##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936897_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2267561 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.716519643793486 33.0 31.0 34.0 30.0 34.0 2 31.828911328074525 33.0 31.0 34.0 30.0 34.0 3 31.83622447202082 33.0 31.0 34.0 30.0 34.0 4 35.41482720861754 37.0 35.0 37.0 33.0 37.0 5 35.40741219310087 37.0 35.0 37.0 33.0 37.0 6 35.519714794883136 37.0 35.0 37.0 33.0 37.0 7 35.46608095658728 37.0 35.0 37.0 33.0 37.0 8 35.420583613847654 37.0 35.0 37.0 33.0 37.0 9 37.013950231107344 39.0 37.0 39.0 33.0 39.0 10-11 36.889540127035175 39.0 37.0 39.0 33.0 39.0 12-13 36.783095361050925 39.0 37.0 39.0 32.5 39.0 14-15 37.92796423117173 40.0 38.0 41.0 33.0 41.0 16-17 37.85379775891365 40.0 38.0 41.0 33.0 41.0 18-19 37.80360704739586 40.0 37.5 41.0 32.0 41.0 20-21 37.688690403477565 40.0 37.0 41.0 32.0 41.0 22-23 37.5640578136597 40.0 37.0 41.0 32.0 41.0 24-25 37.688227350884944 40.0 37.0 41.0 32.0 41.0 26-27 37.64303893037497 40.0 37.0 41.0 32.0 41.0 28-29 37.55120325318701 40.0 37.0 41.0 32.0 41.0 30-31 37.3799549383677 40.0 37.0 41.0 31.5 41.0 32-33 37.190967078724675 40.0 36.0 41.0 31.0 41.0 34-35 37.013570527981386 39.0 36.0 41.0 31.0 41.0 36-37 36.82510944578779 39.0 36.0 41.0 30.0 41.0 38-39 36.66668085224609 39.0 35.5 41.0 30.0 41.0 40-41 36.49256778538703 39.0 35.0 41.0 30.0 41.0 42-43 36.268973579983076 39.0 35.0 40.5 30.0 41.0 44-45 36.005370307568356 39.0 35.0 40.0 29.0 41.0 46-47 35.66200225705064 39.0 35.0 40.0 27.5 41.0 48-49 35.41481133252866 38.5 35.0 40.0 27.0 41.0 50-51 34.70552479073331 38.0 34.0 39.5 25.5 40.5 52-53 34.90569338597727 38.0 34.5 39.5 26.5 40.5 54-55 35.31992391825402 39.0 35.0 40.0 27.0 41.0 56-57 35.229506064004454 39.0 35.0 40.0 26.5 41.0 58-59 34.979862945252634 38.0 34.5 40.0 26.0 41.0 60-61 34.69079707227281 38.0 34.0 40.0 26.0 41.0 62-63 34.35993320576602 38.0 34.0 40.0 25.0 41.0 64-65 34.03868010606991 37.0 34.0 40.0 24.5 41.0 66-67 33.65994674454183 36.5 33.0 39.5 23.0 41.0 68-69 33.26218103945164 36.0 33.0 39.0 22.0 41.0 70-71 32.861351910709345 35.5 32.5 39.0 22.0 40.5 72-73 32.46114680046094 35.0 32.0 38.0 21.0 40.0 74-75 32.0124415616603 35.0 31.5 37.0 20.0 39.5 76-77 31.57191691866283 35.0 31.0 37.0 18.5 39.0 78-79 31.134481938964377 35.0 31.0 36.0 17.5 39.0 80-81 30.683147002440066 35.0 31.0 36.0 12.5 37.5 82-83 30.26463830520987 34.0 30.0 35.5 8.5 37.0 84-85 29.869202416164327 34.0 30.0 35.0 7.0 37.0 86-87 29.531329256412505 34.0 29.5 35.0 3.5 36.0 88-89 29.17153849444403 34.0 29.0 35.0 2.0 36.0 90-91 28.782530657389152 34.0 29.0 35.0 2.0 35.5 92-93 28.41191857683211 34.0 29.0 35.0 2.0 35.0 94-95 27.98900713145093 34.0 28.5 35.0 2.0 35.0 96-97 27.463810896377208 34.0 27.0 35.0 2.0 35.0 98-99 26.81366675472016 33.5 25.5 35.0 2.0 35.0 100-101 24.201294474547765 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5054201016901771 1101 2 0.42806687511371067 1101 3 0.4287788104250687 1101 4 0.4047221231350804 1101 5 0.5630318010238966 1101 6 0.3387121540035949 1101 7 0.35943130784626476 1101 8 0.3526928282234607 1101 9 0.5463334548997025 1101 10-11 0.6135256874998376 1101 12-13 0.6600495879975696 