##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936898_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236654 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.742574399080056 33.0 31.0 34.0 30.0 34.0 2 31.84979572164492 33.0 31.0 34.0 30.0 34.0 3 31.865718613607648 33.0 31.0 34.0 30.0 34.0 4 35.45978859492796 37.0 35.0 37.0 33.0 37.0 5 35.47496125909506 37.0 35.0 37.0 33.0 37.0 6 35.550981957870995 37.0 35.0 37.0 33.0 37.0 7 35.48711110435499 37.0 35.0 37.0 33.0 37.0 8 35.434481596169995 37.0 35.0 37.0 33.0 37.0 9 37.068542564026444 39.0 37.0 39.0 33.0 39.0 10-11 36.87401001674823 39.0 37.0 39.0 33.0 39.0 12-13 36.802422949638164 39.0 37.0 39.0 33.0 39.0 14-15 37.94624067915735 40.0 38.0 41.0 33.0 41.0 16-17 37.88710636513292 40.0 38.0 41.0 33.0 41.0 18-19 37.86513761180764 40.0 38.0 41.0 33.0 41.0 20-21 37.79129114293047 40.0 37.5 41.0 32.0 41.0 22-23 37.68593689502266 40.0 37.0 41.0 32.0 41.0 24-25 37.52101464956135 40.0 37.0 41.0 32.0 41.0 26-27 37.62538483824498 40.0 37.0 41.0 32.0 41.0 28-29 37.58602291637419 40.0 37.0 41.0 32.0 41.0 30-31 37.44951141303036 40.0 37.0 41.0 32.0 41.0 32-33 37.26041198146875 40.0 36.5 41.0 31.0 41.0 34-35 37.102999391054674 39.5 36.0 41.0 31.0 41.0 36-37 36.931566974596876 39.0 36.0 41.0 30.5 41.0 38-39 36.77499358416635 39.0 36.0 41.0 30.0 41.0 40-41 36.622533257267335 39.0 35.5 41.0 30.0 41.0 42-43 36.41198236294036 39.0 35.0 41.0 30.0 41.0 44-45 36.16116663551895 39.0 35.0 40.5 29.5 41.0 46-47 35.8119613046989 39.0 35.0 40.0 28.5 41.0 48-49 35.49779089658034 39.0 35.0 40.0 27.0 41.0 50-51 34.84248457740894 38.0 34.0 39.5 26.5 40.5 52-53 35.08231916961675 38.5 34.5 40.0 27.0 40.5 54-55 35.47300901256967 39.0 35.0 40.5 27.0 41.0 56-57 35.35753272522258 39.0 35.0 41.0 27.0 41.0 58-59 35.12914000109092 38.5 35.0 40.0 26.0 41.0 60-61 34.85127963466857 38.0 34.0 40.0 26.0 41.0 62-63 34.538598281182516 38.0 34.0 40.0 26.0 41.0 64-65 34.237744863532754 37.0 34.0 40.0 25.5 41.0 66-67 33.86675878343275 37.0 33.5 39.5 24.5 41.0 68-69 33.48176606663347 36.0 33.0 39.0 23.0 41.0 70-71 33.07338193569501 36.0 33.0 39.0 22.5 40.5 72-73 32.686098520379105 35.0 32.0 38.5 22.0 40.0 74-75 32.25797821209718 35.0 32.0 37.5 20.5 39.5 76-77 31.82834470597598 35.0 32.0 37.0 20.0 39.0 78-79 31.382386815305363 35.0 31.0 36.5 18.0 39.0 80-81 30.945880542989663 35.0 31.0 36.0 17.5 37.5 82-83 30.534157272425688 34.5 31.0 35.5 12.5 37.0 84-85 30.186074377172332 34.0 30.0 35.0 8.5 37.0 86-87 29.870088310485215 34.0 30.0 35.0 7.0 36.0 88-89 29.524370108206277 34.0 30.0 35.0 2.0 36.0 90-91 29.17209814302972 34.0 29.0 35.0 2.0 36.0 92-93 28.8114878295883 34.0 29.0 35.0 2.0 35.0 94-95 28.390358320956217 34.0 29.0 35.0 2.0 35.0 96-97 27.921917739623563 34.0 29.0 35.0 2.0 35.0 98-99 27.337250196051784 34.0 27.0 35.0 2.0 35.0 100-101 25.07996654824573 31.5 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3796024336323711 1101 2 0.4355775038364058 1101 3 0.41467045691407023 1101 4 0.30703278465157524 1101 5 0.363663176490185 1101 6 0.1767752240550564 1101 7 0.2921762843720188 1101 8 0.26812377617715555 1101 9 