##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936893_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 949436 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22700634903248 33.0 31.0 34.0 31.0 34.0 2 32.345496694879905 34.0 31.0 34.0 31.0 34.0 3 32.44415000063196 34.0 31.0 34.0 31.0 34.0 4 36.09421066822829 37.0 35.0 37.0 35.0 37.0 5 36.0105199297267 37.0 35.0 37.0 35.0 37.0 6 36.0567747589095 37.0 35.0 37.0 35.0 37.0 7 36.00964993954305 37.0 35.0 37.0 35.0 37.0 8 35.978136493665716 37.0 35.0 37.0 35.0 37.0 9 37.66894451021449 39.0 37.0 39.0 35.0 39.0 10-11 37.586892112791176 39.0 37.0 39.0 35.0 39.0 12-13 37.49523664575601 39.0 37.0 39.0 35.0 39.0 14-15 38.69464977102196 40.0 38.0 41.0 34.5 41.0 16-17 38.61299392481431 40.0 38.0 41.0 34.0 41.0 18-19 38.7940150784255 40.0 38.0 41.0 35.0 41.0 20-21 38.83302350026753 40.0 38.0 41.0 35.0 41.0 22-23 38.75279587039042 40.0 38.0 41.0 34.5 41.0 24-25 38.65173007975261 40.0 38.0 41.0 34.0 41.0 26-27 38.472807540476666 40.0 38.0 41.0 34.0 41.0 28-29 38.3833386347263 40.0 38.0 41.0 34.0 41.0 30-31 38.3320365985701 40.0 38.0 41.0 34.0 41.0 32-33 38.13171872564344 40.0 37.0 41.0 34.0 41.0 34-35 37.96780351703538 40.0 37.0 41.0 33.0 41.0 36-37 37.794465345742104 40.0 37.0 41.0 33.0 41.0 38-39 37.626653086674615 40.0 37.0 41.0 33.0 41.0 40-41 37.428654485399754 40.0 37.0 41.0 33.0 41.0 42-43 37.14925334619711 40.0 36.0 41.0 32.0 41.0 44-45 36.81435820845218 40.0 36.0 41.0 31.0 41.0 46-47 36.36194698747467 39.5 36.0 41.0 30.0 41.0 48-49 35.93048293934504 39.0 35.5 41.0 29.0 41.0 50-51 35.626423476674574 39.0 35.0 40.0 28.0 41.0 52-53 35.38892300270898 39.0 35.0 40.0 27.0 41.0 54-55 35.10794619121247 38.5 35.0 40.0 26.5 41.0 56-57 34.83696057448843 38.0 34.5 40.0 26.0 41.0 58-59 34.499562371765975 38.0 34.0 40.0 25.0 41.0 60-61 34.19493731015045 38.0 34.0 40.0 24.5 41.0 62-63 33.835807784832255 37.0 33.5 40.0 23.0 41.0 64-65 33.46032907957988 37.0 33.0 39.5 22.5 41.0 66-67 33.09264289536104 36.0 33.0 39.0 22.0 40.0 68-69 32.790673094342324 36.0 32.0 39.0 21.5 40.0 70-71 32.334448030198985 35.0 32.0 38.5 19.5 40.0 72-73 31.83538858859365 35.0 31.0 38.0 18.0 40.0 74-75 31.329262846574178 35.0 31.0 37.0 16.0 39.0 76-77 29.90788162656567 33.5 29.5 35.5 13.0 38.0 78-79 30.641721506241602 34.0 30.5 36.0 12.0 38.5 80-81 30.395255709705552 34.0 30.0 36.0 8.0 37.5 82-83 30.07393968629797 34.0 30.0 35.0 7.0 37.0 84-85 29.716372667562638 34.0 30.0 35.0 4.5 36.5 86-87 29.36695575057192 34.0 29.5 35.0 2.0 36.0 88-89 28.973445814146505 34.0 29.0 35.0 2.0 36.0 90-91 28.52790920083081 34.0 29.0 35.0 2.0 35.0 92-93 28.058229833290504 33.5 28.5 35.0 2.0 35.0 94-95 27.47552073020193 33.0 27.0 35.0 2.0 35.0 96-97 26.824751747353165 33.0 25.5 34.0 2.0 35.0 98-99 26.018960730370452 32.5 24.5 34.0 2.0 35.0 100-101 22.566481574324126 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17014092801990444 1101 2 0.3044274175994701 1101 3 0.2527206740964445 1101 4 0.17513823901265368 1101 5 0.1692719550358035 1101 6 0.2238277251927414 1101 7 0.31005504223141855 1101 8 0.28750465418350046 1101 9 0.27853156656161815 1101 10-11 0.3710039188938197 1101 12-13 0.31716056580288665 1101 14-15 0.41348025224074547 1101 16-17 0.42543682748480904 1101 18-19 0.43790430786867063 1101 20-21 0.4207079256306514 1101 22-23 0.37536011332453967 1101 