##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 974800 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13732868280673 33.0 31.0 34.0 31.0 34.0 2 32.266682396389 33.0 31.0 34.0 31.0 34.0 3 32.38418137053755 34.0 31.0 34.0 31.0 34.0 4 36.06463171932704 37.0 35.0 37.0 35.0 37.0 5 35.905559089043905 37.0 35.0 37.0 35.0 37.0 6 35.999453221173574 37.0 35.0 37.0 35.0 37.0 7 35.960262617972916 37.0 35.0 37.0 35.0 37.0 8 35.916999384489124 37.0 35.0 37.0 35.0 37.0 9 37.6094645055396 39.0 37.0 39.0 35.0 39.0 10-11 37.52448399671727 39.0 37.0 39.0 35.0 39.0 12-13 37.42281955272877 39.0 37.0 39.0 34.0 39.0 14-15 38.60214915880181 40.0 38.0 41.0 34.0 41.0 16-17 38.60375512925728 40.0 38.0 41.0 34.5 41.0 18-19 38.75259283955683 40.0 38.0 41.0 35.0 41.0 20-21 38.769396286417724 40.0 38.0 41.0 35.0 41.0 22-23 38.67710658596635 40.0 38.0 41.0 34.0 41.0 24-25 38.58880693475585 40.0 38.0 41.0 34.0 41.0 26-27 38.39347558473533 40.0 38.0 41.0 34.0 41.0 28-29 38.23042829298318 40.0 38.0 41.0 34.0 41.0 30-31 38.173344275748875 40.0 37.5 41.0 34.0 41.0 32-33 38.01037033237587 40.0 37.0 41.0 33.5 41.0 34-35 37.87217018875667 40.0 37.0 41.0 33.0 41.0 36-37 37.70860484201887 40.0 37.0 41.0 33.0 41.0 38-39 37.53425523184243 40.0 37.0 41.0 33.0 41.0 40-41 37.14139926138695 40.0 36.0 41.0 32.0 41.0 42-43 36.93226764464506 39.0 36.0 41.0 31.0 41.0 44-45 36.65869306524415 39.0 36.0 41.0 31.0 41.0 46-47 36.19536263848995 39.0 35.5 41.0 30.0 41.0 48-49 35.76098276569553 39.0 35.0 40.0 28.5 41.0 50-51 35.46254205990972 39.0 35.0 40.0 27.5 41.0 52-53 35.21068321707017 39.0 35.0 40.0 27.0 41.0 54-55 34.83999384489126 38.0 34.5 40.0 26.0 41.0 56-57 34.648508924907674 38.0 34.0 40.0 26.0 41.0 58-59 34.34192911366434 38.0 34.0 40.0 25.0 41.0 60-61 33.97590069757899 37.5 33.5 40.0 23.5 41.0 62-63 33.61196553139105 37.0 33.0 39.5 23.0 41.0 64-65 33.31371460812474 36.5 33.0 39.0 22.0 40.0 66-67 32.99788007796471 36.0 32.5 39.0 21.5 40.0 68-69 32.56052574887157 36.0 32.0 39.0 20.5 40.0 70-71 32.119985125153875 35.0 31.0 38.0 19.0 40.0 72-73 31.710345199015183 35.0 31.0 37.5 18.0 39.5 74-75 31.230579093147313 35.0 31.0 37.0 15.5 39.0 76-77 29.79252462043496 33.5 29.0 35.5 13.0 38.0 78-79 30.45376846532622 34.0 30.0 36.0 11.0 38.0 80-81 30.313328887977022 34.0 30.0 36.0 7.5 37.5 82-83 29.95491126384899 34.0 30.0 35.0 7.0 37.0 84-85 29.513732047599508 34.0 29.5 35.0 2.0 36.5 86-87 29.1271635207222 34.0 29.0 35.0 2.0 36.0 88-89 28.743227841608537 34.0 29.0 35.0 2.0 36.0 90-91 28.284229072630282 33.5 29.0 35.0 2.0 35.0 92-93 27.800885309807143 33.0 27.0 35.0 2.0 35.0 94-95 27.254059294214198 33.0 26.5 34.5 2.0 35.0 96-97 26.50001846532622 32.0 25.0 34.0 2.0 35.0 98-99 25.650419573245795 32.0 24.0 34.0 2.0 35.0 100-101 22.550821707016823 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1792858945587099 1101 2 0.29243723570008484 1101 3 0.2046322094552835 1101 4 0.13737258323292423 1101 5 -0.03291638727432655 1101 6 0.07508295328221237 1101 7 0.04547430435903976 1101 8 0.19153431778770624 1101 9 0.2784755268607597 1101 10-11 0.35925165736507836 1101 12-13 0.2933764445909688 1101 14-15 0.4562091054937625 1101 16-17 0.44246611176259876 1101 18-19 0.21243141870255755 1101 20-21 0.07783449574282741 1101 22-23 