##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936891_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2010928 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71914708035295 33.0 31.0 34.0 30.0 34.0 2 31.815020726749044 33.0 31.0 34.0 30.0 34.0 3 31.830437986839907 33.0 31.0 34.0 30.0 34.0 4 35.39935641654002 37.0 35.0 37.0 33.0 37.0 5 35.43270320966241 37.0 35.0 37.0 33.0 37.0 6 35.483926823834565 37.0 35.0 37.0 33.0 37.0 7 35.42467159440815 37.0 35.0 37.0 33.0 37.0 8 35.3919866847545 37.0 35.0 37.0 33.0 37.0 9 36.99911433925033 39.0 37.0 39.0 33.0 39.0 10-11 36.86898834766834 39.0 37.0 39.0 33.0 39.0 12-13 36.76123287357876 39.0 37.0 39.0 32.5 39.0 14-15 37.904141272089305 40.0 38.0 41.0 33.0 41.0 16-17 37.83794919559527 40.0 38.0 41.0 33.0 41.0 18-19 37.79441108781617 40.0 38.0 41.0 32.0 41.0 20-21 37.72946520213553 40.0 37.5 41.0 32.0 41.0 22-23 37.59119396616885 40.0 37.0 41.0 32.0 41.0 24-25 37.42798424409029 40.0 37.0 41.0 32.0 41.0 26-27 37.39527447029431 40.0 37.0 41.0 32.0 41.0 28-29 37.47217379239834 40.0 37.0 41.0 32.0 41.0 30-31 37.359492731713914 40.0 37.0 41.0 31.5 41.0 32-33 37.197360372922354 40.0 36.5 41.0 31.0 41.0 34-35 37.018421345766725 39.5 36.0 41.0 31.0 41.0 36-37 36.88288218175887 39.0 36.0 41.0 30.0 41.0 38-39 36.7299779007503 39.0 36.0 41.0 30.0 41.0 40-41 36.53553956183414 39.0 35.5 41.0 30.0 41.0 42-43 36.33841564690531 39.0 35.0 41.0 30.0 41.0 44-45 36.11240208500752 39.0 35.0 40.5 29.5 41.0 46-47 35.8028678799042 39.0 35.0 40.0 28.0 41.0 48-49 35.49547099647526 39.0 35.0 40.0 27.0 41.0 50-51 34.84584281485961 38.5 34.0 39.5 26.0 40.5 52-53 35.15685618778991 38.5 34.5 40.0 27.0 40.5 54-55 35.47204822847959 39.0 35.0 40.5 27.0 41.0 56-57 35.35886913902437 39.0 35.0 41.0 27.0 41.0 58-59 35.122196567952706 38.5 34.5 40.0 26.0 41.0 60-61 34.83412906876825 38.0 34.0 40.0 26.0 41.0 62-63 34.51531208476882 38.0 34.0 40.0 26.0 41.0 64-65 34.207822458089 37.0 34.0 40.0 25.5 41.0 66-67 33.83326429389814 37.0 33.5 39.5 24.5 41.0 68-69 33.39246158987294 36.0 33.0 39.0 23.0 41.0 70-71 33.035800138045715 36.0 33.0 39.0 22.5 40.5 72-73 32.66172831647876 35.0 32.0 38.5 22.0 40.0 74-75 32.233785595506156 35.0 32.0 37.0 21.5 39.5 76-77 31.79060761996451 35.0 31.5 37.0 20.0 39.0 78-79 31.371534435842555 35.0 31.0 36.0 18.5 39.0 80-81 30.953868562176268 35.0 31.0 36.0 18.0 37.5 82-83 30.55110849319319 34.5 31.0 35.5 14.5 37.0 84-85 30.211124913472787 34.0 30.0 35.0 9.5 37.0 86-87 29.892897458287916 34.0 30.0 35.0 7.0 36.0 88-89 29.533184181631565 34.0 30.0 35.0 4.5 36.0 90-91 29.211136599619678 34.0 29.5 35.0 2.0 36.0 92-93 28.873176463801787 34.0 29.0 35.0 2.0 35.0 94-95 28.492385356412562 34.0 29.0 35.0 2.0 35.0 96-97 28.065723387411186 34.0 29.0 35.0 2.0 35.0 98-99 27.490310195094004 34.0 28.0 35.0 2.0 35.0 100-101 25.34205923832181 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5381613455838732 1101 2 0.3987947653616324 1101 3 0.45079761149192166 1101 4 0.32662180853508715 1101 5 0.3061884019576908 1101 6 0.33221463505556414 1101 7 0.3813489766783036 1101 8 0.32474534405123023 1101 9 0.4193321297428767 1101 10-11 0.4230020227648197 