##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936898_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236654 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15854709758416 33.0 31.0 34.0 31.0 34.0 2 32.22503480645643 33.0 31.0 34.0 30.0 34.0 3 32.36783874483939 34.0 31.0 34.0 31.0 34.0 4 36.053077498799546 37.0 35.0 37.0 35.0 37.0 5 35.96593438234076 37.0 35.0 37.0 35.0 37.0 6 35.99904723752534 37.0 35.0 37.0 35.0 37.0 7 35.948823555185555 37.0 35.0 37.0 35.0 37.0 8 35.93388203986848 37.0 35.0 37.0 35.0 37.0 9 37.619531675440186 39.0 37.0 39.0 35.0 39.0 10-11 37.51759078516391 39.0 37.0 39.0 35.0 39.0 12-13 37.44671974297321 39.0 37.0 39.0 34.5 39.0 14-15 38.65494215913593 40.0 38.0 41.0 34.0 41.0 16-17 38.57986818703295 40.0 38.0 41.0 34.0 41.0 18-19 38.62383542559556 40.0 38.0 41.0 34.0 41.0 20-21 38.7239096883112 40.0 38.0 41.0 34.0 41.0 22-23 38.65974643373539 40.0 38.0 41.0 34.0 41.0 24-25 38.578783084017466 40.0 38.0 41.0 34.0 41.0 26-27 38.413621194874125 40.0 38.0 41.0 34.0 41.0 28-29 38.26392302966842 40.0 38.0 41.0 34.0 41.0 30-31 38.2110292874982 40.0 38.0 41.0 34.0 41.0 32-33 38.12824536115107 40.0 37.0 41.0 34.0 41.0 34-35 38.01281311280154 40.0 37.0 41.0 33.0 41.0 36-37 37.84374203609499 40.0 37.0 41.0 33.0 41.0 38-39 37.70794924024905 40.0 37.0 41.0 33.0 41.0 40-41 37.49146962382201 40.0 37.0 41.0 33.0 41.0 42-43 37.265973413858376 40.0 36.5 41.0 32.0 41.0 44-45 37.02421943671216 40.0 36.0 41.0 31.0 41.0 46-47 36.67325567566553 39.5 36.0 41.0 31.0 41.0 48-49 36.32312977331317 39.0 36.0 41.0 30.0 41.0 50-51 36.09389047210699 39.0 35.0 41.0 29.5 41.0 52-53 35.88860033782606 39.0 35.0 40.0 29.0 41.0 54-55 35.63198957013468 39.0 35.0 40.0 28.0 41.0 56-57 35.39188783781488 38.0 35.0 40.0 28.0 41.0 58-59 35.05920741428938 38.0 34.0 40.0 27.0 41.0 60-61 34.733629788067354 38.0 34.0 40.0 26.0 41.0 62-63 34.415177313969885 37.5 34.0 40.0 26.0 41.0 64-65 34.01843959772052 37.0 33.5 39.5 26.0 41.0 66-67 33.58910117523766 36.0 33.0 39.0 25.0 40.0 68-69 33.16036678002051 36.0 32.5 39.0 24.0 40.0 70-71 32.76601879414518 35.0 32.0 38.0 23.0 40.0 72-73 32.377607801653724 35.0 32.0 37.5 22.5 39.5 74-75 31.90475706121734 35.0 31.0 37.0 21.5 39.0 76-77 30.42321431924652 33.5 29.5 35.5 20.0 38.0 78-79 31.13742715681549 34.0 31.0 36.0 20.0 38.5 80-81 30.859877298858027 34.0 31.0 36.0 18.0 37.5 82-83 30.645482493045414 34.0 31.0 35.0 18.0 37.0 84-85 30.310189908676087 34.0 30.5 35.0 17.5 36.5 86-87 29.953433789938003 34.0 30.0 35.0 12.5 36.0 88-89 29.58748089780538 34.0 30.0 35.0 7.0 36.0 90-91 29.140762049025017 34.0 29.0 35.0 3.5 35.0 92-93 28.71725354033302 34.0 29.0 35.0 2.0 35.0 94-95 28.238871099419043 33.5 29.0 35.0 2.0 35.0 96-97 27.61527755298763 33.0 27.0 35.0 2.0 35.0 98-99 26.853157439639748 33.0 26.5 35.0 2.0 35.0 100-101 23.595934596947046 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32191524501568836 1101 2 0.3092553001504683 1101 3 0.32564019349489115 1101 4 0.18879719822138696 1101 5 0.3019843711616943 1101 6 0.2906588971513884 1101 7 0.2674623636354809 1101 8 0.24126944354524227 1101 9 0.33789833502857647 1101 10-11 0.3782802751277501 1101 12-13 0.359950008852401 1101 14-15 0.4426638564160186 1101 16-17 0.3720313163138158 1101 18-19 