FastQCFastQC Report
Thu 26 May 2016
SRR936890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937989
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.3188872589060103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.2377206475372151No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35520.1832827740508331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.14654365943253547No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20960.10815334865161774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134000.024.9996591
GTACATG94200.024.8682821
TACATGG93300.024.2429962
ACATGGG97900.022.2307953
TCAACGC154000.021.6312054
CAACGCA154050.021.5955685
ATCAACG154200.021.5101413
TATCAAC154550.021.4608752
GAGTACT57100.021.4298412-13
AACGCAG156550.021.2501536
GGTATCA98900.021.0920121
AGTACTT60400.020.61304712-13
CATGGGG41100.019.7714144
GTACTTT61750.019.7006614-15
ACGCAGA172350.019.137617
AGAGTAC120400.019.04579510-11
CATGGGA59400.019.0403084
CGCAGAG171500.019.0117458
GCAGAGT177000.017.803379
AGTACAT70450.016.8624232
Produced by FastQC (version 0.11.5)