FastQCFastQC Report
Thu 26 May 2016
SRR936890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937989
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71890.3709515379086259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.29700890975129374No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39430.20345832716284767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.1842115718923069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96450.025.7666111
TACATGG94250.025.6542782
GTATCAA141600.024.1952361
ACATGGG96050.023.391863
GAGTACT70700.022.20167412-13
GGTATCA106000.022.100341
TCAACGC163400.020.9273454
CAACGCA164750.020.7846895
ATCAACG164650.020.7428323
TATCAAC165500.020.6947692
CATGGGA59300.020.5030734
AACGCAG168450.020.326586
GTACTTT76400.020.1435414-15
TAGCCCG1200.004768462419.7889374
AGTACTT74200.019.68225712-13
CATGGGG39700.019.1409364
ACGCAGA184900.018.4668187
ATGGGAT25050.018.3906755
CGCAGAG185150.018.2879878
AGAGTAC135550.018.0429610-11
Produced by FastQC (version 0.11.5)