##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936900_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179809 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772452991785727 33.0 31.0 34.0 30.0 34.0 2 31.77716354576245 33.0 31.0 34.0 30.0 34.0 3 31.7424767392066 33.0 31.0 34.0 30.0 34.0 4 35.35186225383602 37.0 35.0 37.0 33.0 37.0 5 35.40941777107931 37.0 35.0 37.0 33.0 37.0 6 35.570294034225206 37.0 35.0 37.0 33.0 37.0 7 35.554315968611135 37.0 35.0 37.0 33.0 37.0 8 35.54295947366372 37.0 35.0 37.0 33.0 37.0 9 37.0807857226279 39.0 37.0 39.0 33.0 39.0 10-11 36.95047244576189 39.0 37.0 39.0 33.0 39.0 12-13 36.90446807445679 39.0 37.0 39.0 33.0 39.0 14-15 37.7872103176148 40.0 37.0 41.0 33.0 41.0 16-17 37.787343792579904 40.0 37.0 41.0 32.5 41.0 18-19 37.85573302782397 40.0 37.0 41.0 33.0 41.0 20-21 37.804731687512856 40.0 37.0 41.0 33.0 41.0 22-23 37.67864233714664 40.0 37.0 41.0 32.5 41.0 24-25 37.52309394969106 40.0 37.0 41.0 32.0 41.0 26-27 37.40517716020889 39.5 36.5 41.0 32.5 41.0 28-29 37.35416469698402 39.0 36.0 41.0 32.0 41.0 30-31 37.15714174485148 39.0 36.0 41.0 32.0 41.0 32-33 36.73560277850386 39.0 35.0 41.0 31.0 41.0 34-35 36.39867581711705 38.0 35.0 40.5 31.0 41.0 36-37 36.02584687084629 38.0 35.0 40.0 30.5 41.0 38-39 35.694703824613896 38.0 35.0 40.0 30.0 41.0 40-41 35.525841309389406 38.0 35.0 40.0 29.5 41.0 42-43 35.06658732321519 38.0 35.0 40.0 27.0 41.0 44-45 34.49136583819497 38.0 34.5 40.0 24.0 41.0 46-47 33.65352957860841 38.0 33.5 40.0 17.5 41.0 48-49 32.93656602283534 38.0 33.0 40.0 5.0 41.0 50-51 31.975490659533172 36.0 32.0 39.5 2.0 40.5 52-53 32.1340533566173 37.0 32.5 39.5 2.0 40.5 54-55 32.447424767392064 37.0 33.0 40.0 2.0 41.0 56-57 32.18403416959107 37.0 32.5 40.0 2.0 41.0 58-59 31.95193788964957 36.0 32.0 40.0 2.0 41.0 60-61 31.421330411714653 36.0 31.0 39.5 2.0 41.0 62-63 31.16627643777564 35.0 31.0 39.0 2.0 41.0 64-65 30.81673887291515 35.0 30.5 39.0 2.0 40.5 66-67 30.284543599041207 35.0 30.0 38.5 2.0 40.0 68-69 29.81863254898253 35.0 29.5 37.0 2.0 39.0 70-71 29.238416875684756 35.0 29.0 37.0 2.0 39.0 72-73 28.8359231184201 34.5 28.5 36.0 2.0 38.5 74-75 28.536157811900406 34.0 29.0 35.5 2.0 37.0 76-77 28.18477106262757 34.0 28.0 35.0 2.0 37.0 78-79 27.69423666223604 34.0 26.5 35.0 2.0 36.5 80-81 27.205017546396455 34.0 26.0 35.0 2.0 36.0 82-83 26.788355977731925 33.5 24.5 35.0 2.0 36.0 84-85 26.416667130121404 33.0 24.0 35.0 2.0 35.0 86-87 26.2633766941588 33.0 24.0 35.0 2.0 35.0 88-89 25.781006512466007 33.0 21.5 35.0 2.0 35.0 90-91 25.303897468980974 33.0 19.0 35.0 2.0 35.0 92-93 24.906878966013934 33.0 15.0 35.0 2.0 35.0 94-95 24.421614046015495 32.0 4.5 35.0 2.0 35.0 96-97 23.902638355143516 32.0 2.0 35.0 2.0 35.0 98-99 23.450135977620697 32.0 2.0 35.0 2.0 35.0 100-101 21.467854779237967 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5381941183429326 1101 2 0.5750177298223633 1101 3 0.8143648286197909 1101 4 0.7636472736313422 1101 5 0.6908495012449052 1101 6 0.6021493538623943 1101 7 0.6636176540662291 1101 8 0.617904629159014 1101 9 0.6048629089227546 1101 10-11 0.7873789286887387 1101 12-13 0.7976991117110259 1101 14-15 0.5892305210718192 1101 16-17 0.8109840022973742 1101 18-19 0.8851367203427856 1101 20-21 0.9247947579676818 1101 22-23 1.1190812305293463 1101 24-25 0.8466518777617082 1101 26-27 0.7998242300249885 1101 28-29 0.9361871120849798 1101 30-31 1.1923891408718532 1101 32-33 1.0021195631932756 1101 34-35 1.1749697211258692 1101 36-37 0.7603492444863278 1101 38-39 0.8264592164992663 1101 40-41 0.9601268311935343 1101 42-43 0.801765943935969 1101 44-45 0.7604766822445157 1101 46-47 1.2438645864454259 1101 48-49 0.8344141387546742 1101 50-51 0.9555021714985017 1101 52-53 0.7053030781258798 1101 54-55 1.139656964438295 1101 56-57 1.430380886378181 1101 58-59 1.2726070985219664 1101 60-61 1.0928426818584676 1101 