##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936894_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891593 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92715398169344 33.0 31.0 34.0 30.0 34.0 2 32.03557116307553 33.0 31.0 34.0 30.0 34.0 3 32.04953605512829 34.0 31.0 34.0 30.0 34.0 4 35.636170315379324 37.0 35.0 37.0 33.0 37.0 5 35.651823197355746 37.0 35.0 37.0 33.0 37.0 6 35.727424957351616 37.0 35.0 37.0 33.0 37.0 7 35.67238863472459 37.0 35.0 37.0 33.0 37.0 8 35.62659195395209 37.0 35.0 37.0 33.0 37.0 9 37.289459428236874 39.0 37.0 39.0 34.0 39.0 10-11 37.12472058439221 39.0 37.0 39.0 33.5 39.0 12-13 37.069652857301485 39.0 37.0 39.0 33.0 39.0 14-15 38.24975016627542 40.0 38.0 41.0 33.0 41.0 16-17 38.19307239962629 40.0 38.0 41.0 33.0 41.0 18-19 38.198867083972175 40.0 38.0 41.0 33.5 41.0 20-21 38.15202676557577 40.0 38.0 41.0 33.5 41.0 22-23 38.04210160914229 40.0 38.0 41.0 33.0 41.0 24-25 37.896564912465664 40.0 38.0 41.0 33.0 41.0 26-27 37.97962242861934 40.0 38.0 41.0 33.0 41.0 28-29 37.926594309286855 40.0 38.0 41.0 33.0 41.0 30-31 37.77010138033834 40.0 37.5 41.0 33.0 41.0 32-33 37.56040143877307 40.0 37.0 41.0 33.0 41.0 34-35 37.40032896175721 40.0 37.0 41.0 32.0 41.0 36-37 37.232200118215374 40.0 36.0 41.0 31.5 41.0 38-39 37.08131232524257 40.0 36.0 41.0 31.0 41.0 40-41 36.90497065365027 39.0 36.0 41.0 31.0 41.0 42-43 36.6799464553894 39.0 36.0 41.0 30.5 41.0 44-45 36.391752178404275 39.0 35.5 41.0 30.0 41.0 46-47 35.99393052659678 39.0 35.0 41.0 29.5 41.0 48-49 35.63154881207008 39.0 35.0 40.0 27.5 41.0 50-51 34.97039568502669 38.5 34.5 40.0 26.5 40.5 52-53 35.1885378193862 38.5 35.0 40.0 27.5 40.5 54-55 35.57861714930468 39.0 35.0 41.0 28.0 41.0 56-57 35.49301923635561 39.0 35.0 41.0 27.0 41.0 58-59 35.27412339486739 39.0 35.0 41.0 26.5 41.0 60-61 35.020266534169735 38.5 35.0 40.5 26.0 41.0 62-63 34.72622934455519 38.0 34.5 40.0 26.0 41.0 64-65 34.4199399277473 38.0 34.0 40.0 25.5 41.0 66-67 34.06251002419265 37.0 34.0 40.0 24.5 41.0 68-69 33.70007952058843 37.0 34.0 39.5 23.5 41.0 70-71 33.30398455349021 36.0 33.5 39.0 22.5 41.0 72-73 32.91549114898838 36.0 33.0 39.0 22.0 40.0 74-75 32.49211467564236 35.0 33.0 38.0 20.5 40.0 76-77 32.061302073928346 35.0 32.0 37.0 20.0 39.0 78-79 31.623647785480593 35.0 32.0 37.0 18.0 39.0 80-81 31.204270334109843 35.0 31.5 36.0 17.0 38.5 82-83 30.800433605916602 35.0 31.0 36.0 12.0 37.0 84-85 30.44471524563338 35.0 31.0 35.0 7.5 37.0 86-87 30.115887518183747 34.0 31.0 35.0 6.5 36.5 88-89 29.77918007431642 34.0 30.5 35.0 2.0 36.0 90-91 29.450523389035133 34.0 30.0 35.0 2.0 36.0 92-93 29.105646859048917 34.0 30.0 35.0 2.0 35.5 94-95 28.72613905672207 34.0 29.0 35.0 2.0 35.0 96-97 28.29748102553519 34.0 29.0 35.0 2.0 35.0 98-99 27.750009813894906 34.0 29.0 35.0 2.0 35.0 100-101 25.632674886411177 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4716172556927596 1101 2 0.507288236426156 1101 3 0.43015857078555797 1101 4 0.3604817879018185 1101 5 0.4748067470047914 1101 6 0.25468801008227615 1101 7 0.3329877496809175 1101 8 0.44593671827254866 1101 9 0.4326220767693272 1101 10-11 0.5515853703912583 1101 12-13 0.4280106719986847 1101 14-15 0.860931721921979 1101 16-17 0.8439996387910398 1101 18-19 0.751560348166251 1101 20-21 0.6600372904553069 1101 22-23 0.7546495116191139 1101 24-25 0.7833259366617895 1101 26-27 0.7934195040409975 1101 28-29 0.9147518500414051 1101 30-31 0.8034757632219893 