1101 12-13 0.534995278194657 1101 14-15 0.7139620984648687 1101 16-17 0.6532182753242921 1101 18-19 0.8956071160930392 1101 20-21 0.8720717150895965 1101 22-23 0.8736173308962165 1101 24-25 0.8559845375623993 1101 26-27 0.7994253344002971 1101 28-29 0.8535339837489246 1101 30-31 0.7745776452355955 1101 32-33 0.744117969018042 1101 34-35 0.7220118606965684 1101 36-37 0.6615231886291824 1101 38-39 0.6528207932714309 1101 40-41 0.7632132339777087 1101 42-43 0.6898299908701802 1101 44-45 0.7508005128275457 1101 46-47 0.6878331191914953 1101 48-49 0.5971122250942713 1101 50-51 0.6916366766366835 1101 52-53 0.7919157487099611 1101 54-55 0.6716104445091702 1101 56-57 0.6576537189266318 1101 58-59 0.5922270192677601 1101 60-61 0.6934525072438404 1101 62-63 0.5215205351896159 1101 64-65 0.6056971641769238 1101 66-67 0.6147490034723333 1101 68-69 0.5937870211740233 1101 70-71 0.7067077740508338 1101 72-73 0.7460150667989254 1101 74-75 0.5981135865583127 1101 76-77 0.6062251829526382 1101 78-79 0.5779346487570294 1101 80-81 0.5967973442839636 1101 82-83 0.404861785250727 1101 84-85 0.7129729816226487 1101 86-87 0.7990348899831261 1101 88-89 0.8815147042784375 1101 90-91 0.8114505005771946 1101 92-93 0.6489643782941528 1101 94-95 0.9346814957891887 1101 96-97 0.8057471834879699 1101 98-99 0.8188174251945171 1101 100-101 1.1173017257753628 1102 1 0.6478246691775382 1102 2 0.6458501330637638 1102 3 0.6013358055624565 1102 4 0.5191081502751587 1102 5 0.49062157519683325 1102 6 0.5310598749455764 1102 7 0.4954124974582399 1102 8 0.37053311993851423 1102 9 0.4543787580730907 1102 10-11 0.6372641058080006 1102 12-13 0.6602393679909824 1102 14-15 0.8121370486298716 1102 16-17 0.8026218706234047 1102 18-19 0.7863894956813908 1102 20-21 0.7328729278808552 1102 22-23 0.7160866820720386 1102 24-25 0.7832137713836715 1102 26-27 0.8586096317686795 1102 28-29 0.9279508604046001 1102 30-31 0.8303031064336892 1102 32-33 1.044211716251091 1102 34-35 0.8655940938310422 1102 36-37 0.7916753977614945 1102 38-39 0.8656276105558689 1102 40-41 0.9324730556007097 1102 42-43 0.9395538942010475 1102 44-45 0.9639734648064646 1102 46-47 0.8295625432592288 1102 48-49 1.0079907329877074 1102 50-51 0.8212405811162071 1102 52-53 1.0316765407095616 1102 54-55 1.1339392526091245 1102 56-57 1.2084824277155306 1102 58-59 1.1010767700329325 1102 60-61 1.139661816637222 1102 62-63 1.1938111384503713 1102 64-65 1.148440996721753 1102 66-67 0.9867603991155676 1102 68-69 0.9295878750097017 1102 70-71 1.1492021922915185 1102 72-73 1.1694704809259804 1102 74-75 1.1909463802617495 1102 76-77 1.1269767043260508 1102 78-79 0.9910189387069863 1102 80-81 1.0580977446390918 1102 82-83 1.2202721672033974 1102 84-85 1.2964953648962734 1102 86-87 1.1936452317327664 1102 88-89 1.310469317934544 1102 90-91 1.1987432336410713 1102 92-93 1.2810384954428926 1102 94-95 1.3728044332902343 1102 96-97 1.17745645818232 1102 98-99 1.2735881243681995 1102 100-101 1.57065275561893 1103 1 0.47950997001201756 1103 2 0.5571459022237626 1103 3 0.505020608523715 1103 4 0.49728615077303573 1103 5 0.38689564357864015 1103 6 0.2830102391506557 1103 