0.21297194615440418 1101 24-25 0.21194362981561454 1101 26-27 0.31701221001161173 1101 28-29 0.2754508985212496 1101 30-31 0.2885082418502094 1101 32-33 0.3859351945441887 1101 34-35 0.2011476380903403 1101 36-37 0.27712841082720274 1101 38-39 0.28013400469641425 1101 40-41 0.2189846044189423 1101 42-43 0.289172580745344 1101 44-45 0.06082229828313501 1101 46-47 0.019637779822126333 1101 48-49 0.18844840044230438 1101 50-51 0.3074909141852302 1101 52-53 0.6031049299489553 1101 54-55 0.25239030351428227 1101 56-57 0.23513252559146736 1101 58-59 0.410840394284655 1101 60-61 0.4272978458840839 1101 62-63 0.5230542339451958 1101 64-65 0.4709494164680308 1101 66-67 0.3799820240262832 1101 68-69 0.043124295467542595 1101 70-71 0.1226558779924325 1101 72-73 0.16319717966540281 1101 74-75 0.3337731546628184 1101 76-77 0.18585888970065056 1101 78-79 0.3175734313943721 1101 80-81 0.29066512755304075 1101 82-83 0.5384549921715198 1101 84-85 0.251797288010021 1101 86-87 -0.027761851733753673 1101 88-89 0.08219329289216049 1101 90-91 0.27259510617993143 1101 92-93 0.20409536660040217 1101 94-95 0.10448155246021784 1101 96-97 0.17364983039780313 1101 98-99 0.30077289303844523 1101 100-101 0.24225850436219432 1102 1 -0.06410449269912988 1102 2 0.03187644582243365 1102 3 0.18531012590812423 1102 4 0.10725072732007845 1102 5 0.06506810988975076 1102 6 0.16595611423510093 1102 7 0.14141668917506678 1102 8 0.10595343102012578 1102 9 0.2772797758855461 1102 10-11 0.2722771253514864 1102 12-13 0.2877470793172776 1102 14-15 0.3632345757162554 1102 16-17 0.32612300781891435 1102 18-19 0.29351663957827867 1102 20-21 0.3333050983811887 1102 22-23 0.015326161246470349 1102 24-25 0.23972904757936675 1102 26-27 0.17306676845582558 1102 28-29 0.0997794835101331 1102 30-31 0.21296601713846997 1102 32-33 0.006417027961937549 1102 34-35 0.3740762049668618 1102 36-37 0.24703310852413551 1102 38-39 0.411376758356063 1102 40-41 0.5168534955513806 1102 42-43 0.4212938396212351 1102 44-45 0.5410985564490787 1102 46-47 0.5681299820052246 1102 48-49 0.574463536558909 1102 50-51 0.5059716004870367 1102 52-53 0.49240572588642095 1102 54-55 0.879004064209802 1102 56-57 0.5938284694297451 1102 58-59 0.5665560727657137 1102 60-61 0.7077196178316214 1102 62-63 0.504338970259461 1102 64-65 0.7030849863420414 1102 66-67 0.5605535583329839 1102 68-69 0.512371581688619 1102 70-71 0.3126134145777151 1102 72-73 0.12832614076520343 1102 74-75 0.358672893822515 1102 76-77 0.37342240873036303 1102 78-79 0.12811063048606997 1102 80-81 0.5213340996320603 1102 82-83 0.6417873863456016 1102 84-85 0.2027519322084217 1102 86-87 0.030026070454663767 1102 88-89 0.26881932974074374 1102 90-91 0.2880996191755507 1102 92-93 0.23861226419962023 1102 94-95 0.27498535549615966 1102 96-97 0.3073324415288532 1102 98-99 0.0939711439661437 1102 100-101 0.2893075877315816 1103 1 0.14011019915838574 1103 2 0.23415184528895594 1103 3 0.19389620444835742 1103 4 0.1814851777966453 1103 5 0.030383588435078934 1103 6 0.08204625697810286 1103 7 0.05898512680354884 1103 8 0.09883068710978193 1103 9 0.09646347785065501 1103 10-11 0.1621438793417198 1103 12-13 0.19497382470813562 1103 14-15 0.25447462576108393 1103 16-17 0.23671110100690385 1103 18-19 