##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936890_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1937989 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795592751042445 33.0 31.0 34.0 30.0 34.0 2 31.91166461729143 33.0 31.0 34.0 30.0 34.0 3 31.928660069794 33.0 31.0 34.0 30.0 34.0 4 35.5097701792941 37.0 35.0 37.0 33.0 37.0 5 35.521530823962365 37.0 35.0 37.0 33.0 37.0 6 35.589964132923356 37.0 35.0 37.0 33.0 37.0 7 35.52585489391323 37.0 35.0 37.0 33.0 37.0 8 35.47664563627554 37.0 35.0 37.0 33.0 37.0 9 37.11506670058499 39.0 37.0 39.0 33.0 39.0 10-11 36.92524286773558 39.0 37.0 39.0 33.0 39.0 12-13 36.864627972604595 39.0 37.0 39.0 33.0 39.0 14-15 38.03079119644126 40.0 38.0 41.0 33.0 41.0 16-17 37.9719580451695 40.0 38.0 41.0 33.0 41.0 18-19 37.93613973041127 40.0 38.0 41.0 33.0 41.0 20-21 37.856575037319615 40.0 38.0 41.0 32.5 41.0 22-23 37.75565315386207 40.0 37.5 41.0 32.0 41.0 24-25 37.603672415065304 40.0 37.0 41.0 32.0 41.0 26-27 37.70224314998691 40.0 37.0 41.0 32.0 41.0 28-29 37.66874012184796 40.0 37.0 41.0 32.0 41.0 30-31 37.54155415742814 40.0 37.0 41.0 32.0 41.0 32-33 37.360948127156554 40.0 37.0 41.0 31.5 41.0 34-35 37.209940046099334 40.0 36.5 41.0 31.0 41.0 36-37 37.049392695211374 39.5 36.0 41.0 31.0 41.0 38-39 36.897837139426485 39.0 36.0 41.0 30.5 41.0 40-41 36.74499287663656 39.0 36.0 41.0 30.0 41.0 42-43 36.54395896983935 39.0 35.5 41.0 30.0 41.0 44-45 36.31252138170031 39.0 35.0 41.0 30.0 41.0 46-47 35.983229780973986 39.0 35.0 40.5 28.5 41.0 48-49 35.681831011424734 39.0 35.0 40.0 27.5 41.0 50-51 35.044514700547836 38.0 34.0 39.5 27.0 40.5 52-53 35.28065664975395 38.5 35.0 40.0 28.0 40.5 54-55 35.66248157239282 39.0 35.0 40.5 28.0 41.0 56-57 35.557728913838005 39.0 35.0 41.0 28.0 41.0 58-59 35.324721141348064 38.5 35.0 40.5 27.0 41.0 60-61 35.037685456419 38.0 34.5 40.0 26.0 41.0 62-63 34.722376907196065 38.0 34.0 40.0 26.0 41.0 64-65 34.411043612734645 37.0 34.0 40.0 26.0 41.0 66-67 34.036669712779585 37.0 34.0 39.5 25.5 41.0 68-69 33.65423436355934 36.0 33.0 39.0 25.0 41.0 70-71 33.242820005686305 36.0 33.0 39.0 24.0 40.5 72-73 32.85439803837896 35.0 33.0 38.5 23.0 40.0 74-75 32.42232928050675 35.0 32.0 37.5 22.0 39.5 76-77 31.994068335785187 35.0 32.0 37.0 20.5 39.0 78-79 31.558879333164427 35.0 31.5 36.5 20.0 39.0 80-81 31.133498177750234 35.0 31.0 36.0 18.5 37.5 82-83 30.732413341871393 34.5 31.0 35.5 17.5 37.0 84-85 30.38907135179818 34.0 31.0 35.0 14.0 37.0 86-87 30.07605667524429 34.0 30.5 35.0 8.5 36.0 88-89 29.738140928560483 34.0 30.0 35.0 7.0 36.0 90-91 29.397323204620875 34.0 30.0 35.0 2.0 36.0 92-93 29.054162846125543 34.0 29.0 35.0 2.0 35.0 94-95 28.647747484634845 34.0 29.0 35.0 2.0 35.0 96-97 28.20117967645843 34.0 29.0 35.0 2.0 35.0 98-99 27.635193749809726 34.0 28.5 35.0 2.0 35.0 100-101 25.42414172629463 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45553448305462396 1101 2 0.5168269859526795 1101 3 0.47573587290025543 1101 4 0.3754568801813676 1101 5 0.4046675780424138 1101 6 0.3649373450035398 1101 7 0.3608579407746717 1101 8 0.2770342727803765 1101 9 0.3438218087863376 1101 10-11 0.39952090770405846 1101 12-13 0.4580453737973329 1101 14-15 0.6843687900912059 1101 16-17 0.828003752001969 1101 18-19 0.7017937864045294 1101 20-21 0.6868568115276972 