##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936897_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2267561 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16341522896187 33.0 31.0 34.0 31.0 34.0 2 32.284173171085584 33.0 31.0 34.0 30.0 34.0 3 32.397344106729655 34.0 31.0 34.0 31.0 34.0 4 36.04993029955975 37.0 35.0 37.0 35.0 37.0 5 35.9592518128509 37.0 35.0 37.0 35.0 37.0 6 36.000581682256836 37.0 35.0 37.0 35.0 37.0 7 35.948116059501814 37.0 35.0 37.0 35.0 37.0 8 35.90903001065903 37.0 35.0 37.0 35.0 37.0 9 37.59147603967435 39.0 37.0 39.0 35.0 39.0 10-11 37.502252420111304 39.0 37.0 39.0 35.0 39.0 12-13 37.406362386723 39.0 37.0 39.0 34.0 39.0 14-15 38.59817905670454 40.0 38.0 41.0 34.0 41.0 16-17 38.517882870626195 40.0 38.0 41.0 34.0 41.0 18-19 38.68482259132169 40.0 38.0 41.0 34.0 41.0 20-21 38.71766404520099 40.0 38.0 41.0 34.0 41.0 22-23 38.6392432221228 40.0 38.0 41.0 34.0 41.0 24-25 38.53778884007971 40.0 38.0 41.0 34.0 41.0 26-27 38.36134750068466 40.0 38.0 41.0 34.0 41.0 28-29 38.281725607381674 40.0 38.0 41.0 34.0 41.0 30-31 38.266947835140925 40.0 38.0 41.0 34.0 41.0 32-33 38.10399499726799 40.0 37.0 41.0 33.5 41.0 34-35 37.9587940522879 40.0 37.0 41.0 33.0 41.0 36-37 37.7971395256842 40.0 37.0 41.0 33.0 41.0 38-39 37.647312023800026 40.0 37.0 41.0 33.0 41.0 40-41 37.476304275827644 40.0 37.0 41.0 33.0 41.0 42-43 37.22207054187297 40.0 36.5 41.0 32.0 41.0 44-45 36.95376221411463 40.0 36.0 41.0 31.0 41.0 46-47 36.60245898566786 39.0 36.0 41.0 30.5 41.0 48-49 36.246842973573806 39.0 36.0 41.0 30.0 41.0 50-51 35.96879466528134 39.0 35.0 40.0 29.0 41.0 52-53 35.74095669311653 39.0 35.0 40.0 28.5 41.0 54-55 35.464388830113066 38.5 35.0 40.0 28.0 41.0 56-57 35.17850302593844 38.0 34.5 40.0 27.5 41.0 58-59 34.834095973603354 38.0 34.0 40.0 26.5 41.0 60-61 34.507105431783316 38.0 34.0 40.0 26.0 41.0 62-63 34.14857725988408 37.0 33.5 40.0 26.0 41.0 64-65 33.77327026704023 36.5 33.0 39.0 25.0 40.5 66-67 33.37486576987345 36.0 33.0 39.0 24.0 40.0 68-69 33.06276788143737 36.0 32.0 39.0 23.5 40.0 70-71 32.596088925501896 35.0 32.0 38.0 22.5 40.0 72-73 32.094603849686955 35.0 31.0 37.5 21.5 39.5 74-75 31.579494664090625 35.0 31.0 37.0 20.0 39.0 76-77 30.15475526347472 33.5 29.5 35.5 19.5 37.5 78-79 30.917379069405406 34.0 30.5 36.0 19.5 38.0 80-81 30.697035052199258 34.0 31.0 35.5 18.0 37.0 82-83 30.36014907647468 34.0 30.0 35.0 17.0 37.0 84-85 29.987016446305084 34.0 30.0 35.0 13.0 36.5 86-87 29.636602058334923 34.0 29.5 35.0 8.5 36.0 88-89 29.24083100741281 34.0 29.0 35.0 4.5 36.0 90-91 28.768071729933617 34.0 29.0 35.0 2.0 35.0 92-93 28.281866948673045 33.0 29.0 35.0 2.0 35.0 94-95 27.684386439879678 33.0 27.0 35.0 2.0 35.0 96-97 27.00783903939078 33.0 26.0 34.0 2.0 35.0 98-99 26.15509417387228 32.0 25.0 34.0 2.0 35.0 100-101 22.511223953842915 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24161735991373945 1101 2 0.20559870353902454 1101 3 0.2103252175686876 1101 4 0.22778749443797608 1101 5 0.2701101828297183 1101 6 0.31446298405913353 1101 7 0.2886709085936232 1101 8 0.30365286944915226 1101 9 0.3371109257824614 1101 10-11 0.36287020148346727 1101 12-13 0.4482004107985418 1101 14-15 0.5442053780890248 1101 