##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936894_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891593 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21556921151243 33.0 31.0 34.0 31.0 34.0 2 32.28697062448898 33.0 31.0 34.0 30.0 34.0 3 32.41380315906473 34.0 31.0 34.0 31.0 34.0 4 36.09973721193415 37.0 35.0 37.0 35.0 37.0 5 36.01717151211371 37.0 35.0 37.0 35.0 37.0 6 36.05200803505635 37.0 35.0 37.0 35.0 37.0 7 36.00814048562517 37.0 35.0 37.0 35.0 37.0 8 35.993615921165826 37.0 35.0 37.0 35.0 37.0 9 37.68909020147085 39.0 37.0 39.0 35.0 39.0 10-11 37.59415787248217 39.0 37.0 39.0 35.0 39.0 12-13 37.52574268752671 39.0 37.0 39.0 35.0 39.0 14-15 38.73784507056471 40.0 38.0 41.0 35.0 41.0 16-17 38.67452021269794 40.0 38.0 41.0 34.5 41.0 18-19 38.73704762150443 40.0 38.0 41.0 34.5 41.0 20-21 38.831550382293265 40.0 38.0 41.0 35.0 41.0 22-23 38.77014175750595 40.0 38.0 41.0 35.0 41.0 24-25 38.6898601716254 40.0 38.0 41.0 34.0 41.0 26-27 38.51374730398287 40.0 38.0 41.0 34.0 41.0 28-29 38.36058268739212 40.0 38.0 41.0 34.0 41.0 30-31 38.2710866953868 40.0 38.0 41.0 34.0 41.0 32-33 38.144882810878954 40.0 37.0 41.0 34.0 41.0 34-35 38.00355879868954 40.0 37.0 41.0 33.5 41.0 36-37 37.82089249242648 40.0 37.0 41.0 33.0 41.0 38-39 37.673863522930304 40.0 37.0 41.0 33.0 41.0 40-41 37.42550973370136 40.0 37.0 41.0 33.0 41.0 42-43 37.17140107650015 40.0 36.5 41.0 32.0 41.0 44-45 36.863221783930555 40.0 36.0 41.0 31.0 41.0 46-47 36.40995779464397 40.0 36.0 41.0 30.5 41.0 48-49 35.9694653277897 39.0 36.0 41.0 29.5 41.0 50-51 35.71279664600328 39.0 35.0 41.0 28.0 41.0 52-53 35.49847407954078 39.0 35.0 40.0 27.5 41.0 54-55 35.23746316985441 39.0 35.0 40.0 27.0 41.0 56-57 35.008520143159494 38.5 35.0 40.0 26.0 41.0 58-59 34.68708031579431 38.0 34.0 40.0 26.0 41.0 60-61 34.377705410428305 38.0 34.0 40.0 25.0 41.0 62-63 34.05838594515659 37.5 34.0 40.0 24.5 41.0 64-65 33.67331338402163 37.0 33.5 40.0 23.0 41.0 66-67 33.25608938159003 36.0 33.0 39.0 22.0 40.5 68-69 32.82908064554118 36.0 32.0 39.0 21.5 40.0 70-71 32.44693150349991 35.0 32.0 38.5 20.0 40.0 72-73 32.07391769563018 35.0 32.0 38.0 18.5 40.0 74-75 31.60161699340394 35.0 31.0 37.0 17.5 39.0 76-77 30.140621337314222 33.5 29.5 35.5 14.0 38.5 78-79 30.82716104769777 34.0 31.0 36.0 14.0 39.0 80-81 30.47210554591613 34.0 31.0 36.0 7.5 37.5 82-83 30.30841482604731 34.0 31.0 35.5 7.5 37.0 84-85 29.983845207398446 34.0 30.0 35.0 6.5 37.0 86-87 29.62601433613768 34.0 30.0 35.0 2.0 36.0 88-89 29.252771163524166 34.0 29.5 35.0 2.0 36.0 90-91 28.828178888797915 34.0 29.0 35.0 2.0 35.5 92-93 28.4311748746345 34.0 29.0 35.0 2.0 35.0 94-95 27.967798648037835 34.0 29.0 35.0 2.0 35.0 96-97 27.3774681945686 33.0 27.0 35.0 2.0 35.0 98-99 26.655222730550825 33.0 25.5 35.0 2.0 35.0 100-101 23.56806581029685 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3424286690093581 1101 2 0.24467299472391346 1101 3 0.24404197691200125 1101 4 0.1984868049167332 1101 5 0.2771405056657912 1101 6 0.29365840499013274 1101 7 0.2841832936255173 1101 8 0.32497922070334084 1101 9 0.41841959396642636 1101 10-11 0.303553432161479 1101 12-13 0.3086029061742934 1101 14-15 0.41492029396829366 1101 16-17 0.4402283484701144 1101 18-19 0.3659216761604114 1101 20-21 0.3067022967144055 1101 22-23 0.449676184450432 1101 24-25 