1101 14-15 0.8556805183737737 1101 16-17 0.799788862601865 1101 18-19 0.8293268153932871 1101 20-21 0.8645845385372155 1101 22-23 0.8484622661217642 1101 24-25 0.7621279658954379 1101 26-27 0.5966578513562553 1101 28-29 0.694905154051547 1101 30-31 0.7601195586273732 1101 32-33 0.8049646140301903 1101 34-35 0.7041422497319161 1101 36-37 0.5573113762210156 1101 38-39 0.6978902976345651 1101 40-41 0.6546177325055069 1101 42-43 0.7341696622495206 1101 44-45 0.8294805060564769 1101 46-47 0.8728812489128259 1101 48-49 0.8727483134491436 1101 50-51 0.9164308837792632 1101 52-53 0.9404416599093182 1101 54-55 0.9793501812307213 1101 56-57 0.8527395681546039 1101 58-59 0.8137015509943879 1101 60-61 0.9706805205013609 1101 62-63 1.1765075747689124 1101 64-65 1.079170655995405 1101 66-67 1.1221059969157352 1101 68-69 1.1782085444744226 1101 70-71 1.0424558501351484 1101 72-73 0.9532136921366501 1101 74-75 1.0717010329810819 1101 76-77 0.9896778784763427 1101 78-79 0.9185422478761218 1101 80-81 0.9325416241402529 1101 82-83 0.9021640360590162 1101 84-85 1.019865636164056 1101 86-87 1.071871270449904 1101 88-89 1.162777664823814 1101 90-91 1.0922581935783953 1101 92-93 1.0335191300924116 1101 94-95 1.0997468637761614 1101 96-97 0.9988532102075851 1101 98-99 0.7729747298763456 1101 100-101 1.2184471298583155 1102 1 0.2562829757853322 1102 2 0.3360881272081535 1102 3 0.33912663011869526 1102 4 0.310881847998715 1102 5 0.43261677241242324 1102 6 0.37538569725838045 1102 7 0.30314685519513773 1102 8 0.40274692359475495 1102 9 0.41667414534010305 1102 10-11 0.5510469563248108 1102 12-13 0.4137388968720117 1102 14-15 0.4577664395327048 1102 16-17 0.6759370946176304 1102 18-19 0.7177583273298893 1102 20-21 0.6750076342219486 1102 22-23 0.6517911630200643 1102 24-25 0.22998247723088383 1102 26-27 0.47343239691757333 1102 28-29 0.5088500826190199 1102 30-31 0.4050638550768326 1102 32-33 0.6170688431660238 1102 34-35 0.46282715745338265 1102 36-37 0.4694921560547911 1102 38-39 0.5604550487582927 1102 40-41 0.5771171503514481 1102 42-43 0.5528434117336616 1102 44-45 0.3113790999410284 1102 46-47 0.49563517563584725 1102 48-49 0.6385745385846846 1102 50-51 0.640270200609244 1102 52-53 0.6787144139384722 1102 54-55 0.8142119108212356 1102 56-57 0.8552631462475873 1102 58-59 0.7602411430472316 1102 60-61 0.7535919144911674 1102 62-63 0.7806368965087884 1102 64-65 0.8663278480620775 1102 66-67 0.7564071741757914 1102 68-69 0.9239419755049241 1102 70-71 0.8126968858162371 1102 72-73 0.66275853757503 1102 74-75 0.8045412596330692 1102 76-77 0.7592679031669576 1102 78-79 0.7648807776884432 1102 80-81 0.6716817478824133 1102 82-83 0.6400376475106206 1102 84-85 0.6595115908932989 1102 86-87 0.6221295465409327 1102 88-89 0.7098754646570562 1102 90-91 0.5698345069759938 1102 92-93 0.5436479278367266 1102 94-95 0.6755342171820296 1102 96-97 0.6838856708447523 1102 98-99 0.7192824846236618 1102 100-101 0.7059390690447813 1103 1 0.12930685128415575 1103 2 0.2708807133078217 1103 3 0.21797185727872304 1103 4 0.23864170658028883 1103 5 0.28547884679968405 1103 6 0.1838998298706187 1103 7 0.16871087064829737 1103 8 0.22660408945903754 