##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936900_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179809 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85637537609352 31.0 31.0 34.0 30.0 34.0 2 31.924163973994627 31.0 31.0 34.0 30.0 34.0 3 32.00820871035376 33.0 31.0 34.0 30.0 34.0 4 35.730764311018916 37.0 35.0 37.0 35.0 37.0 5 35.58601627282283 37.0 35.0 37.0 33.0 37.0 6 35.76948873526909 37.0 35.0 37.0 33.0 37.0 7 35.726215039291695 37.0 35.0 37.0 33.0 37.0 8 35.709274841637516 37.0 35.0 37.0 33.0 37.0 9 37.27000317003042 39.0 37.0 39.0 34.0 39.0 10-11 37.1702250721599 39.0 37.0 39.0 33.5 39.0 12-13 37.111251383412394 39.0 37.0 39.0 33.0 39.0 14-15 37.929984038618755 39.5 37.0 41.0 33.0 41.0 16-17 38.002858588835934 39.5 37.0 41.0 33.0 41.0 18-19 38.27300079528834 40.0 37.5 41.0 33.5 41.0 20-21 38.3167917067555 40.0 37.5 41.0 34.0 41.0 22-23 38.194036449788385 40.0 37.0 41.0 33.5 41.0 24-25 37.98678597845492 40.0 37.0 41.0 33.0 41.0 26-27 37.65067098977248 39.5 36.5 41.0 33.0 41.0 28-29 37.480142818212656 39.0 36.0 41.0 33.0 41.0 30-31 37.291820765367696 39.0 35.5 41.0 33.0 41.0 32-33 36.85890861970202 39.0 35.0 41.0 32.0 41.0 34-35 36.49352368346412 38.0 35.0 40.0 31.0 41.0 36-37 36.12329193755596 38.0 35.0 40.0 30.5 41.0 38-39 35.765470582673835 38.0 35.0 40.0 30.0 41.0 40-41 35.41764594653215 38.0 35.0 40.0 29.0 41.0 42-43 34.89739946276326 38.0 35.0 40.0 26.5 41.0 44-45 34.255006701555544 38.0 34.0 40.0 23.5 41.0 46-47 33.38503634412071 38.0 33.0 40.0 13.0 41.0 48-49 32.5178606187677 37.0 33.0 40.0 2.0 41.0 50-51 32.00027807284396 37.0 32.0 40.0 2.0 41.0 52-53 31.759836826855164 37.0 31.5 40.0 2.0 41.0 54-55 31.366338726092685 36.5 31.0 40.0 2.0 41.0 56-57 31.006145409851566 35.5 31.0 39.5 2.0 41.0 58-59 30.708529606415695 35.0 30.5 39.0 2.0 40.0 60-61 30.115269536007652 35.0 29.0 39.0 2.0 40.0 62-63 29.806147078288628 35.0 28.5 38.0 2.0 40.0 64-65 29.507855557841932 35.0 28.5 38.0 2.0 40.0 66-67 29.034186275436713 34.5 27.5 37.0 2.0 39.0 68-69 28.529459037089357 34.0 26.5 36.5 2.0 39.0 70-71 27.99462207119777 34.0 26.0 36.0 2.0 38.5 72-73 27.538296192070476 33.5 26.0 35.0 2.0 37.0 74-75 27.193230038540896 33.0 26.0 35.0 2.0 37.0 76-77 25.85484319472329 31.5 24.0 34.0 2.0 35.5 78-79 26.515171654366576 33.0 25.0 35.0 2.0 36.0 80-81 26.30627221106841 33.0 24.0 35.0 2.0 36.0 82-83 25.930373340600305 33.0 23.0 35.0 2.0 35.0 84-85 25.479859183911817 32.0 20.0 35.0 2.0 35.0 86-87 25.29004666062322 32.0 20.0 34.5 2.0 35.0 88-89 24.867070057672308 32.0 18.5 34.0 2.0 35.0 90-91 24.278851447925298 31.0 9.5 34.0 2.0 35.0 92-93 23.83836181726165 31.0 4.0 34.0 2.0 35.0 94-95 23.188363763771555 31.0 2.0 34.0 2.0 35.0 96-97 22.360846787424435 30.0 2.0 34.0 2.0 35.0 98-99 21.643877670194485 30.0 2.0 34.0 2.0 35.0 100-101 18.833451050837276 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25360639148016517 1101 2 0.5748959565347462 1101 3 0.5272346230538361 1101 4 0.5051220814932762 1101 5 0.1692211215961379 1101 6 0.2642313357514823 1101 7 0.5251566413406863 1101 8 0.5523497763195238 1101 9 0.6784257467508681 1101 10-11 0.85189675173023 1101 12-13 0.9399582494658105 1101 14-15 0.9583141709704748 1101 16-17 0.9409443925890315 1101 18-19 0.7457606057773489 1101 20-21 0.7890872719831847 1101 22-23 0.9053564761838047 1101 24-25 1.0777455583807125 1101 26-27 1.0644268633523168 1101 28-29 0.7231037223759458 1101 30-31 1.0514719044082668 1101 32-33 0.9283310688785562 1101 34-35 0.7152639437978152 1101 36-37 1.051800948035634 1101 38-39 1.1468096967341879 1101 40-41 1.1537168666718784 1101 42-43 1.078958021654465 1101 44-45 0.6332408297596359 1101 46-47 0.6189469226753346 1101 48-49 0.6031374298762202 1101 50-51 0.3946967485443942 1101 52-53 0.1535947908655224 1101 54-55 -0.44852210544502213 1101 56-57 0.02253992332174448 1101 58-59 