24-25 0.2075187875563529 1101 26-27 0.3008992040531808 1101 28-29 0.22318452544944023 1101 30-31 0.33356211718795237 1101 32-33 0.5479037077637869 1101 34-35 0.4540238827916312 1101 36-37 0.528881494940066 1101 38-39 0.5462020237223442 1101 40-41 0.556182875277635 1101 42-43 0.5856945298987668 1101 44-45 0.5914346299931665 1101 46-47 0.6014394362167295 1101 48-49 0.5356107368517797 1101 50-51 0.4643651147229022 1101 52-53 0.5720457951576137 1101 54-55 0.6371156152674402 1101 56-57 0.6733687158608959 1101 58-59 0.606097173341567 1101 60-61 0.8787028956855139 1101 62-63 0.7111135396831614 1101 64-65 0.5439130530515328 1101 66-67 0.46959643314429655 1101 68-69 0.4546601049971386 1101 70-71 0.5919632045901295 1101 72-73 0.7254205339610671 1101 74-75 0.6457888590874035 1101 76-77 0.5775380903514353 1101 78-79 0.6112021818437832 1101 80-81 0.7778185470428518 1101 82-83 0.6839140263725483 1101 84-85 0.5959746464322677 1101 86-87 0.1449574821449744 1101 88-89 0.2294739432125006 1101 90-91 0.4194283136864847 1101 92-93 0.4707154077239366 1101 94-95 0.5177290080194439 1101 96-97 0.4300981696316306 1101 98-99 0.41964225644099784 1101 100-101 0.9571346559644844 1102 1 0.25206566236353467 1102 2 0.26182045142961385 1102 3 0.27772333222374357 1102 4 0.16926033774488758 1102 5 0.20579274784572732 1102 6 0.169356129628369 1102 7 0.17973112596556007 1102 8 0.25177629603055607 1102 9 0.31041721079785844 1102 10-11 0.2610684644791377 1102 12-13 0.3550535777425736 1102 14-15 0.4217689186113702 1102 16-17 0.4137704631149788 1102 18-19 0.37313450169305185 1102 20-21 0.5036155416344457 1102 22-23 0.37429467335757494 1102 24-25 0.3917035555487516 1102 26-27 0.3914534705038619 1102 28-29 0.4759339672427032 1102 30-31 0.5807929739805644 1102 32-33 0.5338691083279272 1102 34-35 0.5217370176931482 1102 36-37 0.5927009424248482 1102 38-39 0.6861486735437836 1102 40-41 0.6588006615892184 1102 42-43 0.8013856117253084 1102 44-45 0.6655113216566448 1102 46-47 0.6952219200959746 1102 48-49 0.7439619473942187 1102 50-51 0.9488638544578336 1102 52-53 0.9750451009173062 1102 54-55 0.9935335854588843 1102 56-57 0.9795071136042992 1102 58-59 1.122016228674397 1102 60-61 1.052212937152298 1102 62-63 1.1542024086969036 1102 64-65 1.0272769287887726 1102 66-67 0.9128216484526064 1102 68-69 1.141529581564967 1102 70-71 0.9442879714567312 1102 72-73 0.9903323154067145 1102 74-75 0.8500190341485414 1102 76-77 0.8395962939577295 1102 78-79 0.839963744697549 1102 80-81 0.9386849714550962 1102 82-83 0.8043919263894317 1102 84-85 0.9670554722655105 1102 86-87 0.8055527775723696 1102 88-89 0.9627080397932133 1102 90-91 1.06269105549789 1102 92-93 0.9267143319641846 1102 94-95 0.9591060430504328 1102 96-97 1.0822703287189839 1102 98-99 1.165610346404538 1102 100-101 1.5176286298335278 1103 1 0.29197741124900034 1103 2 0.2262811441279311 1103 3 0.19543220687008755 1103 4 0.1492014428895274 1103 5 0.0689530737427475 1103 6 0.13913498522049395 1103 7 -0.007718009605049758 1103 8 0.09215543546063287 1103 9 0.28605455463380736 1103 10-11 0.24503952151719233 1103 12-13 0.29897187860472485 1103 14-15 0.37667089727186465 1103 16-17 0.4324916120812716 1103 18-19 0.39422876689008746 1103 20-21 0.3155324810469011 1103 22-23 0.3778481178556987 1103 24-25 0.2990672358390256 1103 26-27 0.42252643265981504 1103 28-29 