##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936892_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 974800 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.841435166187935 33.0 31.0 34.0 30.0 34.0 2 31.925893516618792 33.0 31.0 34.0 30.0 34.0 3 31.935526261797293 33.0 31.0 34.0 30.0 34.0 4 35.550503693065245 37.0 35.0 37.0 33.0 37.0 5 35.55815449322938 37.0 35.0 37.0 33.0 37.0 6 35.6308483791547 37.0 35.0 37.0 33.0 37.0 7 35.58522158391465 37.0 35.0 37.0 33.0 37.0 8 35.5504195732458 37.0 35.0 37.0 33.0 37.0 9 37.189877923676654 39.0 37.0 39.0 34.0 39.0 10-11 37.07195629872794 39.0 37.0 39.0 33.0 39.0 12-13 36.98914392695937 39.0 37.0 39.0 33.0 39.0 14-15 38.13634386540829 40.0 38.0 41.0 33.0 41.0 16-17 38.08087864177267 40.0 38.0 41.0 33.0 41.0 18-19 38.07041752154288 40.0 38.0 41.0 33.0 41.0 20-21 38.02907775954042 40.0 38.0 41.0 33.0 41.0 22-23 37.92392593352483 40.0 38.0 41.0 33.0 41.0 24-25 37.888480713992614 40.0 38.0 41.0 33.0 41.0 26-27 37.90160853508412 40.0 38.0 41.0 33.0 41.0 28-29 37.82641464915881 40.0 38.0 41.0 33.0 41.0 30-31 37.65903108329914 40.0 37.0 41.0 33.0 41.0 32-33 37.4588838736151 40.0 37.0 41.0 32.0 41.0 34-35 37.29754052113254 40.0 36.0 41.0 31.5 41.0 36-37 37.12248871563398 40.0 36.0 41.0 31.0 41.0 38-39 36.967627718506364 39.0 36.0 41.0 31.0 41.0 40-41 36.77811653672548 39.0 36.0 41.0 30.5 41.0 42-43 36.55668188346328 39.0 35.5 41.0 30.0 41.0 44-45 36.28287853918752 39.0 35.0 41.0 30.0 41.0 46-47 35.89310730406237 39.0 35.0 40.5 28.5 41.0 48-49 35.61527851867049 39.0 35.0 40.5 27.0 41.0 50-51 34.915780672958554 38.0 34.0 40.0 26.5 40.5 52-53 35.09300625769389 38.5 35.0 40.0 27.0 40.5 54-55 35.485008206811656 39.0 35.0 40.5 27.0 41.0 56-57 35.38902749281904 39.0 35.0 41.0 27.0 41.0 58-59 35.193045240049244 39.0 35.0 41.0 26.0 41.0 60-61 34.92798881821912 38.5 35.0 40.0 26.0 41.0 62-63 34.6332960607304 38.0 34.0 40.0 26.0 41.0 64-65 34.32717480508822 37.5 34.0 40.0 25.0 41.0 66-67 33.9788910545753 37.0 34.0 40.0 24.5 41.0 68-69 33.59844121871153 36.5 34.0 39.0 23.0 41.0 70-71 33.19746512105047 36.0 33.0 39.0 22.0 41.0 72-73 32.81710196963479 35.5 33.0 39.0 22.0 40.0 74-75 32.42039495281084 35.0 32.5 38.0 20.5 40.0 76-77 31.973573553549443 35.0 32.0 37.0 19.5 39.0 78-79 31.51625 35.0 31.5 37.0 18.0 39.0 80-81 31.08576066885515 35.0 31.0 36.0 15.0 38.5 82-83 30.691379257283543 35.0 31.0 36.0 11.0 37.0 84-85 30.322452810832992 34.5 31.0 35.0 7.5 37.0 86-87 29.97441680344686 34.0 30.5 35.0 4.5 36.5 88-89 29.623136540828888 34.0 30.0 35.0 2.0 36.0 90-91 29.26724097250718 34.0 30.0 35.0 2.0 36.0 92-93 28.904805088223224 34.0 29.0 35.0 2.0 35.5 94-95 28.560637053754615 34.0 29.0 35.0 2.0 35.0 96-97 28.1067618998769 34.0 29.0 35.0 2.0 35.0 98-99 27.588047291752154 34.0 29.0 35.0 2.0 35.0 100-101 25.480634489125976 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32754258357477894 1101 2 0.29890563503570533 1101 3 0.41862270435678894 1101 4 0.35143623543642377 1101 5 0.261122395129739 1101 6 0.2096856549237529 1101 7 0.19475330809535052 1101 8 0.21568658298085097 1101 9 0.14755041507999778 1101 10-11 0.18818355118769858 1101 12-13 0.3324323924444528 1101 14-15 0.3839317365489663 1101 16-17 0.45996926672464156 1101 18-19 0.3968919046933266 1101 20-21 0.44956252177163236 1101 22-23 0.5059099140461072 1101 24-25 