0.21551769550038102 1101 10-11 0.36008366225996014 1101 12-13 0.3302650893739596 1101 14-15 0.6566044940170244 1101 16-17 0.6734363302806159 1101 18-19 0.6396343639396633 1101 20-21 0.739429915265255 1101 22-23 0.6616586598584817 1101 24-25 0.6153538170086605 1101 26-27 0.645227827636468 1101 28-29 0.7127000223210445 1101 30-31 0.5991545737047517 1101 32-33 0.7224595288455831 1101 34-35 0.6420008459530848 1101 36-37 0.5530697235251196 1101 38-39 0.559487437852745 1101 40-41 0.541414664180401 1101 42-43 0.6048372817962147 1101 44-45 0.6561862493398962 1101 46-47 0.5484927936064068 1101 48-49 0.7494647286062488 1101 50-51 0.7704148804756059 1101 52-53 0.7476508883520765 1101 54-55 0.7811264098787021 1101 56-57 0.8040088376128196 1101 58-59 0.7130516411451211 1101 60-61 0.8772040172358118 1101 62-63 0.8774767983425775 1101 64-65 0.9473236920244048 1101 66-67 0.8329509381148057 1101 68-69 0.8583640458330919 1101 70-71 0.850740369989829 1101 72-73 0.8298066037733562 1101 74-75 0.786993424586683 1101 76-77 0.9543676969250185 1101 78-79 0.8955186984506014 1101 80-81 0.7045487662631054 1101 82-83 0.7196373429700493 1101 84-85 0.7193317166569564 1101 86-87 0.7057159153459089 1101 88-89 0.7763528844099277 1101 90-91 0.9048758401426014 1101 92-93 1.0115479815986177 1101 94-95 0.9211738995781111 1101 96-97 0.8461617257398366 1101 98-99 0.9719726449645982 1101 100-101 1.120971259837976 1102 1 0.3475824177953726 1102 2 0.35491049503289673 1102 3 0.4005558565986611 1102 4 0.28336046413201643 1102 5 0.2842330173216965 1102 6 0.2643079128181043 1102 7 0.39562062364311856 1102 8 0.34274149232224715 1102 9 0.4998425602537466 1102 10-11 0.6157154037974735 1102 12-13 0.593580769666211 1102 14-15 0.5750460302058968 1102 16-17 0.7209917223461417 1102 18-19 0.674048956436387 1102 20-21 0.6348715171643633 1102 22-23 0.5582735845661944 1102 24-25 0.5150527559962939 1102 26-27 0.49521406175787774 1102 28-29 0.4671779877657798 1102 30-31 0.4582811848531989 1102 32-33 0.4888238729742511 1102 34-35 0.3643152103770646 1102 36-37 0.21212249383540183 1102 38-39 0.36648378968697415 1102 40-41 0.2162221914127187 1102 42-43 0.2207467073057643 1102 44-45 0.181828837225261 1102 46-47 0.09651190031317469 1102 48-49 0.19471510540039105 1102 50-51 0.15959945387476893 1102 52-53 0.1518108453659366 1102 54-55 0.18997945018319484 1102 56-57 0.16324295569827285 1102 58-59 0.19915195073182446 1102 60-61 0.017423942742034626 1102 62-63 0.180476158707485 1102 64-65 0.09581302201289077 1102 66-67 -0.005270980324645791 1102 68-69 0.028884188099915775 1102 70-71 0.17128800242506514 1102 72-73 0.24974897423270193 1102 74-75 0.044017869626308936 1102 76-77 0.13781819964991016 1102 78-79 0.21188874023898663 1102 80-81 0.28581049429259764 1102 82-83 0.1702306273839156 1102 84-85 0.2261687003741244 1102 86-87 0.210488225218743 1102 88-89 0.22141351249997143 1102 90-91 0.2827029398757652 1102 92-93 0.46696888893221455 1102 94-95 0.4306642459958212 1102 96-97 0.6040564155209793 1102 98-99 0.7623879752458684 1102 100-101 0.9235568120701281 1103 1 0.42988570573847795 1103 2 0.429576727228941 1103 3 