0.3426826855654568 1103 30-31 0.4125430142466584 1103 32-33 0.5145903119953417 1103 34-35 0.5813168524350445 1103 36-37 0.39581890535573194 1103 38-39 0.3424032889494413 1103 40-41 0.45420941558924 1103 42-43 0.6541390525767028 1103 44-45 0.7819982344175074 1103 46-47 0.8130380696852555 1103 48-49 0.8711003806938251 1103 50-51 0.8208347048472788 1103 52-53 0.9818666324874457 1103 54-55 0.882718873293058 1103 56-57 0.8056688283015774 1103 58-59 0.8784003033596335 1103 60-61 0.8610812016989939 1103 62-63 0.9336302351485344 1103 64-65 0.6614608642900919 1103 66-67 0.6373405066582265 1103 68-69 0.7247117271246069 1103 70-71 0.5617853351674071 1103 72-73 0.687254466618807 1103 74-75 0.9434150914145185 1103 76-77 0.859055907828715 1103 78-79 0.9719134439934258 1103 80-81 1.036607241186239 1103 82-83 1.0250400736934289 1103 84-85 0.8608532787512395 1103 86-87 0.759564951606972 1103 88-89 0.7371729566323353 1103 90-91 0.8222985446868236 1103 92-93 0.7539138481633074 1103 94-95 0.5360018161745685 1103 96-97 0.42373042274410366 1103 98-99 0.40697501739259323 1103 100-101 0.44418601585010364 1104 1 0.23041348221342872 1104 2 0.265598465396657 1104 3 0.15769412010332218 1104 4 0.08729295359898259 1104 5 0.1731632005683892 1104 6 -0.007192014945545111 1104 7 0.16594535252902887 1104 8 0.27636533757306125 1104 9 0.22744435589994083 1104 10-11 0.3042932739156896 1104 12-13 0.22619226358838773 1104 14-15 0.14258777086193675 1104 16-17 0.3065701318703944 1104 18-19 0.27898116414309015 1104 20-21 0.247697805052141 1104 22-23 0.17859335424775935 1104 24-25 0.23725424966263375 1104 26-27 0.1496833985820487 1104 28-29 0.15058691029863525 1104 30-31 0.39400167762751437 1104 32-33 0.37250539786699477 1104 34-35 0.28441233931990695 1104 36-37 0.4990708800230692 1104 38-39 0.5719543868607317 1104 40-41 0.5364928388702808 1104 42-43 0.5545979668873073 1104 44-45 0.6144230397596928 1104 46-47 0.6514327559668871 1104 48-49 0.5397023898796007 1104 50-51 0.5519431901429215 1104 52-53 0.6238260817403329 1104 54-55 0.5376383448175233 1104 56-57 0.5799537721420052 1104 58-59 0.5113378293421036 1104 60-61 0.5949258824252155 1104 62-63 0.576389601007854 1104 64-65 0.6049788869137132 1104 66-67 0.5786014099304282 1104 68-69 0.5701783253785848 1104 70-71 0.5536185705008165 1104 72-73 0.7450020163085149 1104 74-75 0.7426775327237998 1104 76-77 0.6802135304128889 1104 78-79 0.6610117947233043 1104 80-81 0.9835702421562509 1104 82-83 0.8954691885356141 1104 84-85 0.7426812832604845 1104 86-87 0.6121574554239757 1104 88-89 0.378468565611378 1104 90-91 0.6138820362557098 1104 92-93 0.4966431274206542 1104 94-95 0.6774871829751881 1104 96-97 0.6125107418618292 1104 98-99 0.3698910957475867 1104 100-101 0.5606033756946012 1105 1 0.2275717099221808 1105 2 0.22144384127829397 1105 3 0.17432347872294685 1105 4 0.15692224616501704 1105 5 0.1833640768793643 1105 6 0.16088061464793668 1105 7 0.17243475092759297 1105 8 0.2021416507551521 1105 9 0.1990706687150734 1105 10-11 0.12544950533812482 1105 12-13 0.229096419827151 1105 14-15 0.358817138288714 1105 16-17 0.31030986604138633 1105 18-19 0.2678551705869907 1105 20-21 0.2470614887875371 1105 22-23 0.17573286677225752 1105 24-25 0.07521524868651142 1105 26-27 0.17164034631898772 1105 28-29 0.24026677055859835 1105 30-31 0.3980030574136535 1105 32-33 0.24771328319796027 