##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936899_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2312661 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67469810750473 33.0 31.0 34.0 30.0 34.0 2 31.76855103277134 33.0 31.0 34.0 30.0 34.0 3 31.778563741075757 33.0 31.0 34.0 30.0 34.0 4 35.356046995214605 37.0 35.0 37.0 33.0 37.0 5 35.39022450761266 37.0 35.0 37.0 33.0 37.0 6 35.442889813941605 37.0 35.0 37.0 33.0 37.0 7 35.38255498752303 37.0 35.0 37.0 33.0 37.0 8 35.351946523939304 37.0 35.0 37.0 33.0 37.0 9 36.95773786127755 39.0 37.0 39.0 33.0 39.0 10-11 36.82290292438019 39.0 37.0 39.0 33.0 39.0 12-13 36.703614796980624 39.0 37.0 39.0 32.0 39.0 14-15 37.825143633243265 40.0 37.5 41.0 32.5 41.0 16-17 37.76248896833561 40.0 37.0 41.0 32.0 41.0 18-19 37.726660976252035 40.0 37.0 41.0 32.0 41.0 20-21 37.66950754996084 40.0 37.0 41.0 32.0 41.0 22-23 37.52316660331972 40.0 37.0 41.0 32.0 41.0 24-25 37.34781232528243 40.0 37.0 41.0 31.5 41.0 26-27 37.31524594395806 39.5 37.0 41.0 31.5 41.0 28-29 37.39441772918728 40.0 37.0 41.0 32.0 41.0 30-31 37.274847459268784 40.0 37.0 41.0 31.0 41.0 32-33 37.10250724165799 39.5 36.0 41.0 31.0 41.0 34-35 36.91870771375484 39.0 36.0 41.0 30.0 41.0 36-37 36.77209262403785 39.0 36.0 41.0 30.0 41.0 38-39 36.61855542165497 39.0 35.5 41.0 30.0 41.0 40-41 36.4238798941998 39.0 35.0 41.0 30.0 41.0 42-43 36.21938948250522 39.0 35.0 41.0 30.0 41.0 44-45 35.97409974916341 39.0 35.0 40.0 29.0 41.0 46-47 35.638651103642076 39.0 35.0 40.0 27.5 41.0 48-49 35.31742006286265 38.5 35.0 40.0 26.5 41.0 50-51 34.670557639014106 37.5 33.5 39.5 25.5 40.5 52-53 34.97089564791381 38.0 34.5 40.0 26.5 40.5 54-55 35.28220867649863 39.0 35.0 40.0 26.5 41.0 56-57 35.173221021152685 39.0 35.0 40.5 26.0 41.0 58-59 34.929332487554376 38.0 34.0 40.0 26.0 41.0 60-61 34.64846015045006 38.0 34.0 40.0 26.0 41.0 62-63 34.34161794573437 38.0 34.0 40.0 25.0 41.0 64-65 34.04508486111885 37.0 34.0 40.0 24.0 41.0 66-67 33.673468787686566 37.0 33.0 39.5 23.0 41.0 68-69 33.23223983108635 36.0 33.0 39.0 22.0 41.0 70-71 32.87780699376173 36.0 32.5 39.0 22.0 40.5 72-73 32.49993038322521 35.0 32.0 38.5 21.0 40.0 74-75 32.07515152458575 35.0 32.0 37.0 20.0 39.5 76-77 31.63558623594206 35.0 31.0 37.0 18.5 39.0 78-79 31.208320199112624 35.0 31.0 36.0 18.0 39.0 80-81 30.780720131484898 35.0 31.0 36.0 14.0 37.5 82-83 30.37337919392423 34.0 30.5 35.5 9.5 37.0 84-85 30.02669133089545 34.0 30.0 35.0 7.0 37.0 86-87 29.708353061689543 34.0 30.0 35.0 4.5 36.0 88-89 29.33594201657744 34.0 29.5 35.0 2.0 36.0 90-91 28.9956009549173 34.0 29.0 35.0 2.0 36.0 92-93 28.651043321956827 34.0 29.0 35.0 2.0 35.0 94-95 28.251498165965526 34.0 29.0 35.0 2.0 35.0 96-97 27.81553219429912 34.0 28.5 35.0 2.0 35.0 98-99 27.2194465596125 34.0 27.0 35.0 2.0 35.0 100-101 25.01402021307922 31.5 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42014828972711626 1101 2 0.2776045740247106 1101 3 0.4780048653873372 1101 4 0.36656419755119174 1101 5 0.20441756697307767 1101 6 0.24290655552790952 1101 7 0.3580626309950503 1101 8 0.31907604665322253 1101 9 0.41610384108457055 1101 10-11 0.46310029212704507 