16-17 0.572622825030848 1101 18-19 0.48586232085768444 1101 20-21 0.44685423729315943 1101 22-23 0.4531902711912963 1101 24-25 0.4433314907699213 1101 26-27 0.42211783310457207 1101 28-29 0.5246978180917736 1101 30-31 0.5058843428071285 1101 32-33 0.540128365800463 1101 34-35 0.6410589935920683 1101 36-37 0.5390907537806982 1101 38-39 0.4151456497048329 1101 40-41 0.4530544633054845 1101 42-43 0.4772452691211413 1101 44-45 0.5160195740261528 1101 46-47 0.4689460197012352 1101 48-49 0.4679990639587359 1101 50-51 0.32488677598145443 1101 52-53 0.37778928341419515 1101 54-55 0.351157281918816 1101 56-57 0.4127732145455951 1101 58-59 0.36939561561451484 1101 60-61 0.4867099973678535 1101 62-63 0.49050037502855304 1101 64-65 0.47959286454885586 1101 66-67 0.2401358724487821 1101 68-69 0.28906277326567675 1101 70-71 0.3763659503431711 1101 72-73 0.5096451920502361 1101 74-75 0.524368940154762 1101 76-77 0.43107925281416115 1101 78-79 0.5152026213526568 1101 80-81 0.5884108819552196 1101 82-83 0.6221605621822448 1101 84-85 0.6190595861513373 1101 86-87 0.4025452872914066 1101 88-89 0.41612168682483386 1101 90-91 0.600189110344715 1101 92-93 0.6930583241225179 1101 94-95 0.6834373740148969 1101 96-97 0.7468281764502898 1101 98-99 0.8730171549410528 1101 100-101 1.2577147416654526 1102 1 0.3150158899491302 1102 2 0.32173656035165976 1102 3 0.2904275674224479 1102 4 0.24070232330744545 1102 5 0.28614576718150175 1102 6 0.21862984136276253 1102 7 0.17157292438314187 1102 8 0.2485083689100236 1102 9 0.2795606437194351 1102 10-11 0.32927678243066083 1102 12-13 0.41777748355720945 1102 14-15 0.39033107738521977 1102 16-17 0.45430123607235373 1102 18-19 0.3789441226648975 1102 20-21 0.4086537773516241 1102 22-23 0.35106792983710733 1102 24-25 0.40467452644328006 1102 26-27 0.39451223559371584 1102 28-29 0.41852133993831586 1102 30-31 0.4795455990261601 1102 32-33 0.4233280028732125 1102 34-35 0.553510193375736 1102 36-37 0.34753486710486214 1102 38-39 0.4341540324956483 1102 40-41 0.4513400964261862 1102 42-43 0.5036923629830383 1102 44-45 0.39377461953205284 1102 46-47 0.38218184706677505 1102 48-49 0.35481829937592124 1102 50-51 0.4300989156519961 1102 52-53 0.37384600058428674 1102 54-55 0.37047591924810774 1102 56-57 0.3448749306879648 1102 58-59 0.4177337507229666 1102 60-61 0.5102270865201604 1102 62-63 0.47641042579602555 1102 64-65 0.4596285654452643 1102 66-67 0.4433701223646125 1102 68-69 0.4110650782087859 1102 70-71 0.24473699109805835 1102 72-73 0.31821472742186785 1102 74-75 0.324583568918019 1102 76-77 0.30371339123867 1102 78-79 0.30034293129306633 1102 80-81 0.5842889125422701 1102 82-83 0.45123500893102886 1102 84-85 0.4207103087393911 1102 86-87 0.3298230347569593 1102 88-89 0.4377623392288932 1102 90-91 0.5231297816189908 1102 92-93 0.3962549018988675 1102 94-95 0.1926371153404567 1102 96-97 0.43523212285159474 1102 98-99 0.5985137044689885 1102 100-101 1.0196364942416665 1103 1 0.257563063904108 1103 2 0.24468083387944262 1103 3 0.21087333846031697 1103 4 0.1382325297251228 1103 5 0.15427767028926098 1103 6 0.1612903232013565 1103 7 0.14036105837417523 1103 8 0.1344277000624956 1103 9 0.24007857532857457 1103 10-11 0.25761304712805355 1103 