0.5903159103252094 1101 26-27 0.4793700239484977 1101 28-29 0.4305760621872068 1101 30-31 0.43136570706189303 1101 32-33 0.4398376505230246 1101 34-35 0.5288790615754309 1101 36-37 0.6283840380489849 1101 38-39 0.5710054971338536 1101 40-41 0.7830013017228907 1101 42-43 0.687430687983472 1101 44-45 0.7201602254465413 1101 46-47 0.8085731052768779 1101 48-49 0.9820485673595769 1101 50-51 0.8671731010724173 1101 52-53 0.9772087338428364 1101 54-55 1.1031073864364984 1101 56-57 1.0238769081309513 1101 58-59 1.0109688488661561 1101 60-61 1.2229157141387645 1101 62-63 1.2067671015413097 1101 64-65 0.9875705226868945 1101 66-67 0.9992098028224419 1101 68-69 0.9865284078719796 1101 70-71 1.0472295010708947 1101 72-73 0.9635683418842547 1101 74-75 0.8894922140188939 1101 76-77 1.0279738892144756 1101 78-79 1.0828889874413221 1101 80-81 0.9379871698398574 1101 82-83 0.7637915818783618 1101 84-85 0.7189021360509216 1101 86-87 0.7578830239619769 1101 88-89 0.8833598740346176 1101 90-91 0.9405838771786321 1101 92-93 1.0670819172208148 1101 94-95 1.2623111188101852 1101 96-97 1.3444058721038097 1101 98-99 1.1680911323106429 1101 100-101 1.4007762698269985 1102 1 0.3237579954017029 1102 2 0.186786232117381 1102 3 0.2524569226241091 1102 4 0.3127132460477995 1102 5 0.4109987950640033 1102 6 0.2860884961040995 1102 7 0.2791971413409229 1102 8 0.09928450207227968 1102 9 0.2467697948547567 1102 10-11 0.4057579599925347 1102 12-13 0.3282721405708813 1102 14-15 0.1698341735554223 1102 16-17 0.30483804717045615 1102 18-19 0.27323202345225184 1102 20-21 0.2307174528608229 1102 22-23 0.21891318880496868 1102 24-25 0.09205698859182831 1102 26-27 0.20696604459658374 1102 28-29 0.24825031256482788 1102 30-31 0.18555838229055865 1102 32-33 0.22390034163913697 1102 34-35 0.22775553641881885 1102 36-37 0.10499617757674429 1102 38-39 0.30675068086806334 1102 40-41 -0.05146545148059545 1102 42-43 0.1578228540536557 1102 44-45 0.14406512662798576 1102 46-47 0.2909490551973235 1102 48-49 0.39966138106951377 1102 50-51 0.19060081991050026 1102 52-53 0.3960508399298135 1102 54-55 0.2174846847213061 1102 56-57 0.3926523227306191 1102 58-59 0.22644692005666656 1102 60-61 0.3120321441708356 1102 62-63 0.14774653283566153 1102 64-65 0.2540167105388704 1102 66-67 -0.048923506361077784 1102 68-69 0.08290487433001914 1102 70-71 0.17245129359049116 1102 72-73 0.22344852081645428 1102 74-75 0.4868813513005037 1102 76-77 0.2919192860483939 1102 78-79 0.3039567332466895 1102 80-81 0.33705354491993944 1102 82-83 0.32074963388319233 1102 84-85 0.2949016418742687 1102 86-87 0.3602415994362751 1102 88-89 0.4376256695192744 1102 90-91 0.2188919817875572 1102 92-93 0.45701999979303665 1102 94-95 0.38123998532323 1102 96-97 0.2245431570615004 1102 98-99 0.35715590858390556 1102 100-101 0.209042436503168 1103 1 0.2580353762709713 1103 2 0.16995649866480633 1103 3 0.2068065338236842 1103 4 0.22974145222450204 1103 5 0.21671328024836356 1103 6 0.17613837470439364 1103 7 0.30990057255865366 1103 8 0.21960152651068654 1103 9 0.21497869764744593 1103 10-11 0.2757527848522727 1103 12-13 0.27340734249555254 1103 14-15 0.48130364743094134 1103 16-17 0.5070771541650956 1103 18-19 0.47038353538000877 1103 20-21 0.4156519768088671 1103 22-23 0.474030150299491 1103 24-25 0.40783446199305473 1103 26-27 0.485728798214474 1103 28-29 