1101 22-23 0.7390338084803361 1101 24-25 0.7544516888532584 1101 26-27 0.7112200718033321 1101 28-29 0.7946466680212794 1101 30-31 0.7778341569244489 1101 32-33 0.8109480753939096 1101 34-35 0.7414565852844603 1101 36-37 0.6923161706876897 1101 38-39 0.6691281827986657 1101 40-41 0.7135317719510113 1101 42-43 0.7633508426163829 1101 44-45 0.7708628537048483 1101 46-47 0.8596442126713342 1101 48-49 0.7714433705643344 1101 50-51 0.7955069167271276 1101 52-53 0.8536944489126626 1101 54-55 0.750452055009383 1101 56-57 0.837608823131454 1101 58-59 0.9568112775861053 1101 60-61 0.9328711320961531 1101 62-63 0.8228297284712482 1101 64-65 1.0081642474346424 1101 66-67 0.9700031822217099 1101 68-69 1.0393635596653112 1101 70-71 0.8733408608576454 1101 72-73 0.9267694499255796 1101 74-75 0.825496128080367 1101 76-77 1.0125109829054892 1101 78-79 1.0964558767965258 1101 80-81 0.819728394399263 1101 82-83 1.086613238122336 1101 84-85 1.034417936609735 1101 86-87 0.9406206151628531 1101 88-89 1.0973994037493604 1101 90-91 1.051074933159036 1101 92-93 1.2551025476671818 1101 94-95 1.2857864308937899 1101 96-97 1.4520259965497466 1101 98-99 1.5462462126579872 1101 100-101 1.5306537484811606 1102 1 0.3631672891125497 1102 2 0.33364425246272944 1102 3 0.2622629564234309 1102 4 0.32613399604341 1102 5 0.17436066997147748 1102 6 0.03944803905563532 1102 7 0.1582279907610058 1102 8 0.21852815062170095 1102 9 0.2781694638295278 1102 10-11 0.4756822730932484 1102 12-13 0.3883638671839833 1102 14-15 0.43986723093553337 1102 16-17 0.5078550821184606 1102 18-19 0.4262970625206606 1102 20-21 0.46518735364267627 1102 22-23 0.4457801573000211 1102 24-25 0.6495020641396962 1102 26-27 0.5576504610032558 1102 28-29 0.5374302943306688 1102 30-31 0.5336968267590692 1102 32-33 0.6744469407553169 1102 34-35 0.5562498943617911 1102 36-37 0.36266976452485267 1102 38-39 0.6159617243157669 1102 40-41 0.5857431003338718 1102 42-43 0.47307256166607914 1102 44-45 0.5243740100582741 1102 46-47 0.5942474114408967 1102 48-49 0.8112555995457313 1102 50-51 0.6657335999595873 1102 52-53 0.7404311794198861 1102 54-55 0.7703287894728348 1102 56-57 0.8674942146317761 1102 58-59 0.7598217371536293 1102 60-61 0.6490879903141078 1102 62-63 0.7055798045953878 1102 64-65 0.7360261538052839 1102 66-67 0.6153012387780521 1102 68-69 0.5416067212729629 1102 70-71 0.740957097413677 1102 72-73 0.5523844162642675 1102 74-75 0.6361390047503761 1102 76-77 0.6014749556297119 1102 78-79 0.5870105105894226 1102 80-81 0.7044336661902122 1102 82-83 0.6202071808636482 1102 84-85 0.544779292886254 1102 86-87 0.5203543973878091 1102 88-89 0.5246040309855751 1102 90-91 0.6329154647611119 1102 92-93 0.7077840342771111 1102 94-95 0.6550926788517728 1102 96-97 0.6446838235556989 1102 98-99 0.6506044861847897 1102 100-101 0.9850728935348734 1103 1 0.34058609052190647 1103 2 0.3788834294258301 1103 3 0.41400548680969607 1103 4 0.21711279529234417 1103 5 0.16585029305159082 1103 6 0.20205676187426747 1103 7 0.17590375556705595 1103 8 0.29320549020755493 1103 9 0.23767104125690963 1103 10-11 0.36501719073766736 1103 12-13 0.36092317029891774 1103 14-15 0.44572923489842253 1103 16-17 0.4005394515552396 1103 18-19 0.35503033379475113 1103 20-21 0.49690150728060445 1103 22-23 0.4619064266816153 