0.3980512590821448 1101 26-27 0.40894256229935877 1101 28-29 0.28859255145109586 1101 30-31 0.42055368237140556 1101 32-33 0.6365519141751506 1101 34-35 0.6541348180580755 1101 36-37 0.5432405391619639 1101 38-39 0.5686325921663169 1101 40-41 0.66768705862237 1101 42-43 0.8035974250699454 1101 44-45 0.8399582166814952 1101 46-47 0.6820033982067528 1101 48-49 0.8007662171388077 1101 50-51 0.7432486089731469 1101 52-53 0.6710060469607555 1101 54-55 0.7265333248515446 1101 56-57 0.5798938278764822 1101 58-59 0.7455613696426653 1101 60-61 0.6933320268201584 1101 62-63 0.6114004543068745 1101 64-65 0.6324847247274619 1101 66-67 0.7105022083888812 1101 68-69 0.7146415963445207 1101 70-71 0.7514058146498499 1101 72-73 0.6958713948815607 1101 74-75 0.6389502398428739 1101 76-77 0.6878523086675479 1101 78-79 0.8655821598270919 1101 80-81 1.0655205779735333 1101 82-83 1.0054243322070846 1101 84-85 0.9017931579999505 1101 86-87 0.7286465302357463 1101 88-89 0.3625666774534224 1101 90-91 0.5826751727644783 1101 92-93 0.7785468554994104 1101 94-95 0.5649278234048616 1101 96-97 0.27749409029606653 1101 98-99 0.6543182692413794 1101 100-101 0.8085831074142824 1102 1 0.2721024875622646 1102 2 0.20402601492886419 1102 3 0.15824683017673635 1102 4 0.16429782606773102 1102 5 0.1438435177960855 1102 6 0.19317827836131585 1102 7 0.1457248299620133 1102 8 0.2069089586609465 1102 9 0.3430040931606797 1102 10-11 0.3102104359743194 1102 12-13 0.1380374058839422 1102 14-15 0.17794166716251425 1102 16-17 0.31824045013510016 1102 18-19 0.2500622071172387 1102 20-21 0.11556075900350748 1102 22-23 0.20311564119265313 1102 24-25 0.22604606610949673 1102 26-27 0.25292631908407515 1102 28-29 0.2708675522242032 1102 30-31 0.34867212887772325 1102 32-33 0.3381578529933691 1102 34-35 0.474509676465388 1102 36-37 0.4731079202155186 1102 38-39 0.5128107131898432 1102 40-41 0.6873992231665298 1102 42-43 0.5371045256067646 1102 44-45 0.5666227682865141 1102 46-47 0.5215712956942227 1102 48-49 0.6741620249644669 1102 50-51 0.5894852500109167 1102 52-53 0.40057546775074115 1102 54-55 0.5509234598069881 1102 56-57 0.4896876895399984 1102 58-59 0.5545601361112276 1102 60-61 0.5395984467041828 1102 62-63 0.3893350164375704 1102 64-65 0.4893816200536705 1102 66-67 0.6572745250571259 1102 68-69 0.565331606572407 1102 70-71 0.5332153491492164 1102 72-73 0.5689711322550401 1102 74-75 0.6109022890283633 1102 76-77 0.5153614914329268 1102 78-79 0.5651333582538527 1102 80-81 0.5777124384985797 1102 82-83 0.517869026590219 1102 84-85 0.5519833417468867 1102 86-87 0.5534610137502902 1102 88-89 0.6304001976681057 1102 90-91 0.593872305290656 1102 92-93 0.3394654985461081 1102 94-95 0.5870744086301976 1102 96-97 0.5728086179062117 1102 98-99 0.9965739939382381 1102 100-101 0.7680386355707576 1103 1 0.26588399236189275 1103 2 0.1322394300640255 1103 3 0.2431694205098438 1103 4 0.196275681460925 1103 5 0.08781275636981434 1103 6 0.21429460661497757 1103 7 0.2225527781801162 1103 8 0.1836062732284489 1103 9 0.2652889793117268 1103 10-11 0.1826154765040897 1103 12-13 0.21731518161085717 1103 14-15 0.38891471518973475 1103 16-17 0.3484600504223252 1103 18-19 0.30644039213116514 1103 20-21 0.36664322015577255 1103 22-23 0.26367422210871183 1103 24-25 0.25103764101306325 1103 26-27 0.30628278223453975 1103 