1103 9 0.3079074889220692 1103 10-11 0.25616923653775103 1103 12-13 0.3925130838083746 1103 14-15 0.42689799826088404 1103 16-17 0.5658384340059328 1103 18-19 0.602115219154598 1103 20-21 0.5616459611864855 1103 22-23 0.5647528846887937 1103 24-25 0.3978933026533511 1103 26-27 0.534799119156034 1103 28-29 0.5121544715171424 1103 30-31 0.6136388429344279 1103 32-33 0.6458197637925878 1103 34-35 0.5165405050584155 1103 36-37 0.6787832751993079 1103 38-39 0.8573853599539447 1103 40-41 0.5417049356342858 1103 42-43 0.5502586552087507 1103 44-45 0.49499261566185737 1103 46-47 0.6641424588501721 1103 48-49 0.7006933578146857 1103 50-51 0.7608428382830965 1103 52-53 0.7698327309217419 1103 54-55 0.8164624727455916 1103 56-57 0.8088075505250245 1103 58-59 0.8448757664990296 1103 60-61 0.8289492958313929 1103 62-63 0.7831105731140866 1103 64-65 0.8159118871825797 1103 66-67 0.6508296890888232 1103 68-69 0.7493555333661064 1103 70-71 0.838661896596399 1103 72-73 0.7105690536924385 1103 74-75 0.7963703607896377 1103 76-77 0.6934156528278237 1103 78-79 0.6630500914946289 1103 80-81 0.6127091321062643 1103 82-83 0.7337356285296366 1103 84-85 0.8204861278109803 1103 86-87 0.7268518117310343 1103 88-89 0.783772574733117 1103 90-91 0.6547345764248149 1103 92-93 0.7451188933281117 1103 94-95 0.8252483057442355 1103 96-97 0.6756791245772611 1103 98-99 0.7634111310846023 1103 100-101 1.0298745319297673 1104 1 0.44153826883076874 1104 2 0.43220967043805913 1104 3 0.45450730991909793 1104 4 0.44238777383100825 1104 5 0.4725196527315916 1104 6 0.3743528977802626 1104 7 0.3751458057911705 1104 8 0.5364952862360539 1104 9 0.5351077687746866 1104 10-11 0.5873568318679077 1104 12-13 0.6094397652662522 1104 14-15 0.7635720137856126 1104 16-17 0.8011926740905722 1104 18-19 0.7092930453534194 1104 20-21 0.8544078716246872 1104 22-23 0.7817707208133484 1104 24-25 0.706238570820453 1104 26-27 0.6704898524076768 1104 28-29 0.7155352383888882 1104 30-31 0.6290541098997622 1104 32-33 0.6958964953757913 1104 34-35 0.5875649880721951 1104 36-37 0.6728525733921273 1104 38-39 0.6631793687160936 1104 40-41 0.7337873932108465 1104 42-43 0.7164768526018435 1104 44-45 0.6215368156442835 1104 46-47 0.47544421566159656 1104 48-49 0.4944224231618719 1104 50-51 0.452450239808293 1104 52-53 0.4487878452341718 1104 54-55 0.4979718654743621 1104 56-57 0.5604165375181509 1104 58-59 0.680718385240155 1104 60-61 0.6632183279227064 1104 62-63 0.7860904347498732 1104 64-65 0.7674258824429288 1104 66-67 0.6840236220056113 1104 68-69 0.5868584136003605 1104 70-71 0.630409127042995 1104 72-73 0.7209666263293641 1104 74-75 0.7603561842912185 1104 76-77 0.6318946281403015 1104 78-79 0.6861635453585855 1104 80-81 0.6774490072672243 1104 82-83 0.7777684940720242 1104 84-85 0.8235209159766868 1104 86-87 0.7849415838852707 1104 88-89 0.8431551654772953 1104 90-91 0.7754060201413857 1104 92-93 0.7279998326730599 1104 94-95 0.8111617933432065 1104 96-97 0.6664624723336772 1104 98-99 0.4806573408131811 1104 100-101 0.6889684467519608 1105 1 0.23864491068051308 1105 2 0.330750338082229 1105 3 0.28849360327656726 1105 4 0.41425163442496427 1105 5 0.4251916260383055 