0.052800226453999954 1101 60-61 0.05157224699727081 1101 62-63 -0.15769785716828366 1101 64-65 -0.1876650935723383 1101 66-67 0.06503249277416501 1101 68-69 0.008454275264185185 1101 70-71 0.01167626419092116 1101 72-73 0.06600818273192033 1101 74-75 -0.30686164731127263 1101 76-77 -0.0970154931041769 1101 78-79 0.04690744316715367 1101 80-81 0.17969661701040351 1101 82-83 0.4927425119701887 1101 84-85 0.3665829139710013 1101 86-87 0.43679864966914295 1101 88-89 -0.07309650811108526 1101 90-91 0.11643339473589265 1101 92-93 0.2719505142255123 1101 94-95 0.059002450092894065 1101 96-97 0.06979892457055925 1101 98-99 0.42250200901911583 1101 100-101 0.10565075227803788 1102 1 -0.19159958211481154 1102 2 -0.1932665137054954 1102 3 -0.03578391817695703 1102 4 -0.23436280415710797 1102 5 -0.5913661376203692 1102 6 -0.21751601292466205 1102 7 -0.03955730426208248 1102 8 0.21676792796803568 1102 9 0.1601009505601212 1102 10-11 0.011445124863477929 1102 12-13 0.2594337562835065 1102 14-15 0.4635266376034721 1102 16-17 0.04003536726617796 1102 18-19 -0.09140842964578155 1102 20-21 0.012501888533215322 1102 22-23 0.20087988719311056 1102 24-25 0.11711861948741387 1102 26-27 0.11373787987349715 1102 28-29 0.010041894225018666 1102 30-31 0.2943656350193322 1102 32-33 0.4478282191563139 1102 34-35 0.5804612600087609 1102 36-37 0.7211790592830241 1102 38-39 1.0163176687419764 1102 40-41 1.2650544325040656 1102 42-43 1.1504716209618735 1102 44-45 1.2644182339873211 1102 46-47 0.8956044509921952 1102 48-49 0.931685153705665 1102 50-51 1.24881982768731 1102 52-53 1.2792350765591998 1102 54-55 1.3214980951177075 1102 56-57 1.0462395120960046 1102 58-59 0.46655103808375387 1102 60-61 0.6601141992373698 1102 62-63 0.07147031251644265 1102 64-65 0.4309230313825694 1102 66-67 0.8660569498840474 1102 68-69 1.146593659147026 1102 70-71 0.7822555874720685 1102 72-73 0.4728150509232485 1102 74-75 0.63181521582991 1102 76-77 0.6362649339934308 1102 78-79 0.9775762260832472 1102 80-81 1.552396999378196 1102 82-83 1.085232231326657 1102 84-85 1.0244178465155507 1102 86-87 0.6575078337106994 1102 88-89 0.4734910396848875 1102 90-91 0.3169961246976527 1102 92-93 -0.5258274238741869 1102 94-95 0.3795060212638077 1102 96-97 1.237752174696812 1102 98-99 0.8927422514258687 1102 100-101 0.04806111228092291 1103 1 -0.09934484889622297 1103 2 -0.15584826929929818 1103 3 -0.10311610406336058 1103 4 0.11104167088591765 1103 5 0.07242043725054259 1103 6 0.017148358848139367 1103 7 -0.006614102885151851 1103 8 0.07036517409540011 1103 9 -0.08537237129874597 1103 10-11 -0.4959903312466736 1103 12-13 0.12416697487214634 1103 14-15 -0.001241004393087053 1103 16-17 0.011842596181843135 1103 18-19 0.10724905744543634 1103 20-21 -0.33488193246505915 1103 22-23 -0.33152975136110285 1103 24-25 -0.4438968710461424 1103 26-27 -0.29834473630550207 1103 28-29 -0.43148994742730906 1103 30-31 -0.5946016627431447 1103 32-33 -0.28248159151494434 1103 34-35 -0.25606197579158163 1103 36-37 -0.49067980474451645 1103 38-39 -0.4972279422735113 1103 40-41 -0.29117620432898406 1103 42-43 -1.3808708919469055 1103 44-45 -1.4793511291796264 1103 46-47 -1.1262027779234671 1103 48-49 -0.6814990115266895 1103 50-51 -0.3640201034658723 1103 52-53 -0.31983549142703893 1103 54-55 -0.42901825600105425 1103 56-57 -0.2430203846337733 1103 58-59 -0.5012630755134957 1103 60-61 -0.4978203962875867 1103 62-63 -0.6081854534043813 1103 64-65 -0.5647224075158839 1103 66-67 -0.5364559588939244 1103 68-69 -1.3426834492867137 1103 70-71 -1.6787771147654524 1103 72-73 -0.7667890798857009 1103 74-75 -1.2700780716055426 1103 76-77 -1.2158521055934877 1103 78-79 -1.0869676637618468 1103 80-81 -1.2628525703292048 1103 82-83 -0.8605509012034638 1103 84-85 -1.6854444598527785 1103 86-87 -1.9380687583719158 1103 88-89 -1.2809151565285362 