0.57619379826005 1103 30-31 0.5766327613568407 1103 32-33 0.6000762454325042 1103 34-35 0.4828259548959579 1103 36-37 0.5016834229061047 1103 38-39 0.6159173835501548 1103 40-41 0.581973656400578 1103 42-43 0.48807548290302094 1103 44-45 0.5123530971418759 1103 46-47 0.4536639980546937 1103 48-49 0.6763399494506217 1103 50-51 0.6602500628661616 1103 52-53 0.6341352733960193 1103 54-55 0.6873104032845418 1103 56-57 0.6924322961567739 1103 58-59 0.7562064699635229 1103 60-61 0.6328512237050958 1103 62-63 0.8613958821753513 1103 64-65 0.9340409461529191 1103 66-67 1.014484428780115 1103 68-69 1.0683740334860872 1103 70-71 0.8072772725729962 1103 72-73 0.8934795827397224 1103 74-75 0.9387791428343206 1103 76-77 0.845031794927575 1103 78-79 1.0247113728652124 1103 80-81 1.1121485425429185 1103 82-83 1.1189693080599064 1103 84-85 0.7439805333969005 1103 86-87 0.9349204249121534 1103 88-89 0.9424029778035994 1103 90-91 1.0697332468267682 1103 92-93 0.9418660920080519 1103 94-95 1.0206634724812353 1103 96-97 0.8307642962350918 1103 98-99 0.9135990136237382 1103 100-101 1.2665291578911244 1104 1 0.11823703028705523 1104 2 0.08986133609541724 1104 3 0.0715882910943968 1104 4 0.1532958065594201 1104 5 0.1558205647164712 1104 6 0.047474696703460495 1104 7 0.10117410020920659 1104 8 0.1459071796204654 1104 9 0.20531678371454376 1104 10-11 0.20622584640044295 1104 12-13 0.16244503528876209 1104 14-15 0.044146548270894925 1104 16-17 0.32509087829074446 1104 18-19 0.3193331866124325 1104 20-21 0.20961206679252342 1104 22-23 0.17644077354957943 1104 24-25 0.14161798333179831 1104 26-27 0.20204561836045087 1104 28-29 0.27477283336338587 1104 30-31 0.48031707409858626 1104 32-33 0.11006993362943263 1104 34-35 0.3567760107311386 1104 36-37 0.25582250915122273 1104 38-39 0.3269379535545269 1104 40-41 0.12363964872930922 1104 42-43 0.1802456102719603 1104 44-45 0.16263470066353847 1104 46-47 0.23973433419866552 1104 48-49 0.19077212993582293 1104 50-51 0.22942499691590257 1104 52-53 0.2584980240474266 1104 54-55 0.583416506312588 1104 56-57 0.5121898581823814 1104 58-59 0.3589310649561597 1104 60-61 0.4672999119829484 1104 62-63 0.21232808693672212 1104 64-65 0.3244300121679018 1104 66-67 0.31007506789063655 1104 68-69 0.32649845692714763 1104 70-71 0.34835254487065015 1104 72-73 0.3564907983282595 1104 74-75 0.2940527352213209 1104 76-77 0.4715470322675621 1104 78-79 0.55387109385115 1104 80-81 0.3818122367279706 1104 82-83 0.3493582122337848 1104 84-85 0.36541800608621244 1104 86-87 0.48590441882693014 1104 88-89 0.4523683438072581 1104 90-91 0.5274684424117417 1104 92-93 0.5351027522550211 1104 94-95 0.6095992053786752 1104 96-97 0.5411496399046776 1104 98-99 0.4362244000193769 1104 100-101 0.6451604676711824 1105 1 0.02409105851282689 1105 2 0.12619254063519847 1105 3 0.08868707600526804 1105 4 0.13886670568312098 1105 5 0.1272066218889023 1105 6 0.1769187237805525 1105 7 -0.003809045924967336 1105 8 0.21115269944966997 1105 9 0.3055883732328013 1105 10-11 0.309098463390562 1105 12-13 0.15101206719953808 1105 14-15 0.35864608813989207 1105 16-17 0.4833653804316427 1105 18-19 0.2782798629086187 1105 20-21 0.309594176476665 1105 22-23 0.33068962454239426 1105 24-25 0.20884266061295165 1105 26-27 0.4167663775964101 1105 28-29 0.38259631470002375 1105 30-31 0.3636401969687526 1105 