1101 12-13 0.5174085906834591 1101 14-15 0.5995884978141817 1101 16-17 0.6198775466740827 1101 18-19 0.7455738449212674 1101 20-21 0.7855515291028823 1101 22-23 0.7870690234441113 1101 24-25 0.8848111488382884 1101 26-27 0.9001142185880227 1101 28-29 0.8320000595759183 1101 30-31 0.7687820375230459 1101 32-33 0.7739487591263696 1101 34-35 0.7629933456078462 1101 36-37 0.7966615090699278 1101 38-39 0.6944316839111977 1101 40-41 0.7508954935108534 1101 42-43 0.6704864859180333 1101 44-45 0.7535961526694379 1101 46-47 0.7780821400200892 1101 48-49 0.6650716327441444 1101 50-51 0.653678530976002 1101 52-53 0.8444241013543348 1101 54-55 0.8970654940505582 1101 56-57 0.7427967727064626 1101 58-59 0.5897518521224825 1101 60-61 0.728579867111101 1101 62-63 0.7254555071627209 1101 64-65 0.6308812670452042 1101 66-67 0.537931393624028 1101 68-69 0.6797284671904791 1101 70-71 0.6892307890104377 1101 72-73 0.5892596511614485 1101 74-75 0.6960842272823342 1101 76-77 0.7366243576539873 1101 78-79 0.8308944775854243 1101 80-81 0.8674896058721195 1101 82-83 0.6662626973019776 1101 84-85 0.7799338002032776 1101 86-87 0.8262727321000085 1101 88-89 0.7738644766769305 1101 90-91 0.7684033280086062 1101 92-93 0.6185416718352705 1101 94-95 0.7379639510975586 1101 96-97 0.7302041344100232 1101 98-99 0.7827906144391221 1101 100-101 1.1300506241412016 1102 1 0.4727924013339404 1102 2 0.5117593789252162 1102 3 0.4548044900598107 1102 4 0.36314641552441884 1102 5 0.3916670535533484 1102 6 0.37713129173639715 1102 7 0.3869433759847851 1102 8 0.35258907689187424 1102 9 0.3535082387901127 1102 10-11 0.5573442373150712 1102 12-13 0.5385428942030401 1102 14-15 0.711950405611077 1102 16-17 0.6368420853044938 1102 18-19 0.6130478198522269 1102 20-21 0.5026037031698749 1102 22-23 0.6739077742407957 1102 24-25 0.6395368233655816 1102 26-27 0.660389252225869 1102 28-29 0.6861742504972383 1102 30-31 0.6978725764367297 1102 32-33 0.7829318339794256 1102 34-35 0.5608923081459096 1102 36-37 0.602747303277134 1102 38-39 0.5924682960491481 1102 40-41 0.6604796235299659 1102 42-43 0.6770215223885359 1102 44-45 0.7976701382228768 1102 46-47 0.5631349868439699 1102 48-49 0.7555003382182619 1102 50-51 0.5727556147519408 1102 52-53 0.6795167824675659 1102 54-55 0.7249198421845406 1102 56-57 0.7036795005586001 1102 58-59 0.6263099924808202 1102 60-61 0.7237758518147359 1102 62-63 0.8080133996632455 1102 64-65 0.9582390143718769 1102 66-67 0.8165222335078894 1102 68-69 0.5258698523615024 1102 70-71 0.6144188953768435 1102 72-73 0.7232931827810276 1102 74-75 0.6283398217869731 1102 76-77 0.7319425008449798 1102 78-79 0.8593432418372444 1102 80-81 0.8085171534962221 1102 82-83 0.7873961156151275 1102 84-85 0.8577713294150904 1102 86-87 1.015874212165162 1102 88-89 0.9687948994515203 1102 90-91 0.7627601370164676 1102 92-93 0.8254870014861417 1102 94-95 1.0920934390941603 1102 96-97 0.9016487027833655 1102 98-99 1.0463696810774579 1102 100-101 1.567990129133765 1103 1 0.3233213273575899 1103 2 0.36937689089628734 1103 3 0.43703966058653876 1103 4 0.35783638913919447 1103 5 0.3736420364518125 1103 6 0.2826294349983911 