12-13 0.3169812092222699 1103 14-15 0.45416765686211136 1103 16-17 0.33748526398937884 1103 18-19 0.3214254125414797 1103 20-21 0.3201886472162485 1103 22-23 0.2966906489589505 1103 24-25 0.2669456110989117 1103 26-27 0.33063141511731686 1103 28-29 0.2834806223047437 1103 30-31 0.338888184139158 1103 32-33 0.4191384757044645 1103 34-35 0.4252911832410433 1103 36-37 0.36815340083719406 1103 38-39 0.29839750185573877 1103 40-41 0.43106148634471 1103 42-43 0.5291026107262695 1103 44-45 0.4823505113064641 1103 46-47 0.35271320375044724 1103 48-49 0.5635906211881334 1103 50-51 0.5167555755774771 1103 52-53 0.36330295663801593 1103 54-55 0.3194851436556476 1103 56-57 0.4299500544513606 1103 58-59 0.3908648231184273 1103 60-61 0.2660616468251078 1103 62-63 0.35679294811863116 1103 64-65 0.2639140223784082 1103 66-67 0.21565851511775236 1103 68-69 0.09182202579625454 1103 70-71 0.11420662744976795 1103 72-73 0.2688439999407066 1103 74-75 0.3624325396941366 1103 76-77 0.24760689201304942 1103 78-79 0.33762918201795244 1103 80-81 0.37990663465913954 1103 82-83 0.39992627104717116 1103 84-85 0.500504839533356 1103 86-87 0.498863756209893 1103 88-89 0.4241399307488827 1103 90-91 0.39478091301094054 1103 92-93 0.26872624232476383 1103 94-95 0.22320764599692566 1103 96-97 0.33401667300338644 1103 98-99 0.2811042664315302 1103 100-101 0.5433608097952103 1104 1 0.29294042377279794 1104 2 0.2865383556294674 1104 3 0.28658508013577944 1104 4 0.21844337748105147 1104 5 0.23070170387936173 1104 6 0.17632947977916302 1104 7 0.2681581480861439 1104 8 0.1701579921021832 1104 9 0.26990056467789003 1104 10-11 0.2970634429204537 1104 12-13 0.31753875245598095 1104 14-15 0.24029173511410562 1104 16-17 0.3732800265304945 1104 18-19 0.39053613449227953 1104 20-21 0.3791113743535135 1104 22-23 0.24772218672064383 1104 24-25 0.29162885350156387 1104 26-27 0.22569632483622115 1104 28-29 0.2264062118543677 1104 30-31 0.348908088189134 1104 32-33 0.2608836512755559 1104 34-35 0.26947279839656346 1104 36-37 0.4208613469336271 1104 38-39 0.45408295281131217 1104 40-41 0.4706926332710424 1104 42-43 0.37769061909614265 1104 44-45 0.23712108494395068 1104 46-47 0.3140248648670081 1104 48-49 0.2198270904172972 1104 50-51 0.4269553737516958 1104 52-53 0.455761154529732 1104 54-55 0.4859915105559054 1104 56-57 0.45331700788515406 1104 58-59 0.33947511374015704 1104 60-61 0.5366320705248455 1104 62-63 0.6137000079501789 1104 64-65 0.47314047180091023 1104 66-67 0.4280179192700757 1104 68-69 0.37739946821056236 1104 70-71 0.3557021210396272 1104 72-73 0.45169139904290745 1104 74-75 0.47492002783964793 1104 76-77 0.5166382042253765 1104 78-79 0.5116581462185152 1104 80-81 0.7090370171912568 1104 82-83 0.6763415008104943 1104 84-85 0.6607455891363578 1104 86-87 0.6805945106452604 1104 88-89 0.5730737035723656 1104 90-91 0.7469975783044411 1104 92-93 0.6183911612759978 1104 94-95 0.6196818497131922 1104 96-97 0.5881054370025893 1104 98-99 0.4204788752991284 1104 100-101 0.5199187706263615 1105 1 0.24047174016683925 1105 2 0.19818097218330877 1105 3 0.17496354604061537 1105 4 0.15140115890618944 1105 5 0.14457091728674243 1105 6 0.16823975222617804 1105 7 0.17633757122592897 1105 8 