1103 24-25 0.5101478191708892 1103 26-27 0.47559643768973814 1103 28-29 0.4674444641717841 1103 30-31 0.42901090372311756 1103 32-33 0.42011695819756767 1103 34-35 0.4734992930966726 1103 36-37 0.3666680054735778 1103 38-39 0.4774284934966886 1103 40-41 0.5055577380550886 1103 42-43 0.566840929243206 1103 44-45 0.7486828331671873 1103 46-47 0.7319332847023645 1103 48-49 0.8169537183881275 1103 50-51 0.7409866555301576 1103 52-53 0.6772076623551797 1103 54-55 0.5566026031193019 1103 56-57 0.6167323738656307 1103 58-59 0.5420019970187795 1103 60-61 0.66744868372556 1103 62-63 0.7520587102769838 1103 64-65 0.6375718367164396 1103 66-67 0.546934402984057 1103 68-69 0.5409850697981042 1103 70-71 0.5069157750646198 1103 72-73 0.43609971577424744 1103 74-75 0.4368284871911001 1103 76-77 0.46264625502955425 1103 78-79 0.42554289418333013 1103 80-81 0.43520668503355964 1103 82-83 0.5662856667451699 1103 84-85 0.6131516885954831 1103 86-87 0.6533099741945954 1103 88-89 0.715888678524017 1103 90-91 0.7504510138000953 1103 92-93 0.656159162287544 1103 94-95 0.6674249679800717 1103 96-97 0.9922165185048648 1103 98-99 0.950505254935738 1103 100-101 1.1968585370924139 1104 1 0.33588934998090636 1104 2 0.34953419506031835 1104 3 0.3541353326092569 1104 4 0.3708083012615475 1104 5 0.42373266035617263 1104 6 0.3149756137751183 1104 7 0.3639075697940015 1104 8 0.3407067716151957 1104 9 0.3986516259576902 1104 10-11 0.6236384066382712 1104 12-13 0.5542395637915689 1104 14-15 0.7297404776059793 1104 16-17 0.7881142178759504 1104 18-19 0.7177273732431644 1104 20-21 0.6247013961375742 1104 22-23 0.5882817691358397 1104 24-25 0.6792720520366018 1104 26-27 0.5729057973858644 1104 28-29 0.6467518094003637 1104 30-31 0.69036820122124 1104 32-33 0.7906562387358562 1104 34-35 0.8264336902877858 1104 36-37 0.7446223473668141 1104 38-39 0.8071783585147259 1104 40-41 0.8498993365992789 1104 42-43 0.899844175125061 1104 44-45 0.8194327171300628 1104 46-47 0.9749527705184775 1104 48-49 1.1597321048564595 1104 50-51 1.1466772895190829 1104 52-53 1.1624284538914722 1104 54-55 1.3446760659300594 1104 56-57 1.2172032845965077 1104 58-59 1.1666840132123966 1104 60-61 1.2581041960939032 1104 62-63 1.2459493954169645 1104 64-65 1.2897076827664904 1104 66-67 1.1707473248355527 1104 68-69 1.230364489601989 1104 70-71 1.1104183857682486 1104 72-73 1.2483404741149755 1104 74-75 1.2494232771507612 1104 76-77 1.1923326548476894 1104 78-79 1.258354455164124 1104 80-81 1.2325490403186947 1104 82-83 1.2583719615247126 1104 84-85 1.1451876690486245 1104 86-87 1.1001739197513523 1104 88-89 1.1815640566576775 1104 90-91 1.2265705220342582 1104 92-93 1.436374070399829 1104 94-95 1.4326112272906713 1104 96-97 1.396002144005454 1104 98-99 1.2307335823067085 1104 100-101 1.3168182898412546 1105 1 0.19078195090973438 1105 2 0.38242525119444437 1105 3 0.32597590199602067 1105 4 0.2674058790803855 1105 5 0.3246275713618161 1105 6 0.3420466782841345 1105 7 0.36286953732800953 1105 8 0.37225094175448703 1105 9 0.3196496866632188 1105 10-11 0.5175636899099345 1105 12-13 0.42657527387403604 1105 14-15 0.5921509739878843 1105 16-17 0.4254586414754158 1105 18-19 0.3468244589255107 1105 20-21 0.45753192014237243 1105 22-23 0.4305204670599565 1105 24-25 0.5080337206784051 1105 