62-63 1.0104867061692424 1101 64-65 1.1269215685469298 1101 66-67 1.1104458796486192 1101 68-69 0.8312584428747378 1101 70-71 0.479949492193807 1101 72-73 0.02298626677964677 1101 74-75 0.2011721206476551 1101 76-77 0.29568389101791226 1101 78-79 0.8902675416844552 1101 80-81 0.5725967819357614 1101 82-83 0.45890137518359353 1101 84-85 0.44087568326147064 1101 86-87 0.7583562207571433 1101 88-89 0.7733994600180729 1101 90-91 1.122143874215066 1101 92-93 1.1802647714302381 1101 94-95 0.944936647423603 1101 96-97 0.9501837511981854 1101 98-99 1.3848401270280597 1101 100-101 1.7904801683249154 1102 1 0.43174436724272525 1102 2 0.4204834249381548 1102 3 0.17328554141107944 1102 4 0.01727255319269716 1102 5 0.414426443745441 1102 6 0.3742437193482928 1102 7 0.28525389964735837 1102 8 0.33581819960847525 1102 9 0.04281832339501079 1102 10-11 -0.012274775950182004 1102 12-13 -0.3606227986850499 1102 14-15 -0.12509384224973275 1102 16-17 -0.13716187462348017 1102 18-19 -0.3483240689220537 1102 20-21 -0.031521525128781036 1102 22-23 0.09709508234379172 1102 24-25 0.0750264539106098 1102 26-27 0.19880338015845922 1102 28-29 0.26047828876775725 1102 30-31 0.25194472849180016 1102 32-33 0.3502726546008148 1102 34-35 0.12457653160129922 1102 36-37 -0.03290160321585489 1102 38-39 0.18842804991677298 1102 40-41 -0.33799379502391247 1102 42-43 0.16891117155302737 1102 44-45 0.06350315941067208 1102 46-47 0.23753387437559326 1102 48-49 -1.2268729256706905 1102 50-51 -1.5535520092965314 1102 52-53 -1.2651175264481012 1102 54-55 -1.4255078865825546 1102 56-57 -1.089549133100931 1102 58-59 -1.2737640146733291 1102 60-61 -1.2168752317119846 1102 62-63 -1.6057206381782088 1102 64-65 -1.2406926007049286 1102 66-67 -1.616107038611247 1102 68-69 -0.8968275538065988 1102 70-71 -0.7204328756007214 1102 72-73 -0.41747618181713975 1102 74-75 -0.6015657770509222 1102 76-77 -1.1442374710771723 1102 78-79 -0.48286570988469535 1102 80-81 -0.7934121640277318 1102 82-83 -0.48255306533296505 1102 84-85 -1.0300679715974823 1102 86-87 -0.6332280805198209 1102 88-89 -0.45564245614959376 1102 90-91 -0.7461917437057188 1102 92-93 -0.7081271194867256 1102 94-95 -0.34256064714388756 1102 96-97 -0.7048014591041039 1102 98-99 -0.800391818718996 1102 100-101 -0.44949169517699517 1103 1 0.2774242295490943 1103 2 0.3877123406007996 1103 3 0.22209793383792942 1103 4 0.27327943787427955 1103 5 0.14850561758365188 1103 6 -0.022658173939142046 1103 7 0.54628660188488 1103 8 0.20996622026252254 1103 9 0.06997839224182911 1103 10-11 0.3013224701771904 1103 12-13 0.31913623745952435 1103 14-15 0.5299319753062122 1103 16-17 0.8231995711596554 1103 18-19 0.7424848811640032 1103 20-21 0.8447263234082314 1103 22-23 0.8659246864745995 1103 24-25 0.7221865227574256 1103 26-27 0.8264281650121603 1103 28-29 0.5095660684579428 1103 30-31 0.8404473102473915 1103 32-33 0.7229921038263001 1103 34-35 1.4202391822037086 1103 36-37 0.619989963049207 1103 38-39 0.6510614406224491 1103 40-41 0.2538650690036306 1103 42-43 0.8213724452191187 1103 44-45 0.5009386155208233 1103 46-47 1.2119056471810978 1103 48-49 0.9652096905083098 1103 50-51 0.7613533263316938 1103 52-53 0.16655200883588606 1103 54-55 0.5341650910422331 1103 56-57 0.8473355484825404 1103 58-59 0.7016404603697026 1103 60-61 0.9945877631245104 1103 62-63 1.4123344392744634 1103 64-65 0.8680165214981699 1103 66-67 0.8096588822149151 1103 68-69 1.0535338919765316 1103 70-71 1.4777082603717346 1103 72-73 1.379752733845514 1103 74-75 0.9899488528974416 1103 76-77 1.0797728559452047 1103 78-79 0.9218158735920703 1103 80-81 1.2472246690187383 1103 82-83 1.297481358074954 1103 84-85 0.49466524699115766 1103 86-87 0.7213502327331938 1103 88-89 1.1229289724218354 1103 90-91 1.2058564490653616 1103 92-93 1.101890092217232 1103 94-95 1.500916117055766 1103 96-97 1.354871518520678 1103 98-99 0.879057406754324 