7 0.37219482945308613 1103 8 0.2997685598527937 1103 9 0.5013092614662042 1103 10-11 0.494784426611524 1103 12-13 0.4613484888118222 1103 14-15 0.5651562167486688 1103 16-17 0.7050265441376311 1103 18-19 0.7162748766085159 1103 20-21 0.6338616391468648 1103 22-23 0.5989701256229623 1103 24-25 0.7722099615712423 1103 26-27 0.7002169505049523 1103 28-29 0.8176010503540638 1103 30-31 0.8649745642281133 1103 32-33 0.8585604764385479 1103 34-35 0.8002963981181637 1103 36-37 0.7974780559715242 1103 38-39 0.8758924574598481 1103 40-41 0.8581689957693328 1103 42-43 0.9098625214780114 1103 44-45 0.9093410792361354 1103 46-47 0.9163959613753221 1103 48-49 0.9294046929197322 1103 50-51 0.9422833008027638 1103 52-53 0.9858662520513306 1103 54-55 0.9942793216619776 1103 56-57 1.1851711378992107 1103 58-59 1.204259641029651 1103 60-61 1.1747607959971376 1103 62-63 1.1689491359177069 1103 64-65 1.097511813774993 1103 66-67 1.2654021793370092 1103 68-69 1.3995864138600496 1103 70-71 1.367489021380301 1103 72-73 1.2020073073089321 1103 74-75 1.2398946230940027 1103 76-77 1.2157986388881987 1103 78-79 1.306306352255632 1103 80-81 1.1725836905440872 1103 82-83 1.2459378020458658 1103 84-85 1.2583415004260203 1103 86-87 1.1660795611733121 1103 88-89 1.1599094811503718 1103 90-91 1.2153197058846388 1103 92-93 1.096193864356124 1103 94-95 0.945004978786109 1103 96-97 1.0827285026012703 1103 98-99 1.173538066463383 1103 100-101 1.5035824689088955 1104 1 0.47368401205418564 1104 2 0.5001306334304338 1104 3 0.4538020316600253 1104 4 0.37310887937603354 1104 5 0.4273720743342082 1104 6 0.3808152870359365 1104 7 0.4484999429346388 1104 8 0.39031210063853194 1104 9 0.5428988104666175 1104 10-11 0.5416983743968942 1104 12-13 0.564950228626742 1104 14-15 0.7390642911449277 1104 16-17 0.6889541964975834 1104 18-19 0.6606222441797414 1104 20-21 0.5201381425629208 1104 22-23 0.6659678852580413 1104 24-25 0.6551304588918896 1104 26-27 0.6454705557768676 1104 28-29 0.7358009877853036 1104 30-31 0.7616574113080645 1104 32-33 0.644658608458613 1104 34-35 0.7190766102464039 1104 36-37 0.7840913710051893 1104 38-39 0.7271032132297961 1104 40-41 0.81183759332108 1104 42-43 0.7337572243269008 1104 44-45 0.7730968693684375 1104 46-47 0.6869417223425103 1104 48-49 0.4121362225238414 1104 50-51 0.5023177640739007 1104 52-53 0.6475002551393985 1104 54-55 0.6111045126480406 1104 56-57 0.6273174983153922 1104 58-59 0.610407777591 1104 60-61 0.5171930047749242 1104 62-63 0.5309688955326095 1104 64-65 0.5633348955886746 1104 66-67 0.5774804710083075 1104 68-69 0.8287144375958064 1104 70-71 0.5662182197938819 1104 72-73 0.7002024007079299 1104 74-75 0.8020627311966564 1104 76-77 0.6477836324799391 1104 78-79 0.8168237049920108 1104 80-81 0.8410863799911539 1104 82-83 0.5536143317004232 1104 84-85 0.6782464362930511 1104 86-87 0.6183607976125458 1104 88-89 0.674059101196594 1104 90-91 0.7558218705615545 1104 92-93 0.8054580760403347 1104 94-95 0.7777998340201862 1104 96-97 0.6694697303616124 1104 98-99 0.6868298891314346 1104 100-101 0.8982590792973255 1105 1 0.3658013722177955 1105 2 0.42439159905676505 1105 3 