0.22632957765058137 1103 20-21 0.08627055348878798 1103 22-23 0.06119402783645711 1103 24-25 0.18092388440730645 1103 26-27 0.18232633553927258 1103 28-29 0.11258629341847382 1103 30-31 0.3845945019083672 1103 32-33 0.3049577935502441 1103 34-35 0.34936020048543526 1103 36-37 0.25876056369600064 1103 38-39 0.3605697910879968 1103 40-41 0.4301119943617593 1103 42-43 0.27125826745795223 1103 44-45 0.22889187442321202 1103 46-47 0.3204324761568671 1103 48-49 0.2300247068276633 1103 50-51 0.27747835310314173 1103 52-53 0.0886126361947035 1103 54-55 0.39909236296277584 1103 56-57 0.2486696081379094 1103 58-59 0.09271020107595263 1103 60-61 0.05362261331806195 1103 62-63 0.38259989041491593 1103 64-65 0.29575241099499294 1103 66-67 0.3008537718767812 1103 68-69 0.13662010588711127 1103 70-71 0.23740322298670335 1103 72-73 0.33986803335942994 1103 74-75 0.1673475703208389 1103 76-77 0.26054644919619463 1103 78-79 0.3455147269365 1103 80-81 0.3971925135568455 1103 82-83 0.7029510061068791 1103 84-85 0.2534292439280641 1103 86-87 0.18272927782264858 1103 88-89 0.47300105362761613 1103 90-91 0.08580371861803116 1103 92-93 0.0714402733442121 1103 94-95 0.2064396807276836 1103 96-97 0.4758472538441332 1103 98-99 0.18605290231701233 1103 100-101 0.7815966841432278 1104 1 0.07269528711903206 1104 2 0.0490379900720086 1104 3 -0.06893374910324468 1104 4 -0.017595564730342517 1104 5 -0.19442988969209551 1104 6 -0.0173322731809904 1104 7 0.04069708200592714 1104 8 0.04424168259441075 1104 9 0.03281946354390186 1104 10-11 0.11673531147365424 1104 12-13 0.10678660691855413 1104 14-15 0.12475898058152524 1104 16-17 -0.018686299824963726 1104 18-19 0.1400278087273108 1104 20-21 0.2605370086830803 1104 22-23 0.03258456660549314 1104 24-25 0.15052203294506938 1104 26-27 0.2266829362287197 1104 28-29 0.03331652048154865 1104 30-31 0.20616063827569064 1104 32-33 0.11458814846778154 1104 34-35 0.08016179947554036 1104 36-37 0.023330309119948822 1104 38-39 -0.003924698222398604 1104 40-41 0.07945087345026991 1104 42-43 -0.10135659926590534 1104 44-45 0.07657511901367542 1104 46-47 -0.03991888088079065 1104 48-49 0.11367309084423738 1104 50-51 0.1442588480132443 1104 52-53 -0.023203382837813535 1104 54-55 0.114769910746368 1104 56-57 -0.03433383907761112 1104 58-59 -0.35711501053129524 1104 60-61 -0.340593994816075 1104 62-63 -0.1653900430683919 1104 64-65 -0.025121854651004583 1104 66-67 -0.3407895626973314 1104 68-69 -0.14902944338542312 1104 70-71 -0.18304378214365968 1104 72-73 0.0026680463067201288 1104 74-75 -0.054346420518953664 1104 76-77 -0.1238383118454145 1104 78-79 0.18596772018772967 1104 80-81 0.23808652381757156 1104 82-83 0.12729838185337172 1104 84-85 0.2119250927037335 1104 86-87 0.08049425217082984 1104 88-89 0.021516970700236726 1104 90-91 0.07320438864020318 1104 92-93 0.24167283901124037 1104 94-95 0.017939300401089042 1104 96-97 -0.04322976633745057 1104 98-99 0.0348690346383016 1104 100-101 0.8238695806134722 1105 1 -0.026034170375297094 1105 2 0.1254758499413242 1105 3 -0.003036652087565983 1105 4 0.09650702137753342 1105 5 0.03296988823033331 1105 6 0.08359023336448956 1105 7 0.10304450269769916 1105 8 0.19209293022939278 1105 9 0.14734719221316794 1105 10-11 0.06580204433228687 