0.4224419831188726 1101 20-21 0.4273563653177419 1101 22-23 0.47091966202255264 1101 24-25 0.555064090324791 1101 26-27 0.41545599910141107 1101 28-29 0.49731590143282745 1101 30-31 0.4402458166306005 1101 32-33 0.5065407361354772 1101 34-35 0.5651920414256182 1101 36-37 0.5824251447740281 1101 38-39 0.45477849420534966 1101 40-41 0.5825613178371825 1101 42-43 0.479679871611161 1101 44-45 0.5356950986206357 1101 46-47 0.5772447381843122 1101 48-49 0.5745034062801508 1101 50-51 0.45509661161725745 1101 52-53 0.524556046734169 1101 54-55 0.632251714868751 1101 56-57 0.5251555727302062 1101 58-59 0.6680735084468594 1101 60-61 0.5988884366368765 1101 62-63 0.5606458564000576 1101 64-65 0.5627092679997858 1101 66-67 0.6110634919117359 1101 68-69 0.547039569572533 1101 70-71 0.66816392183798 1101 72-73 0.583070489958196 1101 74-75 0.49764494578339225 1101 76-77 0.48184653134451594 1101 78-79 0.6495896264563932 1101 80-81 0.9319737321581059 1101 82-83 0.6769724853577408 1101 84-85 0.5257662276382682 1101 86-87 0.4772790410587717 1101 88-89 0.34616305200962216 1101 90-91 0.21259013167722784 1101 92-93 0.46284332088475466 1101 94-95 0.5265805008216482 1101 96-97 0.3760955636857872 1101 98-99 0.6293588718272396 1101 100-101 0.7497712564220542 1102 1 0.27466771372430543 1102 2 0.2888869984356788 1102 3 0.2147784994018238 1102 4 0.19242061868039428 1102 5 0.20823769826225913 1102 6 0.16193795105706954 1102 7 0.21760014718763188 1102 8 0.20575035763285143 1102 9 0.2799685235420952 1102 10-11 0.3463710933283224 1102 12-13 0.304252473901208 1102 14-15 0.29827756922735205 1102 16-17 0.26966246230033164 1102 18-19 0.3870283247056392 1102 20-21 0.34011438196655064 1102 22-23 0.3281069894292088 1102 24-25 0.26736970447427666 1102 26-27 0.35278600505692026 1102 28-29 0.41329871030421117 1102 30-31 0.41084869972959126 1102 32-33 0.3735672356596993 1102 34-35 0.32128302681299203 1102 36-37 0.33467114559913824 1102 38-39 0.42188293106030983 1102 40-41 0.522465147995355 1102 42-43 0.40714492041972505 1102 44-45 0.4497274987577171 1102 46-47 0.3692526059453769 1102 48-49 0.48291134291219606 1102 50-51 0.4563476014296768 1102 52-53 0.3298772034339521 1102 54-55 0.4561671008789503 1102 56-57 0.4619114458944793 1102 58-59 0.45109331800362185 1102 60-61 0.35721034692493703 1102 62-63 0.2831166985224769 1102 64-65 0.3430710361208398 1102 66-67 0.2409408133961577 1102 68-69 0.14530725228026142 1102 70-71 0.16781935898572442 1102 72-73 0.28056125309525015 1102 74-75 0.32288826080658595 1102 76-77 0.2132011478629039 1102 78-79 0.2527219460776351 1102 80-81 0.21330483023437807 1102 82-83 0.2730461531866766 1102 84-85 0.2331366079591355 1102 86-87 0.2613443919680698 1102 88-89 0.33163100454520844 1102 90-91 0.3156388578967757 1102 92-93 0.5751209042119854 1102 94-95 0.4706924893631168 1102 96-97 0.5758319117603925 1102 98-99 0.5957889469864419 1102 100-101 1.002026510745619 1103 1 0.24252135333841807 1103 2 0.15488952133355127 1103 3 0.11771529357996968 1103 4 0.16860478589064343 1103 5 0.13513433884426718 1103 6 0.21707369127621234 1103 7 0.15378438532113137 1103 8 0.15154922833758633 1103 9 0.22914196223061367 1103 10-11 0.18271390404458998 1103 12-13 0.3212505130701544 1103 14-15 0.35236334972499606 1103 16-17 