1105 6 0.30998860811641293 1105 7 0.23544102871152717 1105 8 0.30857373885969963 1105 9 0.302496325718451 1105 10-11 0.41673847269968434 1105 12-13 0.3990509080687019 1105 14-15 0.4139465508943516 1105 16-17 0.6002666632456695 1105 18-19 0.489376362925654 1105 20-21 0.5160838814230786 1105 22-23 0.5378736930214103 1105 24-25 0.3942769774280137 1105 26-27 0.35057048710384464 1105 28-29 0.32222075622392765 1105 30-31 0.18573795331570153 1105 32-33 0.404441969363873 1105 34-35 0.14541588257351634 1105 36-37 0.27945351403511154 1105 38-39 0.44523127038049637 1105 40-41 0.45080457160916154 1105 42-43 0.37083156002184836 1105 44-45 0.5071711369736889 1105 46-47 0.4329933151475913 1105 48-49 0.3982083037750286 1105 50-51 0.41649275559255017 1105 52-53 0.4877687975752494 1105 54-55 0.5731792555991788 1105 56-57 0.6321782395195044 1105 58-59 0.6938210001119671 1105 60-61 0.4321746548865235 1105 62-63 0.6130567260295336 1105 64-65 0.7918768727666858 1105 66-67 0.6401889472976734 1105 68-69 0.8444611325525457 1105 70-71 0.6611494638371695 1105 72-73 0.5579099818728537 1105 74-75 0.5289470883138989 1105 76-77 0.6568937053070414 1105 78-79 0.7112541447511482 1105 80-81 0.688532234649152 1105 82-83 0.7001271074551205 1105 84-85 0.6075000169430211 1105 86-87 0.6609576042705605 1105 88-89 0.7688328390394013 1105 90-91 0.7916856549810234 1105 92-93 0.8435570127534042 1105 94-95 0.8515392682963743 1105 96-97 0.695072205960237 1105 98-99 0.40793760470163676 1105 100-101 0.6120075783068835 1106 1 0.11310001094206612 1106 2 0.30982618812582174 1106 3 0.3163924698851126 1106 4 0.3231443981564368 1106 5 0.3480338230740543 1106 6 0.28732077899697117 1106 7 0.33439482121370645 1106 8 0.3447550814926501 1106 9 0.4594274503915088 1106 10-11 0.44376549972670887 1106 12-13 0.35698464826050724 1106 14-15 0.5928916250007177 1106 16-17 0.37511409131889906 1106 18-19 0.3524975486274826 1106 20-21 0.6050115187378182 1106 22-23 0.4825118795950871 1106 24-25 0.4005978143940112 1106 26-27 0.43928016452319696 1106 28-29 0.5194744615421456 1106 30-31 0.5662959306478754 1106 32-33 0.47702261452516836 1106 34-35 0.46363732982722183 1106 36-37 0.4335511600682409 1106 38-39 0.5099653593081328 1106 40-41 0.6258263675986981 1106 42-43 0.39546102819969775 1106 44-45 0.6216000820477916 1106 46-47 0.562243533107484 1106 48-49 0.5950260893024861 1106 50-51 0.4814448044155668 1106 52-53 0.5437844906877203 1106 54-55 0.6034756810568922 1106 56-57 0.631742319728744 1106 58-59 0.5087730489349838 1106 60-61 0.6599427455578422 1106 62-63 0.5533357146956277 1106 64-65 0.701423516184299 1106 66-67 0.6332142306455353 1106 68-69 0.5334323618463586 1106 70-71 0.49332034439515127 1106 72-73 0.48772689556073345 1106 74-75 0.696558247860537 1106 76-77 0.6566757454116683 1106 78-79 0.7914633862507152 1106 80-81 0.6109385118941368 1106 82-83 0.6316877003060419 1106 84-85 0.6478225975881813 1106 86-87 0.7747326696585333 1106 88-89 0.800654983764769 1106 90-91 0.8219820804387403 1106 92-93 0.696434083372413 1106 94-95 0.667799076491665 1106 96-97 0.620529921740534 1106 98-99 0.4465165061837624 1106 100-101 0.42783146671141736 1107 1 0.1590415425103835 1107 