FastQCFastQC Report
Thu 26 May 2016
SRR936891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010928
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63740.31696808637604135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.23546342783033503No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35550.17678405194019875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.141427241552159No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22380.11129190105264833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137900.025.7138921
GTACATG102250.024.2661271
TACATGG101700.023.509332
GAGTACT56150.023.1434312-13
ACATGGG102700.022.3056953
TCAACGC157700.022.0606634
TATCAAC159150.021.9818612
CAACGCA158150.021.9651055
ATCAACG159200.021.8528063
GGTATCA100950.021.7064041
AACGCAG161550.021.5616576
GTACTTT64000.020.12014814-15
GGACCGA2601.48411345E-820.1050176
AGTACTT60500.019.90869312-13
ACGCAGA175950.019.4724357
CGCAGAG177000.019.1704228
CATGGGA61000.018.9328334
AGAGTAC126300.018.9058510-11
CATGGGG43700.018.7062264
GCAGAGT182600.018.2185949
Produced by FastQC (version 0.11.5)