0.33923098687396447 1103 18-19 0.4154475694162869 1103 20-21 0.3159333929062669 1103 22-23 0.3432928408698075 1103 24-25 0.3137980387883914 1103 26-27 0.3241845269768362 1103 28-29 0.41327122754201184 1103 30-31 0.29952312237980294 1103 32-33 0.3257028038899463 1103 34-35 0.2638134189063095 1103 36-37 0.33547819316441974 1103 38-39 0.2812585621049166 1103 40-41 0.33564590044777276 1103 42-43 0.2609065816315379 1103 44-45 0.20613250917062942 1103 46-47 0.17477935987260906 1103 48-49 0.15704567037945338 1103 50-51 0.2343982914637195 1103 52-53 0.16124287606519516 1103 54-55 0.1304842527427894 1103 56-57 0.05555487015602267 1103 58-59 0.1875760839476186 1103 60-61 0.19258505517916547 1103 62-63 0.13793777589009437 1103 64-65 0.02572797500607038 1103 66-67 -0.1055772191375226 1103 68-69 0.06964661691426244 1103 70-71 0.07050161747380912 1103 72-73 0.08487216568253331 1103 74-75 -0.029377510703504583 1103 76-77 0.09861063806456016 1103 78-79 0.2887129380359248 1103 80-81 0.5461917082300225 1103 82-83 0.4247538571663938 1103 84-85 0.29218061367318526 1103 86-87 0.28316097693836895 1103 88-89 0.23719511138794047 1103 90-91 0.2538621396774374 1103 92-93 0.42641985381884595 1103 94-95 0.5981782264005595 1103 96-97 0.7538748002361331 1103 98-99 0.7139168527467668 1103 100-101 0.8396993295451729 1104 1 0.17108839806763143 1104 2 0.14355991952670166 1104 3 0.1356609900497503 1104 4 0.17436164748046679 1104 5 0.04600118226745309 1104 6 0.21470466294606894 1104 7 0.17587895005821252 1104 8 0.16160225529295502 1104 9 0.23871013623758586 1104 10-11 0.161131810461832 1104 12-13 0.24209673489958305 1104 14-15 0.30746535296553645 1104 16-17 0.2700596557991801 1104 18-19 0.21435941210298637 1104 20-21 0.3092774052792251 1104 22-23 0.2758215638039587 1104 24-25 0.281398814553107 1104 26-27 0.3751309108330716 1104 28-29 0.33151520063574225 1104 30-31 0.3991266476903519 1104 32-33 0.3470885013417089 1104 34-35 0.39211839754823075 1104 36-37 0.4126373231295517 1104 38-39 0.44361371995830723 1104 40-41 0.41948556755632893 1104 42-43 0.3898467790096518 1104 44-45 0.4430476169430122 1104 46-47 0.3711908343165575 1104 48-49 0.4671244743270151 1104 50-51 0.4957401680287532 1104 52-53 0.5022632767133004 1104 54-55 0.346998712407931 1104 56-57 0.31926884374073694 1104 58-59 0.3110448078637589 1104 60-61 0.40553062890136005 1104 62-63 0.37359472094827595 1104 64-65 0.5642430975081609 1104 66-67 0.42996127450415145 1104 68-69 0.4955070774631878 1104 70-71 0.44896923666171773 1104 72-73 0.5178212990846021 1104 74-75 0.5705574330781076 1104 76-77 0.5901165053989708 1104 78-79 0.7543589585593189 1104 80-81 0.8195536417499341 1104 82-83 0.7493015323271948 1104 84-85 0.6270482917799285 1104 86-87 0.6753210888841608 1104 88-89 0.6272132780300588 1104 90-91 0.6648057003392935 1104 92-93 0.7330647941635213 1104 94-95 1.142029800024563 1104 96-97 1.3114833090985094 1104 98-99 1.3731173977460323 1104 100-101 1.5014622303160259 1105 1 0.19000973876316607 1105 2 0.1320274192845261 1105 3 0.14198667056897563 1105 4 0.14045193131268974 1105 5 0.15956555308989095 1105 6 0.18613254243846455 1105 7 0.18693678208378373 1105 8 0.1647844697654932 1105 9 0.178492660697529 1105 10-11 0.1562628285767218 1105 12-13 0.20235538064210346 