FastQCFastQC Report
Thu 26 May 2016
SRR936893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949436
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62380.6570216423223892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.5127254496353625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31840.3353569908872215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1357648119515165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.051.2775421
GTATCAA56650.041.8465351
ATCAACG75250.031.4964353
TATCAAC76750.030.880872
CAACGCA76900.030.7588715
TCAACGC77050.030.698994
AACGCAG78850.029.9981866
CGCAGAG87850.026.6005578
ACGCAGA88850.026.5684597
GCAGAGT93900.024.279689
TGGATCG1000.001654015923.7485665
GAGTACT56600.023.45485512-13
AGTACTT58150.022.62546212-13
GTACTTT59450.022.45146414-15
GTACATG47300.022.1967831
GTGGTAT16750.021.2718521
AGAGTAC88100.021.05292910-11
TACATGG48850.020.5156482
ACATGGG47600.020.3559113
CAGAGTA92350.020.28978510-11
Produced by FastQC (version 0.11.5)