0.13561758018747128 1105 9 0.2125378204267605 1105 10-11 0.14605842993800167 1105 12-13 0.22221397794124442 1105 14-15 0.2923676913310942 1105 16-17 0.19948445711955287 1105 18-19 0.11297843077540648 1105 20-21 0.1961134449249542 1105 22-23 0.25179616255649506 1105 24-25 0.21447057485006837 1105 26-27 0.24911412246516562 1105 28-29 0.2226903333761001 1105 30-31 0.2657072496096049 1105 32-33 0.23075333580358404 1105 34-35 0.24997352201199874 1105 36-37 0.26472233665992206 1105 38-39 0.4194985873952177 1105 40-41 0.33932332377484187 1105 42-43 0.445119190850896 1105 44-45 0.4877429033098224 1105 46-47 0.42028378638127606 1105 48-49 0.4046996081385501 1105 50-51 0.4096430219044862 1105 52-53 0.4064507488141871 1105 54-55 0.44072492210490566 1105 56-57 0.33980433158119183 1105 58-59 0.21915259398296172 1105 60-61 0.24166297687649063 1105 62-63 0.39036684459575355 1105 64-65 0.1179846690063826 1105 66-67 0.2342924813809404 1105 68-69 0.25367616520576775 1105 70-71 0.16830084992719918 1105 72-73 0.24346806045671343 1105 74-75 0.35996582891028694 1105 76-77 0.1364960726822524 1105 78-79 0.3353939502840788 1105 80-81 0.28542972479163353 1105 82-83 0.3406164754373364 1105 84-85 0.3070080002922495 1105 86-87 0.12717930456042126 1105 88-89 0.07132812166035407 1105 90-91 0.06385418371654694 1105 92-93 0.06637980338679839 1105 94-95 0.0950436288900427 1105 96-97 0.026276655488782552 1105 98-99 0.24662983864190835 1105 100-101 0.5370582577289582 1106 1 0.23916398079456513 1106 2 0.1872829774143483 1106 3 0.15970635336406502 1106 4 0.13832356518343403 1106 5 0.12103124858578695 1106 6 0.17259895013376791 1106 7 0.2282120263261902 1106 8 0.2058006664995915 1106 9 0.1994602267040122 1106 10-11 0.16414910125447335 1106 12-13 0.24270220810689835 1106 14-15 0.3226641167888076 1106 16-17 0.2648724257333228 1106 18-19 0.08682324332989566 1106 20-21 0.177586853817715 1106 22-23 0.13867497685466645 1106 24-25 0.2547931554952285 1106 26-27 0.3210408879403204 1106 28-29 0.38267289658445947 1106 30-31 0.35049364891212775 1106 32-33 0.3595676339727234 1106 34-35 0.3909935743223727 1106 36-37 0.3665096078020298 1106 38-39 0.47180896228623936 1106 40-41 0.3787740648384812 1106 42-43 0.29123950471314686 1106 44-45 0.24580741943885442 1106 46-47 0.2158374045155398 1106 48-49 0.3591459900042864 1106 50-51 0.32616438197423747 1106 52-53 0.3443321786310989 1106 54-55 0.3291294556707314 1106 56-57 0.3369708529412634 1106 58-59 0.438638208629861 1106 60-61 0.3443220876001192 1106 62-63 0.5738890765050826 1106 64-65 0.3980631345687158 1106 66-67 0.45552177519088133 1106 68-69 0.47795689755101733 1106 70-71 0.4268012858469916 1106 72-73 0.378167275801097 1106 74-75 0.4510730651788073 1106 76-77 0.22476983031084785 1106 78-79 0.591278867459323 1106 80-81 0.4835552696913723 1106 82-83 0.6669024388200775 1106 84-85 0.5502512435354951 1106 86-87 0.3804487029911101 1106 88-89 0.3202383221834566 1106 90-91 0.3537408445709467 1106 92-93 0.3660746595332718 1106 94-95 0.32215783987522073 1106 96-97 0.35844753604676427 1106 98-99 0.14244500865062548 1106 100-101 0.2811733405537211 1107 1 0.04361698422674465 1107 2 0.05550387287085101 1107 3 0.03585032093310758 1107 4 0.061932392467234365 1107 5 