1105 14-15 0.3061466955984855 1105 16-17 0.282782459247791 1105 18-19 0.2909541520041756 1105 20-21 0.34277420853408813 1105 22-23 0.14210680065372117 1105 24-25 0.283573569953667 1105 26-27 0.2527900215566987 1105 28-29 0.2685223690945193 1105 30-31 0.24116142440254862 1105 32-33 0.1905896637944764 1105 34-35 0.2649932885682844 1105 36-37 0.17508767670894798 1105 38-39 0.3266770251990323 1105 40-41 0.4473808014999534 1105 42-43 0.48234895860860405 1105 44-45 0.4916841567085797 1105 46-47 0.4409561641625359 1105 48-49 0.39409337871527583 1105 50-51 0.23512552112378415 1105 52-53 0.29304720580368127 1105 54-55 0.20019787931678223 1105 56-57 0.10135859478210563 1105 58-59 0.262496662944649 1105 60-61 0.29494867598069874 1105 62-63 0.3707612423083475 1105 64-65 0.26430727458846803 1105 66-67 0.17296597275078085 1105 68-69 0.2631351894461389 1105 70-71 0.2223078979035975 1105 72-73 0.24329596730120073 1105 74-75 0.20433152975542868 1105 76-77 0.08624892814430041 1105 78-79 0.2629405239957201 1105 80-81 0.5609866069732234 1105 82-83 0.3527368224560341 1105 84-85 0.016668557206646284 1105 86-87 0.0704315218978202 1105 88-89 0.22053401812499374 1105 90-91 0.23281039858592578 1105 92-93 0.07684930448029093 1105 94-95 0.13354147298784724 1105 96-97 0.4569703283758493 1105 98-99 0.6205200423260955 1105 100-101 0.6655618137704487 1106 1 0.16524756951039876 1106 2 0.09320713857530905 1106 3 0.08507195086457386 1106 4 0.08364514639046661 1106 5 0.04183962865211299 1106 6 0.14396628348165308 1106 7 0.06477410782228787 1106 8 0.105686631218596 1106 9 0.15987883480342902 1106 10-11 0.10514517178569349 1106 12-13 0.11532656126741614 1106 14-15 0.21190741915545175 1106 16-17 0.16426406109450653 1106 18-19 0.23774456944391886 1106 20-21 0.16491216415477794 1106 22-23 0.27686268684161064 1106 24-25 0.1140020198289875 1106 26-27 0.16389430467672383 1106 28-29 0.08324325526272247 1106 30-31 0.0324728713982978 1106 32-33 0.1374072618910489 1106 34-35 0.14554267267035215 1106 36-37 0.14967681547604883 1106 38-39 0.12213982512182042 1106 40-41 0.28127655777218763 1106 42-43 0.2641356534801318 1106 44-45 0.2993724418454846 1106 46-47 0.30331272710939317 1106 48-49 0.3138886768555622 1106 50-51 0.12853962827859533 1106 52-53 0.2251273729844243 1106 54-55 0.3628812995100361 1106 56-57 0.2314843151679895 1106 58-59 0.3314401035430947 1106 60-61 0.1941567931490269 1106 62-63 0.2878006581999699 1106 64-65 0.2044072127426233 1106 66-67 0.2218876097353828 1106 68-69 0.4410728669574979 1106 70-71 0.4052019090988779 1106 72-73 0.3475670726873048 1106 74-75 0.415284251169453 1106 76-77 0.26387875517290027 1106 78-79 0.2649146669187097 1106 80-81 0.6393698109029913 1106 82-83 0.4715559804859488 1106 84-85 0.24435501060363762 1106 86-87 0.314040522240532 1106 88-89 0.09531332300923623 1106 90-91 0.34368364612292623 1106 92-93 0.5702573058353835 1106 94-95 0.6748028918181319 1106 96-97 0.6757900772896086 1106 98-99 0.6714389651842403 1106 100-101 0.8367610519242099 1107 1 0.1260333699675229 1107 2 0.12345800649824668 1107 3 0.14799758682085695 1107 4 0.13483641907067323 1107 5 0.1390022186711306 1107 6 0.10166571761019583 1107 7 0.11483292263052647 1107 8 0.11635213036930026 1107 9 0.13625808533042516 1107 