0.4821707949279279 1103 4 0.2290521619146233 1103 5 0.3336312630349241 1103 6 0.34286369464950184 1103 7 0.22566072089466616 1103 8 0.4648981346103582 1103 9 0.3538436859634473 1103 10-11 0.5909337341497363 1103 12-13 0.4460469978826751 1103 14-15 0.6095236971617055 1103 16-17 0.5499671827021118 1103 18-19 0.48874116230533104 1103 20-21 0.6328742394353881 1103 22-23 0.7229756113361816 1103 24-25 0.6422665220429238 1103 26-27 0.6284237981814442 1103 28-29 0.6222863634281524 1103 30-31 0.6316849042658177 1103 32-33 0.7487751447563085 1103 34-35 0.8188175468058674 1103 36-37 0.6763189559863179 1103 38-39 0.6603035764483209 1103 40-41 0.6502007162444272 1103 42-43 0.6914845593830918 1103 44-45 0.8337191685925092 1103 46-47 0.746538723095064 1103 48-49 0.8117218326683542 1103 50-51 0.7877400489878781 1103 52-53 0.8262502413440771 1103 54-55 0.7190042022029743 1103 56-57 0.9267148776732341 1103 58-59 0.9243289584362557 1103 60-61 0.8762201701763672 1103 62-63 0.832435935764174 1103 64-65 0.8453531536425132 1103 66-67 0.8458118503565117 1103 68-69 0.8197888319774904 1103 70-71 0.820736899164551 1103 72-73 0.7599509892806466 1103 74-75 0.6302099191685713 1103 76-77 0.7839831525676999 1103 78-79 0.7890610260305522 1103 80-81 0.8027786865907665 1103 82-83 0.6885576895909011 1103 84-85 0.5274723683476843 1103 86-87 0.5818672235420017 1103 88-89 0.6958695163408599 1103 90-91 0.9301282581783603 1103 92-93 1.095706322956346 1103 94-95 1.004729896108934 1103 96-97 1.0045796518516568 1103 98-99 1.1120177656932562 1103 100-101 1.1336108142202868 1104 1 0.4658446098480624 1104 2 0.33619036694023663 1104 3 0.48519652282108794 1104 4 0.36812517217632035 1104 5 0.4255262402038724 1104 6 0.4269089639576933 1104 7 0.44297303984198066 1104 8 0.4317010510929009 1104 9 0.33867208484398503 1104 10-11 0.5954508451626523 1104 12-13 0.6116488292765965 1104 14-15 0.7489987057540333 1104 16-17 0.7972084307995218 1104 18-19 0.7952265307935704 1104 20-21 0.733521119191856 1104 22-23 0.5753563006866003 1104 24-25 0.785813681663889 1104 26-27 0.6547684979408572 1104 28-29 0.6844917692316557 1104 30-31 0.6571826457103143 1104 32-33 0.7776238951859256 1104 34-35 0.674543107762311 1104 36-37 0.71812064499305 1104 38-39 0.7479453499053861 1104 40-41 0.7126485485221323 1104 42-43 0.689035745124734 1104 44-45 0.7014187561606349 1104 46-47 0.7131759303420822 1104 48-49 0.722735973189792 1104 50-51 0.6280178267656567 1104 52-53 0.6424510809375334 1104 54-55 0.7320154458814798 1104 56-57 0.6646518590646977 1104 58-59 0.7345611968611578 1104 60-61 0.8076901952168782 1104 62-63 0.7955134631368779 1104 64-65 0.9817717474462171 1104 66-67 0.8539807019301406 1104 68-69 0.8367249295863743 1104 70-71 0.8268159731567906 1104 72-73 1.0031661166926327 1104 74-75 0.9563256946877914 1104 76-77 1.018228500434347 1104 78-79 1.1527590424296363 1104 80-81 1.154551775410912 1104 82-83 0.9866348487208683 1104 84-85 0.9564475487866311 1104 86-87 0.9478821005489237 1104 88-89 0.9253307772040209 1104 90-91 1.0369063796493663 1104 92-93 1.084953634784302 1104 94-95 1.1332803699145977 1104 96-97 