0.08412815521066364 1107 6 0.08869671388363543 1107 7 0.11045408872341511 1107 8 0.0987626771014547 1107 9 0.01622676866450945 1107 10-11 0.10219220765200987 1107 12-13 0.07965864187836047 1107 14-15 0.06462608692554284 1107 16-17 0.020428287842882753 1107 18-19 0.06385928304458588 1107 20-21 0.03825585274722698 1107 22-23 0.04650001299738449 1107 24-25 0.054767221026665425 1107 26-27 0.08187266704486262 1107 28-29 0.11833113546850882 1107 30-31 0.054793436654144045 1107 32-33 0.05520199843109452 1107 34-35 0.03075608080598613 1107 36-37 0.0489276935071743 1107 38-39 0.15667279712965865 1107 40-41 0.11949228407854662 1107 42-43 0.08975958461337541 1107 44-45 0.045938195376081126 1107 46-47 -0.02023964844595838 1107 48-49 0.0052992277663506115 1107 50-51 -0.07472511511779345 1107 52-53 0.06408357559768518 1107 54-55 0.025726706249201925 1107 56-57 0.12568715192706037 1107 58-59 0.15585078666318708 1107 60-61 0.11883052825800888 1107 62-63 0.1905951047056007 1107 64-65 0.1116825931452965 1107 66-67 0.12533350198505389 1107 68-69 -0.030952052231754124 1107 70-71 0.10746263318861793 1107 72-73 -0.04675905927224022 1107 74-75 0.11863456297713881 1107 76-77 0.10381992981827892 1107 78-79 0.2996110052320482 1107 80-81 0.41871983401069457 1107 82-83 0.30122146589124554 1107 84-85 0.3082212184103277 1107 86-87 0.04336186114722196 1107 88-89 0.15052168844068703 1107 90-91 0.26431898089428785 1107 92-93 0.26834751221445075 1107 94-95 0.5150986982509487 1107 96-97 0.45674358628237854 1107 98-99 0.1771644565591295 1107 100-101 0.3668415117874275 1108 1 0.1694919855642567 1108 2 0.050994847329690174 1108 3 0.05729726088053155 1108 4 0.09085621126446597 1108 5 0.0744884278494169 1108 6 0.1586008765823408 1108 7 0.10420629562070616 1108 8 0.15202924176042387 1108 9 0.1263023470945157 1108 10-11 0.09899180532794816 1108 12-13 0.06547796698686881 1108 14-15 0.048007749080333895 1108 16-17 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-0.8906816248167289 2316 86-87 -0.8854623693715737 2316 88-89 -1.1631967257677225 2316 90-91 -1.1728502626653174 2316 92-93 -1.3645269097779753 2316 94-95 -1.2515126047629686 2316 96-97 -0.8741156723228976 2316 98-99 -1.16618620320639 2316 100-101 -1.0769347675543912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 12.0 5 44.0 6 106.0 7 258.0 8 574.0 9 1104.0 10 1945.0 11 3256.0 12 4983.0 13 6994.0 14 9023.0 15 12348.0 16 16629.0 17 22707.0 18 19977.0 19 12069.0 20 10903.0 21 11083.0 22 11814.0 23 13181.0 24 15630.0 25 18926.0 26 22934.0 27 29157.0 28 36120.0 29 45147.0 30 56710.0 31 71641.0 32 90784.0 33 118761.0 34 161388.0 35 227559.0 36 345239.0 37 506602.0 38 342198.0 39 19750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.631798579938454 20.041297888258377 15.199625575223738 22.12727795657943 2 15.35456771850687 21.459427729188597 39.96885451016418 23.21715004214035 3 19.181948518208348 25.745570457042135 30.416672730455968 24.655808294293553 4 12.657278850423959 17.797542633036816 38.256240548715816 31.28893796782341 5 13.282068266300223 38.13934884221417 34.26924347349421 14.309339417991401 6 30.310320207482842 37.59171197599535 17.935085318542697 14.162882497979107 7 27.480760164776164 33.60341794553708 