32-33 0.0848944302910013 1105 34-35 0.015653568353130254 1105 36-37 0.06357687181367311 1105 38-39 0.37679582126653344 1105 40-41 0.31449135979609366 1105 42-43 0.3233450315763591 1105 44-45 0.4409849098679999 1105 46-47 0.5374945458721285 1105 48-49 0.46014021229201063 1105 50-51 0.2579940489140071 1105 52-53 0.41587008848425455 1105 54-55 0.39364646618810895 1105 56-57 0.36670561156272896 1105 58-59 0.43877006531660356 1105 60-61 0.19896629918218878 1105 62-63 0.23359049628034967 1105 64-65 0.30396573536577165 1105 66-67 0.3421000535981875 1105 68-69 0.1539324971150009 1105 70-71 -0.023225024502181668 1105 72-73 9.83815823794032E-4 1105 74-75 0.18096481389117258 1105 76-77 0.16761169090581163 1105 78-79 0.285181985290599 1105 80-81 0.26660718466121125 1105 82-83 0.32686823675210164 1105 84-85 0.32586423413426857 1105 86-87 0.31479807113160874 1105 88-89 0.063774509430651 1105 90-91 0.224055947181391 1105 92-93 0.35168839537556806 1105 94-95 0.3104417666381529 1105 96-97 0.1447959635437961 1105 98-99 0.35432933365356334 1105 100-101 0.5191733645424215 1106 1 0.2984246529570207 1106 2 0.22373654262451126 1106 3 0.2701579929796516 1106 4 0.3923745165189132 1106 5 0.35128302354926433 1106 6 0.3214253388247812 1106 7 0.4029315182272555 1106 8 0.30840814989136334 1106 9 0.44678513228971184 1106 10-11 0.46452463280936485 1106 12-13 0.41605048274602296 1106 14-15 0.38077629447970907 1106 16-17 0.409536920931977 1106 18-19 0.42968031389771255 1106 20-21 0.4514910973770512 1106 22-23 0.33637706041393045 1106 24-25 0.3595289465216993 1106 26-27 0.257708277742303 1106 28-29 0.22280155971847648 1106 30-31 0.3236214920390168 1106 32-33 0.3729923578968197 1106 34-35 0.44960242174263954 1106 36-37 0.24082093295604778 1106 38-39 3.637781263847728E-4 1106 40-41 0.3318086960579336 1106 42-43 0.3073138714039416 1106 44-45 0.18473628367041073 1106 46-47 0.13287780597843124 1106 48-49 -0.01016740911817493 1106 50-51 0.10502438671451841 1106 52-53 0.06556935836397315 1106 54-55 0.2284494321585484 1106 56-57 0.3453226688631261 1106 58-59 0.42581876857443746 1106 60-61 0.4894133758579784 1106 62-63 0.44327157043894516 1106 64-65 0.29622958350618234 1106 66-67 0.22719967601999969 1106 68-69 0.2073947337798856 1106 70-71 0.44187042240900354 1106 72-73 0.4148456624348853 1106 74-75 0.5470993858461739 1106 76-77 0.5125479082835689 1106 78-79 0.48083266478705866 1106 80-81 0.6330379462930225 1106 82-83 0.46462995937089246 1106 84-85 0.5082525752938558 1106 86-87 0.5434985113424524 1106 88-89 0.6591259439986175 1106 90-91 0.5729737515756952 1106 92-93 0.6448902906500287 1106 94-95 0.8887754493451752 1106 96-97 0.948927173024579 1106 98-99 1.0428056881272596 1106 100-101 1.1468400263998966 1107 1 0.2207036463437042 1107 2 0.3470639296513518 1107 3 0.33786246345173154 1107 4 0.15429408024819224 1107 5 0.2929654130730981 1107 6 0.23771383792140455 1107 7 0.3815876681975965 1107 8 0.40599081059877307 1107 9 0.38133652517677064 1107 10-11 0.39382662937413926 1107 12-13 0.5603708839102879 1107 14-15 0.4742980583042993 1107 16-17 0.6612931002719691 1107 18-19 0.7041156909548718 1107 20-21 0.7268738456220092 1107 22-23 0.6589420936176396 1107 24-25 0.5116957295622697 1107 26-27 0.5672706699631149 1107 28-29 0.5028925942043969 1107 30-31 0.6762938444565307 1107 32-33 0.5777993810142732 1107 