1103 90-91 -1.5226940646310894 1103 92-93 -1.5997689040807295 1103 94-95 -1.0903046499926496 1103 96-97 -1.24472631067324 1103 98-99 -0.6459496590732705 1103 100-101 -0.8001832939152891 1104 1 0.18669974235067954 1104 2 0.12454809733984007 1104 3 -0.19087828407987573 1104 4 -0.11610944389525457 1104 5 -0.12242679644062093 1104 6 0.04353977090429595 1104 7 -0.07336885102718327 1104 8 0.10864758532579089 1104 9 -0.227634964197172 1104 10-11 0.30617316999197186 1104 12-13 0.2191628460529884 1104 14-15 -0.17285124386459216 1104 16-17 -0.26827301075456944 1104 18-19 0.3111962085602187 1104 20-21 0.3178678610939798 1104 22-23 0.18951071106663875 1104 24-25 0.46378273258721237 1104 26-27 0.39613916129977866 1104 28-29 -0.012679047344725802 1104 30-31 -0.3035694579537136 1104 32-33 -0.15501668647778644 1104 34-35 -0.4710743622490483 1104 36-37 -0.492711183770119 1104 38-39 -0.7782188588713623 1104 40-41 -0.5421918938417818 1104 42-43 -0.8732243188668107 1104 44-45 -0.5641735899558498 1104 46-47 -1.4680524889061246 1104 48-49 -1.5485014888181823 1104 50-51 -2.008231499006751 1104 52-53 -2.332750437752388 1104 54-55 -2.503089271690566 1104 56-57 -2.6456958594479794 1104 58-59 -2.129834504084922 1104 60-61 -2.555987200581562 1104 62-63 -2.7256255855265934 1104 64-65 -2.587166668457254 1104 66-67 -2.4628308556734453 1104 68-69 -2.4053424088242856 1104 70-71 -2.1299249264912987 1104 72-73 -1.628671821421623 1104 74-75 -2.3295330674767243 1104 76-77 -1.8793698595158652 1104 78-79 -1.8574053185925656 1104 80-81 -2.070069746216898 1104 82-83 -1.279626045712135 1104 84-85 -1.342686408655421 1104 86-87 -1.0246253231943747 1104 88-89 -0.7343751069827071 1104 90-91 -0.9833381604196916 1104 92-93 -1.5396863276975772 1104 94-95 -1.6388100174575513 1104 96-97 -1.1630417524568877 1104 98-99 -1.154719270964268 1104 100-101 -0.5888042683166148 1105 1 0.13467662096339694 1105 2 0.19391225918955257 1105 3 0.13282113788544336 1105 4 0.10354373529549576 1105 5 -0.024160900486862147 1105 6 0.02041838362105608 1105 7 -0.14851335969770219 1105 8 -0.0589824724776804 1105 9 0.44866561383750536 1105 10-11 0.005595135309896193 1105 12-13 0.3521108229315999 1105 14-15 0.6219464439966771 1105 16-17 0.006293463233866703 1105 18-19 0.08287250913825517 1105 20-21 0.3727811558916656 1105 22-23 0.21263209834987862 1105 24-25 0.34528562276062047 1105 26-27 0.5290871381783901 1105 28-29 0.1607313572795519 1105 30-31 -0.2602168567976406 1105 32-33 0.1340006545626764 1105 34-35 -0.25142118305829797 1105 36-37 0.12867609946687963 1105 38-39 0.1928794070245914 1105 40-41 0.26705666107151416 1105 42-43 0.5111687447170041 1105 44-45 0.2797570458822989 1105 46-47 0.046398378145902086 1105 48-49 -0.48780784719968295 1105 50-51 -0.687422250451835 1105 52-53 -0.046623270122328364 1105 54-55 -0.2632048786269827 1105 56-57 -0.9693952814132949 1105 58-59 -0.7020888393450377 1105 60-61 -0.662923616766534 1105 62-63 -0.18516315778093073 1105 64-65 -0.06115510776361077 1105 66-67 0.040059317736961475 1105 68-69 -0.3301979001537667 1105 70-71 -0.36980931955488217 1105 72-73 -0.7095966769129518 1105 74-75 -0.48560243163857564 1105 76-77 -0.6712773739667313 1105 78-79 -0.672434220326668 1105 80-81 -0.8157344861012952 1105 82-83 -0.5744237335734077 1105 84-85 -1.5276575069213187 1105 86-87 -0.6720241671250129 1105 88-89 -0.24593580062432352 1105 90-91 -0.6740896055063956 1105 92-93 -0.8113626282756101 1105 94-95 -0.818000768902639 1105 96-97 -0.5069723882950399 1105 98-99 0.44065645157908406 1105 100-101 1.4400974657874315 1106 1 -0.17962530463680082 1106 2 0.11440589371559895 1106 3 -0.07303722588632056 1106 4 0.1675885595800466 1106 5 0.19357731718042714 1106 6 0.1177267988130879 1106 7 0.0468127156950473 1106 8 0.2834764799674403 1106 9 0.21727442975296185 1106 10-11 0.3239220263343938 1106 12-13 