0.446496532640289 1105 4 0.40516911568209935 1105 5 0.3828472883642249 1105 6 0.41594739394913205 1105 7 0.3984880400815882 1105 8 0.36695422709798464 1105 9 0.48938196005990164 1105 10-11 0.5203780036967984 1105 12-13 0.492860254862201 1105 14-15 0.5365936867652721 1105 16-17 0.6915877027337984 1105 18-19 0.6823792577637491 1105 20-21 0.7664587100043789 1105 22-23 0.5612268362527146 1105 24-25 0.67234471418152 1105 26-27 0.6369510395245541 1105 28-29 0.6028067709979581 1105 30-31 0.47837811627175597 1105 32-33 0.4541531995464254 1105 34-35 0.48079255898741025 1105 36-37 0.4929478314508202 1105 38-39 0.48463680927844166 1105 40-41 0.5938295849635153 1105 42-43 0.555546921571441 1105 44-45 0.5447727271362268 1105 46-47 0.6018680476169891 1105 48-49 0.661869146110476 1105 50-51 0.5937613096288743 1105 52-53 0.6345737321398417 1105 54-55 0.7139509422347885 1105 56-57 0.7577898595955119 1105 58-59 0.771937977883546 1105 60-61 0.6086708571067518 1105 62-63 0.6079476464806532 1105 64-65 0.7064046069592465 1105 66-67 0.8215563423512506 1105 68-69 0.9454404791692212 1105 70-71 0.9660199290829254 1105 72-73 0.9224874435434458 1105 74-75 0.9659106678223282 1105 76-77 0.7700014635440304 1105 78-79 0.8506173557544301 1105 80-81 0.886550114162084 1105 82-83 0.9589252912769588 1105 84-85 1.0862000013942819 1105 86-87 1.1417926950945514 1105 88-89 1.1481922833013911 1105 90-91 1.1013144765289162 1105 92-93 1.2093688518604253 1105 94-95 1.1733012687566458 1105 96-97 1.2435363401044413 1105 98-99 1.3736028682778318 1105 100-101 1.414041180357465 1106 1 0.5252601110159674 1106 2 0.4975096924098885 1106 3 0.5283116406726194 1106 4 0.4696352765485585 1106 5 0.4607506915199764 1106 6 0.43214729498821924 1106 7 0.4602715356008602 1106 8 0.3546162755888318 1106 9 0.5153927358034807 1106 10-11 0.5474240205201539 1106 12-13 0.6253572310566042 1106 14-15 0.7226096854457893 1106 16-17 0.7100448746508903 1106 18-19 0.8043360723219948 1106 20-21 0.8827900091412246 1106 22-23 0.8510612141734342 1106 24-25 0.8856871189316493 1106 26-27 0.9351436284519252 1106 28-29 0.8664885292034654 1106 30-31 0.8474957148203259 1106 32-33 0.8522773405107458 1106 34-35 0.7952357664204754 1106 36-37 0.71091432218698 1106 38-39 0.8385362814869168 1106 40-41 0.7785948276926291 1106 42-43 0.9017547944616453 1106 44-45 0.9663644858915248 1106 46-47 0.7367865423115845 1106 48-49 0.7085516190336705 1106 50-51 0.6398072714739484 1106 52-53 0.6122777015719194 1106 54-55 0.48778608985148253 1106 56-57 0.6522777368839812 1106 58-59 0.7329116432857106 1106 60-61 0.744330733955394 1106 62-63 0.8166973028663875 1106 64-65 0.8746000822465092 1106 66-67 1.0219066914633501 1106 68-69 0.9210288842492105 1106 70-71 0.9858576057006658 1106 72-73 0.8577659085497658 1106 74-75 0.8655827223607844 1106 76-77 1.0388654747596 1106 78-79 0.9564868373092139 1106 80-81 1.0093871310404126 1106 82-83 1.0231642543865291 1106 84-85 1.2350969995799979 1106 86-87 1.2945679988495655 1106 88-89 1.1819103271740623 1106 90-91 1.2156059438993054 1106 92-93 1.2261445129742867 1106 94-95 1.3345664565074813 1106 96-97 1.3526495403903276 1106 98-99 1.3844066507837418 