FastQCFastQC Report
Thu 26 May 2016
SRR936897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267561
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89960.39672582126787326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62340.274920939282339No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44400.19580509631273424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.18350112742281244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24900.10980961482403341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153050.027.6966951
GTACATG113200.026.2799381
TACATGG118450.024.7557052
ACATGGG116500.023.8646533
GGTATCA109650.023.79361
ATCAACG178300.023.4827943
CAACGCA177700.023.4818485
TCAACGC178050.023.462384
TATCAAC182100.023.0449582
AACGCAG181800.022.9753786
GAGTACT77050.022.320712-13
ACGCAGA203600.020.328187
GTACTTT84100.020.30589114-15
CGCAGAG203250.020.1293958
AGTACTT84250.019.84927412-13
AGAGTAC147400.019.71080610-11
CATGGGA76100.019.5472154
CATGGGG47150.019.4536364
GCAGAGT210250.019.0281189
GTATAGG7300.018.878681
Produced by FastQC (version 0.11.5)