FastQCFastQC Report
Thu 26 May 2016
SRR936898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236654
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105930.47360923951581246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.36022558697053725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53550.23942013382490093No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49290.22037382626011892No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22880.10229566128690445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA173550.025.107261
GTACATG106100.024.273841
GGTATCA128600.022.872011
TACATGG111950.022.828472
GAGTACT94100.022.08104912-13
CCCTACG1302.9353448E-421.9312081
ACATGGG109250.021.7824043
GTATACG1100.00287202321.5989151
TCAACGC203350.021.2538784
ATCAACG203550.021.1886983
CAACGCA205450.021.0366335
TATCAAC208400.020.856532
AACGCAG209900.020.65766
GTACTTT99700.020.6037614-15
AGTACTT98150.020.54086112-13
AGAGTAC164700.019.33422510-11
CATGGGG43800.019.0844444
ACGCAGA231550.018.6235587
CGCAGAG231500.018.4019158
TACTTTT114150.017.64190314-15
Produced by FastQC (version 0.11.5)