FastQCFastQC Report
Thu 26 May 2016
SRR936894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891593
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60700.6808039094070949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.5337637240310321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30850.3460098946492402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.14075929263688702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.046.8765871
GTATCAA52000.039.3820951
TATCAAC70000.029.3844322
ATCAACG69850.029.376233
TCAACGC70500.029.1037544
CAACGCA71350.028.7570385
AACGCAG73900.027.8274566
CGCAGAG83600.024.2010028
ACGCAGA84200.024.141367
TGAACCG1000.001654224823.7478545
GCAGAGT88350.022.5235829
GAGTACT51600.022.13829212-13
AGTACTT53300.021.25396212-13
GTACATG45000.020.8007321
GTACTTT55100.020.56194714-15
TGTACGG1404.8535204E-420.3553035
TACATGG46100.020.0937582
AGAGTAC83050.019.92935210-11
ACATGGG45050.018.8728283
CAGAGTA87600.018.67734510-11
Produced by FastQC (version 0.11.5)