FastQCFastQC Report
Thu 26 May 2016
SRR936889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63710.32331107894859706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.23171219376538912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35500.18015293207777736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.14046853971585568No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20840.1057573832253769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99450.025.371231
TACATGG98300.024.6535532
GTATCAA132000.023.938641
ACATGGG101600.022.593
GGTATCA99100.022.2003151
GAGTACT60050.021.67599912-13
ATCAACG152700.020.4762153
GTACTTT62900.020.46676314-15
TCAACGC153050.020.3983424
TATCAAC154100.020.3210262
AGTACTT62000.020.15141712-13
CAACGCA155050.020.1352215
AACGCAG158350.019.7126036
CATGGGA59750.019.0869334
CATGGGG44000.018.8994064
AGAGTAC126500.017.85716610-11
AGTACAT74350.017.7675112
ACGCAGA174700.017.7580367
CGCAGAG174950.017.596888
GTGTAGC15550.017.417921
Produced by FastQC (version 0.11.5)