1105 34-35 0.4470527949428984 1105 36-37 0.37725778232981355 1105 38-39 0.39522983767648867 1105 40-41 0.5601888308851315 1105 42-43 0.6945251611509278 1105 44-45 0.5233266969268655 1105 46-47 0.48141704989069467 1105 48-49 0.5005124270897952 1105 50-51 0.5413441574610687 1105 52-53 0.5870562491494908 1105 54-55 0.7403454411750303 1105 56-57 0.7196461091914585 1105 58-59 0.5957392737139671 1105 60-61 0.5180732626663911 1105 62-63 0.7373192686115075 1105 64-65 0.42544926187793664 1105 66-67 0.48514401816657227 1105 68-69 0.4974672658055823 1105 70-71 0.4128102947197263 1105 72-73 0.5684378305658093 1105 74-75 0.7705521483285942 1105 76-77 0.6187183706732888 1105 78-79 0.8065869380229032 1105 80-81 0.897931393837311 1105 82-83 1.1470607131258994 1105 84-85 0.9989637137390801 1105 86-87 0.9398353781119155 1105 88-89 0.9669154254970316 1105 90-91 0.7276116422093395 1105 92-93 0.7842063370380572 1105 94-95 0.6824012032169442 1105 96-97 0.7292340433671178 1105 98-99 0.892226750742342 1105 100-101 1.099205461520235 1106 1 0.08992177014252434 1106 2 0.08023401225074878 1106 3 0.05217083458298788 1106 4 0.10227594627212255 1106 5 0.08752883619796847 1106 6 0.06197435839813181 1106 7 0.17221786878614864 1106 8 0.1914033181699466 1106 9 0.1302017001940925 1106 10-11 0.12919161113927657 1106 12-13 0.20320033659418613 1106 14-15 0.23070956830200373 1106 16-17 0.0771273428309982 1106 18-19 -0.021138949595943757 1106 20-21 0.1778671970543968 1106 22-23 0.07277606987260299 1106 24-25 0.19790787602699567 1106 26-27 0.21199331311557756 1106 28-29 0.2932980095867137 1106 30-31 0.16632004310742587 1106 32-33 0.24097882604773702 1106 34-35 0.13527138369791913 1106 36-37 0.32874862483692624 1106 38-39 0.3997963622735057 1106 40-41 0.4408320997612165 1106 42-43 0.40043875495022974 1106 44-45 0.31518695016343656 1106 46-47 0.1935686718402323 1106 48-49 0.44140093278072356 1106 50-51 0.44178769921754935 1106 52-53 0.2666884383702737 1106 54-55 0.14521151200838034 1106 56-57 0.34333781656056317 1106 58-59 0.3397459046187876 1106 60-61 0.10334438756472508 1106 62-63 0.3183474144025169 1106 64-65 0.16715863091978633 1106 66-67 0.34824783271456994 1106 68-69 0.12534062085676112 1106 70-71 0.31354316080834366 1106 72-73 0.4663592285667626 1106 74-75 0.319741729097192 1106 76-77 0.3679510612118051 1106 78-79 0.4226557714369754 1106 80-81 0.30148195004223055 1106 82-83 0.6008425954673342 1106 84-85 0.6586320796121434 1106 86-87 0.3865974788465607 1106 88-89 0.4280984109152648 1106 90-91 0.12297045326855027 1106 92-93 0.33401634294898486 1106 94-95 0.3722210754875874 1106 96-97 0.5639542965147974 1106 98-99 0.31001508621634954 1106 100-101 0.38926043758764806 1107 1 0.0507103657037149 1107 2 0.08651052463628872 1107 3 0.04390129236721663 1107 4 0.0305140268766948 1107 5 0.06400377214335151 1107 6 0.09736175418895243 1107 7 0.052469087380117685 1107 8 0.21207865588326769 1107 9 0.08681550595223797 1107 10-11 0.06696352829168006 1107 12-13 0.08247529664959785 1107 14-15 0.23089731032864336 1107 16-17 0.0977730370664247 1107 18-19 0.18600028680783254 1107 20-21 0.07001236709432845 1107 22-23 0.1881395927825622 1107 24-25 0.16539046295402215 1107 26-27 0.16855721901927012 1107 28-29 0.039359626519861024 1107 30-31 -0.002117656677263824 1107 32-33 0.1215912010140201 1107 34-35 -0.038074781967630145 1107 36-37 0.007462236232676389 1107 38-39 0.07291427315928445 1107 40-41 -0.04510157592135755 1107 42-43 0.14927302518214702 1107 44-45 -0.07822202015059787 1107 46-47 -0.03738662875945664 1107 48-49 -0.16162448057287548 1107 50-51 -0.1275987894828532 1107 52-53 -0.20590567226116008 1107 54-55 -0.03413202329254972 1107 56-57 -0.09820079851590435 1107 58-59 -0.05499059944681761 1107 60-61 -0.11520314130555676 1107 62-63 -0.11946106303928161 1107 64-65 -0.1680053518593283 1107 66-67 -0.15901185502103488 1107 68-69 -0.23507367639025034 1107 70-71 -0.19496995198410616 1107 72-73 -0.15995904159796837 1107 74-75 -0.21527512321999254 1107 76-77 -0.2967965196333715 1107 78-79 -0.0704921386484294 1107 80-81 0.050656626211939226 1107 82-83 -0.024584888186755194 1107 84-85 0.09172131537363626 1107 86-87 -0.0730063657148996 1107 88-89 -0.01830469888319186 1107 90-91 0.23437163130036254 1107 92-93 0.140758653183795 1107 94-95 0.3682320514793318 1107 96-97 0.5354669142611606 1107 98-99 0.2496772691422997 1107 100-101 0.7016014126802084 1108 1 0.031984957346345766 1108 2 -0.008961090496896418 1108 3 -0.085066194892633 1108 4 0.06350178736396828 1108 5 -0.044104451365996056 1108 6 0.0428838602476489 1108 7 -0.07173325206834136 1108 8 -0.06532361268575926 1108 9 -0.0571156555952399 1108 10-11 0.041467212101068185 1108 12-13 -0.04850222047782182 1108 14-15 -0.030688916003065003 1108 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-0.6825559733873412 2316 86-87 -0.7671777718824053 2316 88-89 -1.089717721576271 2316 90-91 -1.2008396227541205 2316 92-93 -1.1221041456332657 2316 94-95 -1.4806495305538192 2316 96-97 -1.0526745720158353 2316 98-99 -1.2588207590890512 2316 100-101 -1.0560472336606246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 19.0 6 40.0 7 103.0 8 269.0 9 503.0 10 917.0 11 1665.0 12 2503.0 13 3524.0 14 4488.0 15 6370.0 16 9007.0 17 12823.0 18 11268.0 19 5858.0 20 4842.0 21 4744.0 22 5030.0 23 5457.0 24 6286.0 25 7429.0 26 9079.0 27 11276.0 28 13805.0 29 17471.0 30 21864.0 31 27399.0 32 35259.0 33 46072.0 34 62988.0 35 90117.0 36 140791.0 37 215298.0 38 155292.0 39 9566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36405453727497 20.283222128454014 15.809635789533571 21.543087544737443 2 15.178767042486138 21.557796990073648 40.53499502839712 22.728440939043093 3 18.678918176513587 26.334531942188715 31.12443834958512 23.86211153171258 4 12.490059877716748 18.199034162422915 38.585369193257044 30.725536766603295 5 12.90821689553987 38.081425354474355 34.44357375025541 14.566783999730365 6 29.45885767971722 38.17192522718751 18.09769168221976 14.27152541087551 7 26.440012807603676 34.349234703550316 21.70509649939543 17.50565598945058 8 25.78509767904314 34.81456359354396 20.51491622394769 18.885422503465215 9 26.393248202090504 16.673793704894273 21.172148517646267 35.76080957536896 10-11 23.886154779611598 27.657891299493116 28.020623096013587 20.435330824881706 12-13 25.51088225009374 25.231453199583754 28.585813051116666 20.671851499205847 14-15 22.55760261881791 25.977685699720677 27.75937503949713 23.70533664196428 16-17 20.821308650609414 29.348687009972235 28.215645920314795 21.614358419103553 18-19 20.63023110194069 27.29481230570569 31.788826869188284 20.286129723165335 20-21 21.614650122626788 26.22712366583792 