1101 32-33 0.9932176733583375 1101 34-35 0.9092897393709833 1101 36-37 0.804091092971035 1101 38-39 0.9006798511276557 1101 40-41 0.8504540430747056 1101 42-43 0.91393661672776 1101 44-45 1.080149847985652 1101 46-47 0.8526195056555181 1101 48-49 0.945391142880716 1101 50-51 0.8676306804606995 1101 52-53 0.93976286004294 1101 54-55 0.8330518835769993 1101 56-57 0.9913412226897691 1101 58-59 0.9754442640971135 1101 60-61 1.1240181941522636 1101 62-63 1.0462510308401605 1101 64-65 1.0475205323206254 1101 66-67 1.032429998602531 1101 68-69 1.0715954092382702 1101 70-71 1.2354371003317297 1101 72-73 1.2590541329758054 1101 74-75 1.3305798479963968 1101 76-77 1.1151402894318316 1101 78-79 1.257641456887999 1101 80-81 1.1029507302464587 1101 82-83 1.2386796895752354 1101 84-85 1.1565806034253683 1101 86-87 1.1637836328664228 1101 88-89 1.137660626511746 1101 90-91 1.1715467797997086 1101 92-93 1.206239579824988 1101 94-95 1.3702608232126288 1101 96-97 1.2592791444315061 1101 98-99 1.3366152227102006 1101 100-101 1.4917193843406267 1102 1 0.4918176215144321 1102 2 0.3095973568850283 1102 3 0.34928437839359816 1102 4 0.3288205749635935 1102 5 0.25324812219191273 1102 6 0.198360749724678 1102 7 0.2666003933208998 1102 8 0.2195121154479054 1102 9 0.28122814042962574 1102 10-11 0.48737902818420764 1102 12-13 0.48877984814399866 1102 14-15 0.3493463190542698 1102 16-17 0.5293262841009252 1102 18-19 0.429084363087739 1102 20-21 0.2391061402931811 1102 22-23 0.32711232528486534 1102 24-25 0.4654788441652542 1102 26-27 0.4399540480753146 1102 28-29 0.46770808773616324 1102 30-31 0.4748510190623634 1102 32-33 0.3883151017258939 1102 34-35 0.4825568682206196 1102 36-37 0.45772789183668294 1102 38-39 0.37922132905179495 1102 40-41 0.23448318969313675 1102 42-43 0.45294318296335945 1102 44-45 0.6141226438642562 1102 46-47 0.5147530110953156 1102 48-49 0.5991138422644084 1102 50-51 0.5663474985222621 1102 52-53 0.5776887404946152 1102 54-55 0.6817756309470084 1102 56-57 0.7966373904958601 1102 58-59 0.7988104648775405 1102 60-61 0.6330013839935518 1102 62-63 0.7883473241755397 1102 64-65 0.8162256564583359 1102 66-67 0.49640647102331314 1102 68-69 0.2853373732448148 1102 70-71 0.6659759371012726 1102 72-73 0.7808934806816339 1102 74-75 0.7137806272858924 1102 76-77 0.5809178093807894 1102 78-79 0.4188131628608325 1102 80-81 0.4048100183258718 1102 82-83 0.5371894301213125 1102 84-85 0.5974097495856725 1102 86-87 0.3582624057030763 1102 88-89 0.5533756108835775 1102 90-91 0.5797053819348505 1102 92-93 0.6607263356831155 1102 94-95 0.6161015201292557 1102 96-97 0.6688934740123216 1102 98-99 0.7206973413147608 1102 100-101 0.5970294107636249 1103 1 0.28027922867748956 1103 2 0.2900040955331775 1103 3 0.20043048921427697 1103 4 0.23853747981537765 1103 5 0.3223988129307358 1103 6 0.14688906697987392 1103 7 0.14483958984654777 1103 8 0.3387888551558902 1103 9 0.24907880604201438 1103 10-11 0.4577033378323989 1103 12-13 0.38223453065182866 1103 14-15 0.41884264714122565 1103 16-17 0.5032915453146458 1103 18-19 0.37933312671228236 1103 20-21 0.4634878930228439 1103 22-23 0.4656655665355558 1103 24-25 0.49580889359645397 1103 26-27 0.33555281342655263 1103 28-29 0.46932910010527706 1103 30-31 0.393444641234602 1103 32-33 0.6285473485889597 1103 34-35 0.49032492620909096 1103 36-37 0.2990053269872561 1103 38-39 0.3853910003718113 1103 40-41 0.35395701116207334 1103 42-43 0.44882977104342103 