1106 100-101 1.5649953284689637 1107 1 0.23495015248739648 1107 2 0.29683995578038846 1107 3 0.20335849800013506 1107 4 0.21128847846593857 1107 5 0.20427657076810846 1107 6 0.25462911757668394 1107 7 0.32598274365816593 1107 8 0.30847231048946355 1107 9 0.2829733896517084 1107 10-11 0.35790390418471674 1107 12-13 0.31793209506263764 1107 14-15 0.31924161305929744 1107 16-17 0.3640195339927388 1107 18-19 0.2635477112512774 1107 20-21 0.4335063338032583 1107 22-23 0.5386309615747393 1107 24-25 0.36121062838390117 1107 26-27 0.32939283403172226 1107 28-29 0.20335918682182097 1107 30-31 0.34331410228195125 1107 32-33 0.39701046917988947 1107 34-35 0.5791953452431144 1107 36-37 0.45797752572013906 1107 38-39 0.38675449121385697 1107 40-41 0.4437488644244638 1107 42-43 0.4525694117934762 1107 44-45 0.6262989125624969 1107 46-47 0.5376645786125493 1107 48-49 0.4863928157805901 1107 50-51 0.33996938705535484 1107 52-53 0.34202012091806466 1107 54-55 0.481922864774738 1107 56-57 0.35621375239536945 1107 58-59 0.40612487105541106 1107 60-61 0.32885960237786804 1107 62-63 0.3767047084784849 1107 64-65 0.4631484476259473 1107 66-67 0.4347618915495275 1107 68-69 0.46700117379518247 1107 70-71 0.41158699116950714 1107 72-73 0.41429954349348463 1107 74-75 0.47322504476073846 1107 76-77 0.36834236924982733 1107 78-79 0.42904675544354376 1107 80-81 0.5317468283528513 1107 82-83 0.40613794426642613 1107 84-85 0.5188605316530328 1107 86-87 0.5053457227683467 1107 88-89 0.3180716618000119 1107 90-91 0.36150767815901474 1107 92-93 0.537912838498066 1107 94-95 0.5441179457087557 1107 96-97 0.6101235534242058 1107 98-99 0.6876311889428415 1107 100-101 0.8831880898197468 1108 1 -0.06367466370275565 1108 2 0.14191084344426486 1108 3 0.049584778019017506 1108 4 0.02598281634220001 1108 5 -0.0510321155521396 1108 6 -0.057549391899321733 1108 7 -0.06270974712843724 1108 8 -0.16726659888620077 1108 9 -0.18368591821217706 1108 10-11 -0.11999548484615019 1108 12-13 -0.12053183930166966 1108 14-15 0.21169896838247837 1108 16-17 0.09312402122630203 1108 18-19 0.2917826684748448 1108 20-21 0.2578431537463999 1108 22-23 0.11392259748862443 1108 24-25 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-2.977281046681277 2316 80-81 -2.719870228676349 2316 82-83 -2.457674656326887 2316 84-85 -2.3644428665628823 2316 86-87 -2.314842253830001 2316 88-89 -2.798914276346963 2316 90-91 -2.688980723737341 2316 92-93 -3.064505251805773 2316 94-95 -3.1014841521544305 2316 96-97 -2.9528143521359844 2316 98-99 -3.159082849192007 2316 100-101 -3.0564487279838204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4801.0 3 1454.0 4 1513.0 5 1574.0 6 1803.0 7 2103.0 8 2616.0 9 3327.0 10 4088.0 11 5050.0 12 6484.0 13 7864.0 14 8643.0 15 10566.0 16 12885.0 17 16732.0 18 14720.0 19 10471.0 20 10393.0 21 11089.0 22 12085.0 23 13593.0 24 15624.0 25 18313.0 26 21478.0 27 25848.0 28 30710.0 29 37428.0 30 45754.0 31 57071.0 32 72759.0 33 94103.0 34 129043.0 35 182226.0 36 274293.0 37 406628.0 38 368645.0 39 67127.