32.03042260819833 20.127803603336964 22-23 21.247425378774114 26.29376197781468 31.84377901468436 20.61503362872685 24-25 20.94140932782209 26.43511437516064 32.119111122347086 20.504365174670188 26-27 20.96132860886638 26.693887826211522 31.72067361573399 20.624109949188103 28-29 20.771596957554163 26.607866691032072 32.01382719274178 20.606709158671986 30-31 20.890456408808824 26.62289922604728 31.882628405792218 20.60401595935168 32-33 20.773260051653892 26.647152505382373 31.6988379980535 20.880749444910236 34-35 21.194530226365966 26.56708825028754 31.683336212235474 20.555045311111016 36-37 21.253080376023128 26.407361196768548 31.530041665064708 20.80951676214362 38-39 21.00625406219089 26.745148809178943 31.673132814285847 20.575464314344313 40-41 21.20325460146931 26.56597414224399 31.58327408694736 20.64749716933934 42-43 21.001520902936058 26.797014227393944 31.26082221445153 20.94064265521847 44-45 21.202692967193155 26.93641277558466 30.782064299226068 21.078829957996113 46-47 21.398320258849502 27.127535199993257 30.182466990790285 21.291677550366956 48-49 21.496836168010933 27.47117265859317 29.7961612698513 21.235829903544598 50-51 21.566734553827565 27.58578193211389 29.476563129529655 21.37092038452889 52-53 21.685400595722093 27.772066784912308 29.28485964298805 21.257672976377552 54-55 21.489195417795433 27.655538375331258 29.409097420298036 21.44616878657527 56-57 21.67544318383499 27.922174701406195 29.04819158454975 21.35419053020907 58-59 21.56807913755068 27.56379975258983 29.26423121949832 21.603889890361167 60-61 21.662834506504492 27.666862224450483 29.418140108550368 21.252163160494657 62-63 21.544947518656816 27.525714014862253 29.605902768634433 21.323435697846502 64-65 21.487546290639916 27.525815326151527 29.49387847100805 21.49275991220051 66-67 21.406012436072512 27.85945668677202 29.235408522401414 21.499122354754057 68-69 21.540709845091673 27.638036405958292 29.35353487478046 21.46771887416957 70-71 21.566350527451657 27.59026925286766 29.26539105046378 21.5779891692169 72-73 21.52767537780324 27.70687018398291 28.98399681495119 21.781457623262654 74-75 21.541209728723157 27.821095892719466 28.830326636023912 21.80736774253346 76-77 21.533994919088805 27.807877518863833 28.974254188802618 21.683873373244747 78-79 21.299070903512366 27.852092389329453 28.95129326734196 21.897543439816225 80-81 21.30672616975549 28.017360099892723 28.740539603978643 21.935374126373144 82-83 21.529308471444697 27.94639767539972 28.69819540104569 21.826098452109896 84-85 21.44151631450633 27.837599126564417 28.549082560834755 22.171801998094494 86-87 21.405729766282246 27.70148926735293 28.57983996090133 22.31294100546349 88-89 21.47186407080839 27.533044635470233 28.65766026233483 22.337431031386544 90-91 21.195725444265623 27.709778056774997 28.89517100750765 22.19932549145173 92-93 21.22781637681839 27.627738014300107 28.761203391285434 22.383242217596074 94-95 21.05692221487283 27.58184859221685 28.757967888304215 22.603261304606104 96-97 21.207624039148193 27.573471326644324 28.663876185199776 22.555028449007708 98-99 20.919120493228224 27.489721339957036 28.586287290588924 23.004870876225816 100-101 20.719734831209784 27.684891930236255 28.56889322363979 23.026480014914164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 249.5 2 179.5 3 