10-11 0.19779420504785605 1107 12-13 0.19931038211517915 1107 14-15 0.26670669621873344 1107 16-17 0.3081224291660831 1107 18-19 0.3185006313578853 1107 20-21 0.24504235447693645 1107 22-23 0.19363220729960773 1107 24-25 0.2539973500160002 1107 26-27 0.25009158301488554 1107 28-29 0.3104902405804637 1107 30-31 0.3029138394682178 1107 32-33 0.31342967247785936 1107 34-35 0.2528481830496858 1107 36-37 0.24436207158574774 1107 38-39 0.2262854976990738 1107 40-41 0.2358466339416907 1107 42-43 0.24926139600297148 1107 44-45 0.17391656123013632 1107 46-47 0.27957899525524255 1107 48-49 0.44244973851741776 1107 50-51 0.4173528861824778 1107 52-53 0.35045211501581974 1107 54-55 0.442975553720963 1107 56-57 0.41170965151869865 1107 58-59 0.4984900573717752 1107 60-61 0.4007708062004198 1107 62-63 0.45982211901982595 1107 64-65 0.3220203663377035 1107 66-67 0.3338884270467375 1107 68-69 0.3356402676741084 1107 70-71 0.29870072791089086 1107 72-73 0.25903808635657555 1107 74-75 0.30829332287385114 1107 76-77 0.35223332906845783 1107 78-79 0.37187549166563016 1107 80-81 0.5298853680406559 1107 82-83 0.5449260426573019 1107 84-85 0.5328126703387106 1107 86-87 0.4551061276567623 1107 88-89 0.3547725165201179 1107 90-91 0.1774534495542781 1107 92-93 0.23935608837261668 1107 94-95 0.2608499346336579 1107 96-97 0.40391486327219184 1107 98-99 0.6323143236986901 1107 100-101 0.9138367668646765 1108 1 0.10445969472255712 1108 2 0.04894210412373923 1108 3 -0.006002141570775166 1108 4 0.07801934435180158 1108 5 0.03273323191794475 1108 6 0.08416616783058117 1108 7 0.05842473659060232 1108 8 0.15393889005198957 1108 9 0.11479216775175871 1108 10-11 0.08983799894433986 1108 12-13 0.1121860947180977 1108 14-15 0.15054503905344063 1108 16-17 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-1.0320396606625977 2316 76-77 -0.991663269180389 2316 78-79 -0.8268282916564154 2316 80-81 -0.8151422951417118 2316 82-83 -0.904304380620502 2316 84-85 -1.0520571528912903 2316 86-87 -0.8915551977717264 2316 88-89 -0.9681288435863493 2316 90-91 -1.1854074906262078 2316 92-93 -1.3144128080842066 2316 94-95 -1.2566792736421029 2316 96-97 -1.3351271717536939 2316 98-99 -1.438552074161798 2316 100-101 -1.8629522179276456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 10.0 5 43.0 6 78.0 7 241.0 8 547.0 9 1070.0 10 1878.0 11 3194.0 12 4830.0 13 6940.0 14 8771.0 15 11937.0 16 16155.0 17 21922.0 18 19154.0 19 11519.0 20 10002.0 21 10535.0 22 10928.0 23 12315.0 24 14214.0 25 17131.0 26 21136.0 27 26331.0 28 33355.0 29 41973.0 30 52817.0 31 67287.0 32 85143.0 33 111699.0 34 152275.0 35 215937.0 36 330431.0 37 504211.0 38 382144.0 39 28494.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65451146649494 20.036358269829343 15.23885549711996 22.07027476655576 2 15.275997333320218 21.498250159066288 40.032345341291645 23.193407166321855 3 19.182774082427283 25.857566744349512 30.37630273773902 24.583356435484188 4 12.693481149503812 17.717597552055484 38.28521379275811 31.3037075056826 5 13.248219796453634 38.15094909910031 34.27572565147447 14.325105452971595 6 30.28948599112782 37.57890134102101 17.970504154867047 14.161108512984127 7 27.46025983455644 33.638282899366644 21.35354864900874 17.547908617068174 8 