FastQCFastQC Report
Thu 26 May 2016
SRR936897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267561
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105120.46358179559447354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80510.35505108793104134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53310.23509841631603295No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51060.2251758607596444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167400.026.8189451
GTACATG112050.023.5737251
GGTATCA121850.023.0423741
TACATGG115550.022.52532
TATCAAC202950.022.1392042
ATCAACG201500.022.1330323
TCAACGC201500.022.1330324
GAGTACT95900.022.11328312-13
CAACGCA202800.021.951095
AACGCAG207700.021.4789756
ACATGGG114750.021.0676173
GTACTTT103350.020.35839814-15
AGTACTT101500.020.28492712-13
GTATAGG7000.019.681821
AGAGTAC166250.019.6183910-11
ACGCAGA227700.019.3211257
CGCAGAG229650.019.1363778
GCAGAGT238000.018.1056779
TACTTTT115700.017.67218214-15
CATGGGA74100.017.4982594
Produced by FastQC (version 0.11.5)