##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936899_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2312661 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.090984800625776 31.0 31.0 34.0 31.0 34.0 2 32.07922778133069 33.0 31.0 34.0 30.0 34.0 3 32.2650725722447 33.0 31.0 34.0 30.0 34.0 4 36.00797479613311 37.0 35.0 37.0 35.0 37.0 5 35.9347452998948 37.0 35.0 37.0 35.0 37.0 6 35.97649071783543 37.0 35.0 37.0 35.0 37.0 7 35.908260224909746 37.0 35.0 37.0 35.0 37.0 8 35.88840431001344 37.0 35.0 37.0 35.0 37.0 9 37.5638115573359 39.0 37.0 39.0 35.0 39.0 10-11 37.49495840505807 39.0 37.0 39.0 35.0 39.0 12-13 37.401112182027546 39.0 37.0 39.0 34.0 39.0 14-15 38.5884844341648 40.0 38.0 41.0 34.0 41.0 16-17 38.50779016033911 40.0 38.0 41.0 34.0 41.0 18-19 38.57785490393966 40.0 38.0 41.0 34.0 41.0 20-21 38.66926324264559 40.0 38.0 41.0 34.0 41.0 22-23 38.60177994094249 40.0 38.0 41.0 34.0 41.0 24-25 38.504346724401024 40.0 38.0 41.0 34.0 41.0 26-27 38.35314817000849 40.0 38.0 41.0 34.0 41.0 28-29 38.181706484435026 40.0 38.0 41.0 33.5 41.0 30-31 38.04450306378669 40.0 37.0 41.0 33.0 41.0 32-33 38.01766622950791 40.0 37.0 41.0 33.0 41.0 34-35 37.93146855505411 40.0 37.0 41.0 33.0 41.0 36-37 37.77507187607695 40.0 37.0 41.0 33.0 41.0 38-39 37.621206264126045 40.0 37.0 41.0 33.0 41.0 40-41 37.45003807302497 40.0 37.0 41.0 32.5 41.0 42-43 37.22710289143113 40.0 36.0 41.0 32.0 41.0 44-45 36.901911477730636 40.0 36.0 41.0 31.0 41.0 46-47 36.6165916664829 39.0 36.0 41.0 30.5 41.0 48-49 36.29688678971972 39.0 36.0 41.0 30.0 41.0 50-51 36.05340947073523 39.0 35.0 40.5 29.5 41.0 52-53 35.802554935634745 39.0 35.0 40.0 28.5 41.0 54-55 35.57548966320615 39.0 35.0 40.0 28.0 41.0 56-57 35.31473138518788 38.0 35.0 40.0 27.5 41.0 58-59 34.997685998942345 38.0 34.0 40.0 27.0 41.0 60-61 34.67776838023386 38.0 34.0 40.0 26.0 41.0 62-63 34.32479122534604 37.0 34.0 40.0 26.0 41.0 64-65 33.9373887915263 37.0 33.0 39.5 26.0 41.0 66-67 33.55973248997583 36.0 33.0 39.0 25.0 40.0 68-69 33.131068496420355 36.0 32.0 39.0 23.5 40.0 70-71 32.6548441384189 35.0 32.0 38.0 22.5 40.0 72-73 32.31549133227914 35.0 31.5 37.5 22.0 39.5 74-75 31.88344919553709 35.0 31.0 37.0 21.5 39.0 76-77 30.462061019751708 33.5 29.5 35.5 20.0 38.0 78-79 31.186856828562423 34.0 31.0 36.0 20.0 38.5 80-81 31.01581360173411 34.0 31.0 36.0 20.0 37.5 82-83 30.684243605093872 34.0 31.0 35.0 18.5 37.0 84-85 30.300996990047395 34.0 30.5 35.0 17.5 36.5 86-87 29.950866339684026 34.0 30.0 35.0 12.0 36.0 88-89 29.598059767514563 34.0 30.0 35.0 7.0 36.0 90-91 29.180907188731943 34.0 29.0 35.0 4.0 35.0 92-93 28.736659199078463 34.0 29.0 35.0 2.0 35.0 94-95 28.263337990306404 33.5 29.0 35.0 2.0 35.0 96-97 27.646970092028187 33.0 27.5 35.0 2.0 35.0 98-99 26.917412236380514 33.0 26.5 35.0 2.0 35.0 100-101 23.859033814294442 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3398877537682097 1101 2 0.2295180557673362 1101 3 0.24470798670987648 1101 4 0.1991645304387646 1101 5 0.24302894628039695 1101 6 0.2436737748091673 1101 7 0.25276187369757963 1101 8 0.23042871309566948 1101 9 0.3179192558661512 1101 10-11 0.32372510937567966 1101 12-13 0.3622977034085366 1101 14-15 0.512041704029393 1101 16-17 0.5498321181447707 1101 18-19 0.4142179560165502 