1103 44-45 0.5959247332881716 1103 46-47 0.46932149730495354 1103 48-49 0.44846329382932737 1103 50-51 0.5446152153389932 1103 52-53 0.6995037265679045 1103 54-55 0.6143108526133787 1103 56-57 0.8193553801904514 1103 58-59 0.7353799085115682 1103 60-61 0.7674120380787386 1103 62-63 0.7639532463931431 1103 64-65 0.7620516910184918 1103 66-67 0.8617475296441199 1103 68-69 0.8088761400012245 1103 70-71 0.5204429649058753 1103 72-73 0.5296916391877673 1103 74-75 0.49231163580864035 1103 76-77 0.5828174751158421 1103 78-79 0.8017192078526314 1103 80-81 0.8929740975253502 1103 82-83 0.8463651639382768 1103 84-85 0.6485328179164753 1103 86-87 0.6638873930505476 1103 88-89 0.6200851076046376 1103 90-91 0.6799128238850436 1103 92-93 0.5949840876649475 1103 94-95 0.7293529762121764 1103 96-97 0.7308033451291855 1103 98-99 0.8388145138643068 1103 100-101 0.777289558688075 1104 1 0.2925444902229337 1104 2 0.19046718982569644 1104 3 0.20364023617899107 1104 4 0.2562180369468905 1104 5 0.1492242412752276 1104 6 0.3301368259722679 1104 7 0.3434155571675461 1104 8 0.21195562583557148 1104 9 0.28995591939728627 1104 10-11 0.4451778073660435 1104 12-13 0.3455646253982181 1104 14-15 0.47853819632992156 1104 16-17 0.6118687182409985 1104 18-19 0.40531321294852773 1104 20-21 0.5254904035187096 1104 22-23 0.4516223916802389 1104 24-25 0.5246122239039508 1104 26-27 0.3264313451410672 1104 28-29 0.4788895505761701 1104 30-31 0.5611844148644636 1104 32-33 0.5740093081940856 1104 34-35 0.45596659193018496 1104 36-37 0.5250971090138137 1104 38-39 0.5745816845883098 1104 40-41 0.5522493066346641 1104 42-43 0.491130562487875 1104 44-45 0.6021346646395855 1104 46-47 0.5712954987800458 1104 48-49 0.5642843819945469 1104 50-51 0.499642235082554 1104 52-53 0.25716641237296045 1104 54-55 0.4264867575265825 1104 56-57 0.5703375939655615 1104 58-59 0.6112468805982516 1104 60-61 0.7405933469406492 1104 62-63 0.6796878146445948 1104 64-65 0.44017952773698 1104 66-67 0.5177145669753358 1104 68-69 0.6009737514842612 1104 70-71 0.6809537160689345 1104 72-73 0.664401726558701 1104 74-75 0.7018341451698049 1104 76-77 0.5862608967656691 1104 78-79 0.5409779733127529 1104 80-81 0.5863230564496682 1104 82-83 0.6480523022566693 1104 84-85 0.6147595568249073 1104 86-87 0.5773316657159278 1104 88-89 0.621283621164153 1104 90-91 0.7711576468738102 1104 92-93 0.6031885867004618 1104 94-95 0.8055525535411228 1104 96-97 0.8460319137210384 1104 98-99 0.7242727857354083 1104 100-101 0.7757052514147276 1105 1 0.13558701064133416 1105 2 0.26530330531502244 1105 3 0.2805692374685762 1105 4 0.16937447053336285 1105 5 0.18788486600656285 1105 6 0.2519540633988342 1105 7 0.22919432203357815 1105 8 0.3527664511687121 1105 9 0.35303802285050523 1105 10-11 0.32573555202860405 1105 12-13 0.27751449285774044 1105 14-15 0.33477073322632833 1105 16-17 0.3023172961174865 1105 18-19 0.4429779930029767 1105 20-21 0.5004550295396228 1105 22-23 0.35576875935468166 1105 24-25 0.41074831073587603 1105 26-27 0.5323222320876226 1105 28-29 0.5075979973450515 1105 30-31 0.19321488126366404 1105 32-33 0.120872218353135 1105 34-35 0.21587488824771128 1105 36-37 0.16656392016153632 1105 38-39 -0.020956716328726088 1105 40-41 0.31659233572193557 1105 42-43 0.32599900710744834 1105 44-45 0.3553053373720658 1105 46-47 0.14055282432811822 1105 48-49 0.43130419142282506 1105 50-51 0.2954730310418583 1105 52-53 0.10335945578892591 1105 