1105 12-13 0.13263415238042597 1105 14-15 0.25765299993475566 1105 16-17 0.064746937609236 1105 18-19 -0.0057611013893676954 1105 20-21 0.14121982460559224 1105 22-23 0.15858190220273372 1105 24-25 0.05372915263062339 1105 26-27 -0.027896918615461175 1105 28-29 -0.05366243232102619 1105 30-31 0.0249347093325909 1105 32-33 0.17181042276923364 1105 34-35 0.004742565339505234 1105 36-37 0.019077893267152035 1105 38-39 0.028844947126842158 1105 40-41 0.0916278410219249 1105 42-43 0.08598636535088389 1105 44-45 0.17226454313034623 1105 46-47 0.07208142807373719 1105 48-49 0.14581327434210323 1105 50-51 0.08919529448159125 1105 52-53 0.021092313055866896 1105 54-55 0.2595560389519349 1105 56-57 0.005304433259205155 1105 58-59 -0.01947457815338538 1105 60-61 0.2688524880534544 1105 62-63 0.13328052822023295 1105 64-65 0.05256931756738936 1105 66-67 -0.056826349411764454 1105 68-69 0.08714814209530886 1105 70-71 0.05520967412013178 1105 72-73 -0.13430674749253768 1105 74-75 -0.027879975753815955 1105 76-77 -0.1637581421052694 1105 78-79 -0.07699846777545716 1105 80-81 -0.2481824937202184 1105 82-83 0.11779076823666301 1105 84-85 -0.26603582058698905 1105 86-87 -0.03371726021442001 1105 88-89 0.08793625368806701 1105 90-91 0.029116312832794478 1105 92-93 0.1365919893070604 1105 94-95 0.4091434044791562 1105 96-97 0.44616147440546783 1105 98-99 0.6599325749667706 1105 100-101 0.8277199594788733 1106 1 -0.0601850947162319 1106 2 0.02198683739018037 1106 3 -0.035889094464245375 1106 4 0.0586439662446665 1106 5 3.59319945033576E-4 1106 6 0.07920849324589341 1106 7 0.017940467134565097 1106 8 0.11421286752884185 1106 9 0.10484524661192296 1106 10-11 0.11347203301937014 1106 12-13 0.2036034055156648 1106 14-15 0.31915691362728893 1106 16-17 0.2577985314746343 1106 18-19 0.12429120282204309 1106 20-21 0.07817597919793684 1106 22-23 0.15214253494536223 1106 24-25 0.06052326855535739 1106 26-27 0.35617410240721625 1106 28-29 0.26762189364377775 1106 30-31 0.17938234953257393 1106 32-33 0.27883758650272483 1106 34-35 0.19683430096857535 1106 36-37 0.06950609527699214 1106 38-39 0.220994393065439 1106 40-41 0.2175631935937261 1106 42-43 0.37652637259861876 1106 44-45 0.12522172971880963 1106 46-47 0.005206636531013942 1106 48-49 -0.1181040335738004 1106 50-51 -0.01875735590680705 1106 52-53 0.1118999094299582 1106 54-55 0.3950411747281706 1106 56-57 -0.08534276240165894 1106 58-59 0.20064739428315193 1106 60-61 -0.11780870744955507 1106 62-63 0.028825716665600964 1106 64-65 0.13586905727692056 1106 66-67 -0.0926097116992608 1106 68-69 -0.07101926702581096 1106 70-71 -0.08782575237178847 1106 72-73 0.056087201751246596 1106 74-75 0.03366467462778999 1106 76-77 0.09507302044345778 1106 78-79 0.2136616278369452 1106 80-81 0.05993963826835014 1106 82-83 0.3444267918605455 1106 84-85 0.2764987992336181 1106 86-87 -0.07070405219166531 1106 88-89 -0.08203588411396723 1106 90-91 -0.3641976932047193 1106 92-93 -0.13077732866740988 1106 94-95 0.14299533849590418 1106 96-97 0.36278068565829713 1106 98-99 -0.07680360150456877 1106 100-101 0.46669147689507895 1107 1 0.2477905364741062 1107 2 0.24753089015041496 1107 3 0.2769525277003595 1107 4 0.17002921948076022 1107 5 0.11127831570871649 1107 6 