208.5 4 203.0 5 203.5 6 324.0 7 660.5 8 1160.5 9 1455.5 10 1386.0 11 1294.5 12 1408.0 13 1539.5 14 1628.5 15 1768.5 16 1904.0 17 2015.5 18 2142.0 19 2276.0 20 2493.0 21 2892.0 22 3362.0 23 3817.5 24 4427.0 25 5493.0 26 6987.5 27 8687.0 28 10053.0 29 11552.0 30 13470.5 31 15555.0 32 17790.5 33 19717.5 34 22122.5 35 24519.0 36 27297.0 37 29932.0 38 31254.5 39 31606.5 40 31823.5 41 32915.0 42 33494.5 43 33934.0 44 34994.0 45 35727.5 46 35187.5 47 34481.0 48 35908.0 49 35817.5 50 33481.0 51 31840.0 52 29659.0 53 27850.5 54 26079.0 55 23530.5 56 20894.5 57 18210.5 58 16324.5 59 14396.5 60 12129.5 61 9772.0 62 7457.0 63 5458.5 64 3759.0 65 2711.0 66 1912.0 67 1369.0 68 980.0 69 626.5 70 428.0 71 298.0 72 199.0 73 169.0 74 140.0 75 112.5 76 86.5 77 39.0 78 13.0 79 10.0 80 4.5 81 2.5 82 2.0 83 1.5 84 3.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03022847248261073 2 0.004634330276079694 3 2.1065137618544064E-4 4 1.0532568809272032E-4 5 2.1065137618544064E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011796477066384673 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.84616972602682E-4 20-21 0.0011059197249735633 22-23 0.0025804793582716477 24-25 0.010953871561642911 26-27 0.005476935780821456 28-29 2.633142202318008E-4 30-31 0.0025278165142252874 32-33 0.004213027523708813 34-35 0.0 36-37 0.008373392203371265 38-39 0.014008316516331802 40-41 0.0011585825690199234 42-43 0.0 44-45 0.0 46-47 4.213027523708813E-4 48-49 0.0035284105511061304 50-51 0.007004158258165901 52-53 0.0 54-55 0.0025278165142252874 56-57 0.0028964564225498085 58-59 5.266284404636016E-5 60-61 0.0015798853213908047 62-63 0.005371610092728736 64-65 0.0 66-67 0.0021591766059007667 68-69 3.6863990832452105E-4 70-71 0.0013165711011590038 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002001188073761686 80-81 0.0018431995416226053 82-83 0.011743814222338314 84-85 0.008584043579556704 86-87 0.004529004587986974 88-89 5.266284404636016E-5 90-91 4.213027523708813E-4 92-93 2.633142202318008E-4 94-95 0.0 96-97 0.0018958623856689656 98-99 0.0018431995416226053 100-101 3.159770642781609E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 949436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5788325363874 #Duplication Level Percentage of deduplicated Percentage of total 1 81.83515663221132 52.84818875740608 2 11.252033153104012 14.532863293763661 3 3.1326651263030083 6.069115697523085 4 1.2755299946492498 3.2948895167837207 5 0.6548591289653761 2.1145019012189774 6 0.3858845588466003 1.4951984582479398 7 0.2639265470682589 1.193084779951964 8 0.18392200494708785 0.9501974685787682 9 0.14215885248967997 0.8262407455646439 >10 0.7871871072071036 9.369640017046398 >50 0.06194031911267027 2.7312105131196383 >100 0.023753664905325194 2.794389698796571 >500 3.2763673013575624E-4 0.132345776538932 >1k 4.914550952036343E-4 0.988202956554887 >5k 1.6381836506787812E-4 0.6599304189047623 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6238 0.6570216423223892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4868 0.5127254496353625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3184 0.3353569908872215 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.1357648119515165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.053256880927203E-4 0.0 0.0 0.0 0.0 14-15 1.053256880927203E-4 