28-29 0.16412098507886697 1103 30-31 0.18527170041839724 1103 32-33 0.2327515609050934 1103 34-35 0.27346752478506886 1103 36-37 0.24507836866880695 1103 38-39 0.2961407519036001 1103 40-41 0.3935869355057591 1103 42-43 0.40288499915077836 1103 44-45 0.5621655052579797 1103 46-47 0.36908270678772226 1103 48-49 0.3887566736290111 1103 50-51 0.4511442735571265 1103 52-53 0.5220853045718101 1103 54-55 0.5113046671381909 1103 56-57 0.41124408767135634 1103 58-59 0.492258185418585 1103 60-61 0.5576379773756983 1103 62-63 0.6574454012769237 1103 64-65 0.40952927061282196 1103 66-67 0.5341808891903881 1103 68-69 0.4401054984191788 1103 70-71 0.6311771903096499 1103 72-73 0.6629642468970864 1103 74-75 0.5236459767555885 1103 76-77 0.5875389650471909 1103 78-79 0.6154609545827725 1103 80-81 0.9451648322381665 1103 82-83 0.7387821998685524 1103 84-85 0.7574663825934245 1103 86-87 0.9894844635027802 1103 88-89 0.7378011238928401 1103 90-91 0.6900032509377674 1103 92-93 0.5307065539516813 1103 94-95 0.7789174267557684 1103 96-97 1.0984637807933915 1103 98-99 1.2129852481999066 1103 100-101 1.2173779055703875 1104 1 0.09053791270683575 1104 2 0.09146443857418518 1104 3 0.0742272328325484 1104 4 0.1189843221054332 1104 5 -0.0036224813471932293 1104 6 0.09793170521931671 1104 7 -0.022353184602138754 1104 8 -0.04489281902164066 1104 9 0.092641328114631 1104 10-11 0.05707472704306582 1104 12-13 -0.08295076074701768 1104 14-15 0.022151481898006864 1104 16-17 0.0851505218849411 1104 18-19 -0.13517887769749848 1104 20-21 -0.053770869824937506 1104 22-23 -0.018312260927714874 1104 24-25 -0.0017529807956222498 1104 26-27 -0.0399234613828412 1104 28-29 0.04305444984682083 1104 30-31 0.06709684509104363 1104 32-33 0.10888766665795657 1104 34-35 0.06219440552943212 1104 36-37 0.04891042075096408 1104 38-39 0.14515570141001177 1104 40-41 0.09113530155590865 1104 42-43 0.1355898811432823 1104 44-45 0.11013583318263898 1104 46-47 0.013310693852282895 1104 48-49 0.14418437985844434 1104 50-51 0.25897148903425915 1104 52-53 0.26716962602874617 1104 54-55 0.0459016403595669 1104 56-57 -0.09538071375835244 1104 58-59 0.12382483411936818 1104 60-61 0.025018906117331596 1104 62-63 -0.004618453256149735 1104 64-65 0.12775305933403303 1104 66-67 -0.024994831336108803 1104 68-69 -0.1862894578953629 1104 70-71 -0.049630281606830806 1104 72-73 -0.02749090458367931 1104 74-75 0.0074901558092612674 1104 76-77 0.019813729599558627 1104 78-79 0.3011341078790366 1104 80-81 0.4358103984471775 1104 82-83 0.3786696530627012 1104 84-85 0.22210207702210383 1104 86-87 0.42806879914330764 1104 88-89 0.42112760182316933 1104 90-91 0.4136363072182583 1104 92-93 0.3025585056139981 1104 94-95 0.3310446110290002 1104 96-97 0.5511022835733748 1104 98-99 0.9909230247799563 1104 100-101 1.3882550898436214 1105 1 0.1387504073335748 1105 2 0.0867431317967089 1105 3 0.18569198422959943 1105 4 0.1658579787897665 1105 5 0.06258775650923099 1105 6 0.10744506673242427 1105 7 0.1033014578967908 1105 8 0.1280599679105876 1105 9 -0.04695513986072086 1105 10-11 0.12934287075993467 1105 12-13 0.08596401930743269 1105 14-15 0.1868119812532143 1105 16-17 0.15925900805774518 1105 18-19 0.24749190250023645 1105 20-21 0.1732875375400198 1105 22-23 0.06257370052808398 1105 24-25 0.10258234661517918 1105 26-27 0.11619265482291752 