1101 20-21 0.4278939114674287 1101 22-23 0.304534623320869 1101 24-25 0.3591322247343811 1101 26-27 0.3818118111882782 1101 28-29 0.5198219015680436 1101 30-31 0.39044547510530236 1101 32-33 0.44494874275741836 1101 34-35 0.4432395655957819 1101 36-37 0.48170478435434916 1101 38-39 0.4020777388634471 1101 40-41 0.3435946213981751 1101 42-43 0.5261513027341067 1101 44-45 0.4645207475998987 1101 46-47 0.32744349046596 1101 48-49 0.11536575313417785 1101 50-51 0.1838909041105623 1101 52-53 0.10885190453380744 1101 54-55 0.23786573206701433 1101 56-57 0.2791431470464687 1101 58-59 0.34096964933981866 1101 60-61 0.24703415864395595 1101 62-63 0.3146863849229007 1101 64-65 0.20307744916340909 1101 66-67 0.14192498200692683 1101 68-69 0.09863592004090549 1101 70-71 -0.019257372828370478 1101 72-73 0.19611831180937855 1101 74-75 0.07255010241512494 1101 76-77 -0.15298467357052914 1101 78-79 0.0416864255043059 1101 80-81 0.18340812467457823 1101 82-83 0.303330281983893 1101 84-85 0.16352170154123513 1101 86-87 0.1390901083722902 1101 88-89 0.1658852309594927 1101 90-91 0.23233292147313378 1101 92-93 0.35069765537664566 1101 94-95 0.42088283728175924 1101 96-97 0.3167318203355656 1101 98-99 0.504996953782527 1101 100-101 0.8613885180366694 1102 1 0.30083244607045145 1102 2 0.15804206256571973 1102 3 0.2799347765972797 1102 4 0.16509293494269883 1102 5 0.2334412754355526 1102 6 0.2376888941349904 1102 7 0.27055275253052713 1102 8 0.24678113147963643 1102 9 0.3200216211377338 1102 10-11 0.4232444422841155 1102 12-13 0.3464086905907422 1102 14-15 0.45726335971949794 1102 16-17 0.5118890723264897 1102 18-19 0.48522080638124976 1102 20-21 0.4348268482282762 1102 22-23 0.4060633748257274 1102 24-25 0.4362850467724755 1102 26-27 0.49996695246722567 1102 28-29 0.27499858877150274 1102 30-31 0.3345314109455373 1102 32-33 0.4169992349321916 1102 34-35 0.3553859256269547 1102 36-37 0.41640664682867623 1102 38-39 0.40723495152543165 1102 40-41 0.5105069328492036 1102 42-43 0.4288879184128831 1102 44-45 0.4827174935810632 1102 46-47 0.48133839875857376 1102 48-49 0.3395026768647895 1102 50-51 0.29197018319844403 1102 52-53 0.47901779221881213 1102 54-55 0.3768103889609975 1102 56-57 0.40277284749261355 1102 58-59 0.49299057561982806 1102 60-61 0.3733336416834021 1102 62-63 0.48386792305759485 1102 64-65 0.35146327523803933 1102 66-67 0.30790428588058916 1102 68-69 0.17683903950837276 1102 70-71 0.22890449601943885 1102 72-73 0.23176867297360815 1102 74-75 0.04159310574349817 1102 76-77 0.10707171407907268 1102 78-79 0.29441330391235354 1102 80-81 0.27581393871041016 1102 82-83 0.33254032376563814 1102 84-85 0.31664736544605887 1102 86-87 0.20702306283726912 1102 88-89 0.44806336990149376 1102 90-91 0.6077927862135937 1102 92-93 0.423233683278351 1102 94-95 0.6388888212772272 1102 96-97 0.690965674170279 1102 98-99 0.708022146757525 1102 100-101 0.8390043030377328 1103 1 0.13221131834642108 1103 2 -0.04235738041288073 1103 3 0.11127089503761312 1103 4 0.20441424834052668 1103 5 0.2518168739892559 1103 6 0.18622915603277335 1103 7 0.2642080833230622 1103 8 0.2967310484150403 1103 9 0.3237167740743132 1103 10-11 0.3817793294139875 1103 12-13 0.355977609773781 1103 14-15 0.42792934232479496 1103 16-17 