54-55 0.1130473321074632 1105 56-57 0.31434192839742536 1105 58-59 0.26338409581533284 1105 60-61 0.2441034307635519 1105 62-63 0.4685212793243423 1105 64-65 0.6618145220054927 1105 66-67 0.5008264024818558 1105 68-69 0.5149809516562058 1105 70-71 0.4672241344669814 1105 72-73 0.5808510746028261 1105 74-75 0.6591162593695472 1105 76-77 0.4706135081713221 1105 78-79 0.6220766264149127 1105 80-81 0.6352175076604425 1105 82-83 0.6861764962672758 1105 84-85 0.4910392888782127 1105 86-87 0.3169984338775542 1105 88-89 0.31752412840014443 1105 90-91 0.42252469444509444 1105 92-93 0.22816440701856067 1105 94-95 0.5241077727702361 1105 96-97 0.6892987678548685 1105 98-99 0.5953494084607271 1105 100-101 0.5335699168395749 1106 1 0.04890271533115609 1106 2 0.12389475364119562 1106 3 0.08248595942126258 1106 4 0.06293079258205836 1106 5 0.044329854053252404 1106 6 0.166808344109306 1106 7 0.25128986676599396 1106 8 0.18657403903156933 1106 9 0.15445062009888488 1106 10-11 0.2719299345492061 1106 12-13 0.26808337819715433 1106 14-15 0.32534695509988865 1106 16-17 0.32705242103246235 1106 18-19 0.2095918366237015 1106 20-21 0.19695676043765076 1106 22-23 0.19838123628390747 1106 24-25 0.21472921897784403 1106 26-27 0.0966825020301556 1106 28-29 0.21416440503205791 1106 30-31 0.3592610867557866 1106 32-33 0.24617026505332262 1106 34-35 0.4475175696880669 1106 36-37 0.2357138988331826 1106 38-39 0.21314527616914347 1106 40-41 0.19911451039642714 1106 42-43 -0.047954095670057484 1106 44-45 0.27891524756240926 1106 46-47 0.19787735801276796 1106 48-49 0.034157683979913145 1106 50-51 0.27475465857859405 1106 52-53 0.2861754062526458 1106 54-55 0.3928632689461935 1106 56-57 0.1474619782020099 1106 58-59 0.4351968171559477 1106 60-61 0.4888575468212295 1106 62-63 0.48384048668519597 1106 64-65 0.18150892391550855 1106 66-67 0.31203274879351994 1106 68-69 0.23757716244112004 1106 70-71 0.18575014090846054 1106 72-73 0.1834194904040416 1106 74-75 0.046511988877824706 1106 76-77 0.33839134534008153 1106 78-79 0.25026107526826635 1106 80-81 0.3874664173990645 1106 82-83 0.450607720975885 1106 84-85 0.43552478297806374 1106 86-87 0.39331306219301254 1106 88-89 0.3243083263440276 1106 90-91 0.12834015545717747 1106 92-93 0.12400605945759224 1106 94-95 0.19530055697447324 1106 96-97 0.5736456433821822 1106 98-99 0.45968012265136693 1106 100-101 0.6721282249928748 1107 1 0.22061988704833269 1107 2 0.3253002897323256 1107 3 0.26486417019704334 1107 4 0.2940895744507799 1107 5 0.3757065008457232 1107 6 0.12457737997164742 1107 7 0.18824904611868476 1107 8 0.18823363704004947 1107 9 0.16818498880282817 1107 10-11 0.4222669763669771 1107 12-13 0.5728979198271063 1107 14-15 0.5242596793675816 1107 16-17 0.37629740638292475 1107 18-19 0.42960126150128275 1107 20-21 0.6017654968893851 1107 22-23 0.5343371442479281 1107 24-25 0.49742773899887993 1107 26-27 0.46777510486717944 1107 28-29 0.4629367805792768 1107 30-31 0.6501073451476955 1107 32-33 0.7148103508333676 1107 34-35 0.6906538001468121 1107 36-37 0.7483162577844311 1107 38-39 0.36314766653664776 1107 40-41 0.5323146661117946 1107 42-43 0.7692049867020074 1107 44-45 0.6038532687478977 1107 46-47 0.7546298047246438 1107 48-49 0.9203437501862624 1107 50-51 0.4662145584563504 1107 52-53 0.5513832657809985 1107 54-55 0.8636307818029607 1107 56-57 0.9352127106789112 1107 58-59 0.949632929462247 1107 60-61 0.8877620755432005 1107 62-63 