2 0.20786804378302648 1107 3 0.2051402213115061 1107 4 0.2179151999913458 1107 5 0.28910629611876004 1107 6 0.21535391625836553 1107 7 -9.636078564412287E-4 1107 8 0.1216837046850614 1107 9 0.11257030333019458 1107 10-11 0.10536206975529439 1107 12-13 0.20698814297612955 1107 14-15 0.1915292377052964 1107 16-17 0.3568161281863098 1107 18-19 0.2218639750784348 1107 20-21 0.3959545033721028 1107 22-23 0.35547216123238456 1107 24-25 0.2634042917574533 1107 26-27 0.13628353784343972 1107 28-29 0.22582914206995497 1107 30-31 0.3334262908978687 1107 32-33 0.2476351829940029 1107 34-35 0.12759117750912452 1107 36-37 0.2775448475082314 1107 38-39 0.14118751654091 1107 40-41 0.29077124305806734 1107 42-43 0.30523721925396075 1107 44-45 0.10788920906782806 1107 46-47 0.21486659308047962 1107 48-49 0.2269118746386667 1107 50-51 0.05316317448019703 1107 52-53 0.3058007944557559 1107 54-55 0.3137291766921777 1107 56-57 0.3074047522715375 1107 58-59 0.31968244751281816 1107 60-61 0.3555360930907554 1107 62-63 0.2961879176539881 1107 64-65 0.30774306562349096 1107 66-67 0.41257738309974457 1107 68-69 0.42972906688132184 1107 70-71 0.29010649354961515 1107 72-73 0.1755934268531547 1107 74-75 0.39539910586840676 1107 76-77 0.3113577446832956 1107 78-79 0.24426044627521293 1107 80-81 0.3064319841332228 1107 82-83 0.17317806776619094 1107 84-85 0.12371733675364283 1107 86-87 0.16523403259762404 1107 88-89 0.0898305619562727 1107 90-91 0.05744443131554533 1107 92-93 0.07827627672950754 1107 94-95 0.09554270415362609 1107 96-97 -0.07329454789038792 1107 98-99 -0.1499624758607574 1107 100-101 -0.19736212214080595 1108 1 -0.03222930932522061 1108 2 0.11999804296268834 1108 3 0.09234075203686487 1108 4 0.08879200251372055 1108 5 0.21585763630729815 1108 6 0.2072810413889954 1108 7 0.11476162763510445 1108 8 0.1788026649198926 1108 9 0.225612308387781 1108 10-11 0.15711046741410684 1108 12-13 0.1720767220374455 1108 14-15 0.27729969898695117 1108 16-17 0.21556007816687384 1108 18-19 0.3958011868442739 1108 20-21 0.418913011919706 1108 22-23 0.35911875806300486 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-2.3406951243958183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3790.0 3 1437.0 4 1471.0 5 1642.0 6 1896.0 7 2277.0 8 2934.0 9 3519.0 10 4579.0 11 5809.0 12 7561.0 13 9523.0 14 10787.0 15 12806.0 16 16236.0 17 21342.0 18 18686.0 19 12296.0 20 12218.0 21 13060.0 22 14588.0 23 16401.0 24 18391.0 25 21811.0 26 25122.0 27 30067.0 28 36223.0 29 43977.0 30 53762.0 31 66830.0 32 84930.0 33 109825.0 34 149673.0 35 210297.0 36 311402.0 37 450655.0 38 397195.0 39 62526.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49047968786874 19.555137968985374 14.555832721289635 22.398549621856244 2 14.990857055151407 20.982330696742675 40.688971394446845 23.337840853659074 3 18.959248976207434 25.93951850324504 29.938759893353563 25.16247262719396 4 12.24109282569582 17.26262495715849 38.665426891102506 31.830855326043185 5 13.333442176198359 38.07544046059947 34.19557474327792 14.395542619924246 6 30.141974137588733 37.25554903578472 18.377268255235794 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27.37118921531617 