26-27 0.37682201796736337 1105 28-29 0.3629575806922247 1105 30-31 0.15300364416648193 1105 32-33 0.267998854540771 1105 34-35 0.40239584677058815 1105 36-37 0.31692582810919845 1105 38-39 0.28756721354041304 1105 40-41 0.272490944479685 1105 42-43 0.3343022676277556 1105 44-45 0.43952930501009035 1105 46-47 0.32857200945804976 1105 48-49 0.3961240368222505 1105 50-51 0.35823312862958545 1105 52-53 0.29469365998663477 1105 54-55 0.4509930956445629 1105 56-57 0.5799308038412221 1105 58-59 0.41819922701572665 1105 60-61 0.5513326878846456 1105 62-63 0.42488508404834135 1105 64-65 0.37912525932566865 1105 66-67 0.4951484138341229 1105 68-69 0.504212949511242 1105 70-71 0.4497381279651407 1105 72-73 0.46245173359173464 1105 74-75 0.5229136040005429 1105 76-77 0.5921646300951338 1105 78-79 0.47830033200904154 1105 80-81 0.50831742547064 1105 82-83 0.7800463903762669 1105 84-85 0.627517663232581 1105 86-87 0.5699774261875987 1105 88-89 0.6628705123552479 1105 90-91 0.76478007809947 1105 92-93 0.6726376647644621 1105 94-95 0.6221010365736142 1105 96-97 0.8619501201056714 1105 98-99 0.8456547653633031 1105 100-101 0.887777651621267 1106 1 0.26659848261027363 1106 2 0.3130778164429451 1106 3 0.20989967869263992 1106 4 0.11693247177883137 1106 5 0.17084825213346733 1106 6 0.20583704100671696 1106 7 0.2720309989228795 1106 8 0.33901341385583805 1106 9 0.2170094173375503 1106 10-11 0.3683462793684811 1106 12-13 0.42429075135797234 1106 14-15 0.444324258722709 1106 16-17 0.47315262071935393 1106 18-19 0.3718516216395358 1106 20-21 0.49534295328890465 1106 22-23 0.2426811561454585 1106 24-25 0.4255001040321602 1106 26-27 0.3718203320058038 1106 28-29 0.3717638307033724 1106 30-31 0.5311737425231655 1106 32-33 0.4907313254467809 1106 34-35 0.3688612685018242 1106 36-37 0.3774134902603379 1106 38-39 0.3232641044131981 1106 40-41 0.3963011819237323 1106 42-43 0.3263140211883453 1106 44-45 0.46648149684933315 1106 46-47 0.36060795522896427 1106 48-49 0.4540301260872255 1106 50-51 0.5947173367896355 1106 52-53 0.6039775099021583 1106 54-55 0.5557787287543547 1106 56-57 0.4086344332079932 1106 58-59 0.5422842137510457 1106 60-61 0.6730154024076498 1106 62-63 0.5509495145412302 1106 64-65 0.32996386909425723 1106 66-67 0.5146324203924308 1106 68-69 0.43939977010887077 1106 70-71 0.5083096814226096 1106 72-73 0.6028209680938161 1106 74-75 0.5469024284839179 1106 76-77 0.7144601782645736 1106 78-79 0.5364820626084423 1106 80-81 0.5812794190800474 1106 82-83 0.5071917987625447 1106 84-85 0.4812439950976142 1106 86-87 0.4938888213252497 1106 88-89 0.5298686622577478 1106 90-91 0.5338943327706573 1106 92-93 0.4647416555871189 1106 94-95 0.6533109503844052 1106 96-97 0.4683646240137129 1106 98-99 0.581411066310114 1106 100-101 0.4609457071991194 1107 1 0.2533450044501073 1107 2 0.23058320062994042 1107 3 0.2570737813692112 1107 4 0.2782954472998824 1107 5 0.29474640352152903 1107 6 0.20690500743501872 1107 7 0.27603131433520645 1107 8 0.33791686318534175 1107 9 0.3804639708566171 1107 10-11 0.5436323682063318 1107 12-13 0.40679978250000914 1107 14-15 0.40801537148755074 1107 16-17 0.4362326220992898 1107 18-19 0.43905157334202016 1107 20-21 0.5453146154625941 1107 22-23 0.4052222459936061 1107 24-25 0.41761405659217843 1107 26-27 