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83429413596925 19.192368510352882 14.426668020049977 22.546669333627893 2 15.10587281921505 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23.09868386020562 26.58107718729176 28.90478775895935 21.415451193543266 22-23 22.564860415488862 26.604788911272664 28.730929554810608 22.099421118427863 24-25 22.383345487242376 26.751164324582273 28.87027362840586 21.99521655976949 26-27 22.295631977219617 26.837055139082285 28.9442056284719 21.923107255226203 28-29 21.97758587056913 27.084834509060794 28.996370306379184 21.941209313990893 30-31 22.331683413391392 26.812015848585162 28.812592495480487 22.04370824254296 32-33 22.127899129101404 26.903301125832147 28.780152397925207 22.188647347141238 34-35 22.517654281555068 26.868189656287534 28.623493403462895 21.990662658694504 36-37 22.525503280405854 26.621551394517656 28.71816023625191 22.13478508882458 38-39 22.27103176202439 27.011477953860307 28.64194545571243 22.075544828402872 40-41 22.476074790120244 26.94843381096254 28.559222529892985 22.016268869024234 42-43 22.229534284393495 27.03562420102935 28.282055734998263 22.452785779578896 44-45 22.432265421632078 26.945640189780207 28.22279074025426 22.39930364833345 46-47 22.56281142047535 27.18322667430791 27.81696236687521 22.43699953834153 48-49 22.585999055718105 27.181238939638096 27.78984802434635 22.442913980297444 50-51 22.449637922303765 27.38909598466331 27.834791294217602 22.326474798815326 52-53 22.632621997860248 27.571440546992594 27.363659180849385 22.432278274297772 54-55 22.404416851560384 27.209514121949503 27.674826866593182 22.711242159896933 56-57 22.51338466869882 27.578226394882616 27.36358409845882 22.544804837959745 58-59 22.81086901463045 27.256752581089938 27.50935083765692 22.423027566622697 60-61 22.6817870951258 27.251128297663097 27.826739852286984 22.24034475492412 62-63 22.518924715398406 27.292145356742548 27.818342636559628 22.370587291299422 64-65 22.40732941914239 27.344826822796502 27.729869581970277 22.51797417609083 66-67 22.434367423003092 27.41444408090755 27.35883537708797 22.792353119001383 68-69 22.35020633914453 27.362924035151792 27.643897545922524 22.642972079781153 70-71 22.55833595721408 27.19382544900693 27.542913507439636 22.704925086339355 72-73 22.4545762702591 27.145856063125052 27.61467317222631 22.78489449438954 74-75 22.28971924262838 27.687336357931635 27.334154274411038 22.688790125028945 76-77 22.47007436974027 27.370512150473786 27.291234509828115 22.868178969957828 78-79 22.45658225741161 27.43216611387101 27.35142999954967 22.759821629167707 80-81 22.512592688405274 27.661254816314653 27.146055285944197 22.680097209335877 82-83 22.56904344053106 27.519153232327202 27.27426751979014 22.637535807351597 84-85 22.579943690342667 27.442498564392352 27.08108371707433 22.896474028190656 86-87 22.43387454544776 27.39592417639426 27.333471839843348 22.83672943831463 88-89 22.553645382389547 27.227860881744604 27.186772760790735 23.031720975075114 90-91 22.453615008802775 27.238611381204365 27.38936322461154 22.91841038538133 92-93 22.593309871859343 27.270318133307388 27.409763194715218 22.726608800118044 94-95 22.398041801349645 27.2461064601294 27.389497328156796 