1.1759935812472477 1104 98-99 1.2216098754786948 1104 100-101 1.248066796937426 1105 1 0.13632557468386963 1105 2 0.3633045779254189 1105 3 0.33078162405135103 1105 4 0.22801570787330405 1105 5 0.29301388914313975 1105 6 0.368209942355449 1105 7 0.3741170871150601 1105 8 0.3593601618165252 1105 9 0.3706104748468917 1105 10-11 0.4033361498221453 1105 12-13 0.4246840419085842 1105 14-15 0.49083368589546694 1105 16-17 0.30695244901128405 1105 18-19 0.2366522306870138 1105 20-21 0.3573029655543465 1105 22-23 0.4821457514276659 1105 24-25 0.5378939877764495 1105 26-27 0.4278056479941412 1105 28-29 0.44246546873761616 1105 30-31 0.2633714958507767 1105 32-33 0.4268598246637936 1105 34-35 0.3603987214405109 1105 36-37 0.2773936276531259 1105 38-39 0.33088635542703315 1105 40-41 0.32854993377835484 1105 42-43 0.5160409761622233 1105 44-45 0.5423189305285518 1105 46-47 0.512587438624557 1105 48-49 0.542584854328986 1105 50-51 0.4033023855179607 1105 52-53 0.3621510228148921 1105 54-55 0.45228329437513537 1105 56-57 0.5897305668046968 1105 58-59 0.4612637058217288 1105 60-61 0.542018878739114 1105 62-63 0.5963465542925519 1105 64-65 0.743441078067157 1105 66-67 0.514796356294184 1105 68-69 0.5033032014065739 1105 70-71 0.5611603982270239 1105 72-73 0.5601880579887748 1105 74-75 0.4853870140716907 1105 76-77 0.5529591988747313 1105 78-79 0.5006896343118399 1105 80-81 0.5528335249023435 1105 82-83 0.6452890674557281 1105 84-85 0.42430936221295923 1105 86-87 0.49418404765732404 1105 88-89 0.40565989664604274 1105 90-91 0.5449587868953216 1105 92-93 0.5063572965105472 1105 94-95 0.5010574211593912 1105 96-97 0.4178552009314451 1105 98-99 0.44011588148469727 1105 100-101 0.4314824706756788 1106 1 0.2103524874733509 1106 2 0.3169276806958763 1106 3 0.251595704419433 1106 4 0.19469438917813875 1106 5 0.19395497014543395 1106 6 0.2281460481157751 1106 7 0.2584267575200556 1106 8 0.2993523621497829 1106 9 0.36251418441566585 1106 10-11 0.41673069872172874 1106 12-13 0.4817260970813777 1106 14-15 0.4812333991198443 1106 16-17 0.5511348036440111 1106 18-19 0.4336145953253663 1106 20-21 0.45243004497307737 1106 22-23 0.21939536439874274 1106 24-25 0.4976266325331409 1106 26-27 0.30806845343184364 1106 28-29 0.3306771390874559 1106 30-31 0.4279828715465399 1106 32-33 0.35287870537221977 1106 34-35 0.3784321024384738 1106 36-37 0.23483718839795387 1106 38-39 0.22385641337304207 1106 40-41 0.3289924072969157 1106 42-43 0.2131452514037946 1106 44-45 0.2500847199296743 1106 46-47 0.2466752321461101 1106 48-49 0.5252046954206335 1106 50-51 0.4499189846269118 1106 52-53 0.3947334412630781 1106 54-55 0.404103905979305 1106 56-57 0.26115345449343863 1106 58-59 0.40219797196369456 1106 60-61 0.398054855686766 1106 62-63 0.3776545347697535 1106 64-65 0.1905487016505205 1106 66-67 0.33408231256090204 1106 68-69 0.09036117105090824 1106 70-71 0.18853604672204227 1106 72-73 0.40147252128897293 1106 74-75 0.44210083326911587 1106 76-77 0.5954906240471658 1106 78-79 0.3041099654431463 1106 80-81 0.3740823972990093 1106 82-83 0.41930938204228596 1106 84-85 0.5115077652508866 1106 