1103 7 0.3280921104712462 1103 8 0.32753899382728235 1103 9 0.4693943425719951 1103 10-11 0.43186600512509443 1103 12-13 0.2510233495924652 1103 14-15 0.4523014756785031 1103 16-17 0.6418400086896128 1103 18-19 0.6794579635051079 1103 20-21 0.5797762087889495 1103 22-23 0.4968189439857369 1103 24-25 0.6841590016124357 1103 26-27 0.6008056745848975 1103 28-29 0.5963111849250708 1103 30-31 0.7138197571266502 1103 32-33 0.6299903700880876 1103 34-35 0.6030396500788697 1103 36-37 0.6502199408223319 1103 38-39 0.7141310537937002 1103 40-41 0.817108911128912 1103 42-43 0.8955760762828007 1103 44-45 0.7914238028373504 1103 46-47 0.7335633802769834 1103 48-49 0.7919492046308463 1103 50-51 0.7740907559617511 1103 52-53 0.7521769261204554 1103 54-55 0.8012580372397551 1103 56-57 0.8026240915876244 1103 58-59 0.8302372376679514 1103 60-61 0.8726214982527267 1103 62-63 0.8638108197746561 1103 64-65 0.8895720789668147 1103 66-67 0.9569496497103742 1103 68-69 0.9289078177675449 1103 70-71 0.8694583510594427 1103 72-73 0.8515485819796922 1103 74-75 0.8686572995549824 1103 76-77 0.7584339989393811 1103 78-79 0.8371918688283557 1103 80-81 0.8049136159217092 1103 82-83 0.8497238787344479 1103 84-85 1.0536577996584207 1103 86-87 1.043460208451684 1103 88-89 0.9011864268628358 1103 90-91 0.8258464753336767 1103 92-93 0.9775123117568363 1103 94-95 0.9450581122105497 1103 96-97 0.9125356616419644 1103 98-99 1.0055336603601717 1103 100-101 1.3361061508282823 1104 1 0.5302971579407867 1104 2 0.4892985201561295 1104 3 0.523882312985041 1104 4 0.39273392096630033 1104 5 0.44245229678525533 1104 6 0.43144347832437546 1104 7 0.5114749711411051 1104 8 0.5016964069036476 1104 9 0.5275450259037413 1104 10-11 0.552735871330448 1104 12-13 0.610011919824224 1104 14-15 0.7524627889129647 1104 16-17 0.8336435695270694 1104 18-19 0.8148968499881448 1104 20-21 0.7842531775245121 1104 22-23 0.8294657484100014 1104 24-25 0.6592421757575906 1104 26-27 0.8445480382430546 1104 28-29 0.7380945289602892 1104 30-31 0.8627907869851086 1104 32-33 0.7501370452856335 1104 34-35 0.8020382674487081 1104 36-37 0.8231493171761031 1104 38-39 0.905330886545606 1104 40-41 0.8348755747707202 1104 42-43 0.931659717941514 1104 44-45 0.7783372523330669 1104 46-47 0.8830809912942215 1104 48-49 0.8640065016520424 1104 50-51 0.8762599843806029 1104 52-53 0.9389146081338993 1104 54-55 0.968804881446701 1104 56-57 0.9160942444274127 1104 58-59 1.0069612848267084 1104 60-61 0.9188074147228775 1104 62-63 0.9215177289816836 1104 64-65 0.9384788400704522 1104 66-67 0.8598796118919338 1104 68-69 1.055668986497821 1104 70-71 0.9343765897930751 1104 72-73 0.9448996662780118 1104 74-75 0.7645320572309515 1104 76-77 0.8655993237540898 1104 78-79 0.9274469681146797 1104 80-81 1.1155017066170423 1104 82-83 0.9241590431050888 1104 84-85 1.053788175059168 1104 86-87 0.9980347194290644 1104 88-89 1.1985468706216444 1104 90-91 1.0377232531582976 1104 92-93 0.9070470879112698 1104 94-95 0.8562610732062872 1104 96-97 0.7867398643628825 1104 98-99 0.8896455320263641 1104 100-101 1.0998617128419816 1105 1 0.36483442693378976 1105 2 0.4116961093481457 