29.55752825122866 21.554240947787704 30-31 21.78470612256958 27.077176755112653 29.494421539266195 21.64369558305157 32-33 21.56346510974606 27.24944025564121 29.425287964603253 21.761806670009477 34-35 22.051698742260402 27.230282770907937 29.26026213198328 21.457756354848385 36-37 22.037621038640197 26.917600011642467 29.256721208382046 21.788057741335294 38-39 21.76093323260828 27.343544313510698 29.282259028320762 21.613263425560262 40-41 22.01248611667571 27.184679028126506 29.198673374909625 21.604161480288163 42-43 21.718178005511653 27.289543513531513 28.958959833018678 22.033318647938156 44-45 22.008000231085447 27.22168922138537 28.754747119094336 22.015563428434845 46-47 22.161194817073238 27.529056005341424 28.408304872130202 21.901444305455136 48-49 22.07405728327267 27.600801832634307 28.282659454200793 22.042481429892234 50-51 21.887297110764678 27.716615473494578 28.283569748032573 22.112517667708172 52-53 22.211968865074642 27.90300103636083 27.825163722960905 22.059866375603626 54-55 22.045656107539656 27.63271455076191 27.983604974075167 22.338024367623262 56-57 22.103119180119872 27.898887389963882 27.836595516484525 22.161397913431717 58-59 22.37302031110531 27.619967647588034 27.852180877149035 22.15483116415762 60-61 22.29517414745477 27.657438340003747 28.25254964222318 21.794837870318307 62-63 22.0928778752148 27.695601045113694 28.240781881579508 21.970739198091998 64-65 21.992840642780976 27.832519375776727 28.006321438116146 22.16831854332615 66-67 22.0705105883645 27.859328141053812 27.722931014369003 22.347230256212686 68-69 21.98572113180309 27.691490349424942 28.084332346137618 22.23845617263435 70-71 22.16370408487135 27.666472496634743 27.973762563062067 22.196060855431845 72-73 22.18686938859226 27.512774877654493 28.01333836168397 22.287017372069275 74-75 21.869755173425258 28.125900097701262 27.684943198734956 22.319401530138524 76-77 22.02159774237905 27.757982469645682 27.809376255894293 22.41104353208097 78-79 22.02189075266996 27.82171837203517 27.77494005494302 22.38145082035185 80-81 22.08731674258455 28.0101690548003 27.583230236789667 22.31928396582548 82-83 22.104004441659217 27.894572798013694 27.741245557182726 22.260177203144366 84-85 22.07271602885281 27.861472820038703 27.60896884247801 22.456842308630478 86-87 21.955459186137624 27.872732088435896 27.699633226248306 22.47217549917817 88-89 22.05086258474837 27.748506820999136 27.53575789626665 22.664872697985842 90-91 21.98634431841466 27.632408654994546 27.830933689638826 22.55031333695197 92-93 22.203794343996698 27.65909577178351 27.808006458457868 22.32910342576192 94-95 22.046349808158563 27.49880140452895 27.829600522574395 22.625248264738094 96-97 21.831657489074225 27.546870120452592 27.96228777001265 22.659184620460532 98-99 21.75017788602149 27.62904070358608 27.732463834988824 22.8883175754036 100-101 21.79720440458706 27.57494914271672 27.73143648497752 22.896409967718697 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 657.0 1 447.0 2 263.5 3 316.0 4 336.0 5 363.5 6 590.5 7 1111.5 8 1850.0 9 2272.0 10 2130.5 11 1975.0 12 2073.5 13 2212.0 14 2357.0 15 2542.5 16 2681.0 17 