1105 28-29 0.0849932125635462 1105 30-31 0.08635820058609767 1105 32-33 0.021065270899256916 1105 34-35 -0.07609904625449815 1105 36-37 -0.0524763287833494 1105 38-39 0.24592497351203946 1105 40-41 0.07822424696355768 1105 42-43 -0.07997422188007164 1105 44-45 -0.019544278581221874 1105 46-47 0.0663815134539405 1105 48-49 0.37329089583494834 1105 50-51 0.35719204355496714 1105 52-53 0.29780125305267546 1105 54-55 0.23143726509090357 1105 56-57 0.2062335038946017 1105 58-59 0.29776764469395545 1105 60-61 0.1968885328546861 1105 62-63 0.15972188919978692 1105 64-65 0.1900838015607107 1105 66-67 0.18961103984887728 1105 68-69 0.09983767349882555 1105 70-71 -0.08154835726237053 1105 72-73 0.10258772415969375 1105 74-75 0.07791840484206958 1105 76-77 0.0774634645186012 1105 78-79 0.17572036665536928 1105 80-81 0.28313943914068673 1105 82-83 0.032642733016849945 1105 84-85 -0.25422914879821334 1105 86-87 -0.10049636062170109 1105 88-89 -0.14834420645881963 1105 90-91 0.2510669025757615 1105 92-93 0.5141405732454665 1105 94-95 0.5062483472376265 1105 96-97 0.6828193096516131 1105 98-99 0.6240245615330728 1105 100-101 0.2927529584494373 1106 1 0.03985497495493462 1106 2 0.06713991252551921 1106 3 0.11399622820498934 1106 4 0.06373447138144428 1106 5 0.10995137295941504 1106 6 0.16012662237113062 1106 7 -0.018443086079969362 1106 8 0.020570309803559894 1106 9 0.16511576459768662 1106 10-11 0.13626331862389662 1106 12-13 0.007227810681548874 1106 14-15 0.13590586688084016 1106 16-17 0.050828701463977666 1106 18-19 0.07168548638669137 1106 20-21 0.15253091029150312 1106 22-23 0.27686285849309655 1106 24-25 0.22244559361846683 1106 26-27 0.25223348368358955 1106 28-29 0.2846674704469052 1106 30-31 0.09320895137555851 1106 32-33 0.0668048931096692 1106 34-35 -0.03672334338832428 1106 36-37 0.15960558047629547 1106 38-39 0.1029133891246019 1106 40-41 0.2845438326799581 1106 42-43 0.3070263828599593 1106 44-45 0.194695095759144 1106 46-47 0.22970586477331523 1106 48-49 0.34280497621610806 1106 50-51 0.1202539565667351 1106 52-53 0.1366506119967994 1106 54-55 0.20700451019088462 1106 56-57 0.15157265614760007 1106 58-59 0.22750775321177485 1106 60-61 0.06686890238542986 1106 62-63 0.22253205428228284 1106 64-65 0.1720516486757191 1106 66-67 0.0539511867262874 1106 68-69 0.10840098097612128 1106 70-71 -0.046869608372020366 1106 72-73 0.08119229440124442 1106 74-75 0.35735860632426153 1106 76-77 0.023596010537008993 1106 78-79 0.13286085814026904 1106 80-81 0.21833530888156716 1106 82-83 0.13131230495104163 1106 84-85 -0.01609625481846777 1106 86-87 0.10248195782370928 1106 88-89 -0.09411254596007979 1106 90-91 -0.1258019484915458 1106 92-93 0.28529939977730834 1106 94-95 0.43141360266954365 1106 96-97 0.7788419648690912 1106 98-99 0.815641732796081 1106 100-101 0.5490799331038083 1107 1 0.16424628494461757 1107 2 0.08363686309954943 1107 3 0.07154671618144448 1107 4 0.124794701812867 1107 5 0.14384678413677676 1107 6 0.09295388075619826 1107 7 0.14566930217034724 1107 8 0.10971735838266028 1107 9 0.13311557336828628 1107 10-11 0.13539424929641797 1107 12-13 0.14175940109549146 1107 14-15 0.1723333602048882 1107 16-17 0.2624137883026165 1107 18-19 0.1696734799122126 1107 20-21 0.17471010589868285 1107 22-23 0.158826511247824 1107 24-25 0.24275176554735367 