0.45026842942878886 1107 28-29 0.5170359724023612 1107 30-31 0.6205454559513512 1107 32-33 0.48225326385267664 1107 34-35 0.5714963167254155 1107 36-37 0.4902446116004455 1107 38-39 0.24989402225814672 1107 40-41 0.5810502566320963 1107 42-43 0.5175789478303372 1107 44-45 0.49038543901058773 1107 46-47 0.38951290769534097 1107 48-49 0.4201457247053284 1107 50-51 0.33209702423572907 1107 52-53 0.3646152834853922 1107 54-55 0.41903553352890555 1107 56-57 0.4762046247717038 1107 58-59 0.46013002147766 1107 60-61 0.4865197319346706 1107 62-63 0.5099111673018228 1107 64-65 0.4762277115063682 1107 66-67 0.39632951120653814 1107 68-69 0.49960237324624046 1107 70-71 0.36991057147677253 1107 72-73 0.4187248954701346 1107 74-75 0.3860940119030829 1107 76-77 0.4827352424559095 1107 78-79 0.5061109356500566 1107 80-81 0.5329364426719003 1107 82-83 0.3567344508873873 1107 84-85 0.5707206816911139 1107 86-87 0.5624521920973713 1107 88-89 0.575269309838685 1107 90-91 0.48468890077897697 1107 92-93 0.4853372181299349 1107 94-95 0.5253670198539702 1107 96-97 0.5478695991503493 1107 98-99 0.7879594451400358 1107 100-101 0.8962212494372643 1108 1 0.21890819891136815 1108 2 0.19296185903368368 1108 3 0.23699462186401732 1108 4 0.12179202189621208 1108 5 0.1286554790256602 1108 6 0.13007187179871949 1108 7 0.13648545265857592 1108 8 0.20495068148159845 1108 9 0.15221346854477247 1108 10-11 0.1276618775142353 1108 12-13 0.2825284372725747 1108 14-15 0.32550308602917255 1108 16-17 0.38800826421117307 1108 18-19 0.37121950284890914 1108 20-21 0.33114821104709335 1108 22-23 0.33532343459658165 1108 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2316 86-87 -3.88969419109619 2316 88-89 -3.7931283939543583 2316 90-91 -3.4266839149728554 2316 92-93 -3.5319219652954885 2316 94-95 -4.005874156671613 2316 96-97 -4.305396839391523 2316 98-99 -4.186301954087707 2316 100-101 -4.254138467837574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3294.0 3 1240.0 4 1341.0 5 1417.0 6 1564.0 7 1821.0 8 2257.0 9 2828.0 10 3488.0 11 4656.0 12 5900.0 13 7041.0 14 7957.0 15 9843.0 16 12323.0 17 16393.0 18 14176.0 19 9650.0 20 9661.0 21 10101.0 22 11033.0 23 12558.0 24 14305.0 25 16738.0 26 19717.0 27 23294.0 28 28459.0 29 34083.0 30 41978.0 31 52940.0 32 67677.0 33 88475.0 34 121907.0 35 175025.0 36 265280.0 37 401019.0 38 369464.0 39 67056.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85456403797213 19.134889867386605 14.48800078463357 22.52254531000769 2 15.08619710632112 20.65266768119563 40.59430473449144 23.66683047799181 3 19.18706135800825 25.528951016612012 29.855164917518017 25.428822707861727 4 12.523146033078373 16.911019602438007 38.320383944099525 32.245450420384095 5 13.602159864683177 37.90275445737995 34.022825420231854 14.472260257705024 6 30.692160852748202 36.789378650211205 18.162516400294727 14.355944096745862 7 27.747529494012905 33.30168235532844 21.202179528684407 17.748608621974245 8 26.044537753511914 34.549908245701175 20.170610328735464 19.234943672051443 9 26.45633261165789 17.140781443870672 20.756989864702636 35.645896079768804 10-11 24.539113663737517 26.92583656392044 27.675694237355525 20.859355534986516 12-13 25.881686213507006 24.468659433152386 27.912319995869023 21.737334357471582 14-15 22.98880308979989 25.622697430416252 