1103 100-101 0.5772380810708526 1104 1 0.17995106687361684 1104 2 0.11788575100542786 1104 3 0.27976927983297273 1104 4 0.33237109765958195 1104 5 0.46725045655970376 1104 6 0.46916676412856617 1104 7 0.16564250609627607 1104 8 0.40378950680256764 1104 9 0.476518441787654 1104 10-11 0.5876973669567107 1104 12-13 0.5906969311011494 1104 14-15 0.44164130643751065 1104 16-17 0.6974192243388373 1104 18-19 0.5442520157635045 1104 20-21 0.7571127808813856 1104 22-23 0.5520188235513928 1104 24-25 0.690807497158751 1104 26-27 0.5750656546901141 1104 28-29 0.5604991815875877 1104 30-31 0.5518263358460658 1104 32-33 0.480382690118617 1104 34-35 0.3061351359609361 1104 36-37 0.6856547029335971 1104 38-39 0.4795172622574597 1104 40-41 0.6273415347344269 1104 42-43 1.1793575814701498 1104 44-45 1.232647946652122 1104 46-47 0.8323350443735009 1104 48-49 0.9762749258510084 1104 50-51 0.7677447383878473 1104 52-53 0.896279506771485 1104 54-55 0.7489297483502426 1104 56-57 0.6693999580614047 1104 58-59 0.5424662242012417 1104 60-61 1.0782706698131896 1104 62-63 0.23443187940658206 1104 64-65 0.22890834643623847 1104 66-67 0.3107158599192914 1104 68-69 0.054805568277110694 1104 70-71 0.09602370215538869 1104 72-73 0.5472671847125632 1104 74-75 0.6897671848544995 1104 76-77 0.8746531201896275 1104 78-79 1.0066878801981787 1104 80-81 0.5555153378874458 1104 82-83 0.751899194573717 1104 84-85 1.0886206557442542 1104 86-87 1.3798473425597848 1104 88-89 1.6151667924053186 1104 90-91 1.6357986455971592 1104 92-93 0.6070758890793329 1104 94-95 0.17815806585840832 1104 96-97 -0.13151989353547222 1104 98-99 -0.4764834685553403 1104 100-101 -0.05627141521321022 1105 1 0.1337082923071442 1105 2 0.19881060649675675 1105 3 0.308671013937019 1105 4 -0.19364046303405757 1105 5 0.12620999413196188 1105 6 0.17436907626729692 1105 7 0.24078701476679498 1105 8 0.16101494032857744 1105 9 -0.12463762757650443 1105 10-11 0.11370892765035734 1105 12-13 -0.1867597162976935 1105 14-15 0.22776847406756673 1105 16-17 0.026898993124966353 1105 18-19 -0.18407168365845905 1105 20-21 0.135725497644394 1105 22-23 -0.173414702460164 1105 24-25 -0.1357820982169713 1105 26-27 -0.4220441718994792 1105 28-29 -0.22562798604246836 1105 30-31 0.3350633300657222 1105 32-33 0.07286823925156227 1105 34-35 -0.214096077911897 1105 36-37 -0.05711986354038601 1105 38-39 0.39281205994532087 1105 40-41 -0.008496615554584253 1105 42-43 0.6649067144181302 1105 44-45 0.2991219350914207 1105 46-47 -0.09830079377679368 1105 48-49 0.005176659955047569 1105 50-51 -0.3420818512075279 1105 52-53 -0.6210615336909449 1105 54-55 -0.48806447130294117 1105 56-57 -0.3710624696842544 1105 58-59 -0.25811181048083753 1105 60-61 -0.32346343423305157 1105 62-63 -0.3725045367783899 1105 64-65 -1.1352535032747433 1105 66-67 -1.1141396314101861 1105 68-69 -0.8642695762315604 1105 70-71 -1.317271095532476 1105 72-73 -1.46140333551865 1105 74-75 -1.1252617168796064 1105 76-77 -1.183150348018465 1105 78-79 -1.2331965128654012 1105 80-81 -1.0889933326327892 1105 82-83 -1.3926094759465144 1105 84-85 -0.6454833904985229 1105 86-87 -1.1172624840298155 1105 88-89 -1.2576655775368764 1105 90-91 -0.8699817012236544 1105 92-93 -0.8293402958917682 1105 94-95 -0.15132170292771718 1105 96-97 0.09102345906568488 1105 98-99 0.292302658612293 1105 100-101 0.6518210703359202 1106 1 0.09842335312490391 1106 2 -0.04222630970594565 1106 3 0.03720748686159325 1106 4 -0.0743454525726932 1106 5 0.07400956149250248 1106 6 0.17068100222211768 1106 7 0.1713873906666521 1106 8 0.33382248669547465 1106 9 0.4748162537668037 1106 10-11 0.25995524844186946 1106 12-13 0.37361476506689684 1106 14-15 0.1762772864367932 1106 16-17 0.5191504914050498 1106 18-19 0.4709160818651412 1106 20-21 0.19170265995511215 1106 22-23 0.3239965532581266 1106 24-25 -0.16475475682189256 1106 26-27 0.33308898885089633 