86-87 0.4835741373534894 1106 88-89 0.5355484756491826 1106 90-91 0.6512957206808778 1106 92-93 0.7072977866289989 1106 94-95 0.7035296791629158 1106 96-97 0.8011464830312107 1106 98-99 0.8354386419364488 1106 100-101 0.7765410075684969 1107 1 0.2743991043236633 1107 2 0.3048576628519939 1107 3 0.32504516470043754 1107 4 0.3055877721923608 1107 5 0.26417808259648723 1107 6 0.254099446317241 1107 7 0.257772946072123 1107 8 0.3883456016453408 1107 9 0.36939867785196157 1107 10-11 0.4731582955479112 1107 12-13 0.45314654055180625 1107 14-15 0.5176312235363838 1107 16-17 0.5038163525016941 1107 18-19 0.4815150797667229 1107 20-21 0.6247573390328185 1107 22-23 0.5146607535209782 1107 24-25 0.4459943428647577 1107 26-27 0.5101504713028149 1107 28-29 0.5517268385411569 1107 30-31 0.5130342394894214 1107 32-33 0.6974479687459123 1107 34-35 0.7544645600846636 1107 36-37 0.7270881586729558 1107 38-39 0.42307906448720445 1107 40-41 0.7792829818005558 1107 42-43 0.6977295063447357 1107 44-45 0.6246311911540658 1107 46-47 0.6372362268275609 1107 48-49 0.8285185505036523 1107 50-51 0.7533721626961736 1107 52-53 0.8457816844576058 1107 54-55 0.8786912427710334 1107 56-57 0.8710461767347653 1107 58-59 0.8404949163782476 1107 60-61 0.9010174211713888 1107 62-63 0.9065235557346156 1107 64-65 0.9895405338051688 1107 66-67 0.8837788499754708 1107 68-69 0.7469391351027497 1107 70-71 0.8343996874301638 1107 72-73 0.8697696275101023 1107 74-75 0.8608909603587662 1107 76-77 0.8947561162740527 1107 78-79 0.9367039618924338 1107 80-81 0.7341177351022168 1107 82-83 0.9327931377911227 1107 84-85 0.9114067866597786 1107 86-87 0.8009117305898243 1107 88-89 0.8589072489897802 1107 90-91 1.0196005973805065 1107 92-93 0.8813720272481689 1107 94-95 0.8057278402876413 1107 96-97 0.7557476975588813 1107 98-99 0.8143498831283402 1107 100-101 0.8566146596632898 1108 1 0.21774661836906262 1108 2 0.20999844160041192 1108 3 0.1824559398766148 1108 4 0.1833160339283495 1108 5 0.1214146741476938 1108 6 -0.051822354091946465 1108 7 0.12657872542575888 1108 8 0.11493709380855677 1108 9 0.11360338199074249 1108 10-11 0.07813603547108983 1108 12-13 0.27131802580672115 1108 14-15 0.395319260664067 1108 16-17 0.395722866083581 1108 18-19 0.3846881740059729 1108 20-21 0.4016325438461834 1108 22-23 0.4168636597304811 1108 24-25 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-3.4198166327757207 2316 98-99 -3.481274037604482 2316 100-101 -3.6999585636207364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3838.0 3 1342.0 4 1469.0 5 1668.0 6 1797.0 7 2217.0 8 2658.0 9 3507.0 10 4208.0 11 5548.0 12 7072.0 13 8869.0 14 9981.0 15 12344.0 16 15508.0 17 20561.0 18 17579.0 19 11867.0 20 11610.0 21 12196.0 22 13427.0 23 14969.0 24 17297.0 25 20195.0 26 23736.0 27 28172.0 28 33508.0 29 40688.0 30 50484.0 31 62874.0 32 79611.0 33 104803.0 34 142538.0 35 202157.0 36 303526.0 37 452179.0 38 418983.0 39 71636.