26.6223385716388 24.766160908145064 16-17 21.98700213172061 28.970258651176888 26.86352599702726 22.17921322007524 18-19 21.91466646291632 27.720596320826264 29.10675262040329 21.25798459585413 20-21 23.078384577357806 26.550363533340132 28.956795485552245 21.41445640374982 22-23 22.501127155822616 26.660493440043442 28.789766276788175 22.048613127345767 24-25 22.37764709919963 26.775495332393884 28.92819109200333 21.918666476403153 26-27 22.304956935201407 26.833114906466083 29.00241753256857 21.859510625763946 28-29 21.939223132016775 27.135339308182267 29.05441461378 21.87102294602096 30-31 22.29750093148694 26.827339993249943 28.867760849887652 22.00739822537546 32-33 22.12217351950683 26.913528196945663 28.83557105510586 22.12872722844165 34-35 22.55917980411615 26.86753559870801 28.650838252619877 21.92244634455596 36-37 22.454483179449735 26.651899689480658 28.83291427627836 22.06070285479125 38-39 22.281495005519034 26.9740159012178 28.76510896932098 21.979380123942185 40-41 22.41189444362452 26.932996739679005 28.676736954329705 21.97837186236677 42-43 22.14752459766746 27.036535154010526 28.383801532287485 22.432138716034526 44-45 22.453002855832942 26.941142080333297 28.278783372339056 22.327071691494705 46-47 22.534854782168736 27.179389798463156 27.899099736910475 22.38665568245763 48-49 22.58345655078679 27.158915555688566 27.826289319826756 22.431338573697886 50-51 22.387202476939947 27.400399922595625 27.883583822485967 22.328813777978457 52-53 22.65403371853642 27.580537753454713 27.39102805079015 22.374400477218714 54-55 22.349021999843124 27.255948916108313 27.66896201528306 22.726067068765506 56-57 22.497527823597053 27.562676639326494 27.447893334104062 22.49190220297239 58-59 22.785973194399432 27.29801362779724 27.512870238682552 22.40314293912078 60-61 22.65724048252938 27.271265750627784 27.91116141437147 22.160332352471368 62-63 22.454103418130327 27.28383682729137 27.931167770881952 22.33089198369635 64-65 22.39906698076938 27.33552826795404 27.821596195676012 22.443808555600565 66-67 22.386551863571935 27.413242886372135 27.436492861277127 22.763712388778803 68-69 22.310810744550473 27.377844829210247 27.68140854804258 22.6299358781967 70-71 22.532017619690286 27.258961449831105 27.57011878065963 22.638902149818975 72-73 22.404432924991433 27.180386789375678 27.67850507227528 22.73667521335761 74-75 22.287449283014134 27.672384851185015 27.37657931332756 22.66358655247329 76-77 22.467109168141956 27.30266791227735 27.397662931555068 22.832559988025622 78-79 22.39840416787002 27.428351829469573 27.425719294904006 22.7475247077564 80-81 22.494797751709974 27.666515173686573 27.209971593130632 22.62871548147282 82-83 22.510274068056088 27.528644029478656 27.348815578800888 22.61226632366437 84-85 22.510633158733278 27.488670092605638 27.16419166058947 22.83650508807161 86-87 22.346552965972112 27.43965177527872 27.397948215843794 22.815847042905375 88-89 22.48490125300762 27.248632055070814 27.241587177393633 23.02487951452793 90-91 22.43786455768979 27.252658155202614 27.45212946591234 22.85734782119526 92-93 22.495501642945552 27.341984562608975 27.391897485978443 22.77061630846703 94-95 22.316053947564683 27.268412927720153 27.42634751493024 22.989185609784926 96-97 22.14219586910194 