22.966354410364158 96-97 22.156984692159575 27.263878757742706 27.515811432014388 23.06332511808333 98-99 22.2109450402929 27.234767775938078 27.256674884321754 23.297612299447266 100-101 22.257620769537535 27.282397263308933 27.27216603760809 23.18781592954544 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 411.0 1 307.0 2 222.0 3 253.0 4 256.0 5 272.5 6 411.5 7 720.0 8 1171.0 9 1488.5 10 1446.5 11 1383.5 12 1485.5 13 1663.5 14 1840.5 15 1992.5 16 2106.5 17 2191.5 18 2367.5 19 2585.0 20 2896.5 21 3385.0 22 3995.5 23 4947.0 24 6182.0 25 7798.0 26 10115.5 27 13071.0 28 15790.5 29 19008.0 30 22886.5 31 26747.0 32 30798.5 33 34742.0 34 39812.5 35 44464.0 36 49675.5 37 55358.5 38 58017.5 39 59697.5 40 62014.5 41 64582.5 42 66197.0 43 68137.0 44 71289.5 45 73196.5 46 74720.0 47 78630.5 48 86709.5 49 86122.5 50 77044.0 51 71837.0 52 68804.5 53 66969.5 54 64617.5 55 60822.5 56 55731.0 57 49952.0 58 45621.0 59 40835.0 60 34441.5 61 27991.0 62 21741.0 63 16439.5 64 12304.0 65 9115.0 66 6422.5 67 4750.0 68 3439.5 69 2205.5 70 1440.0 71 977.0 72 674.5 73 458.5 74 311.5 75 205.0 76 146.0 77 77.5 78 42.0 79 27.5 80 24.0 81 19.5 82 12.5 83 9.5 84 10.5 85 8.5 86 7.0 87 6.5 88 5.0 89 3.5 90 2.0 91 3.0 92 3.0 93 2.0 94 3.0 95 3.5 96 2.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05718752735055655 2 0.0975171662038621 3 0.06728236913504611 4 0.12819951783455202 5 0.10214189667655928 6 0.1006997764216322 7 0.11114271619869036 8 0.09532912167914516 9 0.11825386090402043 10-11 0.10925304138188936 12-13 0.09207191903439606 14-15 0.09684583436105122 16-17 0.11181404804150123 18-19 0.08980928208270013 20-21 0.07414487241711289 22-23 0.0763826452264825 24-25 0.06397543820564436 26-27 0.056118369230524406 28-29 0.06922177223649977 30-31 0.051344453903869255 32-33 0.049355322517762944 34-35 0.04644621786558246 36-37 0.051692551896437865 38-39 0.053457906001607214 40-41 0.06884881010160483 42-43 0.06519378117963448 44-45 0.05092176348432167 46-47 0.03898697516768378 48-49 0.04649594615023512 50-51 0.029140774806457517 52-53 0.043885211205970574 54-55 0.049206137663804966 56-57 0.053333585289975574 58-59 0.06041986585297932 60-61 0.0573118480621882 62-63 0.06566619988383472 64-65 0.0443327657678445 66-67 0.06715804842341445 68-69 0.056192961657503405 70-71 0.02720137170500386 72-73 0.02200476595880111 74-75 0.031502868327458765 76-77 0.044929505183676395 78-79 0.06437326448286562 80-81 0.041423661115664014 82-83 0.06041986585297932 84-85 0.06593970544942435 86-87 0.06939582123278407 88-89 0.0910773533413429 90-91 0.06633753172664561 92-93 0.07630805279950352 94-95 0.08625370973003509 96-97 0.09246974531161732 98-99 0.12170997668738015 100-101 0.11790576291145184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2010928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.448970224919506 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42740558944203 43.33226777850781 2 13.943187353273945 16.02043510197418 3 4.638598186595936 7.994480673213465 4 2.0823042079064518 4.785049297569693 5 1.1243723479891075 3.2297016770674536 6 0.6576772345145381 2.266972791793986 7 0.43403417539292094 