FastQCFastQC Report
Thu 26 May 2016
SRR936895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109453
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.6361693555292562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.49826355870866096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.32196046159684094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.1281712699862004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.045.8114851
GTATCAA63650.038.8107761
ATCAACG83550.029.4464443
TCAACGC84800.028.9563754
CAACGCA85600.028.630275
TATCAAC86700.028.486152
AACGCAG88550.027.6764686
ACGCAGA99700.024.4848757
AGGACCG1951.571425E-824.3565035
CGCAGAG100100.024.24478
GTATAGG3000.023.7529411
GCAGAGT105150.022.8093059
GAGTACT62750.021.83544312-13
AGTACTT65500.021.60751712-13
GTATAGA3600.021.1137261
GTACATG54350.020.8029441
AGAGTAC97400.020.57706510-11
GTACTTT65800.020.57347314-15
TACATGG54800.020.2807882
ACATGGG54050.019.419863
Produced by FastQC (version 0.11.5)