1106 28-29 -0.0372525117348701 1106 30-31 0.042357914980080125 1106 32-33 0.3222033220559197 1106 34-35 0.7304232312834671 1106 36-37 0.642443950779267 1106 38-39 0.4850316422000134 1106 40-41 0.994163053227993 1106 42-43 1.002897423308518 1106 44-45 1.191210307019503 1106 46-47 0.8101002133511273 1106 48-49 1.471257017414537 1106 50-51 1.173220109854924 1106 52-53 1.319663314707931 1106 54-55 1.4999670191754504 1106 56-57 0.8706659604019151 1106 58-59 1.4334020729902122 1106 60-61 1.5612310584794606 1106 62-63 1.6505991193796312 1106 64-65 1.5895239711037767 1106 66-67 1.8409296972894147 1106 68-69 1.420527757007207 1106 70-71 1.8599869632639354 1106 72-73 2.5415932246430586 1106 74-75 2.1305097643785977 1106 76-77 1.758212820888236 1106 78-79 2.002166443267793 1106 80-81 1.385296535802265 1106 82-83 1.402915188048663 1106 84-85 1.6972769910752952 1106 86-87 1.948466797150374 1106 88-89 1.9753391389424308 1106 90-91 2.354813504280088 1106 92-93 2.0735640624188107 1106 94-95 1.9977084045228999 1106 96-97 2.226808415979054 1106 98-99 2.4891713418989347 1106 100-101 3.2791837427711172 1107 1 0.2802813724062325 1107 2 0.11461710250556578 1107 3 0.2454312671712593 1107 4 0.36423181882666 1107 5 0.3330294271074621 1107 6 0.22019896891799817 1107 7 -0.13514196954368884 1107 8 0.23591860121489816 1107 9 0.32093077319420615 1107 10-11 0.31858437493909264 1107 12-13 0.5821124279357193 1107 14-15 0.4289795943538266 1107 16-17 0.7031995711596508 1107 18-19 0.5622467859259075 1107 20-21 0.6703215615034637 1107 22-23 0.26282944837936384 1107 24-25 0.35313890370981227 1107 26-27 0.35499959358358524 1107 28-29 0.1484946398865219 1107 30-31 0.07104254834262491 1107 32-33 0.2741825800167703 1107 34-35 0.30214394410847234 1107 36-37 0.7465375820968276 1107 38-39 0.3646328691938763 1107 40-41 0.7487460213845836 1107 42-43 1.0580391118857833 1107 44-45 1.7437957583779706 1107 46-47 1.4852389805144313 1107 48-49 2.171757309555936 1107 50-51 2.1757580882364564 1107 52-53 2.3605996278835093 1107 54-55 2.2784508053279424 1107 56-57 2.3310260246730223 1107 58-59 2.3035452222744652 1107 60-61 2.8661353821721285 1107 62-63 2.940786820226837 1107 64-65 2.618135569117218 1107 66-67 2.6765636441196783 1107 68-69 2.3780577015003423 1107 70-71 2.4249701651336437 1107 72-73 2.4433241624169426 1107 74-75 2.5925679005164888 1107 76-77 2.6291776178499653 1107 78-79 2.324434921211118 1107 80-81 2.716986573780641 1107 82-83 2.4397432628368563 1107 84-85 1.6770461993721106 1107 86-87 1.9280168993998608 1107 88-89 2.0711432581361215 1107 90-91 2.0366897823986996 1107 92-93 2.3556996160267545 1107 94-95 2.1129399265795747 1107 96-97 1.8384429470921084 1107 98-99 1.4637002638971843 1107 100-101 1.994857128689901 1108 1 0.2629068550317193 1108 2 0.292577205465669 1108 3 0.28217515391515136 1108 4 0.3213103259051735 1108 5 -0.028328359250323842 1108 6 0.32097116969020334 1108 7 -0.1658369502386705 1108 8 0.001008691304988929 1108 9 0.29885870112213553 1108 10-11 0.09475876361348412 1108 12-13 0.044001102324394026 1108 14-15 0.44004780542204003 1108 16-17 0.5788752468353238 1108 18-19 0.2450331437122628 1108 20-21 0.5976220763039777 1108 22-23 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-3.1187598434980934 2316 66-67 -2.294858694449257 2316 68-69 -2.3850001394370786 2316 70-71 -1.947676930353765 2316 72-73 -3.3288336535890437 2316 74-75 -3.4448604215976992 2316 76-77 -3.326584660883519 2316 78-79 -3.965424216161111 2316 80-81 -3.934316093883279 2316 82-83 -5.110446839382039 2316 84-85 -4.476718447848036 2316 86-87 -4.699382751560002 2316 88-89 -4.78941208965745 2316 90-91 -4.820530985489608 2316 92-93 -4.810032121694512 2316 94-95 -5.587056956990605 2316 96-97 -6.696616888360396 2316 98-99 -6.492311329221742 2316 100-101 -6.730211432766096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 462.0 3 139.0 4 114.0 5 