858889448926902 1107 24-25 -0.23744529780390167 1107 26-27 -0.057091080714620546 1107 28-29 0.10312525716652488 1107 30-31 -0.1564797931141655 1107 32-33 0.1025533452489995 1107 34-35 0.257974351220291 1107 36-37 0.5188363548149866 1107 38-39 0.7642102497195182 1107 40-41 0.5338407455723839 1107 42-43 0.8672336074910945 1107 44-45 0.9412319568700056 1107 46-47 1.0361137313094417 1107 48-49 1.1888579688041432 1107 50-51 1.6385372054640683 1107 52-53 1.4554315236777278 1107 54-55 1.0004716978322925 1107 56-57 1.2100823944964212 1107 58-59 1.2895552435942648 1107 60-61 1.2163515078858929 1107 62-63 1.0940455053176485 1107 64-65 1.690482716265901 1107 66-67 2.131679596975534 1107 68-69 1.948881485564339 1107 70-71 2.1604731538191153 1107 72-73 1.494324784745217 1107 74-75 1.1088110648392409 1107 76-77 1.5371038236661825 1107 78-79 2.011600007143592 1107 80-81 2.3786271425756844 1107 82-83 2.2809094028799457 1107 84-85 2.2038166230236236 1107 86-87 1.6099383195498653 1107 88-89 1.833650736677832 1107 90-91 2.5031607918732526 1107 92-93 2.0151909329102153 1107 94-95 2.3447194518631633 1107 96-97 2.5311534801720192 1107 98-99 3.152891206685805 1107 100-101 2.4044110367874474 1108 1 0.06411962769280777 1108 2 0.05111891074284358 1108 3 0.38156886021403835 1108 4 -0.0295770245341771 1108 5 0.0682344944116764 1108 6 0.07457895155235406 1108 7 0.2293357966506946 1108 8 0.13203118861519414 1108 9 0.3319636718335275 1108 10-11 0.21453886469760164 1108 12-13 0.033559209904041154 1108 14-15 -0.2876016786017317 1108 16-17 0.33286350613231974 1108 18-19 0.09611084173856455 1108 20-21 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-1.6601650682522369 2316 94-95 -1.7296920564193137 2316 96-97 -1.4805291702203078 2316 98-99 -1.0360509612886197 2316 100-101 -1.42552212217797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 9.0 6 24.0 7 82.0 8 136.0 9 244.0 10 472.0 11 819.0 12 1205.0 13 1636.0 14 2000.0 15 2898.0 16 3873.0 17 5325.0 18 4278.0 19 2056.0 20 1407.0 21 1448.0 22 1466.0 23 1643.0 24 1896.0 25 2039.0 26 2323.0 27 2952.0 28 3655.0 29 4461.0 30 5363.0 31 6758.0 32 8271.0 33 10366.0 34 14199.0 35 20062.0 36 25569.0 37 27419.0 38 11618.0 39 388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.52662363517068 20.645023805651668 16.492914383772902 15.335438175404754 2 16.171663423960887 25.979399940565965 34.392679521612116 23.456257113861025 3 25.21739617965811 26.31124517130987 33.9367911146495 14.534567534382518 4 11.73182478232349 18.80511995335303 40.357477251192805 29.105578013130675 5 9.724044359224498 41.86878370953454 33.51835032125677 14.88882160998419 6 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40.62181104569667 16.637790860667227 28-29 17.247836556656004 29.288551621285986 36.48881625238631 16.974795569671706 30-31 18.94765761447006 24.573812267142124 35.871817083392905 20.60671303499491 32-33 18.488085225679843 25.506027474067842 37.46481637230165 18.54107092795066 34-35 21.351240735134166 24.353842186115877 36.050560096881625 18.244356981868336 36-37 20.31007317278309 23.145588606352856 38.4678572429841 18.076480977879953 38-39 18.93535418103521 25.984210024615763 37.07335946305112 18.00707633129791 40-41 18.49370367463192 27.204274548940894 35.58208771123246 18.71993406519472 42-43 17.825545800114373 27.815061504132043 32.60952445027529 21.749868245478297 44-45 20.29468160258351 24.069869142530358 33.39584664894989 22.23960260593624 46-47 22.642498521261594 27.667686131816566 32.32592235431974 17.363892992602104 48-49 21.185675678706392 26.500947553741433 32.082263363871846 20.23111340368032 50-51 16.938090659071094 28.297528168007197 34.630598908323265 20.133782264598445 52-53 20.69274845538848 30.443984705262334 27.963916080779537 20.89935075856965 54-55 19.34731477302354 26.050219925934442 30.254183358517118 24.348281942524903 56-57 22.76121913476418 26.442340039023076 28.323970037453183 22.472470788759562 58-59 25.36330608537693 25.75856965048609 27.60117065294177 21.27695361119521 60-61 21.603278771245716 27.284781578777697 32.31535185958696 18.796587790389633 62-63 21.120150714041415 26.960319818784306 31.239802412097497 20.67972705507679 64-65 20.40513113779841 30.964555230374184 