##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936889_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970548 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772455174905662 33.0 31.0 34.0 30.0 34.0 2 31.88399216867592 33.0 31.0 34.0 30.0 34.0 3 31.90049569967339 33.0 31.0 34.0 30.0 34.0 4 35.466089635979436 37.0 35.0 37.0 33.0 37.0 5 35.45852980998179 37.0 35.0 37.0 33.0 37.0 6 35.56719602871892 37.0 35.0 37.0 33.0 37.0 7 35.51153536985651 37.0 35.0 37.0 33.0 37.0 8 35.47047521806117 37.0 35.0 37.0 33.0 37.0 9 37.06445516678609 39.0 37.0 39.0 33.0 39.0 10-11 36.94373291084511 39.0 37.0 39.0 33.0 39.0 12-13 36.84861013281585 39.0 37.0 39.0 33.0 39.0 14-15 38.020254771769075 40.0 38.0 41.0 33.0 41.0 16-17 37.941822782292036 40.0 38.0 41.0 33.0 41.0 18-19 37.88524182105688 40.0 38.0 41.0 33.0 41.0 20-21 37.765233833431104 40.0 37.5 41.0 32.0 41.0 22-23 37.64805323189286 40.0 37.0 41.0 32.0 41.0 24-25 37.7698363602409 40.0 37.0 41.0 32.0 41.0 26-27 37.72139069943995 40.0 37.0 41.0 32.0 41.0 28-29 37.63875150465759 40.0 37.0 41.0 32.0 41.0 30-31 37.475047296488086 40.0 37.0 41.0 32.0 41.0 32-33 37.28983764922245 40.0 36.5 41.0 31.0 41.0 34-35 37.11877787295717 40.0 36.0 41.0 31.0 41.0 36-37 36.9374772905811 39.0 36.0 41.0 30.5 41.0 38-39 36.78727592527561 39.0 36.0 41.0 30.0 41.0 40-41 36.61734959006327 39.0 35.5 41.0 30.0 41.0 42-43 36.40578458378076 39.0 35.0 41.0 30.0 41.0 44-45 36.15958022844407 39.0 35.0 40.5 29.5 41.0 46-47 35.826978332930736 39.0 35.0 40.0 28.5 41.0 48-49 35.60986943733418 39.0 35.0 40.0 27.0 41.0 50-51 34.91038025970441 38.0 34.0 39.5 26.5 40.5 52-53 35.0950177311083 38.5 34.5 40.0 27.0 40.5 54-55 35.51391135866774 39.0 35.0 40.0 28.0 41.0 56-57 35.4214165298181 39.0 35.0 41.0 27.0 41.0 58-59 35.17438017241904 38.5 35.0 40.0 26.5 41.0 60-61 34.875662759800825 38.0 34.0 40.0 26.0 41.0 62-63 34.54415066265831 38.0 34.0 40.0 26.0 41.0 64-65 34.213150352084796 37.0 34.0 40.0 25.5 41.0 66-67 33.835436132487004 37.0 33.5 39.5 24.5 41.0 68-69 33.43019733596949 36.0 33.0 39.0 23.5 41.0 70-71 33.03448203240926 36.0 33.0 39.0 22.5 40.5 72-73 32.62722603052552 35.0 32.0 38.0 22.0 40.0 74-75 32.18150737764317 35.0 32.0 37.0 20.5 39.5 76-77 31.74155387232384 35.0 31.5 37.0 20.0 39.0 78-79 31.316718750317172 35.0 31.0 36.0 18.5 39.0 80-81 30.88019779269523 35.0 31.0 36.0 17.5 37.5 82-83 30.46788126957577 34.0 30.5 35.5 13.0 37.0 84-85 30.08010487438012 34.0 30.0 35.0 8.5 37.0 86-87 29.745756002898688 34.0 30.0 35.0 7.0 36.0 88-89 29.393322821874932 34.0 29.5 35.0 2.0 36.0 90-91 29.010620649687297 34.0 29.0 35.0 2.0 35.5 92-93 28.661287367777895 34.0 29.0 35.0 2.0 35.0 94-95 28.255337855256506 34.0 29.0 35.0 2.0 35.0 96-97 27.745851915304776 34.0 28.0 35.0 2.0 35.0 98-99 27.10390383791717 34.0 27.0 35.0 2.0 35.0 100-101 24.57756750913959 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4564571358683871 1101 2 0.4551336017213856 1101 3 0.41962199033288883 1101 4 0.3723384009783075 1101 5 0.4589191881169583 1101 6 0.2878093983413095 1101 7 0.2733081999026865 1101 8 0.3171565170869073 1101 9 0.39602962660977425 1101 10-11 0.4284075643226899 1101 12-13 0.6222181545846794 1101 14-15 0.6626002966484279 1101 16-17 0.7528191327742206 1101 18-19 0.7572653785739618 1101 20-21 0.7061842675655612 1101 22-23 0.7808664467879893 1101 24-25 0.6644608289227492 1101 26-27 0.7695363361746175 1101 28-29 0.6460197196027266 1101 30-31 0.7149941989391522 1101 32-33 0.7102774644497813 1101 34-35 0.8017593595089423 1101 36-37 0.7352180462443201 1101 38-39 0.825526579182764 1101 40-41 0.7813545075434405 1101 42-43 0.8802354058904172 1101 44-45 0.8923129726246231 1101 46-47 0.6487708332440789 1101 48-49 0.7070209079283458 1101 50-51 0.9478874461093909 1101 