1105 3 0.33491046660139645 1105 4 0.34443747991338114 1105 5 0.21769761762595863 1105 6 0.2599748942087743 1105 7 0.34624278709102896 1105 8 0.18419892261145066 1105 9 0.37562278105905733 1105 10-11 0.4563997563689455 1105 12-13 0.45743312148682236 1105 14-15 0.4791299208687718 1105 16-17 0.6287499672337304 1105 18-19 0.6096495135301296 1105 20-21 0.6820663376177336 1105 22-23 0.6285069858045276 1105 24-25 0.6719019496613967 1105 26-27 0.6280654944996797 1105 28-29 0.6654628936081508 1105 30-31 0.4811773167881128 1105 32-33 0.4866014694493188 1105 34-35 0.5614924565860662 1105 36-37 0.5389527103925289 1105 38-39 0.536402933709148 1105 40-41 0.7023817260266867 1105 42-43 0.7103308254498089 1105 44-45 0.6684441563277019 1105 46-47 0.661403894713736 1105 48-49 0.7906949530038716 1105 50-51 0.647400940103573 1105 52-53 0.7456623876654049 1105 54-55 0.8527100913693388 1105 56-57 0.8139510730509301 1105 58-59 0.8320924668079712 1105 60-61 0.7238908321038267 1105 62-63 0.6464095158845922 1105 64-65 0.7606332203386899 1105 66-67 0.8581527026761364 1105 68-69 1.0067046457620066 1105 70-71 0.9412089985549272 1105 72-73 0.8697044593664103 1105 74-75 0.907563370236872 1105 76-77 0.8785168182635843 1105 78-79 0.8717258281028819 1105 80-81 0.8789349760493934 1105 82-83 0.9418566207983652 1105 84-85 0.900982929648702 1105 86-87 0.8953316755418079 1105 88-89 0.8385368325804414 1105 90-91 0.7793045230582258 1105 92-93 0.8999784114243319 1105 94-95 0.9982165957593665 1105 96-97 1.0717450063799987 1105 98-99 1.0782782322041449 1105 100-101 1.329938041237721 1106 1 0.5465997351578338 1106 2 0.46475352728360164 1106 3 0.43629078950968747 1106 4 0.3290522811819443 1106 5 0.4180222166634735 1106 6 0.39543562965993573 1106 7 0.5374856164276878 1106 8 0.4089150693933661 1106 9 0.6583923447503821 1106 10-11 0.6164718784377428 1106 12-13 0.5922479118981201 1106 14-15 0.880352854891008 1106 16-17 0.7854345815377144 1106 18-19 0.6583358467627676 1106 20-21 0.7490951271861377 1106 22-23 0.7670802750291941 1106 24-25 0.7631958951620774 1106 26-27 0.7285010904122089 1106 28-29 0.6860706641767607 1106 30-31 0.6843406552061282 1106 32-33 0.7789495641031152 1106 34-35 0.803258237225414 1106 36-37 0.7025007108947818 1106 38-39 0.7951794982636642 1106 40-41 0.7783485923095341 1106 42-43 0.8682127431741335 1106 44-45 1.0540121176194859 1106 46-47 0.8146848396769357 1106 48-49 0.7468873437195 1106 50-51 0.6398668485022441 1106 52-53 0.6959712395263296 1106 54-55 0.6557515549285 1106 56-57 0.8153950477046052 1106 58-59 0.8452073785139618 1106 60-61 0.8692228716822896 1106 62-63 0.8682441365496132 1106 64-65 0.7510398745982414 1106 66-67 0.7973548222461062 1106 68-69 1.0261628724392935 1106 70-71 1.0292882408339423 1106 72-73 0.9376949663053225 1106 74-75 1.0024158668481178 1106 76-77 1.0628147283231684 1106 78-79 1.1364894277582636 1106 80-81 1.2329845489901068 1106 82-83 1.2981235412432177 1106 84-85 1.3390344693288974 1106 86-87 1.3599686969053373 1106 88-89 1.3647438640263125 1106 90-91 1.2673656768041255 1106 92-93 1.4573337725713245 1106 94-95 1.4741640267111045 1106 96-97 1.4557665327704008 1106 98-99 