0.5055193615867211 1103 18-19 0.48097412896505176 1103 20-21 0.43948579477377336 1103 22-23 0.5362976658449767 1103 24-25 0.4554338789819994 1103 26-27 0.5807625402713867 1103 28-29 0.6069711285936492 1103 30-31 0.6302474766154305 1103 32-33 0.6233561195212687 1103 34-35 0.5930661025056736 1103 36-37 0.6757978322093123 1103 38-39 0.7386406976409461 1103 40-41 0.7015707704334986 1103 42-43 0.7865231426872867 1103 44-45 0.7535392223018746 1103 46-47 0.7679161374471377 1103 48-49 0.7311290327721522 1103 50-51 0.6959267453014419 1103 52-53 0.6723420861697633 1103 54-55 0.7489043496763301 1103 56-57 0.7316646680730869 1103 58-59 0.7221938884313204 1103 60-61 0.6327101686061027 1103 62-63 0.7148082631338184 1103 64-65 0.5375517878931788 1103 66-67 0.6755194769291606 1103 68-69 0.7559721138756146 1103 70-71 0.6874876339066702 1103 72-73 0.9186328623608873 1103 74-75 0.7950860941144562 1103 76-77 0.7195796540771155 1103 78-79 0.7868684734618476 1103 80-81 0.8813462850409088 1103 82-83 1.0104158245962829 1103 84-85 0.865742205668866 1103 86-87 0.6617620201322971 1103 88-89 0.9484261667352634 1103 90-91 1.2724396395312887 1103 92-93 1.311154503174766 1103 94-95 1.2413502206713432 1103 96-97 1.2779288081485838 1103 98-99 1.319480785475399 1103 100-101 1.5050736375437381 1104 1 0.28698185189769987 1104 2 0.16400696848164387 1104 3 0.28465409648311635 1104 4 0.21091961708849283 1104 5 0.16409337398599888 1104 6 0.16965712161366753 1104 7 0.217983781731256 1104 8 0.22687453027678117 1104 9 0.3317347654967193 1104 10-11 0.2721627173288539 1104 12-13 0.3095168711483183 1104 14-15 0.4129620165827177 1104 16-17 0.44450175956749405 1104 18-19 0.47890631001723705 1104 20-21 0.3901554572029511 1104 22-23 0.309537511783752 1104 24-25 0.27231674673539175 1104 26-27 0.3110263657115553 1104 28-29 0.4461755667943663 1104 30-31 0.39599140707576197 1104 32-33 0.36720320468339906 1104 34-35 0.44882170740350347 1104 36-37 0.45170711926454743 1104 38-39 0.33998660827578675 1104 40-41 0.34872133085627155 1104 42-43 0.2845175440114929 1104 44-45 0.40141105926254994 1104 46-47 0.4185576380239624 1104 48-49 0.5010787664502416 1104 50-51 0.5232914315255712 1104 52-53 0.5817542520133969 1104 54-55 0.4350935360610819 1104 56-57 0.43630934437260294 1104 58-59 0.4015881424944965 1104 60-61 0.4305274048850052 1104 62-63 0.4614576536240307 1104 64-65 0.5267076559923325 1104 66-67 0.7008390115291832 1104 68-69 0.5124387146079386 1104 70-71 0.5845421836635509 1104 72-73 0.5626800670625229 1104 74-75 0.6648171378949392 1104 76-77 0.5402435041756561 1104 78-79 0.5083498596452749 1104 80-81 0.7748624157900146 1104 82-83 0.6887351995295141 1104 84-85 0.7176238807075102 1104 86-87 0.5714360854593679 1104 88-89 0.7093081094168028 1104 90-91 0.7874311833177039 1104 92-93 0.8885472363830438 1104 94-95 0.9391550703299352 1104 96-97 1.0818810589717103 1104 98-99 1.1400295759324557 1104 100-101 0.9657463095196164 1105 1 0.22456827125174783 1105 2 0.06654147853885206 1105 3 0.13824438383920779 1105 4 0.12207570615017005 1105 5 0.1152591807258645 1105 6 0.10318446526459724 1105 7 0.10317673656712856 1105 8 0.17113079323074487 1105 9 0.25010569667187355 1105 10-11 0.22674677839157198 1105 12-13 0.32819222346659416 1105 14-15 0.4336917920940593 