34-35 0.6753093217818389 1107 36-37 0.4901732579009348 1107 38-39 0.6701912057221904 1107 40-41 0.669556238206809 1107 42-43 0.9748865784285456 1107 44-45 0.8376161663746586 1107 46-47 0.7067441889289583 1107 48-49 0.5574559234114034 1107 50-51 0.4115943887601077 1107 52-53 0.22025427459190183 1107 54-55 0.5205305975279586 1107 56-57 0.5278053299133703 1107 58-59 0.7684117315149663 1107 60-61 0.9005684716064621 1107 62-63 0.6291940231361011 1107 64-65 0.7170378088365297 1107 66-67 0.8165954040501902 1107 68-69 0.8416374596490712 1107 70-71 0.8652196366851612 1107 72-73 0.6309982840475143 1107 74-75 0.8473674838928034 1107 76-77 0.8661744886164193 1107 78-79 0.7430350685271776 1107 80-81 0.7428512947416266 1107 82-83 0.8261343482479084 1107 84-85 0.8375183454814881 1107 86-87 0.8737310801125773 1107 88-89 0.5075494109200491 1107 90-91 0.4929448847527702 1107 92-93 0.6044498830404308 1107 94-95 0.5842178968312126 1107 96-97 0.6550604015261676 1107 98-99 1.0970206899614325 1107 100-101 1.292188936694476 1108 1 0.01731163988798201 1108 2 0.0681724986171055 1108 3 -0.007710229933447721 1108 4 -0.034054437181801234 1108 5 0.1282034449940852 1108 6 0.08430440834519715 1108 7 0.05250848011501574 1108 8 0.042668313223707344 1108 9 0.040667523200625055 1108 10-11 -0.19440829389534997 1108 12-13 -0.09959743352062134 1108 14-15 0.11084488752562294 1108 16-17 0.1380091630135425 1108 18-19 0.11821569508140328 1108 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-2.199889931116118 2316 68-69 -2.3705825375862553 2316 70-71 -1.9094826850090492 2316 72-73 -2.3058155403498617 2316 74-75 -2.454762838902205 2316 76-77 -2.5157719197770376 2316 78-79 -2.612719834017973 2316 80-81 -2.334740367602592 2316 82-83 -3.089521457129006 2316 84-85 -3.124750160413736 2316 86-87 -3.17336987750544 2316 88-89 -2.855094989531935 2316 90-91 -2.921989314676793 2316 92-93 -3.116426849162597 2316 94-95 -3.2965402079195947 2316 96-97 -3.6601254088347766 2316 98-99 -4.461347592857372 2316 100-101 -4.643612229796123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1526.0 3 518.0 4 536.0 5 606.0 6 654.0 7 780.0 8 945.0 9 1241.0 10 1591.0 11 2191.0 12 3018.0 13 3923.0 14 4501.0 15 5977.0 16 7658.0 17 10509.0 18 9539.0 19 5146.0 20 4823.0 21 5107.0 22 5409.0 23 6008.0 24 6747.0 25 7799.0 26 9204.0 27 11132.0 28 13360.0 29 16121.0 30 19925.0 31 24911.0 32 32397.0 33 42136.0 34 58170.0 35 85132.0 36 129454.0 37 199982.0 38 198476.0 39 37639.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40314694931466 19.944088899470856 15.003427736339342 21.649336414875144 2 14.899009852221804 21.117371865190492 41.308320873965236 22.675297408622463 3 18.612795649274023 26.589400236006362 30.518495715971472 24.27930839874814 4 12.13814155331754 17.66491220147063 39.14558092368076 31.051365321531065 5 13.157521796728181 38.06206804481551 34.40073117882046 14.379678979635848 6 29.422344846855026 37.88173251349225 18.50774815566572 14.188174483987007 7 26.71436783969121 34.17053340450869 21.764464337042664 17.350634418757444 8 25.500684420173403 34.90532615470874 20.757288073631905 18.836701351485956 9 26.547400498585148 17.044301795974356 21.26666913084369 35.1416285745968 10-11 23.825788125660655 27.723870195355875 28.133443470109317 20.316898208874157 12-13 25.406308634908736 25.3106190306294 28.469608026838266 