0.21322894686413463 1107 7 0.19675935010508283 1107 8 0.14215517502335473 1107 9 0.35943096884818004 1107 10-11 0.278825445898363 1107 12-13 0.2992787531803245 1107 14-15 0.28212087123899465 1107 16-17 0.3272137554667722 1107 18-19 0.342431132632008 1107 20-21 0.18851729566677022 1107 22-23 0.08641223060950409 1107 24-25 0.27826950792600513 1107 26-27 0.3769671286581868 1107 28-29 0.32558981101563234 1107 30-31 0.20582417648476792 1107 32-33 0.22803266382946674 1107 34-35 0.25864655691192695 1107 36-37 0.41499716838913514 1107 38-39 0.4407302262092614 1107 40-41 0.322260280513035 1107 42-43 0.1498932429481883 1107 44-45 0.2212679120643415 1107 46-47 0.0019548334973720216 1107 48-49 0.10924219574030758 1107 50-51 -0.2055311856062687 1107 52-53 -0.18020125472809667 1107 54-55 -0.16338042944895648 1107 56-57 -0.3345630953415011 1107 58-59 -0.14704640254972645 1107 60-61 -0.01737021972366648 1107 62-63 0.15822130756124153 1107 64-65 0.21975254776263853 1107 66-67 0.13792889567651656 1107 68-69 0.021808253135823463 1107 70-71 -0.09991416301662781 1107 72-73 0.0568357278405216 1107 74-75 -0.046016213272711326 1107 76-77 -0.034752830105023946 1107 78-79 0.09619731424679756 1107 80-81 -0.11321558775795992 1107 82-83 0.3388488440105313 1107 84-85 0.2535339045358107 1107 86-87 0.33273836786746 1107 88-89 0.16803296885836616 1107 90-91 0.0692006266684757 1107 92-93 0.26009956678920787 1107 94-95 0.4187906331809117 1107 96-97 0.15323280684409823 1107 98-99 0.436593301311607 1107 100-101 0.5000408854451877 1108 1 -0.007470787407946489 1108 2 0.057135156063914394 1108 3 -0.012687363909492433 1108 4 -0.0017936974247092508 1108 5 -0.11744484466193938 1108 6 -0.010722341759027643 1108 7 -0.0520691742941608 1108 8 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-1.4879774297227044 2316 88-89 -2.1447825752185707 2316 90-91 -2.1288415257717297 2316 92-93 -1.836781421493889 2316 94-95 -1.7903476374251213 2316 96-97 -1.5907676547807945 2316 98-99 -1.7432495433123059 2316 100-101 -2.581680036942224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 13.0 6 60.0 7 120.0 8 262.0 9 580.0 10 996.0 11 1688.0 12 2627.0 13 3785.0 14 4707.0 15 6995.0 16 9209.0 17 13440.0 18 11442.0 19 6125.0 20 4978.0 21 5052.0 22 5407.0 23 5822.0 24 6655.0 25 7960.0 26 9917.0 27 11957.0 28 15080.0 29 18774.0 30 23525.0 31 29823.0 32 37990.0 33 49755.0 34 67579.0 35 96285.0 36 148502.0 37 217446.0 38 142965.0 39 7271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36649719630004 20.303712786205693 15.811020875123768 21.518769142370502 2 15.251714275162195 21.618640972715223 40.44283902843378 22.6868057236888 3 18.754815114972892 26.363491811098744 31.107771377571698 23.77392169635667 4 12.501846532622077 18.17880590890439 38.639515798112434 30.679831760361097 5 12.860493077383934 38.19020985334067 34.38359171748176 14.565705351793643 6 29.53693065244153 38.17890849405006 17.988100123102175 14.296060730406237 7 26.504055199128434 34.32865065377648 21.668078925069604 17.499215222025484 8 25.76528518670497 34.80837094788674 20.494768157570782 18.931575707837506 9 26.483408374444373 16.6487655403832 21.094399973738177 35.773426111434254 10-11 23.883309396799344 27.65464710709889 28.02267131719327 20.439372178908492 