27.28678401169476 27.540542054227103 23.030478064976204 98-99 22.162419883150534 27.289898249116582 27.269092197911494 23.27858966982139 100-101 22.19751311147627 27.287799324386246 27.344337120160887 23.170350443976595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 256.0 1 187.5 2 147.0 3 187.0 4 205.0 5 231.5 6 355.5 7 653.0 8 1119.0 9 1414.5 10 1409.5 11 1362.5 12 1454.0 13 1595.5 14 1740.0 15 1898.5 16 1991.5 17 2107.0 18 2300.5 19 2523.0 20 2810.5 21 3242.5 22 3846.5 23 4747.5 24 5884.0 25 7472.5 26 9964.0 27 12930.0 28 15327.5 29 18273.0 30 22121.5 31 26110.5 32 30073.0 33 33748.5 34 38509.0 35 43182.0 36 48211.5 37 53546.5 38 56167.5 39 57612.5 40 59738.0 41 62321.5 42 64113.0 43 66473.5 44 69222.0 45 70607.5 46 72431.5 47 76030.0 48 83782.5 49 83415.5 50 74477.5 51 69135.5 52 65861.0 53 64224.0 54 62299.5 55 58577.5 56 53201.5 57 47572.0 58 43387.0 59 38788.0 60 32867.0 61 26462.0 62 20328.0 63 15461.5 64 11696.0 65 8724.0 66 6118.0 67 4448.5 68 3224.0 69 2045.5 70 1356.5 71 927.0 72 605.0 73 425.5 74 298.5 75 195.5 76 133.0 77 65.5 78 33.5 79 21.5 80 15.5 81 16.5 82 13.5 83 8.0 84 9.5 85 6.5 86 3.0 87 5.0 88 6.0 89 5.5 90 3.0 91 0.0 92 1.0 93 2.5 94 2.5 95 2.5 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0401963065837835 2 0.04133150394558483 3 0.024509943038892375 4 0.04293110022812307 5 0.0619714559783363 6 0.066357445785296 7 0.06398385130152957 8 0.0668218447060329 9 0.06666704506578726 10-11 0.07334922953639056 12-13 0.07231723193475299 14-15 0.06677024482595104 16-17 0.0544894733664639 18-19 0.050464682720077356 20-21 0.056579268509779984 22-23 0.030469729188349365 24-25 0.016795760966651512 26-27 0.013906167682066307 28-29 0.01604756270546427 30-31 0.011713172778586464 32-33 0.007997981412691197 34-35 0.010732775057030767 36-37 0.00549538722872008 38-39 0.007739982012281804 40-41 0.007378782851708653 42-43 0.005263187768351626 44-45 0.0013157969420879065 46-47 0.002863793344544267 48-49 0.0010061976615966344 50-51 0.005882386329334171 52-53 0.004824588787655657 54-55 0.008101181172854955 56-57 0.021929949034798443 58-59 0.009545977815147558 60-61 0.021026951133365567 62-63 0.013570768461534094 64-65 0.010010376735884466 66-67 0.01818895772886224 68-69 0.0260321395013078 70-71 0.01981435395144142 72-73 0.02048515239250584 74-75 0.020562552212628656 76-77 0.02678033776249504 78-79 0.03573291695670099 80-81 0.031243727389577546 82-83 0.030289129608062793 84-85 0.02156874987422529 86-87 0.0260321395013078 88-89 0.02120755071365214 90-91 0.018937155990049477 92-93 0.03173392625035539 94-95 0.024664742679138013 96-97 0.03666171479817481 98-99 0.054308873786177325 100-101 0.0635452523208336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1937989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.72748383897643 #Duplication Level Percentage of deduplicated Percentage of total 1 75.66841894066917 43.68147431518378 2 13.82165399984174 15.957786158075763 3 4.62060031777248 8.002068905117406 4 2.0568320031165426 4.74942944877599 5 1.0788651845505588 3.114008625278836 6 0.6530735835419119 2.2620176843746886 7 0.42523020113288185 1.7183228694620196 8 0.2907268290928897 1.3426342662413322 9 0.21696729800730535 1.1272478570372795 >10 1.0731333269018346 10.999387046202196 >50 0.0654834160139955 