FastQCFastQC Report
Thu 26 May 2016
SRR936896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264972
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106190.4688358178379247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79330.35024715537322315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.23898750183225223No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49000.21633821521855456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161450.027.8652061
CGTACCG700.00839333827.1428055
GTACATG103500.024.2809491
GGTATCA116350.023.804141
TATAACG1401.780497E-523.7499542
TCAACGC192300.023.2682864
ATCAACG192900.023.1959133
CAACGCA194300.023.028785
TATCAAC195900.022.9861742
TACATGG107600.022.5580442
AACGCAG200050.022.3920966
GAGTACT95050.022.3896612-13
ACATGGG104150.022.0738933
AGTACTT100650.021.23832712-13
GTACTTT102550.020.70426814-15
AGAGTAC162450.020.3096710-11
ACGCAGA221550.020.1118817
CGCAGAG221350.020.0026138
GCAGAGT228250.018.9400429
TACTTTT114900.018.49954214-15
Produced by FastQC (version 0.11.5)