29.388827216559584 19.241486415267826 66-67 22.42480818342626 27.174738801472298 27.142500721852652 23.257952293248785 68-69 21.01210182690771 27.207945660918973 29.660829270890858 22.119123241282452 70-71 23.897186620468485 28.159865890716635 28.17107904149987 19.77186844731501 72-73 22.487413238245814 24.86095693028784 32.857335082584854 19.794294748881487 74-75 19.144212332223233 32.14277704892298 28.980388199280117 19.732622419573676 76-77 20.01631513438961 27.485675199874414 29.29239411982373 23.205615545912245 78-79 19.85798504722151 28.197228662015068 30.845501972118424 21.099284318644997 80-81 21.550675315792127 27.086078234591643 29.659230437147134 21.704016012469094 82-83 19.63902236625941 26.7778344706166 32.00690780436111 21.576235358762872 84-85 18.906765572479063 28.25543939916401 29.97059178083728 22.867203247519647 86-87 19.065880618186203 28.822244835599808 28.524813509717678 23.58706103649631 88-89 18.696807615972013 28.48925219497427 27.074432894899136 25.739507294154585 90-91 19.645495244209094 24.83972157668555 29.43964925166025 26.075133927445105 92-93 21.2107960215741 27.651910160235925 30.270741525661293 20.866552292528677 94-95 20.074903847232033 26.705239905361005 30.443424047723173 22.77643219968379 96-97 16.028907988943647 27.84973003885422 32.727532672867646 23.393829299334488 98-99 19.097611572101368 28.747477012783136 27.722863870823055 24.43204754429244 100-101 18.45852816182819 26.33856987474911 29.780726836433768 25.422175126988932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1970.0 1 1254.5 2 498.5 3 427.0 4 324.0 5 285.0 6 434.5 7 761.5 8 1086.0 9 1138.5 10 847.5 11 539.0 12 451.5 13 427.0 14 433.0 15 453.5 16 466.5 17 472.5 18 507.5 19 582.0 20 649.5 21 719.0 22 804.0 23 902.0 24 974.0 25 1215.0 26 1831.5 27 2550.5 28 2662.0 29 2710.5 30 3075.0 31 3264.5 32 3519.5 33 3816.0 34 4353.5 35 4696.0 36 4687.5 37 4658.0 38 3745.0 39 2564.0 40 2180.0 41 2190.0 42 2258.0 43 2532.5 44 2861.5 45 2905.5 46 3626.5 47 9699.5 48 22320.0 49 21412.5 50 8674.5 51 3099.0 52 1805.0 53 1513.5 54 1395.0 55 1440.5 56 1777.0 57 2052.0 58 2381.0 59 2681.0 60 2820.0 61 2898.5 62 2514.5 63 1566.0 64 860.0 65 669.0 66 503.5 67 363.5 68 295.0 69 235.5 70 182.5 71 157.0 72 131.0 73 157.0 74 141.5 75 117.0 76 98.0 77 44.5 78 15.0 79 6.5 80 3.5 81 3.0 82 1.5 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02410827418396295 2 0.007288548009105078 3 5.606575391619291E-4 4 5.606575391619291E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011773808322400511 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0011213150783238581 22-23 0.0016819726174857872 24-25 0.011773808322400511 26-27 0.006727890469943149 28-29 2.8032876958096454E-4 30-31 0.004204931543714469 32-33 0.006727890469943149 34-35 0.0 36-37 0.009531178165752795 38-39 0.011773808322400511 40-41 0.0022426301566477163 42-43 0.0 44-45 0.0 46-47 2.8032876958096454E-4 48-49 0.004485260313295433 50-51 0.007008219239524114 52-53 0.0 54-55 0.003644274004552539 56-57 0.0033639452349715744 58-59 0.0 60-61 0.0014016438479048227 62-63 0.006167232930781221 64-65 0.0 66-67 8.409863087428936E-4 68-69 2.8032876958096454E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.8032876958096454E-4 80-81 5.606575391619291E-4 82-83 0.007288548009105078 84-85 0.0058869041612002555 86-87 8.409863087428936E-4 88-89 0.0 90-91 2.8032876958096454E-4 92-93 0.0 94-95 0.0 96-97 0.0016819726174857872 98-99 0.0011213150783238581 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 178362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.474596606900572 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95351805797038 19.348852334017337 2 10.608094724563681 5.404738677520997 3 3.8977045139423816 2.9787735055673297 4 2.0732002552985453 2.112557607562149 5 1.3051037700552413 1.6623496036151197 6 0.8143143253295771 1.2446597369394825 7 0.6558531593194974 1.1695316266917841 8 0.48418689614191074 0.9867572689249952 9 0.41596056077645965 0.9536784741144414 >10 3.061381693333627 15.690001233446585 >50 0.41375971124854194 7.512250367230688 >100 0.25089684618262653 11.352754510489902 >500 0.019807645751259987 3.70258238862538 >1k 0.04401699055835553 21.82415536941725 >5k 0.0022008495279177763 4.056357295836557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7235 4.056357295836557 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3696 2.0721902647424897 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3079 1.7262645630795799 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 1.5333983696078761 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 1.4341619851762146 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2289 1.2833451071416557 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 1.272131956358417 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2237 1.2541909151052355 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1938 1.0865543108958187 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1922 1.0775837902692276 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1896 1.0630066942510177 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1869 1.0478689406936454 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1718 0.9632096522801942 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1637 0.917796391608078 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1504 0.8432289388995413 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1496 0.838743678586246 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1322 0.7411892667720703 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1282 0.7187629652055931 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1252 0.7019432390307352 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1207 0.6767136497684485 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1020 0.5718706899451677 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 935 0.5242147991164037 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 921 0.5163655935681367 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 777 0.43563090792881887 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 744 0.4171292091364753 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 730 0.40928000358820826 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 672 0.37676186631681635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 635 0.356017537367825 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 626 0.3509716195153676 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 564 0.316210852087328 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.2427647144571153 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 418 0.23435485136968637 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 391 0.21921709781231427 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 390 0.21865644027315237 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 349 0.19566948116751326 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 341 0.19118422085421782 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 330 0.1850169879234366 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 330 0.1850169879234366 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 323 0.18109238514930312 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 302 0.1693185768269026 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 293 0.16427265897444523 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 270 0.15137753557372086 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 270 0.15137753557372086 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 269 0.15081687803455895 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 259 0.14521030264293963 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.1446496451037777 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 257 0.1440889875646158 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 246 0.13792175463383455 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 244 0.13680043955551072 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 243 0.13623978201634876 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 239 0.13399715185970104 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 238 0.13343649432053914 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 235 0.13175452170305332 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 229 0.12839057646808177 