1.516222338844024 1106 100-101 1.541960616887362 1107 1 0.25077888867672016 1107 2 0.3925375052123208 1107 3 0.5006124772600167 1107 4 0.3946797773721542 1107 5 0.22482771635472432 1107 6 0.24679157434577803 1107 7 0.31412155930069474 1107 8 0.34575472971322085 1107 9 0.3795668358816471 1107 10-11 0.4632164215180907 1107 12-13 0.4328713008817502 1107 14-15 0.32374705041669927 1107 16-17 0.4811797987664619 1107 18-19 0.4294412235210743 1107 20-21 0.4535674502788325 1107 22-23 0.6159237423046662 1107 24-25 0.450012895031783 1107 26-27 0.489480078509807 1107 28-29 0.4171406906328414 1107 30-31 0.4925312749127855 1107 32-33 0.5498963177103704 1107 34-35 0.5525670934958455 1107 36-37 0.6696345605087046 1107 38-39 0.6638168687450232 1107 40-41 0.7154304361903598 1107 42-43 0.8266874682745495 1107 44-45 0.8317810829999885 1107 46-47 0.7397240705812536 1107 48-49 0.8731315440605485 1107 50-51 0.8035602752721687 1107 52-53 0.7620689400571479 1107 54-55 0.9627808265649875 1107 56-57 0.9963798142169864 1107 58-59 0.942872502381654 1107 60-61 0.9006684950995947 1107 62-63 0.793002944383332 1107 64-65 0.9085809891524761 1107 66-67 1.0138159397462019 1107 68-69 0.9858201897819043 1107 70-71 1.0849621342958358 1107 72-73 0.9647883670157213 1107 74-75 1.0790053557983228 1107 76-77 0.9622901389021976 1107 78-79 1.0625186370785116 1107 80-81 1.028319612071133 1107 82-83 0.9408843771872206 1107 84-85 1.028202490901645 1107 86-87 1.0845040160494541 1107 88-89 1.1501239081008876 1107 90-91 1.123687092629055 1107 92-93 1.29627320685319 1107 94-95 1.2552754084887034 1107 96-97 1.2600737178326362 1107 98-99 1.3809663607340212 1107 100-101 1.3617817126334337 1108 1 0.07973684577838824 1108 2 0.19529847357541996 1108 3 0.06771601269355543 1108 4 0.11947357601825104 1108 5 0.1252061482035529 1108 6 -0.020039490156605666 1108 7 0.016675886272849993 1108 8 -0.16968458565956013 1108 9 -0.06423867479013268 1108 10-11 0.025219751718360328 1108 12-13 0.004129313956120484 1108 14-15 0.19235164017896977 1108 16-17 0.19149743509873218 1108 18-19 0.1497762149139561 1108 20-21 0.20124053444559564 1108 22-23 0.1405744409074643 1108 24-25 0.3270111032009808 1108 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-3.30500103143644 2316 100-101 -3.1115245616540186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5404.0 3 1606.0 4 1693.0 5 1815.0 6 2068.0 7 2440.0 8 3120.0 9 3902.0 10 4796.0 11 6165.0 12 7895.0 13 9559.0 14 10729.0 15 13373.0 16 16328.0 17 21137.0 18 18185.0 19 12730.0 20 12327.0 21 13163.0 22 14506.0 23 16181.0 24 18933.0 25 21610.0 26 25624.0 27 30386.0 28 36301.0 29 43793.0 30 54480.0 31 67720.0 32 85315.0 33 110540.0 34 150521.0 35 210471.0 36 311522.0 37 455533.0 38 417818.0 39 72943.