FastQCFastQC Report
Thu 26 May 2016
SRR936892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974800
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.6416700861715223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48140.49384489125974557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.3314526056627No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.12679524004924086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.044.854851
GTATCAA63000.040.5024641
CCGATAG300.003952697431.66490414-15
ATCAACG80250.031.6040973
TCAACGC80300.031.584424
CAACGCA81950.031.0064475
TATCAAC82200.030.9137332
AACGCAG84000.030.2590546
ACGCAGA93350.027.0756057
CGCAGAG93150.026.9834968
GCAGAGT98650.025.1419459
GAGTACT58700.023.7940212-13
GTACATG47550.022.1845261
AGTACTT61350.022.14675112-13
GTACTTT63250.021.8525414-15
TACATGG49650.021.046162
AGAGTAC92100.021.0229410-11
ACATGGG49300.020.5201193
CAGAGTA97950.020.32520710-11
TACTTTT71250.019.06560514-15
Produced by FastQC (version 0.11.5)