FastQCFastQC Report
Thu 26 May 2016
SRR936895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109453
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.5499106316355898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.38352233037361655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.24633760961482823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.044.8596461
GTATCAA58650.041.503241
GTATCGA550.00256835434.5762021
ATCAACG73900.032.549693
TATCAAC74400.032.4006922
TCAACGC74850.032.2000774
CAACGCA75550.031.8930915
AACGCAG77450.031.2334186
ACGCAGA87650.027.3818367
CGCAGAG87050.027.0792128
GCAGAGT92800.024.8380159
GTACATG59500.024.0508021
GAGTACT46250.023.88506712-13
TACATGG60300.023.1030642
ACATGGG58850.023.0217933
CGTATAG1050.002184843622.6391831
AGTACTT49300.022.2146412-13
GGACCGT1100.002868491721.6023226
GTACTTT51950.021.26581814-15
AGAGTAC83400.020.82314710-11
Produced by FastQC (version 0.11.5)