20.813464307623597 14-15 22.630965128324114 26.178960779502635 27.37175545168301 23.818318640490237 16-17 21.098154789125722 29.539028332309385 27.847281353726068 21.515535524838825 18-19 20.903370726534114 27.817245907092254 30.81007080650841 20.469312559865223 20-21 22.160178988370784 26.833832661887413 30.766731527195994 20.2392568225458 22-23 21.648017602609194 26.93593294752366 30.546356171637758 20.869693278229395 24-25 21.302615048649027 27.094380700236126 30.799319370196848 20.803684880918 26-27 21.220180648576502 27.310773719422777 30.565351358865282 20.903694273135436 28-29 21.136086515589948 27.277166801150493 30.737872474452775 20.848874208806784 30-31 21.23174890431871 27.22778270218808 30.632037803056416 20.908430590436794 32-33 20.956960526673516 27.354634118566175 30.481988957871557 21.206416396888752 34-35 21.44656940042864 27.274592623674863 30.400025443386923 20.87881253250957 36-37 21.48262710668934 27.11505069391545 30.30488164580175 21.097440553593458 38-39 21.257065298516075 27.370295764078143 30.3692293329599 21.003409604445874 40-41 21.485474683804036 27.236660675153274 30.419754758328615 20.858109882714075 42-43 21.255121784073587 27.323858844964594 30.09490560734049 21.32611376362133 44-45 21.385726332462703 27.601320921567464 29.80521638861931 21.207736357350523 46-47 21.540497495595172 27.728082931320575 29.283147779119957 21.448271793964295 48-49 21.637080149549163 27.93613846582338 28.968353907713045 21.458427476914412 50-51 21.707903575598035 28.087228424229615 28.801188788375566 21.403679211796785 52-53 21.823894370477902 28.097288729414117 28.661343811475515 21.417473088632462 54-55 21.617419149306187 28.1176912978639 28.732526446632463 21.532363106197447 56-57 21.690271765142224 28.45457012455838 28.412915577210445 21.442242533088944 58-59 21.708667524329513 28.032392161207788 28.61490527749907 21.644035036963626 60-61 21.728398734667433 28.125847962095424 28.790060358230484 21.35569294500666 62-63 21.663679389015368 28.06756821919607 28.924097840439362 21.344654551349198 64-65 21.613873356583486 28.02765397243733 28.843854464763396 21.514618206215783 66-67 21.53064412772863 28.272162396920425 28.53797725207673 21.659216223274218 68-69 21.597204973406605 28.18643500680487 28.686433980429065 21.52992603935946 70-71 21.54412081719132 28.12523738254055 28.675246313256675 21.655395487011454 72-73 21.58019896240807 28.142398567027477 28.433958412199146 21.843444058365318 74-75 21.473122173988433 28.351635611105923 28.37333568631233 21.801906528593314 76-77 21.57313735771536 28.28900315327287 28.438765657338877 21.699093831672897 78-79 21.444704293599674 28.33933814059971 28.355705558867804 21.860252006932807 80-81 21.354203028299445 28.575520630135976 28.210330057535227 21.859946284029355 82-83 21.54638445293211 28.56095418507808 28.15402170990656 21.738639652083254 84-85 21.569064004534926 28.320615190464345 28.023587773140825 22.08673303185991 86-87 21.60220603251784 28.248525531624484 28.197931280075615 21.95133715578206 88-89 21.660277234633288 28.045552288135156 28.219683556321947 22.074486920909607 90-91 21.412868104393233 28.160524922099196 28.400282865107418 22.02632410840015 92-93 21.551237951354338 28.262249552641993 28.277028916749586 21.909483579254083 94-95 21.400476993453523 28.169751802320125 28.16590425349088 