1107 26-27 0.2178156064665231 1107 28-29 0.42025935958849914 1107 30-31 0.3862611774906668 1107 32-33 0.385377707118586 1107 34-35 0.23981656550453323 1107 36-37 0.31543022311635127 1107 38-39 0.3640729905478892 1107 40-41 0.3750619277619478 1107 42-43 0.09564959947505969 1107 44-45 0.3125128951185232 1107 46-47 0.34444009005520826 1107 48-49 0.20111423513388615 1107 50-51 0.39976475078340457 1107 52-53 0.41152750807045635 1107 54-55 0.3887578106054477 1107 56-57 0.2032916718625586 1107 58-59 0.22770069001728643 1107 60-61 0.2722868410690893 1107 62-63 0.6767599314310573 1107 64-65 0.4327775691379543 1107 66-67 0.35961943103551164 1107 68-69 0.5868723068105268 1107 70-71 0.3951895907865719 1107 72-73 0.2915975641371453 1107 74-75 0.30483881749615804 1107 76-77 0.5287967672590632 1107 78-79 0.6170110141971215 1107 80-81 0.5432460115813171 1107 82-83 0.614484117737458 1107 84-85 0.5880110795119897 1107 86-87 0.6958889827723134 1107 88-89 0.4974095034727206 1107 90-91 0.28451146283606477 1107 92-93 0.31988460271950814 1107 94-95 0.6455802864102651 1107 96-97 0.44027031189581933 1107 98-99 0.6045347194578277 1107 100-101 0.40157889158654214 1108 1 0.13705254265519784 1108 2 0.018400755269532 1108 3 7.117422989821875E-4 1108 4 0.11127132726893763 1108 5 -0.030526526097624185 1108 6 0.035894269763204534 1108 7 -0.04299647203759349 1108 8 0.1380030501694094 1108 9 0.0831641336223754 1108 10-11 0.17371091465064126 1108 12-13 0.0722880516454083 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-1.0050965544353723 2316 100-101 -1.6239894070117273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 13.0 6 27.0 7 104.0 8 217.0 9 448.0 10 879.0 11 1457.0 12 2364.0 13 3364.0 14 4251.0 15 6151.0 16 8374.0 17 12166.0 18 10368.0 19 5634.0 20 4512.0 21 4390.0 22 4469.0 23 4867.0 24 5563.0 25 6508.0 26 7944.0 27 9700.0 28 12089.0 29 15418.0 30 19312.0 31 24338.0 32 30985.0 33 41268.0 34 56794.0 35 81431.0 36 126977.0 37 201811.0 38 164228.0 39 13163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54757718987458 20.171516849331514 15.857903989606672 21.423001971187233 2 15.150638221504364 21.53722771833008 40.626556295847635 22.68557776431792 3 18.745295897181073 26.409155712393257 30.990937836858627 23.854610553567042 4 12.453103602204145 18.258106557588498 38.60595585653993 30.682833983667436 5 12.897686422608384 38.146042399695816 34.36089205137495 14.595379126320854 6 29.540608775528742 38.07129486211758 18.047920968423934 14.34017539392974 7 26.449736595060752 34.378915043074585 21.6437320616021 17.527616300262565 8 25.728949709003345 34.75764111571337 20.5058148859735 19.007594289309786 9 26.417942756263802 16.775068388981047 21.196153841840037 35.61083501291512 10-11 23.82342615969394 27.67501539379515 27.932812393098644 20.568746053412262 12-13 25.545092374090387 25.252806216296243 28.603834489317986 20.598266920295384 14-15 22.572575154807183 25.98696939074219 27.839608431201228 23.600847023249397 16-17 20.79928846457969 29.37775419950583 28.212816834587084 21.6101405013274 18-19 20.60145914664205 27.281465898938755 31.884973167472104 20.232101786947094 20-21 21.56674491717522 26.192796840668432 32.2306105670713 20.009847675085048 22-23 21.216014481944114 26.279199141312237 31.86981055257275 20.634975824170894 24-25 20.90297383300637 26.392669787099443 