FastQCFastQC Report
Thu 26 May 2016
SRR936894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891593
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.5693180632867239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.4204833371280393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.2624515894584188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.10296177740291815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.043.8651731
GTATCAA51750.042.424191
ATCAACG63950.034.2564473
TCAACGC64600.033.915574
CAACGCA65350.033.4554865
TATCAAC65250.033.4282882
AACGCAG67150.032.700256
ACGCAGA75150.028.648377
CGCAGAG75150.028.5218898
CGCGAAA350.00839686527.14015660-61
GCAGAGT80050.026.241689
GTATAGG2501.4551915E-1124.7107121
GAGTACT43650.023.46354912-13
GTACATG46500.023.0960351
GTACTTT45350.022.48045714-15
TACATGG47650.022.1397152
ACATGGG46300.021.861533
GTGTAGC5250.021.7237051
AGTACTT45150.021.3156212-13
AGAGTAC73700.020.86228610-11
Produced by FastQC (version 0.11.5)