52-53 1.0141937719392686 1101 54-55 1.0297610708983527 1101 56-57 0.8961595486666027 1101 58-59 0.8719418772856145 1101 60-61 0.9395361008562872 1101 62-63 0.9955921273630821 1101 64-65 0.9091686525716014 1101 66-67 0.9332021641827382 1101 68-69 0.917724738833904 1101 70-71 0.7672022520435675 1101 72-73 0.8001018040562968 1101 74-75 0.9100729538936108 1101 76-77 0.960405786605758 1101 78-79 0.9531090477405257 1101 80-81 0.8866532609980418 1101 82-83 0.8083852027756961 1101 84-85 0.7976624983406673 1101 86-87 0.8730966194234036 1101 88-89 1.0116114112213772 1101 90-91 1.0433317604336807 1101 92-93 1.063379365424307 1101 94-95 1.0548895282824482 1101 96-97 0.9621954661203063 1101 98-99 0.9577290029429371 1101 100-101 1.5048951897415108 1102 1 0.37848981438092366 1102 2 0.524457129294106 1102 3 0.4139065609109487 1102 4 0.4637117101343975 1102 5 0.46769539290262685 1102 6 0.42720555572879704 1102 7 0.3881498033983348 1102 8 0.4422743688921713 1102 9 0.515931348318901 1102 10-11 0.6127234966607418 1102 12-13 0.5259453366974398 1102 14-15 0.6668817347285696 1102 16-17 0.7685023115668983 1102 18-19 0.6634740384901221 1102 20-21 0.6829876913674013 1102 22-23 0.6902981773367713 1102 24-25 0.3906599748726265 1102 26-27 0.616765454796365 1102 28-29 0.5257480443722713 1102 30-31 0.5544519948130855 1102 32-33 0.6221687575767945 1102 34-35 0.6151384971954101 1102 36-37 0.5538408996250226 1102 38-39 0.6229974089452952 1102 40-41 0.7297694390759872 1102 42-43 0.7068580827233006 1102 44-45 0.6694317611141969 1102 46-47 0.8703887776143446 1102 48-49 0.8660908073819087 1102 50-51 0.7931569144920019 1102 52-53 0.8167654852991575 1102 54-55 1.037334237099742 1102 56-57 0.9896529642908405 1102 58-59 0.9812493500402368 1102 60-61 0.9301053511287662 1102 62-63 1.0579118248287642 1102 64-65 1.124094943853457 1102 66-67 1.0022426328681817 1102 68-69 1.137202194044086 1102 70-71 0.9120556070040138 1102 72-73 0.8785304180455 1102 74-75 0.9629973031493506 1102 76-77 0.9981387469818053 1102 78-79 0.8625453758889847 1102 80-81 0.7588966934801427 1102 82-83 0.8821829611086898 1102 84-85 0.7916269227273176 1102 86-87 0.6360468317099084 1102 88-89 0.8625780718805096 1102 90-91 0.8531233910858163 1102 92-93 0.704090742828388 1102 94-95 0.8594736110245336 1102 96-97 0.8244625670455896 1102 98-99 0.8667294786412505 1102 100-101 0.7777322336442936 1103 1 0.3334552396772459 1103 2 0.41936982073884366 1103 3 0.3264502768590454 1103 4 0.49455366892283337 1103 5 0.5486701113769712 1103 6 0.4381587904566615 1103 7 0.4406535120356736 1103 8 0.42975470442433306 1103 9 0.4363932712936176 1103 10-11 0.4746410892252939 1103 12-13 0.5722461181182652 1103 14-15 0.6760634854296512 1103 16-17 0.7095726809281189 1103 18-19 0.6293324296535303 1103 20-21 0.6317993856568265 1103 22-23 0.6575333615688663 1103 24-25 0.4633265608070545 1103 26-27 0.6312703475065788 1103 28-29 0.5866198740900259 1103 30-31 0.6173781517229315 1103 32-33 0.6238924469953844 1103 34-35 0.5926422338647725 1103 36-37 0.7418746995234358 1103 38-39 0.7439819610551552 1103 40-41 0.779091792824488 1103 42-43 0.6828536962715148 1103 44-45 0.7351792015096947 1103 46-47 0.7513060584627524 1103 48-49 0.737339981838673 1103 50-51 0.7564732211713618 1103 52-53 0.8001363254863207 1103 54-55 0.7219470082675699 1103 56-57 0.8108733778866011 1103 58-59 0.7717411944019332 1103 60-61 0.7520855728509019 1103 62-63 0.8002062407365997 1103 64-65 0.6880260489769157 1103 66-67 0.6681601435792217 1103 68-69 0.8585506579450026 1103 70-71 0.8814744862386661 1103 72-73 0.73240253817508 1103 74-75 0.6650436131813464 1103 76-77 0.6453905710258887 1103 78-79 0.8030472557168054 1103 80-81 0.9180162971745425 1103 82-83 0.8646260813201181 1103 84-85 0.7607503443839647 