32.23540535902363 20.468951020870556 26-27 20.86891319392646 26.65082514617981 31.9264216147583 20.553840045135434 28-29 20.67517789682433 26.608810940318193 32.107460449924176 20.6085507129333 30-31 20.933166539514428 26.521058654579726 31.98250908764529 20.563265718260556 32-33 20.761385195250483 26.616964946154916 31.834187523238093 20.78746233535651 34-35 21.133374235468434 26.568572579044126 31.778659628807514 20.519393556679926 36-37 21.19493765172013 26.411081346669 31.655101161710665 20.738879839900207 38-39 20.91733723974399 26.78239333457465 31.70692722365921 20.59334220202215 40-41 21.142461026972097 26.49128336739524 31.710999490753935 20.655256114878735 42-43 20.91730348824558 26.794288963890452 31.356746831595323 20.931660716268645 44-45 21.12739641137659 26.96481197441585 30.911176102762834 20.99661551144473 46-47 21.320235919448212 27.126025782449158 30.25537377372976 21.298364524372865 48-49 21.44961753291909 27.45081764956594 29.811682630835147 21.287882186679827 50-51 21.56228373896473 27.53055554620878 29.6539935596973 21.253167155129194 52-53 21.70493814836541 27.675087402096754 29.33154921268012 21.28842523685772 54-55 21.410786008225838 27.74167462070188 29.46445695904242 21.383082412029868 56-57 21.60264696398278 27.99831760763806 29.120529392796556 21.2785060355826 58-59 21.55419842191154 27.487564281588405 29.415310935020216 21.542926361479836 60-61 21.59169037890607 27.6361523699715 29.572069318624077 21.20008793249835 62-63 21.447398084103398 27.606935002854442 29.6863591347173 21.259307778324864 64-65 21.479182089418313 27.471397806043473 29.59614823788052 21.453271866657694 66-67 21.394571022873844 27.91065584250986 29.253207206035086 21.441565928581202 68-69 21.582189960149712 27.648731980165703 29.345089150021252 21.42398890966333 70-71 21.448584346437226 27.671616378802717 29.321912991396047 21.55788628336401 72-73 21.42338347606279 27.684765423221947 29.127184478543473 21.764666622171795 74-75 21.380356193454457 27.833586453970554 28.916490379546357 21.869566973028633 76-77 21.482188933747512 27.772914303083397 29.032270727982713 21.712626035186382 78-79 21.299227767828953 27.86053719182081 28.967203267727154 21.873031772623083 80-81 21.370593275848147 28.050834911035537 28.680439034894885 21.89813277822143 82-83 21.40460030800326 28.076583564111452 28.735885520593936 21.782930607291355 84-85 21.3168645814348 27.836303223183368 28.670769834704952 22.17606236067688 86-87 21.272935072392897 27.66794938946358 28.721793464058155 22.33732207408537 88-89 21.387408687693686 27.592663834999097 28.700020076650183 22.319907400657033 90-91 21.166718633984832 27.732097632757807 28.90758715367895 22.193596579578415 92-93 21.06499564542242 27.709021125729805 28.83195816913706 22.39402505971071 94-95 20.984131684141104 27.755588904292118 28.71101899083433 22.549260420732445 96-97 21.203456070920453 27.64481003330565 28.655027167741242 22.496706728032656 98-99 20.878554536588865 27.523821220415893 28.596848661856157 23.000775581139077 100-101 20.605046069640025 27.720968836398104 28.657895769892942 23.016089324068933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 367.0 1 265.5 2 190.5 3 231.5 4 207.0 5 201.5 6 334.0 7 671.0 8 1153.5 9 1448.0 10 1405.0 11 1290.0 12 1346.5 13 1441.0 14 1562.5 15 1741.0 16 1856.5 17 1956.0 