1105 16-17 0.36326913997626065 1105 18-19 0.34970619354239574 1105 20-21 0.3038746410934223 1105 22-23 0.35919826069713423 1105 24-25 0.2808146618014362 1105 26-27 0.25008102021434553 1105 28-29 0.28341465924408027 1105 30-31 0.4687937120113901 1105 32-33 0.32141837154581054 1105 34-35 0.3108389723004237 1105 36-37 0.35485994901391393 1105 38-39 0.36833511213282577 1105 40-41 0.12883168828795277 1105 42-43 0.2542246464023705 1105 44-45 0.3993990651000203 1105 46-47 0.4814454703505362 1105 48-49 0.47954684389647895 1105 50-51 0.6648865456844462 1105 52-53 0.590066242114176 1105 54-55 0.5688511978382067 1105 56-57 0.5923479679481574 1105 58-59 0.5981792893006599 1105 60-61 0.6161087426713792 1105 62-63 0.45328134107482043 1105 64-65 0.44864929996185055 1105 66-67 0.4268179350751211 1105 68-69 0.3916809264018326 1105 70-71 0.3441618936930695 1105 72-73 0.37101984102734065 1105 74-75 0.41810214599268036 1105 76-77 0.5847853097378071 1105 78-79 0.5151185771882574 1105 80-81 0.6258692995903452 1105 82-83 0.8755417351638961 1105 84-85 0.7535414375733751 1105 86-87 0.7593679915373208 1105 88-89 0.817681197321555 1105 90-91 1.0121970179749198 1105 92-93 0.9487012089511957 1105 94-95 1.0340566073287185 1105 96-97 1.5626248579927378 1105 98-99 1.5011583101877122 1105 100-101 1.4374183050818239 1106 1 0.3598394189847127 1106 2 0.21962226859956502 1106 3 0.20530663615787859 1106 4 0.2513301892263087 1106 5 0.18006212760275986 1106 6 0.2639420039145932 1106 7 0.34890956250985283 1106 8 0.28208063391490157 1106 9 0.4680799798749007 1106 10-11 0.40076861972551114 1106 12-13 0.515519600223179 1106 14-15 0.5654039743170713 1106 16-17 0.6318549523503165 1106 18-19 0.5633640927612902 1106 20-21 0.4920213890320966 1106 22-23 0.497002528264872 1106 24-25 0.5121408931581257 1106 26-27 0.5207575810807086 1106 28-29 0.4972900145397503 1106 30-31 0.588821592801942 1106 32-33 0.5531269899341282 1106 34-35 0.4517555864419691 1106 36-37 0.44291598492742423 1106 38-39 0.5251026063136166 1106 40-41 0.518643824471198 1106 42-43 0.6305258086683239 1106 44-45 0.7936063211503566 1106 46-47 0.6718636449129605 1106 48-49 0.5714497492919932 1106 50-51 0.5947159871859711 1106 52-53 0.7045464547507905 1106 54-55 0.8614511437176517 1106 56-57 0.6845766948593734 1106 58-59 0.6449865285263812 1106 60-61 0.7401246995340642 1106 62-63 0.752680337648151 1106 64-65 0.9071670928210054 1106 66-67 0.9300763574318012 1106 68-69 0.8334863029023154 1106 70-71 0.7976540580223173 1106 72-73 0.8905441644063856 1106 74-75 0.9315150108558079 1106 76-77 0.7828971196644119 1106 78-79 0.8255682585358493 1106 80-81 0.8670307823279195 1106 82-83 1.187847096953206 1106 84-85 0.8744511602531659 1106 86-87 0.8009395017454288 1106 88-89 1.0495258423076734 1106 90-91 1.2809503512999676 1106 92-93 1.148216382391844 1106 94-95 1.0750625659774613 1106 96-97 1.260569768567418 1106 98-99 1.4020958266341843 1106 100-101 1.6599742914746045 1107 1 0.2107903081241247 1107 2 0.11130814943958711 1107 3 0.16117824204732756 1107 4 0.10044841445985497 1107 5 0.10189207225461416 1107 6 0.15187758025481912 1107 7 0.06890287767706127 1107 8 0.11349254991261404 1107 9 0.11487437594558259 1107 10-11 0.2504887631661603 1107 12-13 0.15396362041364142 1107 14-15 