22.263866950735473 96-97 21.406049437565862 28.209896747701375 28.265868256100173 22.118185558632593 98-99 21.30293082718265 28.14072363151005 28.093153432110345 22.463192109196957 100-101 21.32357244431211 28.380161301086805 28.127788244836772 22.16847800976431 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 167.5 2 132.5 3 177.0 4 175.0 5 175.5 6 289.5 7 638.0 8 1115.0 9 1359.0 10 1282.5 11 1213.0 12 1271.5 13 1367.0 14 1491.0 15 1579.0 16 1675.5 17 1751.5 18 1826.0 19 2020.5 20 2234.0 21 2515.5 22 3008.5 23 3614.0 24 4217.0 25 5197.0 26 6825.5 27 8545.0 28 9917.0 29 11499.0 30 13560.0 31 15653.5 32 17972.5 33 20571.0 34 23358.0 35 25676.5 36 28299.5 37 31065.0 38 32186.5 39 32556.0 40 33035.0 41 33880.5 42 34373.0 43 35240.0 44 36505.5 45 36924.5 46 36326.0 47 35720.0 48 37334.5 49 37072.5 50 34531.5 51 32798.0 52 30455.0 53 28636.0 54 27059.0 55 24495.0 56 21988.0 57 19345.0 58 17047.0 59 14887.5 60 12329.0 61 9973.0 62 7714.0 63 5608.0 64 3897.0 65 2819.5 66 1994.5 67 1403.5 68 988.5 69 639.0 70 435.5 71 304.5 72 201.5 73 158.0 74 123.0 75 88.5 76 58.5 77 27.5 78 20.0 79 20.5 80 13.5 81 5.5 82 4.5 83 4.5 84 2.5 85 2.5 86 3.5 87 2.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040623717685679114 2 0.03067295855560115 3 0.02564628641772671 4 0.05375461633155519 5 0.10566270004103406 6 0.13110381616741895 7 0.06975789905621665 8 0.10084119819450144 9 0.08658186294624538 10-11 0.08817193270414443 12-13 0.08386335658596636 14-15 0.07622076323348378 16-17 0.06457734919983586 18-19 0.07560525235945835 20-21 0.06755231842429216 22-23 0.04093147312269183 24-25 0.011745999179318834 26-27 0.003539187525646286 28-29 0.008771029954862536 30-31 0.006565449322938038 32-33 0.00805293393516619 34-35 0.008924907673368896 36-37 0.007950348789495281 38-39 0.005488305293393517 40-41 0.009027492819039803 42-43 0.004308576118178088 44-45 0.002667213787443578 46-47 0.00179524004924087 48-49 3.59048009848174E-4 50-51 0.002667213787443578 52-53 0.007437423061140747 54-55 0.015233894132129668 56-57 0.011951169470660649 58-59 0.005949938448912597 60-61 0.0037443578169881002 62-63 0.01138695116947066 64-65 0.004052113254000821 66-67 0.02559499384489126 68-69 0.051087402544111614 70-71 0.06503898235535495 72-73 0.004411161263848994 74-75 0.015285186704965122 76-77 0.02636438243742306 78-79 0.031083299138284774 80-81 0.0808370947886746 82-83 0.03221173574066476 84-85 0.014618383258104226 86-87 0.03908494050061551 88-89 0.05488305293393517 90-91 0.04965121050471892 92-93 0.04806114074681986 94-95 0.0157468198604842 96-97 0.02020927369716865 98-99 0.04621460812474353 100-101 0.08668444809191629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 974800.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.56588366194296 #Duplication Level Percentage of deduplicated Percentage of total 1 82.83219952735246 55.137985572005896 2 10.726011414089157 14.279728558938624 3 2.9491670916202604 5.889417405612748 4 1.1872700080600014 3.161267089273445 5 0.6164947808122352 2.0518759928871133 6 0.36651526290698866 1.4638447410595827 7 0.2307681512227858 1.0752900135024335 8 0.16505241939309065 0.8789488117954152 9 0.11743205772919313 0.7035271823685635 >10 0.7334108485132791 9.137643405236187 >50 0.051436665269885724 2.3463155885175127 >100 0.02331533611728504 2.4559720912492944 >500 