2842.5 18 3041.5 19 3261.5 20 3651.5 21 4253.0 22 5031.0 23 6036.0 24 7388.0 25 9317.0 26 12260.5 27 15620.0 28 18573.5 29 22065.5 30 26761.5 31 31969.5 32 37738.5 33 43782.5 34 50502.5 35 55914.5 36 61772.0 37 68925.0 38 72257.5 39 72424.0 40 73135.0 41 76069.5 42 78278.0 43 80638.5 44 84554.0 45 87003.5 46 86816.5 47 88749.5 48 95959.0 49 94772.5 50 84554.0 51 77729.5 52 73241.0 53 70228.0 54 66732.5 55 60953.0 56 54828.5 57 48631.5 58 43710.5 59 39179.0 60 33892.5 61 27781.0 62 21764.0 63 16568.5 64 12192.5 65 9151.0 66 6534.0 67 4794.5 68 3367.0 69 2064.0 70 1353.0 71 923.5 72 646.5 73 475.0 74 351.5 75 245.5 76 174.0 77 96.5 78 45.5 79 23.0 80 23.5 81 20.0 82 11.0 83 11.0 84 10.5 85 6.5 86 4.5 87 5.5 88 4.5 89 3.0 90 1.5 91 1.0 92 3.0 93 2.5 94 1.0 95 1.5 96 1.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02496073975518189 2 0.010539959013230515 3 0.011289663210824318 4 0.021344519507964725 5 0.057198020251715394 6 0.04193933481833565 7 0.014685382223455069 8 0.04895127407818356 9 0.028180057780143514 10-11 0.02288802815006961 12-13 0.03629450321292349 14-15 0.01823545210029631 16-17 0.017110895803905606 18-19 0.01702269531007104 20-21 0.014464880988868657 22-23 0.007695493087065795 24-25 0.001808110123608582 26-27 6.615037037592373E-5 28-29 0.002160912098946842 30-31 0.0 32-33 3.5280197533825994E-4 34-35 4.410024691728249E-5 36-37 0.0 38-39 8.820049383456499E-5 40-41 1.1025061729320624E-4 42-43 8.820049383456499E-5 44-45 6.615037037592373E-5 46-47 2.2050123458641246E-5 48-49 2.4255135804505368E-4 50-51 2.6460148150369493E-4 52-53 4.8510271609010735E-4 54-55 0.0019404108643604294 56-57 4.4100246917282496E-4 58-59 0.0014553081482703222 60-61 0.0037926212348862944 62-63 0.002491663950826461 64-65 0.001719909629774017 66-67 0.012546520247966868 68-69 0.016074540001349467 70-71 0.028554909878940413 72-73 0.018345702717589514 74-75 0.019668710125107992 76-77 0.03307518518796187 78-79 0.021388619754882005 80-81 0.047495965929913245 82-83 0.03706625753397594 84-85 0.020638915557288206 86-87 0.015854038766763057 88-89 0.014751532593830993 90-91 0.026129396298489874 92-93 0.03378078913863839 94-95 0.014288480001199526 96-97 0.019624609878190705 98-99 0.02910616296540644 100-101 0.040748628151569016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2267561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.339851645542595 #Duplication Level Percentage of deduplicated Percentage of total 1 74.99021884561628 40.749573669375614 2 13.919945972760402 15.12815598147537 3 4.746277081892538 7.73735977496038 4 2.146324645010106 4.665238511720841 5 1.1623339572764924 3.158052740049052 6 0.6899906937534035 2.2496395161218974 7 0.45679055086058074 1.7375351536794745 8 0.3181485632739887 1.3830516583640853 9 0.23194872041099152 1.1343653150856043 >10 1.196784347718096 11.878537413981299 >50 0.09128456564177917 3.416574683427911 >100 0.04734681610621629 4.532700904648608 >500 0.0016282747994906294 0.6052046435306628 >1k 8.141373997452389E-4 0.9502332264908018 >5k 1.6282747994904778E-4 0.6737768070885475 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8996 0.39672582126787326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6234 