FastQCFastQC Report
Thu 26 May 2016
SRR936891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010928
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74920.37256430861771284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.29280014003484955No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41130.20453243477638183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36700.18250280467525443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1301.0031465E-525.5751085
GTATCAA143700.024.7950231
GTACATG97100.024.6587641
TACATGG98700.023.9179332
ACATGGG101700.022.8843143
GAGTACT72000.021.83525712-13
GGACCGT1553.9104336E-521.450096
AGTACTT75350.021.27420412-13
TCAACGC166650.021.0906144
CAACGCA168250.020.8618185
ATCAACG168350.020.8494243
TATCAAC171100.020.5431062
AACGCAG172100.020.4227226
GTACTTT77050.020.4046414-15
GGTATCA107400.020.1706831
CATGGGA62450.019.7744184
AGAGTAC136350.019.2808110-11
TAGACTG8100.018.7641035
GGACCGA2552.6792986E-718.626136
ACGCAGA190150.018.409167
Produced by FastQC (version 0.11.5)