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54059495220532 19.531253843858927 14.55184910021832 22.376302103717432 2 14.942196609356786 20.959152509167225 40.7831704151127 23.315480466363283 3 18.967296808031083 25.91314746309666 30.02358558068648 25.09597014818578 4 12.260010099894124 17.25722122301756 38.640235777510696 31.84253289957762 5 13.289093173659552 38.07068105925849 34.23540548956167 14.404820277520283 6 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29.530184086169214 21.470757629416585 28-29 21.477461886827047 27.41843272327662 29.55125001816643 21.552855371729905 30-31 21.75487226656394 27.074127045486712 29.529415368256355 21.64158531969299 32-33 21.594139360544105 27.242704437934712 29.4364647652706 21.72669143625059 34-35 22.013655005643006 27.24191064552074 29.28911069417044 21.45532365466581 36-37 22.01328670140513 26.94149666312247 29.25830821248349 21.78690842298891 38-39 21.688379310660906 27.352588134793297 29.33361026074176 21.625422293804032 40-41 21.996295497251804 27.205224310212422 29.21301114368977 21.585469048846004 42-43 21.71130289681667 27.291703554265457 28.940802781459084 22.056190767458787 44-45 21.9163514588978 27.238958786025343 28.82454328406256 22.0201464710143 46-47 22.11981855279963 27.562385050167144 28.42707829633142 21.89071810070181 48-49 22.057847795878438 27.643197706547536 28.260533267043066 22.03842123053096 50-51 21.864581917427977 27.767878860503213 28.25509189374808 22.11244732832073 52-53 22.183188409685126 27.947628517039284 27.822210597031777 22.046972476243813 54-55 22.05601379687174 27.658667825646816 27.99737440916381 22.287943968317634 56-57 22.09149105014828 27.917777325104414 27.83913588779743 22.15159573694987 58-59 22.352158942564948 27.611052523206066 27.90261087925603 22.13417765497296 60-61 22.270646084028556 27.658149150115385 28.271053479485897 21.800151286370163 62-63 22.090399522424367 27.75058746721698 28.232970158230465 21.926042852128187 64-65 21.937802273670354 27.872158806358836 28.058486378010215 22.131552541960595 66-67 22.05179458800776 27.891067026646322 27.69142365245194 22.36571473289398 68-69 21.99281531926883 27.699728198586094 28.03369398358458 22.273762498560494 70-71 22.089266836361425 27.704867961856245 28.029373411347606 22.176491790434724 72-73 22.143564497011784 27.56045354609303 28.041582138989774 22.254399817905416 74-75 21.856370569350954 28.122119851731476 27.746484793711513 22.27502478520606 76-77 21.990505176402873 27.78601026224469 27.85470250022305 22.368782061129384 78-79 21.96314570437621 27.8707507744975 27.76099162516222 22.40511189596407 80-81 22.041297789982355 28.011811507834462 27.651540393254788 22.2953503089284 82-83 22.038652258255674 27.933473839565735 27.783091174382395 22.244782727796196 84-85 22.012833994545584 27.87839746566731 27.63908208251562 22.469686457271482 86-87 21.96142198812066 27.862834133063576 27.725181697413742 22.450562181402027 88-89 22.016299973168767 27.734929791610767 27.52955907342814 22.719211161792327 90-91 21.99429357310373 27.624275545159183 27.843124014952707 22.538306866784378 92-93 22.218460425174502 27.656803950393773 27.806070765216607 22.31866485921512 94-95 21.98824478011621 27.5738748444287 27.79872931287462 22.639151062580467 96-97 21.89750219549978 