0.32444407764872807 1107 16-17 0.31504486391790465 1107 18-19 0.24279236960401818 1107 20-21 0.20581423852704717 1107 22-23 0.17856796881235226 1107 24-25 0.2141800504159832 1107 26-27 0.2926702363756064 1107 28-29 0.24060943057994422 1107 30-31 0.26118405803232037 1107 32-33 0.3498684172025719 1107 34-35 0.3946880752958677 1107 36-37 0.4132336277873989 1107 38-39 0.4042813349490686 1107 40-41 0.3728209952006907 1107 42-43 0.4087692272238286 1107 44-45 0.511588521366761 1107 46-47 0.460825456389351 1107 48-49 0.4635605135723253 1107 50-51 0.6031909955420076 1107 52-53 0.6150999928968233 1107 54-55 0.5614494441838858 1107 56-57 0.6649484961952083 1107 58-59 0.7063198694131927 1107 60-61 0.7214057514321581 1107 62-63 0.47934007399381784 1107 64-65 0.39180897273194404 1107 66-67 0.4168568173256375 1107 68-69 0.5995794713431195 1107 70-71 0.5597901453556915 1107 72-73 0.7466276455047378 1107 74-75 0.7992502121655782 1107 76-77 0.7356869043334839 1107 78-79 0.7101684998696456 1107 80-81 0.8359250656628561 1107 82-83 0.7637794328490592 1107 84-85 0.6626779233778137 1107 86-87 0.7133922688696472 1107 88-89 0.7548890043468894 1107 90-91 1.0646138662756925 1107 92-93 1.1356115069959962 1107 94-95 0.9632120003159734 1107 96-97 1.058629750778966 1107 98-99 1.0192843445840971 1107 100-101 1.0206493462845714 1108 1 0.039104051008727936 1108 2 -0.06402583866884726 1108 3 0.008689920630196468 1108 4 0.09359087732952531 1108 5 0.11209353881003636 1108 6 0.12179383240659547 1108 7 0.12127291713957788 1108 8 0.15053627551255744 1108 9 0.15680145652868305 1108 10-11 0.15827530648164156 1108 12-13 0.09108461651667454 1108 14-15 0.1909053350281411 1108 16-17 0.18640038919891566 1108 18-19 0.16559632078454456 1108 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-1.7012825043873754 2316 94-95 -1.4961584372977477 2316 96-97 -1.3644503509772719 2316 98-99 -1.509536766081407 2316 100-101 -1.5012606443577035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 17.0 5 48.0 6 104.0 7 245.0 8 606.0 9 1148.0 10 2036.0 11 3402.0 12 5267.0 13 7103.0 14 9021.0 15 12439.0 16 16414.0 17 22678.0 18 19524.0 19 12089.0 20 10438.0 21 10625.0 22 11373.0 23 12824.0 24 15037.0 25 18335.0 26 22590.0 27 28080.0 28 35698.0 29 44670.0 30 55950.0 31 70761.0 32 90008.0 33 116418.0 34 158522.0 35 223276.0 36 336541.0 37 511791.0 38 395793.0 39 31778.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66372239038215 20.08158170950528 15.182700387452574 22.07199551266 2 15.356781016535203 21.43851393745776 39.98623183360929 23.21847321239775 3 19.24860460329568 25.81769295564654 30.308863947307884 24.624838493749905 4 12.739880803871214 17.75190376099546 38.24031126076088 31.26790417437244 5 13.28717502781476 38.18221835743946 34.210534281233286 14.320072333512496 6 30.295616558955107 37.57576779618949 17.98307402132092 14.145541623534477 7 27.512734254062423 33.65994136621899 21.270009426374827 17.55731495334377 8 26.043289526653496 34.715939776733386 20.206593184215066 19.034177512398056 9 26.435391957576144 16.967640306988358 20.72443821208556 35.87252952334995 10-11 24.34635817314968 27.266137059608468 27.767529829313283 20.619974937928564 12-13 25.854417250216038 24.652199960780813 28.30349770727741 21.18988508172574 14-15 