18 2068.0 19 2207.0 20 2423.5 21 2746.5 22 3153.5 23 3707.0 24 4329.0 25 5238.0 26 6593.5 27 8153.5 28 9463.0 29 10934.5 30 12813.5 31 14611.5 32 16509.5 33 18483.0 34 20853.0 35 23207.0 36 25936.0 37 28337.0 38 29384.5 39 29532.5 40 29728.5 41 30760.5 42 31262.0 43 31723.0 44 32873.0 45 33666.0 46 33142.0 47 32359.0 48 33794.0 49 33761.5 50 31210.0 51 29398.5 52 27501.5 53 25975.0 54 24279.0 55 21874.5 56 19539.5 57 17233.0 58 15300.5 59 13432.0 60 11368.0 61 9177.5 62 7005.0 63 4995.0 64 3451.0 65 2567.0 66 1831.0 67 1272.0 68 903.5 69 571.5 70 384.5 71 289.0 72 208.0 73 164.0 74 129.5 75 118.5 76 91.5 77 39.5 78 16.0 79 8.0 80 5.5 81 3.5 82 3.5 83 2.5 84 3.0 85 2.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028264017326291257 2 0.005944416342434272 3 0.009421339108763752 4 0.0 5 0.003364763967415626 6 0.0 7 0.0 8 2.2431759782770837E-4 9 2.2431759782770837E-4 10-11 0.0 12-13 1.1215879891385419E-4 14-15 0.0 16-17 0.0 18-19 0.004991066551666512 20-21 0.0013459055869662502 22-23 0.0 24-25 0.005607939945692709 26-27 0.006056575141348127 28-29 0.0072903219294005225 30-31 0.030619352103482197 32-33 8.411909918539065E-4 34-35 0.003813399163071042 36-37 0.016711661038164276 38-39 0.007514639527228232 40-41 0.008860545114194481 42-43 0.006280892739175834 44-45 0.002860049372303282 46-47 0.0019627789809924484 48-49 0.0014580643858801046 50-51 0.002243175978277084 52-53 0.0026918111739325005 54-55 0.0013459055869662502 56-57 6.16873394026198E-4 58-59 8.972703913108335E-4 60-61 0.0 62-63 0.0 64-65 0.005944416342434272 66-67 7.851115923969792E-4 68-69 6.729527934831251E-4 70-71 0.002916128771760209 72-73 0.011720594486497763 74-75 0.00616873394026198 76-77 0.002972208171217136 78-79 0.009701736106048388 80-81 1.1215879891385419E-4 82-83 0.004934987152209584 84-85 6.16873394026198E-4 86-87 0.0 88-89 0.001121587989138542 90-91 7.851115923969792E-4 92-93 2.803969972846355E-4 94-95 5.60793994569271E-4 96-97 2.803969972846355E-4 98-99 3.925557961984896E-4 100-101 8.972703913108335E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891593.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.13719539944891 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27326136074014 53.590495014044606 2 10.97961989107263 14.303632925129477 3 3.066220166846316 5.991749464367979 4 1.2223847854106236 3.184908664824209 5 0.6349798560004752 2.068040347750845 6 0.390341058981831 1.5255433098796405 7 0.26099814267857024 1.1900480912983094 8 0.18699995530635516 0.9744522102782616 9 0.13291235831824355 0.77917844292993 >10 0.7680764393642978 9.195010390529175 >50 0.05896450069936469 2.5761773246225963 >100 0.02420416333340839 2.771084426685598 >500 3.457737492642427E-4 0.14144275865450118 >1k 5.18660623896364E-4 1.0246717046156897 >5k 1.7288687463212134E-4 0.6835649243891896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6070 0.6808039094070949 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.5337637240310321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 0.3460098946492402 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.14075929263688702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1215879891385419E-4 0.0 0.0 0.0 0.0 3 1.1215879891385419E-4 0.0 0.0 0.0 0.0 4 1.1215879891385419E-4 0.0 0.0 0.0 0.0 5 1.1215879891385419E-4 0.0 0.0 0.0 0.0 6 