27.523907857664078 27.97501681147908 22.60357313535706 98-99 21.675446046237294 27.640943319377136 27.779327306647687 22.904283327737883 100-101 21.728362032441794 27.611832124420022 27.790428878271907 22.869376964866277 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 702.0 1 483.0 2 319.5 3 381.0 4 364.5 5 396.5 6 621.5 7 1189.0 8 1981.5 9 2337.5 10 2120.0 11 1946.0 12 2017.5 13 2176.5 14 2361.5 15 2549.5 16 2656.5 17 2784.0 18 3002.0 19 3245.0 20 3606.0 21 4191.0 22 5083.0 23 6172.5 24 7396.5 25 9212.5 26 12174.0 27 15488.5 28 18345.5 29 21792.0 30 26512.0 31 31647.0 32 37271.0 33 43392.0 34 49868.0 35 54905.0 36 61191.5 37 68166.5 38 70883.0 39 71639.0 40 72705.5 41 74969.0 42 77052.0 43 79603.5 44 83492.5 45 85713.5 46 85463.5 47 87956.5 48 94879.0 49 93257.5 50 83511.5 51 76711.0 52 72099.0 53 69429.0 54 65548.5 55 59491.0 56 53831.5 57 47814.0 58 42906.5 59 38476.0 60 33169.0 61 27073.5 62 21315.5 63 16176.5 64 11894.5 65 9026.5 66 6488.0 67 4790.0 68 3346.5 69 1992.5 70 1274.5 71 905.0 72 620.0 73 445.0 74 333.0 75 250.0 76 162.0 77 74.0 78 45.5 79 29.0 80 21.0 81 16.5 82 10.5 83 8.0 84 11.5 85 8.0 86 5.5 87 8.0 88 7.5 89 3.5 90 2.5 91 2.0 92 1.0 93 1.5 94 1.5 95 1.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04225061185145311 2 0.04345777219006605 3 0.024232626056600617 4 0.0441284168226288 5 0.06393478830431529 6 0.06353240152477764 7 0.06523136792726994 8 0.06657265719239543 9 0.06590201255983269 10-11 0.0743521349301233 12-13 0.07421800600361075 14-15 0.07026120267149054 16-17 0.056557697346125056 18-19 0.05074544386391458 20-21 0.0580331155377631 22-23 0.031028491666569798 24-25 0.017548534552058567 26-27 0.013859989072963454 28-29 0.017280276699033467 30-31 0.013301118545827832 32-33 0.007801832558813299 34-35 0.011535087680079261 36-37 0.00590167276655218 38-39 0.009277250750451344 40-41 0.008338348264863496 42-43 0.005812253482210481 44-45 0.0015201278338088948 46-47 0.003599126194753413 48-49 0.0011624506964420963 50-51 0.007064123462994276 52-53 0.0058793179454667555 54-55 0.00916547664502422 56-57 0.02483620622590709 58-59 0.00999260502518494 60-61 0.0222430469799978 62-63 0.016050761539335098 64-65 0.012071603386129458 66-67 0.018621565964158964 68-69 0.028904783663454427 70-71 0.02155004752634963 72-73 0.020275822724480405 74-75 0.019716952197344782 76-77 0.026535172628399387 78-79 0.03798084102413695 80-81 0.031252039877424045 82-83 0.03091671756114267 84-85 0.021617111989605902 86-87 0.026266914775374288 88-89 0.020298177545565833 90-91 0.018040340615937914 92-93 0.032414490573866146 94-95 0.02418791641442977 96-97 0.0371313578228908 98-99 0.0539868929213012 100-101 0.06487369078990314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236654.