122.0 6 141.0 7 192.0 8 248.0 9 333.0 10 455.0 11 656.0 12 1091.0 13 1528.0 14 1763.0 15 2390.0 16 3229.0 17 4511.0 18 3659.0 19 1713.0 20 1539.0 21 1507.0 22 1566.0 23 1744.0 24 1858.0 25 2117.0 26 2377.0 27 2762.0 28 3323.0 29 3966.0 30 4870.0 31 5857.0 32 7399.0 33 9378.0 34 13195.0 35 19648.0 36 24886.0 37 27155.0 38 19514.0 39 2402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.08676591672786 20.62442975721566 14.954825644791597 16.33397868126488 2 15.98716087292713 25.14366137636777 34.49041237629545 24.37876537440964 3 24.738431758639678 26.8649078601187 33.127527380535206 15.269133000706415 4 11.723140265954488 18.726979357925778 40.14521782674011 29.404662549379623 5 10.40298341311366 41.43548925748636 32.99343203829456 15.168095291105422 6 24.254030462278436 38.31555624832992 16.813262670348266 20.61715061904338 7 21.670543196412076 39.60681972467365 20.81919049155881 17.903446587355464 8 25.18492132842807 26.512347568611517 20.061668271451712 28.2410628315087 9 23.993121448725297 20.40469923033686 25.914774972313275 29.68740434862457 10-11 25.245908937868165 25.574807794513344 25.17801441923752 24.001268848380967 12-13 30.849195992064267 26.341242170696212 25.127230534215204 17.682331303024316 14-15 24.611022693125285 27.653559782306264 31.233653493005086 16.501764031563365 16-17 18.20387269085244 30.762575116848428 30.04618295125751 20.98736924104162 18-19 17.464879648977345 31.401169148068902 32.96123892968139 18.172712273272364 20-21 21.441806891025642 25.65215455840456 31.247217770655272 21.65882077991453 22-23 19.532975438791688 26.177018719924337 33.220772718422296 21.06923312286167 24-25 22.873208728805782 26.928371399729134 31.622315352720175 18.576104518744906 26-27 20.954089152137037 24.281860904869163 37.47698896026251 17.28706098273129 28-29 17.662222815477282 31.07970567133299 33.30163126602484 17.95644024716489 30-31 18.988545638082208 26.894808412657362 32.87866274009249 21.237983209167936 32-33 18.763313181648805 27.519006913121586 34.3904161777056 19.327263727524013 34-35 21.78185883282073 26.476493456654858 32.47534774556315 19.266299964961263 36-37 20.280428150714837 25.407335515429946 35.554640941286394 18.75759539256882 38-39 19.620193323767253 28.17849634598058 33.48711360274079 18.71419672751137 40-41 18.71664594856906 29.657191960821937 32.72847612134965 18.89768596925935 42-43 18.06732386924542 29.81354541788907 29.476230864420682 22.642899848444824 44-45 20.070465618117506 26.70658049876532 30.42841093635292 22.794542946764253 46-47 22.837303849458717 29.495090501071996 29.930841552908856 17.73676409656043 48-49 21.044552831059622 28.267216880133923 29.913964262078096 20.774266026728363 50-51 16.99429102163739 30.151914996565715 32.63016220394929 20.223631777847608 52-53 20.68933872093573 31.977719130901495 26.05599139021833 21.276950757944444 54-55 19.199385940029035 27.661899914899298 28.75903152063275 24.37968262443892 56-57 22.582726390406826 28.240296560795798 26.307256339876915 22.869720708920465 58-59 25.03865449003882 27.4822300581764 26.20329480861856 21.27582064316622 60-61 21.84350737469689 28.595025694645283 30.772118529064983 18.789348401592846 62-63 20.870343899305304 28.844324799350357 29.621338109249074 20.66399319209527 64-65 20.242541204474858 32.27811317291837 27.946742156063948 19.532603466542827 66-67 21.903916916752657 28.977618810545607 25.645333103411833 23.473131169289903 68-69 20.466476196305024 28.84680118288215 28.288743424182318 22.39797919663051 70-71 23.42471927618327 29.534705142615493 26.498770268315212 20.54180531288603 72-73 21.86279062653812 26.684667599155752 31.46149589417454 19.991045880131587 74-75 18.86967186444072 33.52105390500505 27.562942012420148 20.046332218134086 76-77 19.672418396635738 29.131622277106555 27.831086042320273 23.36487328393743 78-79 19.949044727714718 29.53486625631726 