12-13 25.539718545101625 25.202237358177364 28.594541412363128 20.66350268435788 14-15 22.567760269194466 25.97465017029833 27.713539743116257 23.744049817390948 16-17 20.798368896183835 29.396542880590893 28.194911776774724 21.610176446450556 18-19 20.668869182171218 27.211735396537424 31.812807725115395 20.306587696175963 20-21 21.64421139127986 26.21714739438617 31.999472706671543 20.139168507662422 22-23 21.251929932548535 26.311353902182553 31.767741273627248 20.66897489164166 24-25 20.957667040078128 26.418091580187035 32.03854960422488 20.585691775509954 26-27 20.97063086851751 26.737512040943823 31.72346299083196 20.56839409970671 28-29 20.729072630283135 26.692911366434142 31.95034878949528 20.627667213787444 30-31 20.860484201887566 26.642849815346736 31.896337710299548 20.600328272466147 32-33 20.784519901518262 26.657109150594994 31.667572835453427 20.89079811243332 34-35 21.104534263438655 26.641567501025854 31.621563397620022 20.63233483791547 36-37 21.227305718484473 26.476965256173845 31.500008976407436 20.795720048934243 38-39 21.040768200465067 26.74153464653247 31.643844962544637 20.57385219045782 40-41 21.24322486100931 26.598399356583 31.454392228934168 20.703983553473524 42-43 21.0475995075913 26.784622486663935 31.194193680755028 20.97358432498974 44-45 21.240013849172257 26.923457676673124 30.71905415282818 21.117474321326444 46-47 21.380491845742323 27.13630266799002 30.140516363655017 21.342689122612647 48-49 21.53621255642183 27.50907878539188 29.71014567090685 21.24456298727944 50-51 21.55987632030333 27.58454250002052 29.47492552135055 21.380655658325605 52-53 21.693223464360546 27.75166124246318 29.225351932067944 21.329763361108334 54-55 21.476815757078374 27.72599507591301 29.310166187935987 21.48702297907263 56-57 21.645931590920164 27.97745172715359 28.95898891821122 21.41762776371503 58-59 21.622959388058774 27.5758245670007 29.172665763574972 21.628550281365552 60-61 21.564031120996002 27.68097491115908 29.452304939801554 21.302689028043364 62-63 21.55221583914649 27.55503693065244 29.607355354944602 21.28539187525646 64-65 21.478457119409107 27.426600328272464 29.540572425112842 21.55437012720558 66-67 21.36141842501971 27.889068469977673 29.24462928017505 21.504883824827566 68-69 21.604893311448503 27.55796060730406 29.29852277390234 21.538623307345098 70-71 21.57690267819864 27.62446950493282 29.24649952759395 21.552128289274588 72-73 21.507060177051976 27.671872699845146 29.014611737707845 21.806455385395033 74-75 21.43693973733183 27.818368716184196 28.841075704322883 21.90361584216109 76-77 21.529082888797703 27.81026877308166 28.928805908904394 21.73184242921625 78-79 21.376982012179955 27.860578365682752 28.88858569232513 21.873853929812164 80-81 21.303129718994157 28.024618787341605 28.76108963626814 21.9111618573961 82-83 21.503817710112546 28.01587814513556 28.67478321171852 21.805520933033375 84-85 21.412926840778105 27.850854245787243 28.58430329777652 22.15191561565813 86-87 21.4064349742203 27.63736692114674 28.58525561193191 22.37094249270105 88-89 21.460362761939596 27.614933345917482 28.621784695182413 22.30291919696052 90-91 21.20115841047863 27.70906169982273 28.896760718271945 22.193019171426695 92-93 21.18219121871153 