0.274920939282339 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4440 0.19580509631273424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4161 0.18350112742281244 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2490 0.10980961482403341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.410024691728249E-5 0.0 4 0.0 0.0 0.0 4.410024691728249E-5 0.0 5 0.0 0.0 0.0 4.410024691728249E-5 0.0 6 0.0 0.0 0.0 4.410024691728249E-5 0.0 7 0.0 0.0 0.0 4.410024691728249E-5 0.0 8 0.0 0.0 0.0 4.410024691728249E-5 0.0 9 0.0 0.0 0.0 4.410024691728249E-5 0.0 10-11 0.0 0.0 0.0 6.615037037592373E-5 0.0 12-13 0.0 0.0 0.0 8.820049383456499E-5 0.0 14-15 0.0 0.0 0.0 1.1025061729320623E-4 0.0 16-17 0.0 0.0 0.0 1.5435086421048872E-4 0.0 18-19 0.0 0.0 0.0 1.984511111277712E-4 0.0 20-21 0.0 0.0 0.0 2.425513580450537E-4 0.0 22-23 0.0 0.0 0.0 4.189523457141837E-4 0.0 24-25 0.0 0.0 0.0 8.599548148870086E-4 0.0 26-27 0.0 0.0 0.0 0.0011907066667666273 0.0 28-29 0.0 0.0 0.0 0.002337313086615972 0.0 30-31 0.0 0.0 0.0 0.004806926913983791 0.0 32-33 0.0 0.0 0.0 0.0077616434574417185 0.0 34-35 0.0 0.0 0.0 0.010363558025561385 0.0 36-37 0.0 0.0 0.0 0.012303968889921815 0.0 38-39 0.0 0.0 0.0 0.014420780741951375 0.0 40-41 0.0 0.0 0.0 0.01647144222360501 0.0 42-43 0.0 0.0 0.0 0.019690760248566634 0.0 44-45 0.0 0.0 0.0 0.022910078273528253 0.0 46-47 0.0 0.0 0.0 0.027386253335632424 0.0 48-49 0.0 0.0 0.0 0.0326121325953304 0.0 50-51 0.0 0.0 0.0 0.0396681721020956 0.0 52-53 0.0 0.0 0.0 0.05693341877021169 0.0 54-55 0.0 0.0 0.0 0.07653597852494376 0.0 56-57 0.0 0.0 0.0 0.10343712914448608 0.0 58-59 0.0 0.0 0.0 0.14015058470312375 0.0 60-61 0.0 0.0 0.0 0.19446003878175713 0.0 62-63 0.0 0.0 0.0 0.23494406545182245 0.0 64-65 6.615037037592373E-5 0.0 0.0 0.2759352449614365 0.0 66-67 8.820049383456499E-5 0.0 0.0 0.35335322842472594 0.0 68-69 8.820049383456499E-5 0.0 0.0 0.4537474405319195 0.0 70-71 8.820049383456499E-5 0.0 0.0 0.5275492037479919 0.0 72-73 8.820049383456499E-5 0.0 0.0 0.5940964763461711 0.0 74-75 8.820049383456499E-5 0.0 0.0 0.7323066501849343 0.0 76-77 8.820049383456499E-5 0.0 0.0 0.8351704761194958 0.0 78-79 8.820049383456499E-5 0.0 0.0 0.9103393469900039 0.0 80-81 8.820049383456499E-5 0.0 0.0 1.0029719156397556 0.0 82-83 8.820049383456499E-5 0.0 0.0 1.1743013749133981 0.0 84-85 8.820049383456499E-5 0.0 0.0 1.3491368038169647 0.0 86-87 8.820049383456499E-5 0.0 0.0 1.586484332725779 0.0 88-89 8.820049383456499E-5 0.0 0.0 1.888571024109164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15305 0.0 27.696695 1 GTACATG 11320 0.0 26.279938 1 TACATGG 11845 0.0 24.755705 2 ACATGGG 11650 0.0 23.864653 3 GGTATCA 10965 0.0 23.7936 1 ATCAACG 17830 0.0 23.482794 3 CAACGCA 17770 0.0 23.481848 5 TCAACGC 17805 0.0 23.46238 4 TATCAAC 18210 0.0 23.044958 2 AACGCAG 18180 0.0 22.975378 6 GAGTACT 7705 0.0 22.3207 12-13 ACGCAGA 20360 0.0 20.32818 7 GTACTTT 8410 0.0 20.305891 14-15 CGCAGAG 20325 0.0 20.129395 8 AGTACTT 8425 0.0 19.849274 12-13 AGAGTAC 14740 0.0 19.710806 10-11 CATGGGA 7610 0.0 19.547215 4 CATGGGG 4715 0.0 19.453636 4 GCAGAGT 21025 0.0 19.028118 9 GTATAGG 730 0.0 18.87868 1 >>END_MODULE