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47261185518749 19.51817425059231 14.564624461123909 22.444589433096294 2 14.953573597307168 20.98456954568217 40.692494171000995 23.36936268600967 3 18.93485389094805 25.879609637390626 29.956098485471305 25.229437986190018 4 12.264572874656068 17.26399920335457 38.6361343646428 31.835293557346567 5 13.368596701201394 38.09530199308728 34.11310970528972 14.422991600421604 6 30.173022392533543 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21.519182853738556 28-29 21.489053564752634 27.376018574929727 29.528331440993615 21.60659641932402 30-31 21.750309918673793 27.081776349360272 29.463915862190397 21.703997869775538 32-33 21.593447811610954 27.26537461842497 29.39830116912513 21.742876400838945 34-35 22.0478064778342 27.287661893401964 29.12920779004759 21.535323838716245 36-37 22.001535815875144 26.940308677359692 29.215457662315185 21.842697844449972 38-39 21.7490696300065 27.376088387961474 29.1946037242488 21.680238257783223 40-41 21.995914959680725 27.208938713794268 29.180021509093766 21.615124817431237 42-43 21.726896804768856 27.312006985227782 28.867976858404187 22.09311935159917 44-45 21.955368989745562 27.223233355844094 28.727005602886802 22.094392051523542 46-47 22.167014308541606 27.520800115582635 28.37826146763989 21.93392410823587 48-49 22.07221763664585 27.667918615536625 28.20639938812838 22.05346435968915 50-51 21.880235633373363 27.744404529188486 28.252648215394455 22.12271162204369 52-53 22.215593988735368 27.94814116262772 27.747506121142386 22.08875872749453 54-55 22.02287477363595 27.670836177754936 27.960772505972205 22.345516542636908 56-57 22.140894220094818 27.88233823161673 27.800743438454766 22.176024109833687 58-59 22.409632455237265 27.598966715045563 27.824470491217056 22.16693033850012 60-61 22.334124722157625 27.649056011941138 28.21444293617873 21.80237632972251 62-63 22.07020999220745 27.71502967530453 28.249150542514844 21.96560978997318 64-65 21.969501811588323 27.87269933906388 27.987941922755887 22.1698569265919 66-67 22.098530497308232 27.879229222862318 27.627987618135258 22.39425266169419 68-69 22.00025179882572 27.70211074043132 28.021120816278145 22.276516644464817 70-71 22.18377098112083 27.676632415218037 27.916898606802103 22.222697996859022 72-73 22.146749002660727 27.54955124057588 27.97895640079026 22.324743355973137 74-75 21.873015055999616 28.114041243797676 27.675469319970215 22.337474380232493 76-77 22.039216330930756 27.791042923049204 27.77360921278579 22.396131533234254 78-79 22.039066267360482 27.853142264618157 27.701401299901285 22.406390168120076 80-81 22.06237579476212 27.987306300390447 27.580938340561783 22.369379564285648 82-83 22.078628107612296 27.940062995941606 27.738874034076648 22.242434862369443 84-85 22.0581560424849 27.84682537999859 27.590240605557376 22.504777971959136 86-87 21.941725013840845 27.855373287418576 27.70051159742711 22.50239010131347 88-89 22.0501046280888 27.714065607329808 27.534023063622964 22.701806700958425 90-91 22.003619847634095 27.617823530913437 27.80537109928533 22.573185522167133 92-93 22.227787639264044 27.620193535153863 27.792334408528635 22.359684417053458 94-95 22.04283275948324 27.501376201579774 27.79524717099694 22.660543867940042 96-97 21.839901530066612 27.55807808779976 27.950235289993348 22.65178509214028 98-99 21.7088007107015 27.6707533301686 27.6961664503088 22.9242795088211 100-101 21.725464441888324 27.579295933241067 27.818611269726308 22.876628355144305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 