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 227 0.1272692613897579 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 222 0.12446597369394827 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 222 0.12446597369394827 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 211 0.11829874076316703 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 207 0.11605611060651934 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 206 0.1154954530673574 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 203 0.11381348044987162 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 200 0.11213150783238582 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 199 0.1115708502932239 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 199 0.1115708502932239 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.11101019275406197 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 198 0.11101019275406197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 197 0.11044953521490003 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 193 0.10820690505825231 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 192 0.10764624751909038 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.10764624751909038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 191 0.10708558997992847 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 189 0.1059642749016046 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 189 0.1059642749016046 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 188 0.10540361736244266 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 187 0.10484295982328075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 184 0.10316098720579495 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 184 0.10316098720579495 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 179 0.10035769950998531 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.8032876958096454E-4 0.0 18-19 0.0 0.0 0.0 5.606575391619291E-4 0.0 20-21 0.0 0.0 0.0 8.409863087428936E-4 0.0 22-23 0.0 0.0 0.0 0.0022426301566477163 0.0 24-25 0.0 0.0 0.0 0.005045917852457362 0.0 26-27 0.0 0.0 0.0 0.008690191857009902 0.0 28-29 0.0 0.0 0.0 0.02466893172312488 0.0 30-31 0.0 0.0 0.0 0.04064767158923986 0.0 32-33 0.0 0.0 0.0 0.05718706899451677 0.0 34-35 0.0 0.0 0.0 0.08185600071764165 0.0 36-37 0.0 0.0 0.0 0.11016920644531908 0.0 38-39 0.0 0.0 0.0 0.15922674112198787 0.0 40-41 0.0 0.0 0.0 0.20912526210739957 0.0 42-43 0.0 0.0 0.0 0.2626680570973638 0.0 44-45 0.0 0.0 0.0 0.3010730985299559 0.0 46-47 0.0 0.0 0.0 0.35489622228950113 0.0 48-49 0.0 0.0 0.0 0.3896569897175407 0.0 50-51 0.0 0.0 0.0 0.41965216806270395 0.0 52-53 0.0 0.0 0.0 0.47515726443973494 0.0 54-55 0.0 0.0 0.0 0.5769166077976251 0.0 56-57 0.0 0.0 0.0 0.7568876778686043 0.0 58-59 0.0 0.0 0.0 0.925365268386764 0.0 60-61 0.0 0.0 0.0 1.1370134894203923 0.0 62-63 0.0 0.0 0.0 1.28166313452417 0.0 64-65 0.0 0.0 0.0 1.4535046702773011 0.0 66-67 0.0 0.0 0.0 1.739720344019466 0.0 68-69 0.0 0.0 0.0 2.1473183749901885 0.0 70-71 0.0 0.0 0.0 2.5195949809937095 0.0 72-73 0.0 0.0 0.0 2.8655206826566197 0.0 74-75 0.0 0.0 0.0 3.299189289198372 0.0 76-77 0.0 0.0 0.0 3.6260526345297768 0.0 78-79 0.0 0.0 0.0 3.9784258978930493 0.0 80-81 0.0 0.0 0.0 4.274733407340129 0.0 82-83 0.0 0.0 0.0 4.605241026676086 0.0 84-85 0.0 0.0 0.0 5.221964319754208 0.0 86-87 0.0 0.0 0.0 5.955584709747592 0.0 88-89 0.0 0.0 0.0 6.7864791827855715 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGGC 20 0.0020718754 71.24411 3 CATTGTC 20 0.0020718754 71.24411 9 GCATTGT 20 0.0020718754 71.24411 8 TAATGGG 20 0.0020718754 71.24411 9 CCTGTGT 25 0.005015982 56.995293 8 TATTAGG 25 0.005015982 56.995293 1 GGCATTG 25 0.005015982 56.995293 7 AGGACCG 45 9.607088E-4 42.218735 5 GGTGAGA 60 8.266614E-5 39.580067 9 TGGGTAC 50 0.0016128119 37.99686 6 ACTTAGA 50 0.0016128119 37.99686 1 GGGCATT 50 0.0016128119 37.99686 6 CATTCCG 70 2.0474022E-4 33.925766 9 TTAGGCA 60 0.0039436803 31.664053 4 GATTGAT 60 0.0039436803 31.664053 1 TAGGCAT 60 0.0039436803 31.664053 5 ATACCTT 75 3.0695935E-4 31.664051 6 TAATACC 80 4.480769E-4 29.685045 4 GAACTGG 65 0.0058333725 29.228352 6 AGGGCAT 65 0.0058333725 29.228352 5 >>END_MODULE