FastQCFastQC Report
Thu 26 May 2016
SRR936893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949436
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.5556983303771923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.39612991291672106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.2431970138060912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.044.2505231
GTATCAA53900.038.9588931
ATCAACG66300.031.3191283
TCAACGC66600.031.1083434
CAACGCA67550.030.60055
TATCAAC68000.030.5361522
AACGCAG69250.029.7822486
ACGCAGA78300.026.157917
CGCAGAG78350.025.9592598
AAGACCG1351.33831745E-524.639335
GTACATG49250.024.4054811
GCAGAGT81250.024.3854949
GTCGGGA1201.7065584E-423.7581022
GAGTACT43450.023.6176712-13
TACATGG51100.023.4326482
CATGGGG16850.022.560814
ACATGGG51000.022.3605673
GTACTTT45350.022.20328514-15
AGTACTT45950.020.8335212-13
CAGAGTA79400.020.434610-11
Produced by FastQC (version 0.11.5)