26.00642657258553 34.79022878401682 20.209008915561704 18.99433572783595 9 26.439651765227673 16.914924628417833 20.851299173943914 35.794124432410584 10-11 24.324839693577996 27.29353757890134 27.817758133354552 20.563864594166105 12-13 25.803344287215257 24.7555943039814 28.272885789014822 21.168175619788524 14-15 22.723429730302495 25.800436723784724 27.12632351718236 24.349810028730413 16-17 21.466879633738365 29.25778590997037 27.47722321639146 21.798111239899807 18-19 21.3404460850691 27.59929203349897 30.30902778010652 20.751234101325412 20-21 22.363161702177127 26.53305124075725 30.264823944008317 20.838963113057304 22-23 21.92759809966137 26.57261695371747 30.14368784800868 21.35609709861248 24-25 21.783417760939205 26.761326634567855 30.26183624062527 21.19341936386767 26-27 21.817814364363223 26.805404465238645 30.189477317673298 21.187303852724835 28-29 21.377213446621855 27.047683349560526 30.340055600951942 21.235047602865674 30-31 21.713235210260056 26.65946907633881 30.239987012136094 21.38730870126504 32-33 21.52510496215416 26.860452925024354 30.14298906909227 21.47145304372922 34-35 21.932055052746513 26.81105150531221 30.00946548830205 21.247427953639228 36-37 21.96712881293026 26.547183859088992 30.05365994352291 21.432027384457843 38-39 21.710997127404923 26.92917596606631 29.982725081987116 21.37710182454165 40-41 21.94175538693364 26.83005951449408 29.90214314778464 21.326041950787637 42-43 21.70528615412681 26.939887021008513 29.615022789849597 21.73980403501508 44-45 21.973334668708777 26.82083695401159 29.41607802467568 21.78975035260395 46-47 22.150923875452637 27.25755618656977 28.931495016343924 21.66002492163367 48-49 22.106070059560707 27.39121949561963 28.704537259366848 21.798173185452818 50-51 21.93470041813766 27.472252327656822 28.6792045369188 21.91384271728672 52-53 22.22409510885967 27.655766913544923 28.278253327044013 21.841884650551396 54-55 22.133899603576552 27.358270178239653 28.373751773599803 22.13407844458399 56-57 22.20819070817193 27.53604526806377 28.255296356307163 22.00046766745714 58-59 22.454718015794793 27.299655038051778 28.29555354309744 21.95007340305599 60-61 22.314542686205083 27.402210489912722 28.72534793610186 21.55789888778034 62-63 22.219701093486808 27.375933454123963 28.618169340118772 21.78619611227046 64-65 22.045418267195053 27.553039323478952 28.46508899392399 21.936453415402006 66-67 22.146518100992495 27.60418960387529 28.07657408026311 22.1727182148691 68-69 22.105578716594064 27.35241274334273 28.44988754309209 22.09212099697112 70-71 22.220945468170186 27.396985881758017 28.39867395429931 21.983394695772486 72-73 22.259360381041983 27.29304407005206 28.39276084625518 22.054834702650787 74-75 22.05309839764957 27.80637121751716 27.99371525584086 22.146815128992404 76-77 22.060568259173934 27.496912120917433 28.219447881631115 22.22307173827752 78-79 22.1158025208055 27.522683493499457 28.105085430874592 22.256428554820452 80-81 22.279068124620245 27.674277301197193 27.885977740827695 22.16067683335487 82-83 22.126382433619714 27.645140160381303 28.107706305931696 22.12077110006729 84-85 22.060863428268547 27.569037442706783 27.985243874658778 22.38485525436589 86-87 22.01368651566134 27.53280122897864 