29.295387602181734 21.220701413786294 80-81 21.6589823283406 28.54928781904844 28.033412263591817 21.758317589019143 82-83 19.399996106038145 28.553866008772538 30.624619295693556 21.42151858949576 84-85 19.317195808396555 29.74002714307963 28.33754199390393 22.605235054619886 86-87 19.31171453844485 30.788145823945346 27.472726160076995 22.42741347753281 88-89 19.131610501687312 30.03446961582653 25.402910582088595 25.431009300397555 90-91 19.586444412298174 26.91978145481656 28.0100036720932 25.48377046079206 92-93 21.850728830532994 29.260042283298098 28.734004673417157 20.15522421275175 94-95 20.315372314537996 28.47472710839185 29.015365361885557 22.194535215184594 96-97 16.49483389189415 29.49812963246235 31.223039590605055 22.78399688503845 98-99 19.69585704669237 30.526116683634584 26.209868095309623 23.568158174363425 100-101 19.106835775543026 27.933016857090387 28.73162777496424 24.228519592402346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1683.0 1 1026.0 2 353.0 3 334.0 4 290.0 5 308.5 6 467.5 7 776.5 8 1074.0 9 1100.0 10 797.0 11 558.5 12 522.5 13 518.5 14 547.0 15 526.0 16 514.0 17 518.0 18 546.0 19 596.0 20 650.0 21 721.0 22 788.5 23 837.0 24 972.0 25 1253.5 26 1835.0 27 2542.5 28 2642.0 29 2686.5 30 3049.5 31 3322.5 32 3526.5 33 3723.0 34 4286.5 35 4658.5 36 4713.5 37 4710.5 38 3752.0 39 2552.5 40 2156.5 41 2137.0 42 2193.5 43 2440.5 44 2738.5 45 2713.5 46 3499.0 47 9529.0 48 22065.5 49 21427.0 50 8851.0 51 3270.5 52 2014.5 53 1787.0 54 1657.5 55 1680.0 56 1951.5 57 2184.5 58 2475.0 59 2679.5 60 2956.0 61 3180.5 62 2809.0 63 1749.0 64 918.0 65 728.5 66 571.5 67 400.5 68 286.5 69 216.0 70 180.0 71 156.5 72 133.0 73 174.5 74 146.0 75 114.0 76 99.5 77 42.0 78 23.0 79 16.0 80 5.0 81 3.0 82 3.0 83 3.5 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03392488696338893 2 0.025582701644522796 3 0.015572079261883443 4 0.04393550934602829 5 0.0828657075007369 6 0.09843778676262033 7 0.05283384035281882 8 0.0767481049335684 9 0.06673748255092905 10-11 0.06590326401904242 12-13 0.0642348269552692 14-15 0.059507588607911735 16-17 0.049496966225272374 18-19 0.05867337007602511 20-21 0.05394613172866764 22-23 0.030031867147918067 24-25 0.007507966786979517 26-27 0.002224582751697635 28-29 0.005561456879244087 30-31 0.0047272383473574734 32-33 0.0033368741275464522 34-35 0.00444916550339527 36-37 0.006951821099055109 38-39 0.0038930198154708607 40-41 0.008064112474903925 42-43 0.0036149469715086565 44-45 0.0027807284396220433 46-47 0.002502655595659839 48-49 0.0 50-51 0.002502655595659839 52-53 0.007507966786979517 54-55 0.01223520513433699 56-57 0.008064112474903925 58-59 0.006117602567168495 60-61 0.0027807284396220433 62-63 0.010010622382639356 64-65 0.002502655595659839 66-67 0.020855463297165325 68-69 0.04365743650206608 70-71 0.05283384035281882 72-73 0.0019465099077354304 74-75 0.011400986602450378 76-77 0.020577390453203122 78-79 0.024192337424711777 80-81 0.0636786812673448 82-83 0.024192337424711777 84-85 0.011679059446412583 86-87 0.0322564498996157 88-89 0.04755045631753694 90-91 0.04171092659433065 92-93 0.0383740524667842 94-95 0.011957132290374786 96-97 0.017518589169618876 98-99 0.0322564498996157 100-101 0.06784977392677786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.538109883265022 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7203328217142 21.40271065408294 2 9.928104047176669 5.468024403672786 3 3.6735600613942965 3.034887019003498 4 1.7893206236368042 1.9709803180041043 5 1.1733581064706358 1.6156032234204074 6 0.8219565393004282 1.3581077699114061 7 0.5937474755634543 1.1445478257484332 8 0.4281444381614024 0.9432230867197972 9 0.3554406656434284 0.8809347696722634 >10 2.827368931254544 15.479202932000065 >50 0.41400759350512967 8.052989561145438 >100 0.2140722190807012 10.645184612561106 >500 0.016156393892883106 3.025432542308783 >1k 