0.34567442447775676 1106 14-15 0.4519404154141071 1106 16-17 0.16975670661129527 1106 18-19 0.23729287865489823 1106 20-21 0.2728130719170636 1106 22-23 0.15828619955914291 1106 24-25 0.0878296846067883 1106 26-27 0.08431959322999205 1106 28-29 -0.015994767760346917 1106 30-31 0.3323396168361725 1106 32-33 -0.11187685882432419 1106 34-35 0.3010984098350633 1106 36-37 0.3706173030556599 1106 38-39 -0.028936969442654004 1106 40-41 0.3250551716569987 1106 42-43 0.4776391869312704 1106 44-45 0.26576718808592403 1106 46-47 0.4944107190090463 1106 48-49 -0.22719576875719127 1106 50-51 -0.09832334623537164 1106 52-53 -0.26710981219828867 1106 54-55 -0.1522434970177038 1106 56-57 0.011721070230382935 1106 58-59 -0.18811671267387808 1106 60-61 -0.806882622405027 1106 62-63 -0.05237476102927019 1106 64-65 -0.5236005845535665 1106 66-67 -0.40195990280277627 1106 68-69 -0.2907816087959141 1106 70-71 -0.5241233023202305 1106 72-73 0.11889014886100924 1106 74-75 0.3574416479387139 1106 76-77 0.7428507866063363 1106 78-79 0.38411033799100025 1106 80-81 0.6457889721773675 1106 82-83 0.5044797736431654 1106 84-85 1.0156773631095355 1106 86-87 0.7898716447832079 1106 88-89 0.7117754657326358 1106 90-91 0.4058423069727688 1106 92-93 0.42701461914451144 1106 94-95 1.3983544209977126 1106 96-97 1.5423201398087905 1106 98-99 1.3595542848355784 1106 100-101 1.867984979529055 1107 1 0.1458932463418705 1107 2 0.11367554022451287 1107 3 0.41093151498426295 1107 4 0.12866071850496752 1107 5 -0.04353194370183644 1107 6 0.2878626445624306 1107 7 0.20219542092437592 1107 8 0.4613175550477777 1107 9 0.6310562001298265 1107 10-11 0.2627001449437998 1107 12-13 -0.0959520727469041 1107 14-15 0.4948304241783461 1107 16-17 0.4858902152294675 1107 18-19 0.049153819350202355 1107 20-21 0.13976092467779466 1107 22-23 0.19668453435318156 1107 24-25 0.31562693847766354 1107 26-27 0.5276076446468778 1107 28-29 0.8443433859060292 1107 30-31 0.3233745277330442 1107 32-33 0.8287088846755282 1107 34-35 0.9526285003989017 1107 36-37 0.9112249571602504 1107 38-39 0.9334863434407765 1107 40-41 1.0913237956710233 1107 42-43 1.6124624413864268 1107 44-45 1.2387441089156042 1107 46-47 0.8753448411241536 1107 48-49 1.4064771789495012 1107 50-51 1.0656227536769798 1107 52-53 1.0859978419063019 1107 54-55 1.180505553522753 1107 56-57 0.7610272344138451 1107 58-59 0.7659988292484101 1107 60-61 0.6943224608552683 1107 62-63 0.4849097846908563 1107 64-65 0.6356347353412595 1107 66-67 0.9074726125346473 1107 68-69 0.8630308364275727 1107 70-71 1.384199075710736 1107 72-73 1.521187110590489 1107 74-75 1.4501600236325523 1107 76-77 1.121885989644607 1107 78-79 0.8802942410000583 1107 80-81 0.7489331439565099 1107 82-83 0.6662348130822515 1107 84-85 0.6570555401472227 1107 86-87 1.6770502892471306 1107 88-89 0.9522991291857466 1107 90-91 1.1265916496546282 1107 92-93 0.9114215721097487 1107 94-95 1.242314397626398 1107 96-97 1.0853927369458063 1107 98-99 1.5798836742600635 1107 100-101 2.2131500394180463 1108 1 -0.03139118094255622 1108 2 0.10067682722579718 1108 3 0.13425841331115862 1108 4 -0.15119771135346127 1108 5 -0.357077632247524 1108 6 -0.05760067590089335 1108 7 -0.12151757778862304 1108 8 -0.3518742081439825 1108 9 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2316 50-51 -2.5651270593372253 2316 52-53 -2.6817502259072157 2316 54-55 -2.0231842993817573 2316 56-57 -1.8278221796973462 2316 58-59 -2.42826382345665 2316 60-61 -2.060632662555353 2316 62-63 -1.9771009358277176 2316 64-65 -2.354969228757465 2316 66-67 -1.8997917853711108 2316 68-69 -1.9907462765118424 2316 70-71 -1.9123448036210995 2316 72-73 -2.1209999998557834 2316 74-75 -1.5643937036175117 2316 76-77 -1.4341318363339504 2316 78-79 -2.184948217239782 2316 80-81 -2.031498793216265 2316 82-83 -2.755838859318647 2316 84-85 -3.299984474811641 2316 86-87 -3.0805070530615204 2316 88-89 -3.872074468420834 2316 90-91 -3.493733705618375 2316 92-93 -3.2942939601764643 2316 94-95 -3.469197694121295 2316 96-97 -3.9046814340838623 2316 98-99 -4.043137392805509 2316 100-101 -4.041275290923519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 15.0 6 50.0 7 50.0 8 124.0 9 262.0 10 481.0 11 853.0 12 1228.0 13 1675.0 14 2127.0 15 3115.0 16 4023.0 17 5200.0 18 4241.0 19 2089.0 20 1510.0 21 1540.0 22 1665.0 23 1790.0 24 1826.0 25 2131.0 26 2599.0 27 3061.0 28 3830.0 29 4589.0 30 5532.0 31 6673.0 32 8532.0 33 10694.0 34 14590.0 35 19991.0 36 26075.0 37 27340.0 38 10067.0 39 238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.44718016043436 20.59667782957466 16.554110434908377 15.402031575082608 2 16.054882593074606 26.154325313400296 34.30273300630694 23.488059087218165 3 25.40376401494928 26.13009432283324 33.92173874354867 14.544402918668803 4 11.891507099199707 18.72320072966314 40.18375053529022 29.20154163584693 5 9.775644590776624 41.77270805988743 33.63384574314253 14.81780160619341 6 23.90647854111863 39.36621637404134 16.46858611081759 20.258718974022436 7 21.448314600492747 39.237190574442884 21.27479714586033 18.039697679204046 8 25.83185491271293 26.329049157717353 19.74595264975613 28.09314327981358 9 23.463230427842877 20.302654483368464 25.911383746086123 30.322731342702536 10-11 25.178105656557793 25.450060897952824 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36-37 20.292313424950084 23.131468741483925 38.56774207900693 18.008475754559054 38-39 18.914040991128786 25.970966934564366 37.115325787702666 17.99966628660419 40-41 18.618574360286306 27.066243249706627 35.546447023753245 18.768735366253818 42-43 17.799998887708625 27.827861786673637 32.599313716221104 21.772825609396637 44-45 20.362610013486645 24.062678141901756 33.298944704753694 22.275767139857905 46-47 22.809759244531698 27.53393879060559 32.25450338970796 17.401798575154746 48-49 21.346321225081127 26.433399978310256 31.95538586885492 20.264892927753696 50-51 17.024690431102325 28.33796331055885 34.58189776231671 20.055448496022112 52-53 20.741243340044267 30.446147514543455 27.912861639767307 20.89974750564497 54-55 19.316330105834524 26.16943534528305 30.2582184429033 24.25601610597912 56-57 22.64464694383191 26.440629892357702 28.499486954176252 22.415236209634138 58-59 25.50539739390131 25.88441068022179 27.53032384363408 21.079868082242825 60-61 21.547253236189707 27.363635099623195 32.25149817160495 18.83761349258214 62-63 20.94305624301342 27.097920571272848 31.289312548315156 20.669710637398573 64-65 20.366667871652343 30.991304637989863 29.37541884838592 19.266608641971875 66-67 22.287044272100594 27.300711590386438 27.340754191264594 23.07148994624837 68-69 20.84823340322231 27.231951682062633 29.540512432636856 22.379302482078206 70-71 23.75033368926855 28.19285015127247 28.296015750133474 19.7608004093255 72-73 22.43887246709694 24.805557023166315 33.07852520876377 19.677045300972978 74-75 18.981952670949084 32.142877005645005 29.111815577987265 19.76335474541865 76-77 20.005505872931124 27.359183128670583 29.403864121729846 23.231446876668446 78-79 19.92475348842383 28.24302454270921 30.76125221763093 21.070969751236035 80-81 21.60928106247254 27.11240385524479 29.725483435016436 21.552831647266235 82-83 19.645290280241813 26.78091752915594 32.07459025966442 21.49920193093783 84-85 18.99001696282083 28.153833319429385 30.10733850560329 22.748811212146492 86-87 19.093037612132875 28.87258146143964 28.707962337814013 23.326418588613475 88-89 18.64865841891144 28.61854271119231 27.07994427342727 25.652854596468984 90-91 19.570265031964563 24.811396569127712 29.54949293275902 26.06884546614871 92-93 21.33319244309239 27.555072326746714 30.406153195891193 20.7055820342697 94-95 