0.8986051037824652 1107 64-65 0.758545346286617 1107 66-67 0.7603888589143324 1107 68-69 0.6484352360781003 1107 70-71 0.54203953928711 1107 72-73 0.7938134912645793 1107 74-75 0.8050797489668824 1107 76-77 0.8764579478083085 1107 78-79 0.8625349294285236 1107 80-81 0.7987462201595967 1107 82-83 0.9332160899939872 1107 84-85 0.895694430774487 1107 86-87 0.6985783588376222 1107 88-89 0.36441698562104463 1107 90-91 0.5758135370076403 1107 92-93 0.4988293430029813 1107 94-95 0.5301722625358352 1107 96-97 0.7974762414521273 1107 98-99 0.7332850017329164 1107 100-101 0.4591669694352696 1108 1 0.12517142653962665 1108 2 0.20332218121984624 1108 3 0.17904901414800634 1108 4 0.26814789925325755 1108 5 0.22424575590517293 1108 6 -0.11213942187627168 1108 7 0.1397150408164265 1108 8 0.2147632850438299 1108 9 0.11327777826131324 1108 10-11 0.11288434781355505 1108 12-13 0.15752138099202995 1108 14-15 0.20646956466038802 1108 16-17 0.34592099879177596 1108 18-19 0.13954298919637864 1108 20-21 0.0371029250333379 1108 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2316 58-59 -1.151139874418213 2316 60-61 -1.284227894448982 2316 62-63 -1.2881113018573487 2316 64-65 -1.6096360981989264 2316 66-67 -1.4342508418297015 2316 68-69 -1.463384963699923 2316 70-71 -1.18543404972921 2316 72-73 -1.5186171413658336 2316 74-75 -1.9549502588433683 2316 76-77 -2.2945351873912614 2316 78-79 -2.394185997936127 2316 80-81 -2.317133255320325 2316 82-83 -2.1355449574019083 2316 84-85 -2.3677782538968657 2316 86-87 -2.6202546250897036 2316 88-89 -2.767062440530964 2316 90-91 -2.6774749901432635 2316 92-93 -2.5497894755936343 2316 94-95 -3.1791219373244033 2316 96-97 -3.447683457647635 2316 98-99 -3.330985150772527 2316 100-101 -3.62983907323337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1467.0 3 506.0 4 488.0 5 495.0 6 625.0 7 711.0 8 878.0 9 1107.0 10 1352.0 11 1952.0 12 2679.0 13 3481.0 14 3957.0 15 5253.0 16 7091.0 17 9857.0 18 8641.0 19 4910.0 20 4325.0 21 4381.0 22 4815.0 23 5278.0 24 5925.0 25 6940.0 26 7956.0 27 9509.0 28 11414.0 29 14074.0 30 17127.0 31 21737.0 32 27898.0 33 37090.0 34 52147.0 35 75773.0 36 118426.0 37 185253.0 38 188459.0 39 37611.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.367907972513834 19.96292689057149 15.06667324946253 21.602491887452143 2 14.88215321988865 21.213205840837844 41.27678004402844 22.627860895245064 3 18.613792964183705 26.563695475116166 30.591710400254886 24.230801160445242 4 12.154500235073927 17.690687594043595 39.10474422946806 31.050067941414415 5 12.992184308461688 38.07669343724328 34.524167590693 14.406954663602042 6 29.483264253414994 37.8074224716806 18.53373243680955 14.175580838094854 7 26.648731822451776 34.251175360801255 21.798804268896962 17.301288547850007 8 25.57787427565795 34.85338071182213 20.73433664690942 18.83440836561049 9 26.512393206520517 17.070794592871604 21.16766856870923 35.24914363189865 10-11 23.84302791840711 27.714408057597883 28.07666772734951 20.36589629664549 12-13 25.432993358766886 25.346680898682187 28.42239005824127 20.79793568430965 14-15 22.636094629728667 26.174187433395605 27.42038758076002 23.76933035611571 16-17 21.08826169902368 29.57619795758052 27.815733363258893 21.51980698013691 18-19 20.97100789816379 27.740694255161692 30.957469310945946 20.330828535728575 20-21 22.1098193730042 26.79800760977701 30.8518046146828 20.240368402535992 22-23 21.657079966208144 26.950520509031918 