814.0 1 567.0 2 387.0 3 445.5 4 421.0 5 448.0 6 676.5 7 1251.5 8 2046.0 9 2441.0 10 2205.0 11 2051.5 12 2210.0 13 2314.5 14 2520.0 15 2671.5 16 2701.0 17 2843.0 18 3036.5 19 3323.5 20 3762.0 21 4334.0 22 5074.0 23 6205.5 24 7559.5 25 9373.5 26 12341.5 27 15867.0 28 18877.5 29 22390.0 30 27438.0 31 32801.0 32 38320.5 33 44126.5 34 51024.0 35 56772.5 36 62681.5 37 69904.0 38 73317.0 39 73644.5 40 74522.0 41 76998.5 42 79368.0 43 82485.0 44 86547.0 45 88814.5 46 88510.5 47 90730.5 48 98374.0 49 97080.0 50 86637.5 51 79221.5 52 74530.5 53 71785.0 54 67708.0 55 61742.0 56 56205.0 57 49932.0 58 44664.5 59 40077.0 60 34616.5 61 28390.0 62 22199.0 63 16934.5 64 12496.0 65 9318.0 66 6715.5 67 5075.0 68 3538.0 69 2114.0 70 1378.5 71 954.5 72 669.5 73 474.0 74 341.5 75 256.5 76 193.5 77 95.5 78 43.5 79 25.0 80 25.0 81 16.5 82 13.0 83 11.5 84 11.0 85 10.5 86 8.5 87 6.0 88 4.0 89 3.0 90 1.0 91 1.0 92 1.5 93 1.5 94 0.5 95 1.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0591958786869325 2 0.09642571911750145 3 0.06827632757243711 4 0.1286829327774369 5 0.10490080474397241 6 0.10299823450129528 7 0.11047879477363955 8 0.09668516051423014 9 0.11925656202962734 10-11 0.10924644813917819 12-13 0.0930529809600283 14-15 0.09450152875843022 16-17 0.11069499593758013 18-19 0.09102069001898679 20-21 0.07525962516771806 22-23 0.07612442982348039 24-25 0.06406040487559568 26-27 0.05621230262455241 28-29 0.0687087299003183 30-31 0.050482971780126874 32-33 0.051412636785071394 34-35 0.0462886691996795 36-37 0.05128291608670704 38-39 0.05253688283756244 40-41 0.0668277797740352 42-43 0.06477386871659963 44-45 0.05076403329324964 46-47 0.03870000834536493 48-49 0.046050847919344856 50-51 0.029381738179525663 52-53 0.04328347302090536 54-55 0.04838582048990319 56-57 0.053574648424477254 58-59 0.06016878392466514 60-61 0.057228448095073166 62-63 0.06399554452641351 64-65 0.04391045639633306 66-67 0.06654671826091244 68-69 0.05586638076224747 70-71 0.027760229449971266 72-73 0.022009278489151674 74-75 0.031370788887779055 76-77 0.0452941438455528 78-79 0.06423336580674815 80-81 0.0416403441749569 82-83 0.06057956613615225 84-85 0.06633051709697184 86-87 0.06773582466258565 88-89 0.09063152792389373 90-91 0.0654657124412095 92-93 0.0762325304054507 94-95 0.08444817463519297 96-97 0.09115041071735114 98-99 0.12239147890676584 100-101 0.118391757373865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2312661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.97059014709691 #Duplication Level Percentage of deduplicated Percentage of total 1 74.72502998803941 40.329539672140015 2 13.967555359994286 15.076744113822762 3 4.842424383996498 7.840455052409497 4 2.2296917367357194 4.813511155109289 5 1.1513131605242575 3.1068525358806744 6 0.7033492185075232 2.2776103441410265 7 0.46586130545951426 1.7599966707642873 8 0.313205469592244 1.3523107224953619 9 0.23627495111676064 1.147670869387324 >10 1.2236811180505223 12.087436299827003 >50 0.09067250499844465 3.36439996140345 >100 0.04836428779861862 4.584289874528305 >500 0.0013687736926754643 0.4758758594188566 >1k 0.0010467092943987684 