22.725488084937652 25.843454790823213 27.069553211646667 24.361503912592465 16-17 21.54347308144168 29.218009038073454 27.424469042371534 21.814048838113326 18-19 21.393580814481673 27.557216557031055 30.2804215576775 20.768781070809773 20-21 22.38295239223629 26.53631876765865 30.192021468421338 20.888707371683722 22-23 21.986274685308395 26.598796797282436 30.04201653420021 21.372911983208954 24-25 21.84826510829141 26.77017157680417 30.162249557258463 21.219313757645953 26-27 21.83300088254765 26.800433966106883 30.183281062239715 21.183284089105747 28-29 21.429380663836074 27.035673005449734 30.24117125870615 21.29377507200804 30-31 21.725274366464465 26.684813246138066 30.177727063076016 21.412185324321456 32-33 21.590904422888034 26.869199946987006 30.038780346534622 21.501115283590337 34-35 22.027624283113013 26.778115396373696 29.972394743066577 21.221865577446714 36-37 21.998325240970928 26.55383844348164 29.963441130360724 21.484395185186706 38-39 21.730981321252276 26.929218025673684 29.928805287032805 21.41099536604123 40-41 21.97497055206447 26.80996698025907 29.866325399255196 21.34873706842126 42-43 21.75902984074271 26.971646754100348 29.55392392145671 21.715399483700235 44-45 22.029290258863657 26.827819735489133 29.30865770204346 21.834232303603752 46-47 22.178220433323816 27.239862694816374 28.873804757598236 21.708112114261574 48-49 22.14501174840681 27.369744890502428 28.641530466727836 21.843712894362927 50-51 21.975610736914295 27.419824902698497 28.655524792478953 21.949039567908255 52-53 22.27232385603852 27.622093004400828 28.224609833166316 21.880973306394335 54-55 22.162582888128323 27.342455802006825 28.382041031650456 22.112920278214396 56-57 22.265909525770788 27.53882725318061 28.19575832460416 21.999504896444442 58-59 22.48651916245304 27.27709458628244 28.278117445709643 21.958268805554876 60-61 22.432722846450044 27.376131555563244 28.611389172956393 21.57975642503032 62-63 22.203016352158834 27.3812936699326 28.60084119548866 21.814848782419904 64-65 22.11505594754087 27.458908204089884 28.442841190275693 21.98319465809356 66-67 22.201166621985074 27.56793043508341 28.07578718877829 22.155115754153226 68-69 22.16905114930377 27.353879362344934 28.35227471730617 22.124794771045128 70-71 22.261251395850877 27.40280391713862 28.35659841545927 21.979346271551233 72-73 22.327358468741952 27.230304664260057 28.359393866933775 22.082943000064212 74-75 22.040566855836254 27.784644595175713 27.956633327589564 22.21815522139847 76-77 22.16136104192611 27.461623944928736 28.14668517365435 22.230329839490796 78-79 22.12156341656098 27.548469225603668 28.098573370064596 22.231393987770755 80-81 22.1974731275209 27.674842732620046 27.938254107762344 22.18943003209671 82-83 22.194396663181447 27.536925619491644 28.100355354225638 22.168322363101275 84-85 22.121819928387513 27.51301519446524 27.955398838226735 22.409766038920505 86-87 22.02381582978451 27.54350420075226 27.94752621646041 22.485153753002816 88-89 22.11137238080495 27.401932338740437 27.884865681529057 22.601829598925555 90-91 22.009145767342247 27.308034987500296 28.135871570958827 22.54694767419863 92-93 22.21568574036575 27.30309370893529 28.090865889985604 22.390354660713353 94-95 22.013842105217645 