30.60255413484656 20.789845389913378 24-25 21.25626083201059 27.158241302140883 30.840648153860823 20.744849711987705 26-27 21.214949339719734 27.29384645153691 30.650521185335585 20.84068302340777 28-29 21.005163457126926 27.327678171801896 30.839737642982218 20.82742072808896 30-31 21.24262332962794 27.166113474811354 30.788519932293358 20.802743263267352 32-33 20.930193430431785 27.30504130516469 30.644450302007215 21.120314962396318 34-35 21.45303665681995 27.26633864218504 30.458828590289272 20.82179611070574 36-37 21.426728701925665 27.135527897919992 30.388004522531897 21.04973887762244 38-39 21.256746873892347 27.391971500486807 30.494636151129978 20.856645474490872 40-41 21.44419456330935 27.26701699877178 30.43099822216739 20.857790215751486 42-43 21.204055617526038 27.40452479856789 30.182484842468792 21.208934741437275 44-45 21.37235097935742 27.6258034196614 29.873443703001247 21.128401897979934 46-47 21.518905705051598 27.70098124711534 29.370733296731217 21.40937975110185 48-49 21.608588319875995 27.92112456159438 29.010591274171894 21.459695844357732 50-51 21.635387597247025 28.172754233105714 28.881805048320665 21.310053121326597 52-53 21.886184862111087 28.17522497310866 28.635041629699252 21.303548535081 54-55 21.57653635796681 28.1888653586443 28.67415795873134 21.560440324657552 56-57 21.676126627166497 28.554328443770586 28.438890562308128 21.330654366754786 58-59 21.72049318793143 28.084892305828564 28.68881420876584 21.505800297474163 60-61 21.734882181699884 28.15551679072037 28.84391669424454 21.2656843333352 62-63 21.61208381404495 28.106668341795917 29.0395620284843 21.241685815674835 64-65 21.620657570847616 28.045498164066863 28.856931818373017 21.47691244671251 66-67 21.47313796491984 28.31905313177272 28.603871920165485 21.603936983141956 68-69 21.557143522260734 28.193433267160785 28.742371879264915 21.507051331313566 70-71 21.543427657963374 28.195305269652522 28.684241523403543 21.577025548980565 72-73 21.507787022713224 28.221857510329034 28.445433628854666 21.82492183810307 74-75 21.42037828217645 28.455466503332588 28.305089213949135 21.819066000541827 76-77 21.546404417048244 28.299646216558994 28.48004379801978 21.673905568372984 78-79 21.42254466139025 28.37695312938283 28.34963231911604 21.85086989011088 80-81 21.29492246116046 28.55645091170193 28.303457483360496 21.845169143777117 82-83 21.537112651998385 28.532305809217313 28.205772586803928 21.724808951980375 84-85 21.51779949978433 28.278563700092718 28.116910688674363 22.08672611144859 86-87 21.486915822845873 28.2219631987184 28.338523775440834 21.9525972029949 88-89 21.63879109051004 28.044891839698284 28.230940907966566 22.08537616182511 90-91 21.369813839483527 28.266334617779197 28.415476170444283 21.94837537229299 92-93 21.505190563753676 28.238058001029927 28.30397081212437 21.952780623092035 94-95 21.36563678972763 28.251538869711833 28.19006260930827 22.192761731252265 96-97 21.42380294142727 28.276006767885754 28.139964634655346 22.16022565603163 98-99 21.229945533885193 28.196636870310947 28.085314348653835 22.488103247150026 100-101 21.329283300122388 28.366206651579112 28.136245870610306 22.168264177688194 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 171.5 2 134.5 3 172.5 4 186.0 5 190.0 6 300.0 7 585.0 8 1020.5 9 1364.5 10 1312.0 11 1157.0 12 1197.5 13 1335.0 14 1435.0 15 1497.5 16 1570.0 17 1655.5 18 1762.5 19 1880.5 20 2076.0 21 2343.0 22 