FastQCFastQC Report
Thu 26 May 2016
SRR936896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264972
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.3970910015664653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.27881139369493313No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44080.19461609238436503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42020.18552105721395232No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24720.10914042204495243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152250.027.9133821
GTACATG113850.026.8478891
TACATGG117350.025.1176622
ACATGGG118050.023.7202873
TCAACGC178400.023.5307874
GGTATCA109600.023.4649121
ATCAACG179500.023.4130733
CAACGCA180300.023.2823075
TATCAAC181000.023.2190422
AACGCAG184350.022.8173486
GAGTACT73900.022.6365612-13
GTACTTT79550.020.93942814-15
CATGGGA75250.020.5959434
ACGCAGA202550.020.4376877
CGCAGAG202700.020.1411958
AGTACTT79950.020.12113612-13
AGAGTAC144650.019.82742310-11
GCAGAGT204450.019.270129
GCGCAAC650.00758163418.26289230-31
CATGGGG47000.018.2072874
Produced by FastQC (version 0.11.5)