1.0941972550603314 >5k 1.6103219913827206E-4 0.689109613611864 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9393 0.40615550657878524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6465 0.27954810497517796 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4445 0.19220283474318112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.185889760756116 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2658 0.11493253875081563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.162011639405862E-5 0.0 20-21 0.0 0.0 0.0 1.2972069836435172E-4 0.0 22-23 0.0 0.0 0.0 2.5944139672870345E-4 0.0 24-25 0.0 0.0 0.0 6.702236082158172E-4 0.0 26-27 0.0 0.0 0.0 0.0011242460524910482 0.0 28-29 0.0 0.0 0.0 0.0021403915230118033 0.0 30-31 0.0 0.0 0.0 0.0045402244427523095 0.0 32-33 0.0 0.0 0.0 0.007869722367437338 0.0 34-35 0.0 0.0 0.0 0.011047879477363954 0.0 36-37 0.0 0.0 0.0 0.013166650883981699 2.162011639405862E-5 38-39 0.0 0.0 0.0 0.014982740661082624 4.324023278811724E-5 40-41 0.0 0.0 0.0 0.01798793683985677 4.324023278811724E-5 42-43 0.0 0.0 0.0 0.021555256044876445 4.324023278811724E-5 44-45 0.0 0.0 0.0 0.024949614318743644 4.324023278811724E-5 46-47 0.0 0.0 0.0 0.029511458877890015 4.324023278811724E-5 48-49 0.0 0.0 0.0 0.03483000751082843 4.324023278811724E-5 50-51 0.0 0.0 0.0 0.04248352871432519 4.324023278811724E-5 52-53 0.0 0.0 0.0 0.05949856031644932 4.324023278811724E-5 54-55 0.0 0.0 0.0 0.07828644146288627 4.324023278811724E-5 56-57 0.0 0.0 0.0 0.10537644730464171 4.324023278811724E-5 58-59 0.0 0.0 0.0 0.14392511483524822 4.324023278811724E-5 60-61 0.0 0.0 0.0 0.19553233266786615 4.324023278811724E-5 62-63 0.0 0.0 0.0 0.23451340252635383 4.324023278811724E-5 64-65 0.0 0.0 0.0 0.2744457575061801 4.324023278811724E-5 66-67 0.0 0.0 0.0 0.3501594051181734 4.324023278811724E-5 68-69 0.0 0.0 0.0 0.4512550693767915 4.324023278811724E-5 70-71 0.0 0.0 0.0 0.5230987161542483 4.324023278811724E-5 72-73 0.0 0.0 0.0 0.5867267187019628 4.324023278811724E-5 74-75 0.0 0.0 0.0 0.72585216769773 4.324023278811724E-5 76-77 0.0 0.0 0.0 0.8268181112579838 4.324023278811724E-5 78-79 0.0 0.0 0.0 0.8980347746600128 4.324023278811724E-5 80-81 0.0 0.0 0.0 0.9902661911970669 4.324023278811724E-5 82-83 0.0 0.0 0.0 1.1607840491970074 4.324023278811724E-5 84-85 0.0 0.0 0.0 1.338458165723381 4.324023278811724E-5 86-87 0.0 0.0 0.0 1.584495090287768 4.324023278811724E-5 88-89 0.0 0.0 0.0 1.8893819716767828 4.324023278811724E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11130 0.0 27.245453 1 GTATCAA 15585 0.0 25.95322 1 TACATGG 11655 0.0 25.489132 2 ACATGGG 11855 0.0 23.574596 3 GAGTACT 7595 0.0 22.114868 12-13 GGTATCA 11505 0.0 22.10221 1 TCAACGC 18280 0.0 22.02296 4 CAACGCA 18215 0.0 22.022314 5 ATCAACG 18430 0.0 21.766352 3 AACGCAG 18655 0.0 21.578857 6 TATCAAC 18660 0.0 21.524466 2 CATGGGA 7550 0.0 21.026548 4 AGTACTT 8140 0.0 20.254791 12-13 GTACTTT 8320 0.0 19.987911 14-15 AGAGTAC 14620 0.0 19.79428 10-11 ACGCAGA 20740 0.0 19.271616 7 GTGTAGC 1270 0.0 19.086882 1 CGCAGAG 20855 0.0 19.074604 8 CATGGGG 4735 0.0 18.570356 4 ACCGTAA 180 1.2286437E-4 18.482718 8 >>END_MODULE