FastQCFastQC Report
Thu 26 May 2016
SRR936899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312661
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93930.40615550657878524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64650.27954810497517796No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44450.19220283474318112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.185889760756116No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26580.11493253875081563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111300.027.2454531
GTATCAA155850.025.953221
TACATGG116550.025.4891322
ACATGGG118550.023.5745963
GAGTACT75950.022.11486812-13
GGTATCA115050.022.102211
TCAACGC182800.022.022964
CAACGCA182150.022.0223145
ATCAACG184300.021.7663523
AACGCAG186550.021.5788576
TATCAAC186600.021.5244662
CATGGGA75500.021.0265484
AGTACTT81400.020.25479112-13
GTACTTT83200.019.98791114-15
AGAGTAC146200.019.7942810-11
ACGCAGA207400.019.2716167
GTGTAGC12700.019.0868821
CGCAGAG208550.019.0746048
CATGGGG47350.018.5703564
ACCGTAA1801.2286437E-418.4827188
Produced by FastQC (version 0.11.5)