2782.5 23 3332.5 24 3963.0 25 4934.5 26 6358.0 27 7880.5 28 9144.5 29 10605.0 30 12440.5 31 14370.0 32 16576.5 33 18814.5 34 21252.5 35 23607.0 36 26013.5 37 28335.0 38 29381.0 39 29793.5 40 30249.0 41 30973.0 42 31494.0 43 32021.5 44 32997.5 45 33668.0 46 33207.5 47 32649.0 48 34172.5 49 33876.5 50 31280.0 51 29581.5 52 27535.0 53 26108.5 54 24709.0 55 22329.5 56 20197.0 57 17754.0 58 15601.5 59 13632.5 60 11212.0 61 9022.0 62 7000.5 63 5055.0 64 3533.0 65 2586.5 66 1839.5 67 1329.5 68 937.5 69 607.0 70 396.0 71 278.0 72 196.0 73 152.0 74 110.0 75 84.0 76 70.5 77 34.0 78 15.5 79 8.0 80 5.5 81 5.5 82 3.5 83 2.5 84 4.5 85 5.0 86 3.5 87 4.0 88 3.0 89 2.0 90 3.5 91 2.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04273250238617844 2 0.03936773841876282 3 0.023665506570823235 4 0.04306897878292001 5 0.06336972138632761 6 0.0668466441526571 7 0.06617369135917398 8 0.06864118493527877 9 0.06965061412550345 10-11 0.07374441028585912 12-13 0.0722302665005221 14-15 0.06779999394342485 16-17 0.05529428786453012 18-19 0.04895731572589735 20-21 0.05635979645421173 22-23 0.028488334924118966 24-25 0.015477914250111878 26-27 0.01295434127455016 28-29 0.015253596652284169 30-31 0.011215879891385418 32-33 0.006505210337003543 34-35 0.010599006497359221 36-37 0.0063930515380896885 38-39 0.007738957125055939 40-41 0.006505210337003543 42-43 0.004430272557097241 44-45 0.0015141437853370316 46-47 0.002635731774475573 48-49 0.001121587989138542 50-51 0.0054957811467788555 52-53 0.004710669554381876 54-55 0.007738957125055939 56-57 0.02293647437788318 58-59 0.009757815505505314 60-61 0.019964266206666047 62-63 0.013402976470205575 64-65 0.009477418508220679 66-67 0.016711661038164276 68-69 0.026525555943126514 70-71 0.018842678217527505 72-73 0.01951563101101063 74-75 0.01889875761698443 76-77 0.025628285551815684 78-79 0.03712456244048574 80-81 0.02977816111162829 82-83 0.02910520831814516 84-85 0.01990818680720912 86-87 0.023665506570823235 88-89 0.01811364602458745 90-91 0.017721090228388962 92-93 0.029609922913257508 94-95 0.021534489391460004 96-97 0.036451609647002614 98-99 0.054789573269417774 100-101 0.06409875357926767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891593.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.41541447884883 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3183129377361 56.169386003759115 2 10.581899555934537 14.267662890737466 3 2.7552299757437453 5.5723491239793965 4 1.148497640795265 3.0970577792877134 5 0.5634032162582883 1.8991030671384503 6 0.3429082421215008 1.3870380762500654 7 0.2245322093330102 1.0595852369225571 8 0.15983101680158346 0.862005939540368 9 0.1176756694676664 0.7139838628114873 >10 0.7146458181049553 8.951415546280534 >50 0.05154777585323953 2.370646843031528 >100 0.020681990679838214 2.2282697247661196 >500 3.335804681090767E-4 0.16607731786240715 >1k 3.335804681090767E-4 0.6846613327448864 >5k 1.6679023405453836E-4 0.5707572548880018 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.5693180632867239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3749 0.4204833371280393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2340 0.2624515894584188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.10296177740291815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.1215879891385419E-4 0.0 0.0 0.0 0.0 8 1.1215879891385419E-4 0.0 0.0 0.0 0.0 9 1.1215879891385419E-4 0.0 0.0 0.0 0.0 10-11 