27.579144439885106 28.738459171112023 22.500205170291345 94-95 21.070475995075913 27.616023799753798 28.719788674599915 22.593711530570374 96-97 21.269923338041995 27.561835824616153 28.704738104983697 22.463502732358158 98-99 20.93848353911998 27.44004899486662 28.61039643166365 23.01107103434975 100-101 20.68861338654165 27.754222726037025 28.560964725554864 22.996199161866464 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 360.0 1 265.0 2 184.0 3 202.0 4 195.5 5 212.0 6 321.0 7 662.0 8 1178.5 9 1494.5 10 1421.5 11 1318.0 12 1389.5 13 1524.0 14 1653.5 15 1778.0 16 1908.0 17 2009.0 18 2119.5 19 2326.0 20 2593.5 21 2921.5 22 3384.0 23 3977.0 24 4576.5 25 5557.5 26 7109.5 27 8821.5 28 10262.5 29 11858.5 30 13772.0 31 15804.5 32 18156.5 33 20442.0 34 22985.0 35 25346.0 36 28232.0 37 30720.0 38 31753.0 39 32394.5 40 33027.5 41 33835.0 42 34210.0 43 34969.5 44 36047.0 45 36862.5 46 36372.5 47 35406.5 48 36706.5 49 36525.5 50 34207.0 51 32711.0 52 30440.0 53 28409.0 54 26707.0 55 24122.0 56 21516.5 57 18894.0 58 16811.5 59 14857.0 60 12489.0 61 9941.5 62 7656.5 63 5717.5 64 3845.0 65 2703.5 66 2025.5 67 1483.0 68 1023.5 69 667.5 70 466.5 71 327.0 72 231.0 73 178.5 74 138.0 75 103.0 76 80.5 77 38.0 78 13.5 79 8.0 80 6.5 81 4.5 82 2.0 83 1.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020004103405826836 2 0.004513746409519901 3 5.129257283545343E-4 4 0.0 5 0.0024620434961017644 6 0.0 7 2.051702913418137E-4 8 0.0 9 1.0258514567090685E-4 10-11 0.0 12-13 0.008104226508001642 14-15 0.004103405826836274 16-17 0.0 18-19 5.642183011899877E-4 20-21 0.001077144029544522 22-23 0.0025646286417726713 24-25 4.103405826836274E-4 26-27 1.0258514567090685E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.002308165777595404 38-39 0.010925318013951581 40-41 4.616331555190808E-4 42-43 0.0 44-45 0.0012823143208863356 46-47 2.564628641772671E-4 48-49 0.0 50-51 0.0024620434961017644 52-53 2.564628641772671E-4 54-55 0.0 56-57 2.564628641772671E-4 58-59 5.1292572835453425E-5 60-61 0.0024620434961017644 62-63 0.0 64-65 0.0 66-67 5.1292572835453425E-5 68-69 0.0 70-71 3.0775543701272055E-4 72-73 1.5387771850636028E-4 74-75 0.003487894952810833 76-77 0.0 78-79 1.5387771850636028E-4 80-81 0.0016413623307345096 82-83 1.0258514567090685E-4 84-85 0.0022568732047599507 86-87 2.051702913418137E-4 88-89 0.0029236766516208454 90-91 0.0016413623307345096 92-93 0.0 94-95 0.0 96-97 1.0258514567090685E-4 98-99 4.103405826836274E-4 100-101 0.0015387771850636028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 974800.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.57483799074905 #Duplication Level Percentage of deduplicated Percentage of total 1 81.85016709418977 52.85461279623044 2 11.13832760238951 14.385114008243816 3 3.222679567504469 6.2431203290309485 4 1.277821709224951 3.3006051941705303 5 0.6651600289246573 2.1476300552865846 6 0.3961047427929198 1.534703975593207 7 0.26697360058013736 1.206784390368852 8 0.1795934504521489 0.9277774373717705 9 0.12877073761993774 0.748381456778106 >10 0.7875764348795004 9.38941197918385 >50 0.06017749455879532 2.629922258713629 >100 0.0256901405145241 2.900872087648485 >500 3.191321229241998E-4 0.13024196045619577 >1k 4.7869818438629974E-4 