27.98803480556224 22.465477449797778 88-89 22.016069826979532 27.44767313447917 27.92455470635523 22.611702332186063 90-91 22.01335317277573 27.34078445083702 28.1223872429047 22.523475133482545 92-93 22.129568774730064 27.328437618760198 28.16576129479355 22.376232311716183 94-95 21.93189279165067 27.19801684270861 28.16934185297515 22.700748512665562 96-97 21.88208021156664 27.26692516366571 28.257783133014925 22.593211491752722 98-99 21.670598145081392 27.311427636355834 28.02454834198318 22.993425876579597 100-101 21.303632465735355 27.528161725091486 28.07834090050455 23.089864908668602 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 925.0 1 641.0 2 406.5 3 460.5 4 389.0 5 354.0 6 648.5 7 1402.5 8 2329.5 9 2796.0 10 2534.5 11 2170.5 12 2142.5 13 2326.0 14 2531.0 15 2689.5 16 2895.5 17 3121.5 18 3433.5 19 3760.5 20 4140.0 21 4710.0 22 5571.0 23 6584.5 24 7706.5 25 9503.0 26 12384.5 27 15779.5 28 18624.5 29 21973.5 30 26663.5 31 31677.5 32 37165.5 33 42967.5 34 49401.0 35 54616.5 36 60748.0 37 68002.5 38 71328.0 39 71636.0 40 72392.0 41 74824.5 42 77130.0 43 79727.0 44 83465.0 45 85883.5 46 85339.5 47 88042.5 48 95789.5 49 93877.5 50 82839.5 51 75577.5 52 71411.0 53 68609.0 54 64845.0 55 59167.5 56 53245.5 57 47245.5 58 42714.0 59 38187.5 60 33025.0 61 27019.5 62 20995.5 63 16032.5 64 11697.0 65 8690.0 66 6342.5 67 4740.0 68 3220.0 69 1914.0 70 1224.0 71 813.5 72 578.0 73 446.0 74 339.0 75 242.0 76 174.0 77 87.5 78 37.0 79 18.5 80 13.0 81 6.0 82 3.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025663334606067813 2 0.007377090958190225 3 0.00938902485587847 4 8.94192843416997E-5 5 0.005007479923135183 6 0.0 7 0.0 8 1.3412892651254956E-4 9 8.94192843416997E-5 10-11 0.0 12-13 1.788385686833994E-4 14-15 0.0 16-17 6.706446325627478E-5 18-19 0.004806286533366359 20-21 0.0016095471181505946 22-23 0.0 24-25 0.005476931165929106 26-27 0.00594638240872303 28-29 0.008114800054009249 30-31 0.032213297184097316 32-33 6.482898114773229E-4 34-35 0.004828641354451784 36-37 0.015603665117626599 38-39 0.007801832558813299 40-41 0.010014959846270367 42-43 0.00590167276655218 44-45 0.003554416552582563 46-47 0.0019225146133465436 48-49 0.0012742248018692208 50-51 0.0031296749519594894 52-53 0.0026378688880801414 54-55 0.0013636440862109204 56-57 9.836121277586967E-4 58-59 0.0013636440862109204 60-61 2.2354821085424925E-5 62-63 6.706446325627478E-5 64-65 0.006840575252140027 66-67 0.0010059669488441216 68-69 8.718380223315721E-4 70-71 0.003554416552582563 72-73 0.011624506964420961 74-75 0.005678124555697931 76-77 0.0038673840477785122 78-79 0.010104379130612066 80-81 1.3412892651254956E-4 82-83 0.004873350996622634 84-85 0.0010506765910149715 86-87 0.0 88-89 0.001788385686833994 90-91 0.0014307085494671952 92-93 1.788385686833994E-4 94-95 8.271283801607222E-4 96-97 2.9061267411052406E-4 98-99 2.682578530250991E-4 100-101 8.494832012461472E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236654.