21.314531340060974 17.601290549625787 8 26.030920447123584 34.75028896686793 20.21396557799327 19.00482500801522 9 26.398916191375836 16.974420511219332 20.810131070459466 35.81653222694536 10-11 24.370350179862143 27.274695980932318 27.772624787632118 20.58232905157342 12-13 25.833902594020625 24.72328197565578 28.28305390681882 21.15976152350477 14-15 22.697316632275825 25.813947232290552 27.076603451902727 24.412132683530896 16-17 21.47282917637056 29.22697559183634 27.439482333661587 21.860712898131517 18-19 21.37210083826082 27.60911326399571 30.268633310283512 20.750152587459958 20-21 22.4410716278118 26.53883656348441 30.175970357316583 20.844121451387206 22-23 21.98165475859192 26.602871256519823 30.062416510805466 21.353057474082792 24-25 21.85593332658519 26.793456771419454 30.151694280022785 21.19891562197257 26-27 21.868436952849702 26.821776195957536 30.131227865909864 21.178558985282898 28-29 21.420857011564003 27.075760930059438 30.223866774506803 21.279515283869756 30-31 21.708276708968 26.648626252286935 30.227830058767324 21.415266979977744 32-33 21.579720556980806 26.848528229425355 30.067225623269252 21.50452559032459 34-35 21.98961791987073 26.823666485708653 29.932623642759776 21.254091951660836 36-37 22.02576821485009 26.535823269898522 29.970490000074978 21.467918515176414 38-39 21.718534671256375 26.96187951415054 29.952182064094956 21.367403750498127 40-41 21.953062070057396 26.831770213049328 29.884104992819267 21.331062724074005 42-43 21.749403874912296 26.959318845226214 29.532634403220026 21.758642876641467 44-45 22.00719186826727 26.798639595583097 29.342452088389244 21.851716447760392 46-47 22.197940023827474 27.190199292143657 28.907492909198712 21.704367774830153 48-49 22.136819137523663 27.350839532388893 28.673495826318394 21.838845503769047 50-51 21.956686167667446 27.407057848068604 28.67149891616187 21.96475706810208 52-53 22.305530038662685 27.71259516282032 28.14330022433796 21.83857457417904 54-55 22.19675354902102 27.350642769774442 28.32103229493914 22.131571386265396 56-57 22.204922068823933 27.571228254783996 28.208521216159866 22.0153284602322 58-59 22.499203989490017 27.318485348555583 28.219961738575154 21.962348923379245 60-61 22.359312466235213 27.394317592970307 28.66239622495893 21.583973715835548 62-63 22.209116350372657 27.37485123585872 28.585538306228877 21.830494107539742 64-65 22.078114801813403 27.51894106440403 28.422643722768086 21.980300411014483 66-67 22.165522679523068 27.60043183971126 28.03456481741189 22.19948066335378 68-69 22.17909205710381 27.37691640928586 28.332593549570696 22.111397984039634 70-71 22.272009532820782 27.408248915286627 28.36494880232252 21.954792749570075 72-73 22.348748280641622 27.20447211783939 28.36966679176437 22.077112809754624 74-75 22.032924362343504 27.81563538974255 27.957549984322362 22.193890263591587 76-77 22.110937699140177 27.4918293267524 28.113929459891047 22.283303514216378 78-79 22.12991885859592 27.540161665394354 28.023398219113986 22.30652125689574 80-81 22.210273315619453 27.701590941665472 27.886462111773852 22.201673630941222 82-83 22.180430318898733 27.598813311848335 28.080329252374558 22.140427116878374 84-85 22.066955457116293 