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.379383908031286 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02891295007673 40.800260615144836 2 13.898139902440423 15.115445707246728 3 4.746323584570911 7.743064570713744 4 2.1441525970376274 4.663907889268459 5 1.139190998980319 3.0974252339062223 6 0.6845941447284436 2.233668469042702 7 0.4744306351571129 1.8059471952857837 8 0.30637045391189077 1.3328189225082012 9 0.23309898024422604 1.1408201041744233 >10 1.2031808803047315 11.883678472412464 >50 0.09300969633946746 3.4763613344229825 >100 0.045792331366380085 4.3804942942996625 >500 0.001648732259868764 0.5776155093262656 >1k 9.892393559211218E-4 1.0514081610971473 >5k 1.6487322598685364E-4 0.6970835211503641 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9241 0.4131618033008234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6309 0.2820731324558917 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4438 0.19842139195423164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4030 0.1801798579485249 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2442 0.10918094618121534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2354821085424925E-5 0.0 14-15 0.0 0.0 0.0 8.94192843416997E-5 0.0 16-17 0.0 0.0 0.0 1.788385686833994E-4 0.0 18-19 0.0 0.0 0.0 2.4590303193967417E-4 0.0 20-21 0.0 0.0 0.0 4.247416006230736E-4 0.0 22-23 0.0 0.0 0.0 4.918060638793483E-4 0.0 24-25 0.0 0.0 0.0 7.153542747335976E-4 0.0 26-27 0.0 0.0 0.0 0.001184805517527521 0.0 28-29 0.0 0.0 0.0 0.002391965856140467 0.0 30-31 0.0 0.0 0.0 0.005298092597245707 0.0 32-33 0.0 0.0 0.0 0.008628960938974022 0.0 34-35 0.0 0.0 0.0 0.012004538922873184 0.0 36-37 0.0 0.0 0.0 0.014351795136842802 0.0 38-39 0.0 0.0 0.0 0.016229600108018494 0.0 40-41 0.0 0.0 0.0 0.019001597922611184 0.0 42-43 0.0 0.0 0.0 0.0224218855486812 0.0 44-45 0.0 0.0 0.0 0.02604336656452004 0.0 46-47 0.0 0.0 0.0 0.030939072382228094 0.0 48-49 0.0 0.0 0.0 0.036840745148780274 0.0 50-51 0.0 0.0 0.0 0.04580502840403567 0.0 52-53 0.0 0.0 0.0 0.06375594973563188 0.0 54-55 0.0 0.0 0.0 0.08369645014383092 0.0 56-57 0.0 0.0 0.0 0.11206471810123514 0.0 58-59 0.0 0.0 0.0 0.14997849466211582 0.0 60-61 0.0 0.0 0.0 0.20434541954186924 0.0 62-63 0.0 0.0 0.0 0.2418120996810414 0.0 64-65 0.0 0.0 0.0 0.28015061784254514 0.0 66-67 0.0 0.0 0.0 0.3616115858778336 0.0 68-69 0.0 0.0 0.0 0.4660533099889388 0.0 70-71 0.0 0.0 0.0 0.5367392542610525 0.0 72-73 0.0 0.0 0.0 0.6007858166707949 0.0 74-75 0.0 0.0 0.0 0.7447508644609314 0.0 76-77 0.0 0.0 0.0 0.8448110436392934 0.0 78-79 0.0 0.0 0.0 0.9176430507356077 0.0 80-81 0.0 0.0 0.0 1.0101025907449253 0.0 82-83 0.0 0.0 0.0 1.1783673290549186 0.0 84-85 0.0 0.0 0.0 1.3600673148372526 0.0 86-87 0.0 0.0 0.0 1.6038242839527257 0.0 88-89 0.0 0.0 0.0 1.9173059400336396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15545 0.0 27.66639 1 GTACATG 10595 0.0 25.83546 1 TACATGG 11110 0.0 24.08234 2 GAGTACT 7795 0.0 23.925974 12-13 GGTATCA 11280 0.0 23.59248 1 ATCAACG 18080 0.0 23.442963 3 TCAACGC 18100 0.0 23.339348 4 CAACGCA 18090 0.0 23.303885 5 TATCAAC 18400 0.0 23.167492 2 AACGCAG 18485 0.0 22.8558 6 ACATGGG 11105 0.0 22.720718 3 GTACTTT 8560 0.0 21.789434 14-15 AGTACTT 8265 0.0 20.869394 12-13 ACGCAGA 20380 0.0 20.520721 7 CGCAGAG 20435 0.0 20.349663 8 AGAGTAC 14475 0.0 20.305143 10-11 CATGGGA 7295 0.0 19.476522 4 GCAGAGT 20980 0.0 19.118807 9 GTGTAGC 1195 0.0 19.088333 1 ACTTTTT 10495 0.0 18.13387 16-17 >>END_MODULE