19.888882091552702 26.847655011706866 30.40587512304723 22.857587773693197 96-97 15.981958633883734 27.868460421892117 32.69775150298372 23.451829441240427 98-99 19.11528343964985 28.70740619212609 27.784482423015533 24.392827945208527 100-101 18.21083952059175 26.358555101359805 30.045326881899836 25.385278496148604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2045.0 1 1298.0 2 504.0 3 429.5 4 336.5 5 305.0 6 438.0 7 752.5 8 1104.5 9 1198.0 10 899.0 11 575.5 12 466.5 13 431.5 14 431.5 15 467.5 16 501.5 17 517.0 18 552.5 19 606.0 20 691.5 21 757.0 22 820.5 23 880.5 24 992.5 25 1257.0 26 1828.5 27 2553.0 28 2672.0 29 2756.5 30 3153.0 31 3400.0 32 3618.0 33 3824.0 34 4281.0 35 4550.0 36 4560.5 37 4587.0 38 3728.5 39 2554.5 40 2188.5 41 2153.5 42 2230.5 43 2600.5 44 2908.5 45 2921.5 46 3629.5 47 9725.0 48 22464.0 49 21552.0 50 8756.0 51 3125.5 52 1789.0 53 1554.0 54 1437.0 55 1482.5 56 1786.0 57 1991.0 58 2354.5 59 2751.0 60 2883.5 61 2845.5 62 2521.0 63 1627.0 64 915.0 65 729.0 66 512.0 67 382.0 68 299.0 69 217.5 70 176.0 71 172.5 72 169.0 73 185.5 74 153.0 75 129.0 76 100.5 77 41.0 78 17.5 79 9.5 80 6.0 81 6.0 82 4.0 83 3.0 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026138847332447212 2 0.0038930198154708607 3 5.561456879244087E-4 4 0.0 5 0.0027807284396220433 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005283384035281883 14-15 0.002224582751697635 16-17 0.0 18-19 2.7807284396220435E-4 20-21 8.342185318866131E-4 22-23 0.0011122913758488174 24-25 8.342185318866131E-4 26-27 2.7807284396220435E-4 28-29 0.0 30-31 0.0 32-33 2.7807284396220435E-4 34-35 0.0 36-37 0.0011122913758488174 38-39 0.007786039630941721 40-41 0.0011122913758488174 42-43 0.0 44-45 8.342185318866131E-4 46-47 0.0 48-49 2.7807284396220435E-4 50-51 0.0013903642198110217 52-53 0.0016684370637732261 54-55 0.0 56-57 2.7807284396220435E-4 58-59 0.0 60-61 0.0036149469715086565 62-63 0.0 64-65 2.7807284396220435E-4 66-67 2.7807284396220435E-4 68-69 0.0 70-71 5.561456879244087E-4 72-73 2.7807284396220435E-4 74-75 0.002224582751697635 76-77 5.561456879244087E-4 78-79 0.0 80-81 0.0011122913758488174 82-83 0.0 84-85 0.002224582751697635 86-87 0.0 88-89 0.0013903642198110217 90-91 2.7807284396220435E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.002224582751697635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.09880484291665 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92422434367542 20.07630318838323 2 9.996164336856461 5.217758844106802 3 3.8654960790998976 3.026544833684632 4 1.9284861916126832 2.0132473902863595 5 1.2892090010228434 1.6823407059713362 6 0.8353221957040573 1.3080546579982093 7 0.669110126150699 1.2224082220578503 8 0.4922434367541766 1.0277572312843073 9 0.41339924991476307 0.9710303711160175 >10 2.8810092055915444 15.282327358474825 >50 0.40487555404023184 7.559688335956487 >100 0.2365325605182407 11.118464592984779 >500 0.02130923968632799 4.312909809853789 >1k 0.040487555404023184 21.120744790305267 >5k 0.0021309239686327992 4.060419667536108 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7301 4.060419667536108 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3773 2.0983376805387937 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2970 1.6517526931354938 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2671 1.4854651324460957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2545 1.41539077576762 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2344 1.303605492494814 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2292 1.2746859167227447 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2227 1.238536447007658 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1966 1.0933824224593875 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1963 1.0917139853956144 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1952 