FastQCFastQC Report
Thu 26 May 2016
SRR936899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312661
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108610.4696321683117413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81880.35405102606910394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.23622139172148446No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50820.21974686302921181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA165100.026.9037191
GTACATG107700.023.9955651
GGTATCA119300.023.0959221
TACATGG110350.022.7701232
TCAACGC196050.022.5546824
ATCAACG196000.022.5362033
ACATGGG107500.022.4002763
CAACGCA197950.022.3381945
TATCAAC200400.022.1613352
AACGCAG202900.021.8166336
GAGTACT96700.021.51302512-13
TACACCG2257.1664545E-821.109185
TACGCCG1150.00372637620.6502845
AGTACTT101750.020.32860612-13
GTACTTT105600.019.67911514-15
AGAGTAC164450.019.55278610-11
ACGCAGA225800.019.4357767
CGCAGAG226650.019.3209768
CATGGGA73550.018.8561944
CATGGGG42050.018.6368224
Produced by FastQC (version 0.11.5)