27.52149168999748 27.9499582779833 22.461594574902925 86-87 22.013088146863147 27.497192357213752 27.993979196651424 22.495740299271677 88-89 22.056520261770533 27.442864208354496 27.87008582138013 22.630529708494844 90-91 22.08037308989879 27.30402857709863 28.123812043835862 22.491786289166722 92-93 22.164463123357265 27.319365308966464 28.08547513626982 22.430696431406446 94-95 21.988828963990155 27.19069678625995 28.108930279919765 22.711543969830135 96-97 21.886141085279522 27.29176699707344 28.197452886605028 22.62463903104201 98-99 21.717135523140804 27.310161032722746 27.918181996590548 23.0545214475459 100-101 21.418985903765993 27.49078743810517 27.969298222403328 23.120928435725514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 801.0 1 569.0 2 390.5 3 443.5 4 376.5 5 364.5 6 624.0 7 1361.0 8 2309.0 9 2730.0 10 2379.5 11 2038.5 12 2145.0 13 2289.5 14 2466.5 15 2676.0 16 2878.0 17 3143.0 18 3346.5 19 3571.0 20 4084.0 21 4718.5 22 5469.5 23 6487.5 24 7867.5 25 9685.0 26 12503.0 27 15800.5 28 18627.5 29 22118.5 30 26989.5 31 31989.0 32 37523.0 33 43400.0 34 49905.5 35 55409.5 36 61546.0 37 68725.0 38 71975.0 39 72399.5 40 73331.5 41 76012.5 42 78466.0 43 81041.0 44 84398.0 45 86856.0 46 86694.0 47 89141.0 48 96734.0 49 94808.5 50 84134.5 51 77295.0 52 73215.5 53 70131.5 54 65967.5 55 59899.5 56 53973.0 57 48185.0 58 43703.0 59 39205.0 60 33787.0 61 27585.5 62 21412.0 63 16457.5 64 12164.0 65 8925.5 66 6361.0 67 4764.0 68 3271.0 69 1922.5 70 1210.0 71 831.5 72 589.0 73 434.5 74 331.0 75 252.0 76 177.0 77 80.0 78 34.5 79 19.0 80 11.0 81 8.5 82 5.0 83 3.0 84 2.0 85 1.5 86 1.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027209852347963297 2 0.006085834074584984 3 8.820049383456499E-5 4 5.292029630073899E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 8.820049383456499E-5 10-11 0.01186296642074899 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.969022222555424E-4 20-21 8.820049383456499E-4 22-23 0.0022932128396986893 24-25 0.011929116791124914 26-27 0.005777132346164006 28-29 3.0870172842097744E-4 30-31 0.0026239646915783084 32-33 0.005380230123908464 34-35 0.0 36-37 0.008555447901952803 38-39 0.013913627902402626 40-41 0.001521458518646246 42-43 0.0 44-45 0.0 46-47 4.6305259263146613E-4 48-49 0.0034618693830066757 50-51 0.006879638519096068 52-53 0.0 54-55 0.003064967160751133 56-57 0.003505969629923958 58-59 1.3230074075184747E-4 60-61 0.0015876088890221696 62-63 0.005710981975788083 64-65 4.410024691728249E-5 66-67 0.002337313086615972 68-69 2.8665160496233616E-4 70-71 0.001168656543307986 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0016758093828567347 80-81 0.0015876088890221696 82-83 0.010694309877441003 84-85 0.0076293427166898705 86-87 0.003946972099096783 88-89 1.1025061729320624E-4 90-91 4.8510271609010735E-4 92-93 4.410024691728249E-5 94-95 2.2050123458641246E-5 96-97 0.002337313086615972 98-99 0.0017860600001499407 100-101 2.2050123458641248E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2267561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.303623045078375 #Duplication Level Percentage of deduplicated Percentage of total 1 73.43342136863409 38.40833990175441 2 14.613524478486353 