1.7454371493121779 8 0.2921218270833005 1.342567851692609 9 0.21365515478073918 1.1046841762859327 >10 1.0913924214714725 11.114560109877969 >50 0.06537798297355128 2.565786462966628 >100 0.028050869621034986 2.757239794260092 >500 9.547208811817562E-4 0.42731368977304085 >1k 7.81135266421436E-4 0.9956857725878466 >5k 8.679280738015955E-5 0.3178176731170782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6374 0.31696808637604135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4735 0.23546342783033503 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3555 0.17678405194019875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.141427241552159 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2238 0.11129190105264833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.486414232632894E-5 0.0 12-13 0.0 0.0 0.0 4.972828465265788E-5 0.0 14-15 0.0 0.0 0.0 1.4918485395797363E-4 0.0 16-17 0.0 0.0 0.0 1.4918485395797363E-4 0.0 18-19 0.0 0.0 0.0 2.486414232632894E-4 0.0 20-21 0.0 0.0 0.0 3.480979925686051E-4 0.0 22-23 0.0 0.0 0.0 4.475545618739209E-4 0.0 24-25 0.0 0.0 0.0 5.718752735055655E-4 0.0 26-27 0.0 0.0 0.0 8.702449814215129E-4 0.0 28-29 0.0 0.0 0.0 0.0014421202549270784 0.0 30-31 0.0 0.0 0.0 0.002933968794506815 0.0 32-33 0.0 0.0 0.0 0.004127447626170604 0.0 34-35 0.0 0.0 0.0 0.00561929616575034 0.0 36-37 0.0 0.0 0.0 0.007633291694182984 0.0 38-39 0.0 0.0 0.0 0.010194298353794866 0.0 40-41 0.0 0.0 0.0 0.01427201769531281 0.0 42-43 0.0 0.0 0.0 0.019095661306620625 0.0 44-45 0.0 0.0 0.0 0.028966725810173212 0.0 46-47 0.0 0.0 0.0 0.04992719779126851 0.0 48-49 0.0 0.0 0.0 0.06579052059546638 0.0 50-51 0.0 0.0 0.0 0.08453808390951839 0.0 52-53 0.0 0.0 0.0 0.10460344676686584 0.0 54-55 0.0 0.0 0.0 0.12710549557219353 0.0 56-57 0.0 0.0 0.0 0.1548538784083766 0.0 58-59 0.0 0.0 0.0 0.19528297383098747 0.0 60-61 0.0 0.0 0.0 0.24622960145763545 0.0 62-63 0.0 0.0 0.0 0.3095834361051216 0.0 64-65 0.0 0.0 0.0 0.358366883349379 0.0 66-67 0.0 0.0 0.0 0.4340781967330506 0.0 68-69 0.0 0.0 0.0 0.5318688685025024 0.0 70-71 0.0 0.0 0.0 0.595794578423494 0.0 72-73 0.0 0.0 0.0 0.7045006086742042 0.0 74-75 0.0 0.0 0.0 0.7954039130192627 0.0 76-77 0.0 0.0 0.0 0.8726319390848405 0.0 78-79 0.0 0.0 0.0 0.965797880381595 0.0 80-81 0.0 0.0 0.0 1.0674424942116276 0.0 82-83 0.0 0.0 0.0 1.1994710899644343 0.0 84-85 0.0 0.0 0.0 1.399229609414161 0.0 86-87 0.0 0.0 0.0 1.679125259581646 0.0 88-89 0.0 0.0 0.0 2.0022347891122902 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13790 0.0 25.713892 1 GTACATG 10225 0.0 24.266127 1 TACATGG 10170 0.0 23.50933 2 GAGTACT 5615 0.0 23.14343 12-13 ACATGGG 10270 0.0 22.305695 3 TCAACGC 15770 0.0 22.060663 4 TATCAAC 15915 0.0 21.981861 2 CAACGCA 15815 0.0 21.965105 5 ATCAACG 15920 0.0 21.852806 3 GGTATCA 10095 0.0 21.706404 1 AACGCAG 16155 0.0 21.561657 6 GTACTTT 6400 0.0 20.120148 14-15 GGACCGA 260 1.48411345E-8 20.105017 6 AGTACTT 6050 0.0 19.908693 12-13 ACGCAGA 17595 0.0 19.472435 7 CGCAGAG 17700 0.0 19.170422 8 CATGGGA 6100 0.0 18.932833 4 AGAGTAC 12630 0.0 18.90585 10-11 CATGGGG 4370 0.0 18.706226 4 GCAGAGT 18260 0.0 18.218594 9 >>END_MODULE