1.1215879891385419E-4 0.0 0.0 0.0 0.0 7 1.1215879891385419E-4 0.0 0.0 0.0 0.0 8 1.1215879891385419E-4 0.0 0.0 0.0 0.0 9 1.1215879891385419E-4 0.0 0.0 0.0 0.0 10-11 1.1215879891385419E-4 0.0 0.0 0.0 0.0 12-13 1.1215879891385419E-4 0.0 0.0 0.0 0.0 14-15 1.1215879891385419E-4 0.0 0.0 0.0 0.0 16-17 1.6823819837078128E-4 0.0 0.0 0.0 0.0 18-19 2.2431759782770837E-4 0.0 0.0 0.0 0.0 20-21 2.2431759782770837E-4 0.0 0.0 0.0 0.0 22-23 2.2431759782770837E-4 0.0 0.0 2.2431759782770837E-4 0.0 24-25 2.2431759782770837E-4 0.0 0.0 3.3647639674156256E-4 0.0 26-27 2.2431759782770837E-4 0.0 0.0 4.4863519565541674E-4 0.0 28-29 3.3647639674156256E-4 0.0 0.0 0.0014019849864231773 0.0 30-31 3.3647639674156256E-4 0.0 0.0 0.0036451609647002612 0.0 32-33 3.3647639674156256E-4 0.0 0.0 0.00616873394026198 0.0 34-35 3.3647639674156256E-4 0.0 0.0 0.007795036524512866 0.0 36-37 4.4863519565541674E-4 0.0 0.0 0.01003821250278995 0.0 38-39 4.4863519565541674E-4 0.0 0.0 0.012786103076179379 0.0 40-41 4.4863519565541674E-4 0.0 0.0 0.015982628845224223 0.0 42-43 5.047145951123438E-4 0.0 0.0 0.024338459364306363 0.0 44-45 5.60793994569271E-4 0.0 0.0 0.03628337144863183 0.0 46-47 7.290321929400523E-4 0.0 0.0 0.04918163332372506 0.0 48-49 7.851115923969794E-4 0.0 0.0 0.07716525365273169 0.0 50-51 7.851115923969794E-4 0.0 0.0 0.10677517656598919 0.0 52-53 8.411909918539064E-4 0.0 0.0 0.135375670289022 0.0 54-55 8.972703913108335E-4 0.0 0.0 0.17816425207465736 0.0 56-57 0.0010094291902246876 0.0 0.0 0.22908434678154718 0.0 58-59 0.0010094291902246876 0.0 0.0 0.29929575490161986 0.0 60-61 0.0010094291902246876 0.0 0.0 0.383078377690269 0.0 62-63 0.0010094291902246876 0.0 0.0 0.5017984663405837 0.0 64-65 0.0010094291902246876 0.0 0.0 0.5965165720233335 0.0 66-67 0.0010094291902246876 0.0 0.0 0.7357056414754266 0.0 68-69 0.0010094291902246876 0.0 0.0 0.9185805631044659 5.607939945692709E-5 70-71 0.0010094291902246876 0.0 0.0 1.0343284435835634 1.1215879891385419E-4 72-73 0.0010094291902246876 0.0 0.0 1.1859110603156373 1.1215879891385419E-4 74-75 0.0010094291902246876 0.0 0.0 1.3976668726649941 1.1215879891385419E-4 76-77 0.001121587989138542 0.0 0.0 1.5538480001525359 1.1215879891385419E-4 78-79 0.001121587989138542 0.0 0.0 1.6802509665284497 1.1215879891385419E-4 80-81 0.0015702231847939587 0.0 0.0 1.8912777466848665 1.1215879891385419E-4 82-83 0.0015702231847939587 0.0 0.0 2.182049432869033 1.1215879891385419E-4 84-85 0.0016263025842508858 0.0 0.0 2.510618634287169 1.1215879891385419E-4 86-87 0.0016823819837078128 0.0 0.0 2.965254325684477 1.1215879891385419E-4 88-89 0.0016823819837078128 0.0 0.0 3.4358726459270095 1.1215879891385419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2980 0.0 46.876587 1 GTATCAA 5200 0.0 39.382095 1 TATCAAC 7000 0.0 29.384432 2 ATCAACG 6985 0.0 29.37623 3 TCAACGC 7050 0.0 29.103754 4 CAACGCA 7135 0.0 28.757038 5 AACGCAG 7390 0.0 27.827456 6 CGCAGAG 8360 0.0 24.201002 8 ACGCAGA 8420 0.0 24.14136 7 TGAACCG 100 0.0016542248 23.747854 5 GCAGAGT 8835 0.0 22.523582 9 GAGTACT 5160 0.0 22.138292 12-13 AGTACTT 5330 0.0 21.253962 12-13 GTACATG 4500 0.0 20.800732 1 GTACTTT 5510 0.0 20.561947 14-15 TGTACGG 140 4.8535204E-4 20.355303 5 TACATGG 4610 0.0 20.093758 2 AGAGTAC 8305 0.0 19.929352 10-11 ACATGGG 4505 0.0 18.872828 3 CAGAGTA 8760 0.0 18.677345 10-11 >>END_MODULE