2.585289009723416 >100 0.027045594279235977 2.6704462261593345 >500 0.0010741664069886568 0.45927942968458535 >1k 8.0562480524149E-4 1.011262200599383 >5k 8.951386724905445E-5 0.3193459580840157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6180 0.3188872589060103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4607 0.2377206475372151 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3552 0.1832827740508331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2840 0.14654365943253547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2096 0.10815334865161774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.159988008187869E-5 0.0 3 0.0 0.0 0.0 5.159988008187869E-5 0.0 4 0.0 0.0 0.0 5.159988008187869E-5 0.0 5 0.0 0.0 0.0 5.159988008187869E-5 0.0 6 0.0 0.0 0.0 5.159988008187869E-5 0.0 7 0.0 0.0 0.0 5.159988008187869E-5 0.0 8 0.0 0.0 0.0 5.159988008187869E-5 0.0 9 0.0 0.0 0.0 5.159988008187869E-5 0.0 10-11 0.0 0.0 0.0 7.739982012281804E-5 0.0 12-13 0.0 0.0 0.0 1.2899970020469672E-4 0.0 14-15 0.0 0.0 0.0 1.8059958028657542E-4 0.0 16-17 0.0 0.0 0.0 2.321994603684541E-4 0.0 18-19 0.0 0.0 0.0 2.837993404503328E-4 0.0 20-21 0.0 0.0 0.0 3.8699910061409015E-4 0.0 22-23 0.0 0.0 0.0 4.385989806959689E-4 0.0 24-25 0.0 0.0 0.0 8.513980213509984E-4 0.0 26-27 0.0 0.0 0.0 0.0011609973018422706 0.0 28-29 0.0 0.0 0.0 0.0019865953831523296 0.0 30-31 0.0 0.0 0.0 0.003482991905526812 0.0 32-33 0.0 0.0 0.0 0.004927788547819415 0.0 34-35 0.0 0.0 0.0 0.006501584890316715 0.0 36-37 0.0 0.0 0.0 0.008049581292773075 0.0 38-39 0.0 0.0 0.0 0.010397375836498555 0.0 40-41 0.0 0.0 0.0 0.013983567502189124 0.0 42-43 0.0 0.0 0.0 0.018730756469721964 0.0 44-45 0.0 0.0 0.0 0.029257132006425216 0.0 46-47 0.0 0.0 0.0 0.05072268212048675 0.0 48-49 0.0 0.0 0.0 0.06700244428631948 0.0 50-51 0.0 0.0 0.0 0.08516560207514078 0.0 52-53 0.0 0.0 0.0 0.10673435194936606 0.0 54-55 0.0 0.0 0.0 0.12915449984494237 0.0 56-57 0.0 0.0 0.0 0.15668303586862464 0.0 58-59 0.0 0.0 0.0 0.1974985410133907 0.0 60-61 0.0 0.0 0.0 0.24744722493264926 0.0 62-63 0.0 0.0 0.0 0.31527526730027877 0.0 64-65 0.0 0.0 0.0 0.36723634654273063 0.0 66-67 0.0 0.0 0.0 0.4490479564125493 0.0 68-69 0.0 0.0 0.0 0.5531765144177805 0.0 70-71 0.0 0.0 0.0 0.6204885579845912 0.0 72-73 0.0 0.0 0.0 0.7369236873893505 0.0 74-75 0.0 0.0 0.0 0.8317126670997617 0.0 76-77 0.0 0.0 0.0 0.9125180793079837 0.0 78-79 0.0 0.0 0.0 1.011073850264372 0.0 80-81 0.0 0.0 0.0 1.1142478104880884 0.0 82-83 0.0 0.0 0.0 1.2559410811929275 0.0 84-85 0.0 0.0 0.0 1.4645851963040037 0.0 86-87 0.0 0.0 0.0 1.748668336094787 0.0 88-89 0.0 0.0 0.0 2.0832935584257704 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13400 0.0 24.999659 1 GTACATG 9420 0.0 24.868282 1 TACATGG 9330 0.0 24.242996 2 ACATGGG 9790 0.0 22.230795 3 TCAACGC 15400 0.0 21.631205 4 CAACGCA 15405 0.0 21.595568 5 ATCAACG 15420 0.0 21.510141 3 TATCAAC 15455 0.0 21.460875 2 GAGTACT 5710 0.0 21.42984 12-13 AACGCAG 15655 0.0 21.250153 6 GGTATCA 9890 0.0 21.092012 1 AGTACTT 6040 0.0 20.613047 12-13 CATGGGG 4110 0.0 19.771414 4 GTACTTT 6175 0.0 19.70066 14-15 ACGCAGA 17235 0.0 19.13761 7 AGAGTAC 12040 0.0 19.045795 10-11 CATGGGA 5940 0.0 19.040308 4 CGCAGAG 17150 0.0 19.011745 8 GCAGAGT 17700 0.0 17.80337 9 AGTACAT 7045 0.0 16.862423 2 >>END_MODULE