0.04241053396881816 21.495030838278396 >5k 0.0020195492366103882 3.483140443470571 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6263 3.483140443470571 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4486 2.4948695560288976 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3306 1.838617644278095 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2286 1.2713490425951983 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2119 1.178472712711822 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2088 1.1612321963861654 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2051 1.1406548059329622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2032 1.1300880378623983 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1944 1.0811472173250505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1912 1.0633505553114695 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1780 0.9899393245054474 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1777 0.9882708874416742 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1728 0.9610197487333781 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1707 0.9493406892869656 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1380 0.767481049335684 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1291 0.7179840831104116 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1259 0.7001874210968305 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1170 0.6506904548715582 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1106 0.615097130844396 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1100 0.6117602567168495 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1079 0.600081197270437 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1049 0.5833968266327048 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 956 0.5316752776557347 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 739 0.410991663376138 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 737 0.40987937200028923 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 666 0.3703930281576562 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 649 0.36093855146294124 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 604 0.3359119955063428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 583 0.32423293605993025 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 506 0.2814097180897508 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 494 0.27473596983465787 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 469 0.2608323276365477 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 418 0.23246889755240285 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 390 0.21689681829051938 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 338 0.18797724251845013 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 329 0.18297193132713047 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.1729613089444911 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 308 0.17129287188071787 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 304 0.16906828912902025 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 301 0.167399852065247 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 290 0.1612822494980785 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 271 0.15071548142751473 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 270 0.15015933573959034 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 261 0.14515402454827067 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 256 0.14237329610864863 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 255 0.1418171504207242 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 252 0.140148713356951 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 247 0.13736798491732893 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 244 0.13569954785355573 