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.49071274439399 #Duplication Level Percentage of deduplicated Percentage of total 1 73.5755269883618 38.62031852163506 2 14.590781996771291 15.317610930171943 3 5.0489748845613 7.950728709575008 4 2.288539451105248 4.805082677287146 5 1.225238635344692 3.215682462560576 6 0.7331270102996794 2.3089415581678105 7 0.4969458691688476 1.8259530007638622 8 0.3523390944515329 1.479562415638025 9 0.2450486155788869 1.1576498839886504 >10 1.2926988184941348 12.340952645484027 >50 0.09797194976733843 3.561873439032585 >100 0.0495621410323175 4.703346711676023 >500 0.001963803590734048 0.6659958041265022 >1k 0.0010245931777741791 0.9704446262954207 >5k 1.7076552962902986E-4 0.6010993085426585 >10k+ 8.538276481451493E-5 0.47475730505460634 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10593 0.47360923951581246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8057 0.36022558697053725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5355 0.23942013382490093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4929 0.22037382626011892 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2288 0.10229566128690445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2354821085424925E-5 0.0 14-15 0.0 0.0 0.0 4.470964217084985E-5 0.0 16-17 0.0 0.0 0.0 1.3412892651254956E-4 0.0 18-19 0.0 0.0 0.0 2.0119338976882432E-4 0.0 20-21 0.0 0.0 0.0 3.800319584522237E-4 0.0 22-23 0.0 0.0 0.0 4.470964217084985E-4 0.0 24-25 0.0 0.0 0.0 6.929994536481728E-4 0.0 26-27 0.0 0.0 0.0 0.001184805517527521 0.0 28-29 0.0 0.0 0.0 0.002414320677225892 0.0 30-31 0.0 0.0 0.0 0.005342802239416557 0.0 32-33 0.0 0.0 0.0 0.00858425129680317 0.0 34-35 0.0 0.0 0.0 0.012071603386129458 0.0 36-37 0.0 0.0 0.0 0.014262375852501102 0.0 38-39 0.0 0.0 0.0 0.01611782600259137 0.0 40-41 0.0 0.0 0.0 0.018889823817184064 0.0 42-43 0.0 0.0 0.0 0.02228775662216865 0.0 44-45 0.0 0.0 0.0 0.025842173174751213 0.0 46-47 0.0 0.0 0.0 0.03073787899245927 0.0 48-49 0.0 0.0 0.0 0.036550132474669754 0.0 50-51 0.0 0.0 0.0 0.04551441572992515 0.0 52-53 0.0 0.0 0.0 0.06346533706152135 0.0 54-55 0.0 0.0 0.0 0.08340583746972038 0.0 56-57 0.0 0.0 0.0 0.11181881506929547 0.0 58-59 0.0 0.0 0.0 0.14982201091451786 0.0 60-61 0.0 0.0 0.0 0.204121871331015 0.0 62-63 0.0 0.0 0.0 0.24140971290150376 0.0 64-65 0.0 0.0 0.0 0.27959174731540953 0.0 66-67 0.0 0.0 0.0 0.3602479417916227 0.0 68-69 0.0 0.0 0.0 0.46435434358644656 0.0 70-71 0.0 0.0 0.0 0.5339449016253743 0.0 72-73 0.0 0.0 0.0 0.5972537549392978 0.0 74-75 0.0 0.0 0.0 0.7402575454227609 0.0 76-77 0.0 0.0 0.0 0.839691789610731 0.0 78-79 0.0 0.0 0.0 0.9120767002853369 0.0 80-81 0.0 0.0 0.0 1.004670369221167 0.0 82-83 0.0 0.0 0.0 1.173292784668527 0.0 84-85 0.0 0.0 0.0 1.3545009643869816 0.0 86-87 0.0 0.0 0.0 1.5986379654609073 0.0 88-89 0.0 0.0 0.0 1.913215007775007 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17355 0.0 25.10726 1 GTACATG 10610 0.0 24.27384 1 GGTATCA 12860 0.0 22.87201 1 TACATGG 11195 0.0 22.82847 2 GAGTACT 9410 0.0 22.081049 12-13 CCCTACG 130 2.9353448E-4 21.931208 1 ACATGGG 10925 0.0 21.782404 3 GTATACG 110 0.002872023 21.598915 1 TCAACGC 20335 0.0 21.253878 4 ATCAACG 20355 0.0 21.188698 3 CAACGCA 20545 0.0 21.036633 5 TATCAAC 20840 0.0 20.85653 2 AACGCAG 20990 0.0 20.6576 6 GTACTTT 9970 0.0 20.60376 14-15 AGTACTT 9815 0.0 20.540861 12-13 AGAGTAC 16470 0.0 19.334225 10-11 CATGGGG 4380 0.0 19.084444 4 ACGCAGA 23155 0.0 18.623558 7 CGCAGAG 23150 0.0 18.401915 8 TACTTTT 11415 0.0 17.641903 14-15 >>END_MODULE