FastQCFastQC Report
Thu 26 May 2016
SRR936898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236654
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92410.4131618033008234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63090.2820731324558917No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44380.19842139195423164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.1801798579485249No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24420.10918094618121534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155450.027.666391
GTACATG105950.025.835461
TACATGG111100.024.082342
GAGTACT77950.023.92597412-13
GGTATCA112800.023.592481
ATCAACG180800.023.4429633
TCAACGC181000.023.3393484
CAACGCA180900.023.3038855
TATCAAC184000.023.1674922
AACGCAG184850.022.85586
ACATGGG111050.022.7207183
GTACTTT85600.021.78943414-15
AGTACTT82650.020.86939412-13
ACGCAGA203800.020.5207217
CGCAGAG204350.020.3496638
AGAGTAC144750.020.30514310-11
CATGGGA72950.019.4765224
GCAGAGT209800.019.1188079
GTGTAGC11950.019.0883331
ACTTTTT104950.018.1338716-17
Produced by FastQC (version 0.11.5)