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 241 0.1340311107897825 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 239 0.1329188194139337 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 233 0.12958194528638722 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 232 0.1290257995984628 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 232 0.1290257995984628 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 222 0.12346434271921873 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 221 0.12290819703129433 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 214 0.11901517721582347 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 208 0.115678303088277 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 207 0.11512215740035259 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 205 0.11400986602450379 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 205 0.11400986602450379 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 199 0.11067299189695733 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 198 0.11011684620903292 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 188 0.10455538932978885 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 187 0.10399924364186443 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 187 0.10399924364186443 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 185 0.1028869522660156 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 181 0.10066236951431796 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7807284396220435E-4 0.0 16-17 0.0 0.0 0.0 5.561456879244087E-4 0.0 18-19 0.0 0.0 0.0 5.561456879244087E-4 0.0 20-21 0.0 0.0 0.0 0.0013903642198110217 0.0 22-23 0.0 0.0 0.0 0.00444916550339527 0.0 24-25 0.0 0.0 0.0 0.006951821099055108 0.0 26-27 0.0 0.0 0.0 0.011400986602450378 0.0 28-29 0.0 0.0 0.0 0.026694993020371614 0.0 30-31 0.0 0.0 0.0 0.04782852916149915 0.0 32-33 0.0 0.0 0.0 0.07368930364998415 0.0 34-35 0.0 0.0 0.0 0.09704742254280932 0.0 36-37 0.0 0.0 0.0 0.11957132290374786 0.0 38-39 0.0 0.0 0.0 0.16100417665411632 0.0 40-41 0.0 0.0 0.0 0.2046616131561824 0.0 42-43 0.0 0.0 0.0 0.2549927979133414 0.0 44-45 0.0 0.0 0.0 0.28446851937333506 0.0 46-47 0.0 0.0 0.0 0.32923824725124995 0.0 48-49 0.0 0.0 0.0 0.36260698852671447 0.0 50-51 0.0 0.0 0.0 0.3948634384263302 0.0 52-53 0.0 0.0 0.0 0.46215706666518364 0.0 54-55 0.0 0.0 0.0 0.5711616214983677 0.0 56-57 0.0 0.0 0.0 0.7496843873221029 0.0 58-59 0.0 0.0 0.0 0.928207153145838 0.0 60-61 0.0 0.0 0.0 1.1209116340116458 0.0 62-63 0.0 0.0 0.0 1.2699586783753873 0.0 64-65 0.0 0.0 0.0 1.4429199873198784 0.0 66-67 0.0 0.0 0.0 1.729613089444911 0.0 68-69 0.0 0.0 0.0 2.1216957994316195 0.0 70-71 0.0 0.0 0.0 2.4640034703490925 0.0 72-73 0.0 0.0 0.0 2.8110383796139238 0.0 74-75 0.0 0.0 0.0 3.2395486321596803 0.0 76-77 0.0 0.0 0.0 3.5704553164747037 0.0 78-79 0.0 0.0 0.0 3.9116506960163284 0.0 80-81 0.0 0.0 0.0 4.188611248602684 0.0 82-83 0.0 0.0 0.0 4.48837377439394 0.0 84-85 0.0 0.0 0.0 5.070658309650796 0.0 86-87 0.0 0.0 0.0 5.7944819224844135 0.0 88-89 0.0 0.0 0.0 6.613406447953106 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCAGC 25 0.0050046924 57.027905 6 CTTTATG 65 2.5684603E-6 43.86762 9 CTATACC 75 6.866634E-6 38.0186 2 CCTTTAT 80 1.0689648E-5 35.64244 8 CCCTTTA 80 1.0689648E-5 35.64244 7 ATGCACC 55 0.002567687 34.562363 4 ATATTAA 55 0.002567687 34.562363 1 CGTCACT 70 2.0405884E-4 33.94518 1 TACCCTT 85 1.6191432E-5 33.545826 5 ATACCTT 100 1.2861874E-6 33.266277 6 ATGGGTG 60 0.003932722 31.68217 5 CATGGGT 165 7.4578566E-11 31.682169 4 CACTACC 110 2.7062033E-6 30.242067 3 TGGGGAG 80 4.4659004E-4 29.702034 6 TGTCCGT 40 4.4893328E-4 29.6855 52-53 CATGCAC 65 0.0058171907 29.245079 3 TGCACCA 65 0.0058171907 29.245079 5 CCACCAC 65 0.0058171907 29.245079 9 TAATACC 115 3.826128E-6 28.927198 4 ACTACCT 115 3.826128E-6 28.927198 4 >>END_MODULE