1.1215879891385419E-4 0.0 0.0 0.0 0.0 12-13 1.1215879891385419E-4 0.0 0.0 0.0 0.0 14-15 1.1215879891385419E-4 0.0 0.0 0.0 0.0 16-17 1.1215879891385419E-4 0.0 0.0 0.0 0.0 18-19 1.1215879891385419E-4 0.0 0.0 0.0 0.0 20-21 1.1215879891385419E-4 0.0 0.0 0.0 0.0 22-23 1.1215879891385419E-4 0.0 0.0 2.2431759782770837E-4 0.0 24-25 4.4863519565541674E-4 0.0 0.0 3.3647639674156256E-4 0.0 26-27 4.4863519565541674E-4 0.0 0.0 4.4863519565541674E-4 0.0 28-29 5.60793994569271E-4 0.0 0.0 0.0014019849864231773 0.0 30-31 5.60793994569271E-4 0.0 0.0 0.0036451609647002612 0.0 32-33 6.16873394026198E-4 0.0 0.0 0.006112654540805054 0.0 34-35 6.729527934831251E-4 0.0 0.0 0.007738957125055939 1.1215879891385419E-4 36-37 6.729527934831251E-4 0.0 0.0 0.01003821250278995 1.1215879891385419E-4 38-39 6.729527934831251E-4 0.0 0.0 0.012954341274550158 1.1215879891385419E-4 40-41 6.729527934831251E-4 0.0 0.0 0.016094787644138075 1.1215879891385419E-4 42-43 7.851115923969794E-4 0.0 0.0 0.024282379964849433 1.1215879891385419E-4 44-45 8.972703913108335E-4 0.0 0.0 0.03605905385080412 1.1215879891385419E-4 46-47 8.972703913108335E-4 0.0 0.0 0.04912555392426814 1.1215879891385419E-4 48-49 8.972703913108335E-4 0.0 0.0 0.07744565065001632 1.1215879891385419E-4 50-51 8.972703913108335E-4 0.0 0.0 0.10711165296273076 1.1215879891385419E-4 52-53 8.972703913108335E-4 0.0 0.0 0.13559998788684974 1.1215879891385419E-4 54-55 8.972703913108335E-4 0.0 0.0 0.17855680787085587 1.1215879891385419E-4 56-57 8.972703913108335E-4 0.0 0.0 0.2291404261810041 1.1215879891385419E-4 58-59 8.972703913108335E-4 0.0 0.0 0.29884711970596445 1.1215879891385419E-4 60-61 8.972703913108335E-4 0.0 0.0 0.3821250278995012 1.1215879891385419E-4 62-63 8.972703913108335E-4 0.0 0.0 0.5005086401530743 1.1215879891385419E-4 64-65 8.972703913108335E-4 0.0 0.0 0.5956753810314797 1.1215879891385419E-4 66-67 0.0010094291902246876 0.0 0.0 0.7343597358884604 1.1215879891385419E-4 68-69 0.0010094291902246876 0.0 0.0 0.9167299429223872 1.1215879891385419E-4 70-71 0.0010094291902246876 0.0 0.0 1.033206855594425 1.1215879891385419E-4 72-73 0.0010094291902246876 0.0 0.0 1.1855185045194387 2.2431759782770837E-4 74-75 0.0010094291902246876 0.0 0.0 1.4000782868416417 2.2431759782770837E-4 76-77 0.0010094291902246876 0.0 0.0 1.5585586697069178 2.2431759782770837E-4 78-79 0.0010094291902246876 0.0 0.0 1.685185953680659 2.2431759782770837E-4 80-81 0.0013459055869662502 0.0 0.0 1.896773527831645 2.2431759782770837E-4 82-83 0.0013459055869662502 0.0 0.0 2.186367546627217 2.2431759782770837E-4 84-85 0.0013459055869662502 0.0 0.0 2.515104986243723 2.2431759782770837E-4 86-87 0.0013459055869662502 0.0 0.0 2.9713109008258254 2.2431759782770837E-4 88-89 0.0013459055869662502 0.0 0.0 3.4422096180656423 2.2431759782770837E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 43.865173 1 GTATCAA 5175 0.0 42.42419 1 ATCAACG 6395 0.0 34.256447 3 TCAACGC 6460 0.0 33.91557 4 CAACGCA 6535 0.0 33.455486 5 TATCAAC 6525 0.0 33.428288 2 AACGCAG 6715 0.0 32.70025 6 ACGCAGA 7515 0.0 28.64837 7 CGCAGAG 7515 0.0 28.521889 8 CGCGAAA 35 0.008396865 27.140156 60-61 GCAGAGT 8005 0.0 26.24168 9 GTATAGG 250 1.4551915E-11 24.710712 1 GAGTACT 4365 0.0 23.463549 12-13 GTACATG 4650 0.0 23.096035 1 GTACTTT 4535 0.0 22.480457 14-15 TACATGG 4765 0.0 22.139715 2 ACATGGG 4630 0.0 21.86153 3 GTGTAGC 525 0.0 21.723705 1 AGTACTT 4515 0.0 21.31562 12-13 AGAGTAC 7370 0.0 20.862286 10-11 >>END_MODULE