1.0855963828284458 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1906 1.060013681183923 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1644 0.9143035109477279 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1596 0.8876085179273562 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1567 0.8714802929775485 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1557 0.8659188360983043 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1294 0.7196525201741849 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1279 0.7113103348553187 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1255 0.6979628383451328 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1176 0.6540273289991047 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 962 0.5350121517832812 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 930 0.5172154897697001 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 925 0.514434761330078 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 837 0.4654939407927301 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 745 0.4143285375036844 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 728 0.40487406080896954 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 698 0.3881896901712372 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 677 0.37651063072482466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 650 0.36149469715086563 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 603 0.33535584981841843 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 442 0.24581639406258865 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 0.24025493718334454 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 416 0.231356606176554 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 400 0.2224582751697635 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 329 0.18297193132713047 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 328 0.18241578563920605 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 327 0.18185963995128163 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 322 0.1790789115116596 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 316 0.17574203738411315 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 298 0.1657314150014738 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 291 0.16183839518600293 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 285 0.15850152105845647 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 284 0.15794537537053205 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 276 0.1534962098671368 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 275 0.15294006417921238 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 258 0.14348558748449744 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 258 0.14348558748449744 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 251 0.13959256766902658 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 247 0.13736798491732893 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 244 0.13569954785355573 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.1334749651018581 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 234 0.13013809097431164 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 231 0.1284696539105384 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 231 0.1284696539105384 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 225 0.12513277978299195 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 212 0.11790288583997464 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 211 0.11734674015205022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 208 0.115678303088277 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 208 0.115678303088277 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 207 