FastQCFastQC Report
Thu 26 May 2016
SRR936892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974800
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54310.5571399261386951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.4025441116126385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.25266721378744356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.040.583731
GTATCAA54500.040.4806821
TATCAAC68500.031.9345322
ATCAACG68500.031.9345323
TCAACGC69550.031.384044
CAACGCA69750.031.1576925
AACGCAG71350.030.5225056
ACGCAGA80000.026.8545977
CGCAGAG80400.026.5436388
GTACATG50200.025.3838461
GCAGAGT83300.024.5329069
TACATGG50800.024.5262342
ACATGGG50450.023.8480383
GAGTACT42850.022.3525912-13
CATGGGA33150.022.091774
GTCCACG1353.769917E-421.1321581
AGTACTT45850.020.7863712-13
GTGGTAT18450.020.359031
AGAGTAC75900.020.25516110-11
GTACTTT46950.020.19915814-15
Produced by FastQC (version 0.11.5)