0.0 0.0 0.0 0.0 16-17 1.053256880927203E-4 0.0 0.0 0.0 0.0 18-19 1.053256880927203E-4 0.0 0.0 1.053256880927203E-4 0.0 20-21 1.053256880927203E-4 0.0 0.0 1.5798853213908046E-4 0.0 22-23 1.053256880927203E-4 0.0 0.0 4.213027523708812E-4 0.0 24-25 1.053256880927203E-4 0.0 0.0 8.426055047417625E-4 0.0 26-27 1.053256880927203E-4 0.0 0.0 0.0010532568809272032 0.0 28-29 1.5798853213908046E-4 0.0 0.0 0.001632548165437165 0.0 30-31 2.106513761854406E-4 0.0 0.0 0.003054444954688889 0.0 32-33 2.106513761854406E-4 0.0 0.0 0.004897644496311495 0.0 34-35 2.106513761854406E-4 0.0 0.0 0.00689883257007318 1.053256880927203E-4 36-37 2.106513761854406E-4 0.0 0.0 0.009321323396205748 1.053256880927203E-4 38-39 3.159770642781609E-4 0.0 0.0 0.012217779818755556 1.053256880927203E-4 40-41 3.159770642781609E-4 0.0 0.0 0.015324887617490806 1.053256880927203E-4 42-43 4.213027523708812E-4 0.0 0.0 0.02369827982086207 1.053256880927203E-4 44-45 5.266284404636016E-4 0.0 0.0 0.035652745419385824 1.053256880927203E-4 46-47 6.319541285563218E-4 0.0 0.0 0.04971372477976399 1.053256880927203E-4 48-49 6.319541285563218E-4 0.0 0.0 0.07667710093150037 1.053256880927203E-4 50-51 6.319541285563218E-4 0.0 0.0 0.09995407799999156 1.053256880927203E-4 52-53 6.319541285563218E-4 0.0 0.0 0.12644348855531073 1.053256880927203E-4 54-55 6.319541285563218E-4 0.0 0.0 0.1679944725078889 1.053256880927203E-4 56-57 6.319541285563218E-4 0.0 0.0 0.21749754591146742 1.053256880927203E-4 58-59 6.319541285563218E-4 0.0 0.0 0.280166330326636 1.053256880927203E-4 60-61 6.319541285563218E-4 0.0 0.0 0.3530517064867985 1.053256880927203E-4 62-63 6.319541285563218E-4 0.0 0.0 0.46222178219490306 1.053256880927203E-4 64-65 6.319541285563218E-4 0.0 0.0 0.5560143074414705 1.053256880927203E-4 66-67 6.319541285563218E-4 0.0 0.0 0.6835110528777084 1.053256880927203E-4 68-69 6.319541285563218E-4 0.0 0.0 0.8515581882296437 1.053256880927203E-4 70-71 6.319541285563218E-4 0.0 0.0 0.9519335689820061 1.053256880927203E-4 72-73 6.319541285563218E-4 0.0 0.0 1.094017922219086 2.106513761854406E-4 74-75 6.319541285563218E-4 0.0 0.0 1.284710080510956 2.106513761854406E-4 76-77 7.372798166490422E-4 0.0 0.0 1.4327453351252744 2.106513761854406E-4 78-79 7.372798166490422E-4 0.0 0.0 1.5527639567069291 2.106513761854406E-4 80-81 7.899426606954023E-4 0.0 0.0 1.7481957709629716 2.106513761854406E-4 82-83 9.479311928344828E-4 0.0 0.0 2.0097721173412424 2.106513761854406E-4 84-85 0.001000594036880843 0.0 0.0 2.3128994476720917 2.106513761854406E-4 86-87 0.0010532568809272032 0.0 0.0 2.746946608302192 2.106513761854406E-4 88-89 0.0010532568809272032 0.0 0.0 3.1908417207689617 2.106513761854406E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3150 0.0 51.277542 1 GTATCAA 5665 0.0 41.846535 1 ATCAACG 7525 0.0 31.496435 3 TATCAAC 7675 0.0 30.88087 2 CAACGCA 7690 0.0 30.758871 5 TCAACGC 7705 0.0 30.69899 4 AACGCAG 7885 0.0 29.998186 6 CGCAGAG 8785 0.0 26.600557 8 ACGCAGA 8885 0.0 26.568459 7 GCAGAGT 9390 0.0 24.27968 9 TGGATCG 100 0.0016540159 23.748566 5 GAGTACT 5660 0.0 23.454855 12-13 AGTACTT 5815 0.0 22.625462 12-13 GTACTTT 5945 0.0 22.451464 14-15 GTACATG 4730 0.0 22.196783 1 GTGGTAT 1675 0.0 21.271852 1 AGAGTAC 8810 0.0 21.052929 10-11 TACATGG 4885 0.0 20.515648 2 ACATGGG 4760 0.0 20.355911 3 CAGAGTA 9235 0.0 20.289785 10-11 >>END_MODULE