27.209020458745663 28.074411477030285 22.702725959006408 96-97 21.915624848656766 27.255187181314383 28.233426618421326 22.595761351607525 98-99 21.757092490306924 27.268454247148615 27.96742806649386 23.007025196050602 100-101 21.436467480758452 27.495737012075168 28.00487408646012 23.06292142070626 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 894.0 1 630.5 2 431.0 3 491.0 4 394.5 5 379.5 6 693.0 7 1552.5 8 2599.0 9 2966.0 10 2528.5 11 2151.0 12 2211.0 13 2335.0 14 2492.5 15 2714.5 16 3039.5 17 3265.5 18 3449.0 19 3719.0 20 4148.5 21 4802.5 22 5590.0 23 6638.5 24 7884.0 25 9680.5 26 12715.0 27 16205.0 28 19285.0 29 22754.5 30 27145.5 31 32171.0 32 38061.0 33 44200.0 34 50817.0 35 56092.5 36 62549.0 37 69731.0 38 73180.5 39 73922.5 40 74629.5 41 77362.0 42 79431.0 43 82171.5 44 86078.5 45 88414.0 46 87986.0 47 90634.5 48 99023.0 49 97260.5 50 86092.0 51 78946.5 52 74357.0 53 71440.5 54 67842.0 55 61589.0 56 55473.5 57 49283.5 58 44375.5 59 39922.5 60 34374.5 61 27963.5 62 22026.5 63 16958.0 64 12402.0 65 9229.5 66 6613.5 67 4986.5 68 3435.5 69 2041.0 70 1337.0 71 878.0 72 608.0 73 467.5 74 331.0 75 249.0 76 184.0 77 88.0 78 46.5 79 28.0 80 17.5 81 11.0 82 3.0 83 2.0 84 1.5 85 0.0 86 0.0 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016344807993908316 2 0.003459218623049379 3 0.0024646932689226827 4 8.648046557623448E-5 5 0.0017296093115246895 6 1.297206983643517E-4 7 4.324023278811724E-5 8 0.0 9 0.0 10-11 1.297206983643517E-4 12-13 7.567040737920516E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002378212803346448 22-23 0.0 24-25 0.003524078972231555 26-27 0.001643128845948455 28-29 0.003113296760744441 30-31 0.016409668343090492 32-33 8.864247721564035E-4 34-35 0.0025079335017107996 36-37 0.0038700008345364926 38-39 0.0025727938508929757 40-41 0.00454022444275231 42-43 0.0022052518721939795 44-45 6.486034918217585E-4 46-47 1.081005819702931E-4 48-49 3.026816295168207E-4 50-51 1.7296093115246896E-4 52-53 0.0012323466344613414 54-55 0.0024214530361345655 56-57 5.837431426395827E-4 58-59 0.0011458661688851068 60-61 0.0011242460524910482 62-63 0.0 64-65 1.5134081475841034E-4 66-67 4.324023278811724E-5 68-69 0.0 70-71 1.5134081475841034E-4 72-73 2.162011639405862E-5 74-75 4.1078221148711374E-4 76-77 9.080448885504619E-4 78-79 3.459218623049379E-4 80-81 0.005123967585391893 82-83 0.0016863690787365722 84-85 0.003264637575502852 86-87 0.0017079891951306309 88-89 9.945253541266965E-4 90-91 2.8106151312276204E-4 92-93 0.0 94-95 3.026816295168207E-4 96-97 0.0015998886131603379 98-99 0.002443073152528624 100-101 9.729052377326378E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2312661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.184948992005864 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33050506611157 38.267486664330605 2 14.844440085532334 15.49312697356784 3 4.972603037516553 7.7848510761088425 4 2.2857172264256693 4.771201474846907 5 1.2458613858609118 3.250760643613072 6 0.760534745255674 2.381308013268931 7 0.5031197030748916 1.8378693229285445 8 0.33772837890381024 1.4099470580998534 9 0.24998562565658025 1.1740938411260982 >10 1.3143824960230164 12.45153506430355 >50 0.1010602917797445 3.6436259843102987 >100 0.05089444124058574 4.83189744388924 >500 0.0019171811113549756 0.6771509533562778 >1k 9.16912705430499E-4 0.7378322394056557 >5k 2.5006710148104517E-4 0.8148691982541062 >10k+ 8.335570049368173E-5 0.47244404859007355 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10861 0.4696321683117413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8188 0.35405102606910394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5463 0.23622139172148446 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5082 0.21974686302921181 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.648046557623448E-5 0.0 22-23 0.0 0.0 0.0 2.1620116394058622E-4 0.0 24-25 0.0 2.162011639405862E-5 0.0 6.269833754277E-4 0.0 26-27 0.0 4.324023278811724E-5 0.0 0.0010810058197029309 0.0 28-29 0.0 4.324023278811724E-5 0.0 0.0021620116394058617 0.0 30-31 0.0 4.324023278811724E-5 0.0 0.004648325024722603 0.0 32-33 0.0 4.324023278811724E-5 0.0 0.008215644229742275 0.0 34-35 0.0 4.324023278811724E-5 0.0 0.011372181223274833 0.0 36-37 0.0 4.324023278811724E-5 0.0 0.013469332513498519 0.0 38-39 0.0 4.324023278811724E-5 0.0 0.01532866252338756 0.0 40-41 0.0 4.324023278811724E-5 0.0 0.01846357940052606 0.0 42-43 0.0 4.324023278811724E-5 0.0 0.02211737907112197 0.0 44-45 0.0 4.324023278811724E-5 0.0 0.025576597694171345 0.0 46-47 0.0 4.324023278811724E-5 0.0 0.030246542835288007 0.0 48-49 0.0 4.324023278811724E-5 0.0 0.035586711584620484 0.0 50-51 0.0 4.324023278811724E-5 0.0 0.043261852904511296 0.0 52-53 0.0 4.324023278811724E-5 0.0 0.06029850462302949 0.0 54-55 0.0 4.324023278811724E-5 0.0 0.07936744728258918 0.0 56-57 0.0 4.324023278811724E-5 0.0 0.10652231347352681 0.0 58-59 0.0 4.324023278811724E-5 0.0 0.14494126030576898 0.0 60-61 0.0 4.324023278811724E-5 0.0 0.19672143906953937 0.0 62-63 0.0 4.324023278811724E-5 0.0 0.23587546985917954 0.0 64-65 0.0 4.324023278811724E-5 0.0 0.2764348082144335 0.0 66-67 0.0 4.324023278811724E-5 0.0 0.3524511374559436 0.0 68-69 0.0 4.324023278811724E-5 0.0 0.4542818856719597 0.0 70-71 0.0 4.324023278811724E-5 0.0 0.5260822922166284 0.0 72-73 0.0 4.324023278811724E-5 0.0 0.5896886746479488 0.0 74-75 0.0 4.324023278811724E-5 0.0 0.7290951851568388 0.0 76-77 0.0 4.324023278811724E-5 0.0 0.8305583913941559 0.0 78-79 0.0 4.324023278811724E-5 0.0 0.9019263956109433 0.0 80-81 0.0 4.324023278811724E-5 0.0 0.9944821138939084 0.0 82-83 0.0 4.324023278811724E-5 0.0 1.1653026535233657 0.0 84-85 0.0 4.324023278811724E-5 0.0 1.3438415747055017 0.0 86-87 0.0 4.324023278811724E-5 0.0 1.5899001193862827 0.0 88-89 0.0 6.486034918217586E-5 0.0 1.8945491794949627 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16510 0.0 26.903719 1 GTACATG 10770 0.0 23.995565 1 GGTATCA 11930 0.0 23.095922 1 TACATGG 11035 0.0 22.770123 2 TCAACGC 19605 0.0 22.554682 4 ATCAACG 19600 0.0 22.536203 3 ACATGGG 10750 0.0 22.400276 3 CAACGCA 19795 0.0 22.338194 5 TATCAAC 20040 0.0 22.161335 2 AACGCAG 20290 0.0 21.816633 6 GAGTACT 9670 0.0 21.513025 12-13 TACACCG 225 7.1664545E-8 21.10918 5 TACGCCG 115 0.003726376 20.650284 5 AGTACTT 10175 0.0 20.328606 12-13 GTACTTT 10560 0.0 19.679115 14-15 AGAGTAC 16445 0.0 19.552786 10-11 ACGCAGA 22580 0.0 19.435776 7 CGCAGAG 22665 0.0 19.320976 8 CATGGGA 7355 0.0 18.856194 4 CATGGGG 4205 0.0 18.636822 4 >>END_MODULE