0.11512215740035259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 207 0.11512215740035259 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 205 0.11400986602450379 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 205 0.11400986602450379 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 205 0.11400986602450379 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 202 0.11234142896073054 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 201 0.11178528327280615 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 197 0.1095607005211085 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.1095607005211085 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 195 0.10844840914525969 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 194 0.10789226345733528 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 192 0.10677997208148647 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 192 0.10677997208148647 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 191 0.10622382639356207 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 189 0.10511153501771324 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 188 0.10455538932978885 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 184 0.10233080657809118 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 181 0.10066236951431796 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 180 0.10010622382639356 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7807284396220435E-4 0.0 16-17 0.0 0.0 0.0 5.561456879244087E-4 0.0 18-19 0.0 0.0 0.0 5.561456879244087E-4 0.0 20-21 0.0 0.0 0.0 0.0013903642198110217 0.0 22-23 0.0 0.0 0.0 0.00444916550339527 0.0 24-25 0.0 0.0 0.0 0.006951821099055108 0.0 26-27 0.0 0.0 0.0 0.011679059446412583 0.0 28-29 0.0 0.0 0.0 0.027251138708296027 0.0 30-31 0.0 0.0 0.0 0.04782852916149914 0.0 32-33 0.0 0.0 0.0 0.07424544933790855 0.0 34-35 0.0 0.0 0.0 0.09760356823073373 0.0 36-37 0.0 0.0 0.0 0.12096168712355888 0.0 38-39 0.0 0.0 0.0 0.16434105078166278 0.0 40-41 0.0 0.0 0.0 0.20966692434750206 0.0 42-43 0.0 0.0 0.0 0.25999810910466103 0.0 44-45 0.0 0.0 0.0 0.29058612194050354 0.0 46-47 0.0 0.0 0.0 0.33758043257011605 0.0 48-49 0.0 0.0 0.0 0.37178339237746716 0.0 50-51 0.0 0.0 0.0 0.40543020649689393 0.0 52-53 0.0 0.0 0.0 0.4741141989555584 0.0 54-55 0.0 0.0 0.0 0.5856214093844023 0.0 56-57 0.0 0.0 0.0 0.7672029764917219 0.0 58-59 0.0 0.0 0.0 0.9512871991947011 0.0 60-61 0.0 0.0 0.0 1.1451039714363573 0.0 62-63 0.0 0.0 0.0 1.2952633071759478 0.0 64-65 0.0 0.0 0.0 1.4732299273117586 0.0 66-67 0.0 0.0 0.0 1.7632599035643377 0.0 68-69 0.0 0.0 0.0 2.158679487678592 0.0 70-71 0.0 0.0 0.0 2.502377522815877 0.0 72-73 0.0 0.0 0.0 2.852471233364292 0.0 74-75 0.0 0.0 0.0 3.2840402871936334 0.0 76-77 0.0 0.0 0.0 3.6202303555439386 0.0 78-79 0.0 0.0 0.0 3.969767920404429 0.0 80-81 0.0 0.0 0.0 4.247562691522671 0.0 82-83 0.0 0.0 0.0 4.551774382817323 0.0 84-85 0.0 0.0 0.0 5.134893136606065 0.0 86-87 0.0 0.0 0.0 5.857604458063834 0.0 88-89 0.0 0.0 0.0 6.674026327936867 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGAT 25 0.0050164415 56.99416 4 ACATGGA 45 9.6082356E-4 42.217896 3 GCTTTGC 155 3.6379788E-11 33.724983 1 CTTTGCG 150 9.440555E-10 31.663424 2 TACACGA 95 3.4770914E-5 29.996927 2 TACCTCC 115 3.8438393E-6 28.910082 2 ATACACG 100 4.9149738E-5 28.512938 1 ACACGAC 100 4.9336635E-5 28.49708 3 CAGCTAA 100 4.9336635E-5 28.49708 9 TTTGCGT 170 3.601599E-9 27.938314 3 TAGCATT 35 0.008376856 27.147627 40-41 AGATGGG 35 0.008382542 27.143848 94-95 CACGACA 105 6.8788184E-5 27.140076 4 CGACAGC 105 6.8788184E-5 27.140076 6 TTGCGTT 175 4.9076334E-9 27.140076 4 ATAAATC 105 6.8788184E-5 27.140076 3 ACGACAG 110 9.439685E-5 25.906435 5 TGGGAAC 95 0.0012232639 24.997438 8 TGGGAAA 95 0.0012232639 24.997438 6 CATGGGT 140 1.7700777E-5 23.747566 6 >>END_MODULE