0.9563098378116716 >5k 1.595660614620999E-4 0.6445122331119497 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6255 0.6416700861715223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4814 0.49384489125974557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3231 0.3314526056627 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1236 0.12679524004924086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.1292572835453425E-5 0.0 18-19 5.1292572835453425E-5 0.0 0.0 1.0258514567090685E-4 0.0 20-21 1.0258514567090685E-4 0.0 0.0 1.5387771850636028E-4 0.0 22-23 1.0258514567090685E-4 0.0 0.0 3.0775543701272055E-4 0.0 24-25 1.0258514567090685E-4 0.0 0.0 5.129257283545343E-4 0.0 26-27 1.0258514567090685E-4 0.0 0.0 9.745588838736151E-4 0.0 28-29 1.0258514567090685E-4 0.0 0.0 0.0022055806319244975 0.0 30-31 1.0258514567090685E-4 0.0 0.0 0.004513746409519901 0.0 32-33 1.0258514567090685E-4 0.0 0.0 0.006411571604431678 0.0 34-35 1.0258514567090685E-4 0.0 0.0 0.008976200246204349 0.0 36-37 1.0258514567090685E-4 0.0 0.0 0.011130488305293392 0.0 38-39 2.051702913418137E-4 0.0 0.0 0.014413212966762411 0.0 40-41 2.051702913418137E-4 0.0 0.0 0.01918342224045958 0.0 42-43 2.564628641772671E-4 0.0 0.0 0.02610791957324579 0.0 44-45 4.103405826836274E-4 0.0 0.0 0.035904800984817395 0.0 46-47 4.616331555190808E-4 0.0 0.0 0.049189577349199834 0.0 48-49 5.129257283545343E-4 0.0 0.0 0.07647722609766106 0.0 50-51 5.129257283545343E-4 0.0 0.0 0.09909725071809602 0.0 52-53 5.642183011899877E-4 0.0 0.0 0.1219224456298728 0.0 54-55 6.155108740254411E-4 0.0 0.0 0.16444398851046368 0.0 56-57 6.155108740254411E-4 0.0 0.0 0.21383873615100535 0.0 58-59 6.155108740254411E-4 0.0 0.0 0.2732868280672959 0.0 60-61 6.155108740254411E-4 0.0 0.0 0.3468916700861715 0.0 62-63 6.155108740254411E-4 0.0 0.0 0.45911981945014363 0.0 64-65 6.155108740254411E-4 0.0 0.0 0.5503693065244153 0.0 66-67 6.155108740254411E-4 0.0 0.0 0.6775748871563398 0.0 68-69 6.155108740254411E-4 0.0 0.0 0.8454041854739434 0.0 70-71 6.155108740254411E-4 0.0 0.0 0.9422445629872795 0.0 72-73 6.155108740254411E-4 0.0 0.0 1.0825297496922446 0.0 74-75 6.155108740254411E-4 0.0 0.0 1.2767234304472712 0.0 76-77 6.155108740254411E-4 0.0 0.0 1.414803036520312 0.0 78-79 6.155108740254411E-4 0.0 0.0 1.528826425933525 0.0 80-81 6.155108740254411E-4 0.0 0.0 1.7212248666393106 0.0 82-83 6.155108740254411E-4 0.0 0.0 1.9641977841608536 0.0 84-85 6.155108740254411E-4 0.0 0.0 2.2530775543701274 0.0 86-87 6.155108740254411E-4 0.0 0.0 2.6792675420599097 0.0 88-89 6.155108740254411E-4 0.0 0.0 3.13289905621666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3570 0.0 44.85485 1 GTATCAA 6300 0.0 40.502464 1 CCGATAG 30 0.0039526974 31.664904 14-15 ATCAACG 8025 0.0 31.604097 3 TCAACGC 8030 0.0 31.58442 4 CAACGCA 8195 0.0 31.006447 5 TATCAAC 8220 0.0 30.913733 2 AACGCAG 8400 0.0 30.259054 6 ACGCAGA 9335 0.0 27.075605 7 CGCAGAG 9315 0.0 26.983496 8 GCAGAGT 9865 0.0 25.141945 9 GAGTACT 5870 0.0 23.79402 12-13 GTACATG 4755 0.0 22.184526 1 AGTACTT 6135 0.0 22.146751 12-13 GTACTTT 6325 0.0 21.85254 14-15 TACATGG 4965 0.0 21.04616 2 AGAGTAC 9210 0.0 21.02294 10-11 ACATGGG 4930 0.0 20.520119 3 CAGAGTA 9795 0.0 20.325207 10-11 TACTTTT 7125 0.0 19.065605 14-15 >>END_MODULE