15.286805513655516 3 5.09476542903661 7.994240715102836 4 2.331800797584654 4.8784651973232345 5 1.2430009351477314 3.2506726178323433 6 0.7430849284700215 2.3319620393505023 7 0.483441175272012 1.7700007497127888 8 0.3442354459351699 1.440380880235807 9 0.26096420849992397 1.2284436230673534 >10 1.300979252235266 12.446452180140689 >50 0.09695457426768397 3.5187148950315756 >100 0.05052945899083007 4.747042706740535 >500 0.002029506116620364 0.6845381844958424 >1k 9.301903034508386E-4 0.7312886439524905 >5k 2.5368826457750147E-4 0.8177128613398841 >10k+ 8.456275485916715E-5 0.46493929026421804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10512 0.46358179559447354 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8051 0.35505108793104134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5331 0.23509841631603295 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5106 0.2251758607596444 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2050123458641246E-5 0.0 16-17 0.0 0.0 0.0 6.615037037592373E-5 0.0 18-19 0.0 0.0 0.0 1.1025061729320623E-4 0.0 20-21 0.0 0.0 0.0 1.5435086421048872E-4 0.0 22-23 0.0 0.0 0.0 3.3075185187961866E-4 0.0 24-25 0.0 0.0 0.0 7.27654074135161E-4 0.0 26-27 0.0 0.0 0.0 0.0010363558025561386 0.0 28-29 0.0 0.0 0.0 0.002160912098946842 0.0 30-31 0.0 0.0 0.0 0.004586425679397379 0.0 32-33 0.0 0.0 0.0 0.007585242469772588 0.0 34-35 0.0 0.0 0.0 0.010209207161350896 0.0 36-37 0.0 0.0 0.0 0.012259868643004532 0.0 38-39 0.0 0.0 0.0 0.01450898123578594 0.0 40-41 0.0 0.0 0.0 0.016713993581650065 0.0 42-43 0.0 0.0 0.0 0.01997741185352897 0.0 44-45 0.0 0.0 0.0 0.023306980495783794 0.0 46-47 0.0 0.0 0.0 0.02767290494059476 0.0 48-49 0.0 0.0 0.0 0.032832633829916816 0.0 50-51 0.0 0.0 0.0 0.03971227234901288 0.0 52-53 0.0 0.0 0.0 0.056889318523294416 0.0 54-55 0.0 0.0 0.0 0.07638162766073328 0.0 56-57 0.0 0.0 0.0 0.1033930288975688 0.0 58-59 0.0 0.0 0.0 0.14050338667846202 0.0 60-61 0.0 0.0 0.0 0.19472464026326083 0.0 62-63 0.0 0.0 0.0 0.23507636619257433 0.0 64-65 0.0 0.0 0.0 0.27578089409722606 0.0 66-67 0.0 0.0 0.0 0.3531768274370568 0.0 68-69 0.0 0.0 0.0 0.45390179139613 0.0 70-71 0.0 0.0 0.0 0.5275492037479919 0.0 72-73 0.0 0.0 0.0 0.5937216242473742 0.0 74-75 0.0 0.0 0.0 0.732086148950348 0.0 76-77 0.0 0.0 0.0 0.8339577193292705 0.0 78-79 0.0 0.0 0.0 0.9091486403232372 0.0 80-81 0.0 0.0 0.0 1.0008110035408087 0.0 82-83 0.0 0.0 0.0 1.1720081620736993 0.0 84-85 0.0 0.0 0.0 1.347328693693356 0.0 86-87 0.0 0.0 0.0 1.5856464280343505 0.0 88-89 0.0 0.0 0.0 1.8884166732449534 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16740 0.0 26.818945 1 GTACATG 11205 0.0 23.573725 1 GGTATCA 12185 0.0 23.042374 1 TACATGG 11555 0.0 22.5253 2 TATCAAC 20295 0.0 22.139204 2 ATCAACG 20150 0.0 22.133032 3 TCAACGC 20150 0.0 22.133032 4 GAGTACT 9590 0.0 22.113283 12-13 CAACGCA 20280 0.0 21.95109 5 AACGCAG 20770 0.0 21.478975 6 ACATGGG 11475 0.0 21.067617 3 GTACTTT 10335 0.0 20.358398 14-15 AGTACTT 10150 0.0 20.284927 12-13 GTATAGG 700 0.0 19.68182 1 AGAGTAC 16625 0.0 19.61839 10-11 ACGCAGA 22770 0.0 19.321125 7 CGCAGAG 22965 0.0 19.136377 8 GCAGAGT 23800 0.0 18.105677 9 TACTTTT 11570 0.0 17.672182 14-15 CATGGGA 7410 0.0 17.498259 4 >>END_MODULE