4.632184566509262E-4 0.2035080029102361 >1k 3.0881230443395076E-4 0.6564674821149888 >5k 1.5440615221697538E-4 0.5582080625280263 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5431 0.5571399261386951 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3924 0.4025441116126385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.25266721378744356 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.1292572835453425E-5 0.0 18-19 0.0 0.0 0.0 1.0258514567090685E-4 0.0 20-21 0.0 0.0 0.0 1.5387771850636028E-4 0.0 22-23 0.0 0.0 0.0 4.103405826836274E-4 0.0 24-25 1.0258514567090685E-4 0.0 0.0 6.155108740254411E-4 0.0 26-27 1.0258514567090685E-4 0.0 0.0 0.001077144029544522 0.0 28-29 3.59048009848174E-4 0.0 0.0 0.002154288059089044 0.0 30-31 4.103405826836274E-4 0.0 0.0 0.004616331555190808 0.0 32-33 4.103405826836274E-4 0.0 0.0 0.006514156750102585 0.0 34-35 5.129257283545343E-4 0.0 0.0 0.008976200246204349 0.0 36-37 6.155108740254411E-4 0.0 0.0 0.011027903159622487 0.0 38-39 6.155108740254411E-4 0.0 0.0 0.014310627821091507 0.0 40-41 6.155108740254411E-4 0.0 0.0 0.019029544521953223 0.0 42-43 6.668034468608945E-4 0.0 0.0 0.025851456709068528 0.0 44-45 8.206811653672548E-4 0.0 0.0 0.03580221583914649 0.0 46-47 9.745588838736151E-4 0.0 0.0 0.04903569963069347 0.0 48-49 0.0010258514567090685 0.0 0.0 0.07622076323348378 0.0 50-51 0.0010258514567090685 0.0 0.0 0.09914854329093148 0.0 52-53 0.0010258514567090685 0.0 0.0 0.12223020106688552 0.0 54-55 0.0010258514567090685 0.0 0.0 0.1647517439474764 0.0 56-57 0.0011284366023799754 0.0 0.0 0.21399261386951168 0.0 58-59 0.0011284366023799754 0.0 0.0 0.2735432909314731 0.0 60-61 0.0011284366023799754 0.0 0.0 0.3468916700861715 0.0 62-63 0.0011284366023799754 0.0 0.0 0.4591711120229791 0.0 64-65 0.0011284366023799754 0.0 0.0 0.5501641362330734 0.0 66-67 0.0011284366023799754 0.0 0.0 0.6773697168649979 0.0 68-69 0.0011284366023799754 0.0 0.0 0.8431473122691835 0.0 70-71 0.0011284366023799754 0.0 0.0 0.9397312269183422 0.0 72-73 0.0011284366023799754 0.0 0.0 1.07919573245794 1.0258514567090685E-4 74-75 0.0011284366023799754 0.0 0.0 1.2728251949117766 1.0258514567090685E-4 76-77 0.0011284366023799754 0.0 0.0 1.4108022158391464 1.0258514567090685E-4 78-79 0.0011284366023799754 0.0 0.0 1.5241588018054986 1.0258514567090685E-4 80-81 0.0012310217480508822 0.0 0.0 1.716505949938449 1.0258514567090685E-4 82-83 0.0012310217480508822 0.0 0.0 1.9602482560525236 1.0258514567090685E-4 84-85 0.0013848994665572425 0.0 0.0 2.2492306114074685 1.0258514567090685E-4 86-87 0.001436192039392696 0.0 0.0 2.6760874025441117 1.0258514567090685E-4 88-89 0.001436192039392696 0.0 0.0 3.1321809601969637 1.0258514567090685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 40.58373 1 GTATCAA 5450 0.0 40.480682 1 TATCAAC 6850 0.0 31.934532 2 ATCAACG 6850 0.0 31.934532 3 TCAACGC 6955 0.0 31.38404 4 CAACGCA 6975 0.0 31.157692 5 AACGCAG 7135 0.0 30.522505 6 ACGCAGA 8000 0.0 26.854597 7 CGCAGAG 8040 0.0 26.543638 8 GTACATG 5020 0.0 25.383846 1 GCAGAGT 8330 0.0 24.532906 9 TACATGG 5080 0.0 24.526234 2 ACATGGG 5045 0.0 23.848038 3 GAGTACT 4285 0.0 22.35259 12-13 CATGGGA 3315 0.0 22.09177 4 GTCCACG 135 3.769917E-4 21.132158 1 AGTACTT 4585 0.0 20.78637 12-13 GTGGTAT 1845 0.0 20.35903 1 AGAGTAC 7590 0.0 20.255161 10-11 GTACTTT 4695 0.0 20.199158 14-15 >>END_MODULE