1103 86-87 0.7277457632411206 1103 88-89 0.6533694384643134 1103 90-91 0.773820642937892 1103 92-93 0.7835085624523792 1103 94-95 0.8676935828355248 1103 96-97 0.7075422385550674 1103 98-99 0.872891899576917 1103 100-101 1.0552601058900066 1104 1 0.4057489435715702 1104 2 0.3939966864104889 1104 3 0.3733365352223963 1104 4 0.4009698638104666 1104 5 0.4634925509034744 1104 6 0.4227702160871303 1104 7 0.31347903957041723 1104 8 0.3973649291627126 1104 9 0.37857889489972507 1104 10-11 0.46708404685096383 1104 12-13 0.44927476797244026 1104 14-15 0.6720576525252113 1104 16-17 0.5459768668948328 1104 18-19 0.44165015138968045 1104 20-21 0.6738820756551149 1104 22-23 0.5595491447220695 1104 24-25 0.617701239139528 1104 26-27 0.5021229808913787 1104 28-29 0.44878319541444256 1104 30-31 0.5620341819167862 1104 32-33 0.520186837113755 1104 34-35 0.46629123026210095 1104 36-37 0.4556163361678003 1104 38-39 0.5419061332973953 1104 40-41 0.6374808818620536 1104 42-43 0.6071117165151279 1104 44-45 0.5384559213587252 1104 46-47 0.40794527616733234 1104 48-49 0.4411485253281242 1104 50-51 0.3134126618987594 1104 52-53 0.38427597208093545 1104 54-55 0.2830809935137495 1104 56-57 0.29020087831548835 1104 58-59 0.5121512132730928 1104 60-61 0.4691982851514638 1104 62-63 0.4673361086384773 1104 64-65 0.4463293600079723 1104 66-67 0.4159299154097198 1104 68-69 0.4534640221584141 1104 70-71 0.3541027243584196 1104 72-73 0.41786316607008445 1104 74-75 0.44361626715286206 1104 76-77 0.376167719764954 1104 78-79 0.4116422119700225 1104 80-81 0.5669869610963119 1104 82-83 0.5644716809083832 1104 84-85 0.5353600544543013 1104 86-87 0.5345136479554782 1104 88-89 0.6126935077729456 1104 90-91 0.5223109500231544 1104 92-93 0.3949599263226844 1104 94-95 0.58808301498512 1104 96-97 0.6860720035233321 1104 98-99 0.8588757733116914 1104 100-101 0.9307018626235788 1105 1 0.23919056347812173 1105 2 0.2882900597265561 1105 3 0.2695412044182781 1105 4 0.35716680906894993 1105 5 0.44055927209205237 1105 6 0.2874884816845622 1105 7 0.23436850396836206 1105 8 0.3348192102685985 1105 9 0.44355841132066587 1105 10-11 0.32051589824846616 1105 12-13 0.40493330935986904 1105 14-15 0.36243415661056844 1105 16-17 0.5009947758280973 1105 18-19 0.4876227238394506 1105 20-21 0.34628565895626195 1105 22-23 0.33001152081723717 1105 24-25 0.1512696982140227 1105 26-27 0.060165341387225624 1105 28-29 0.07567044864600092 1105 30-31 -0.09449822454406842 1105 32-33 0.17159872743383886 1105 34-35 -0.11934981867888439 1105 36-37 0.08095463705302564 1105 38-39 0.06666589612707696 1105 40-41 -0.003614629924527435 1105 42-43 0.1488701995943984 1105 44-45 -0.07756140449298243 1105 46-47 -0.04079769088477292 1105 48-49 -0.22670323565387918 1105 50-51 -0.20492184517015488 1105 52-53 -0.05126209102817825 1105 54-55 0.09718740211003762 1105 56-57 0.03406905645069003 1105 58-59 0.10288438152618795 1105 60-61 0.14378085177042266 1105 62-63 0.15342544884762788 1105 64-65 0.06962498235287029 1105 66-67 0.08897450978942345 1105 68-69 0.13601587476752286 1105 70-71 0.040383269880429395 1105 72-73 0.03047628010417469 1105 74-75 -0.009959840134136755 1105 76-77 -0.03726057256336546 1105 78-79 -0.06088480287962739 1105 80-81 0.1906945267687128 1105 82-83 0.23388185984626375 1105 84-85 0.22107077028155686 1105 86-87 0.14498526248556587 1105 88-89 0.22899404466231843 1105 90-91 0.15776495851948624 1105 92-93 0.08858502760470444 1105 94-95 0.10730615347834771 1105 96-97 0.04186117032251602 1105 98-99 0.04153250487457427 1105 100-101 0.1679603949240267 1106 1 0.16483180733292002 1106 2 0.37073865691422725 1106 3 0.353079827666658 1106 4 0.3047328401213889 1106 5 0.3966459329295162 1106 6 0.3758531438530568 1106 7 0.3599677735354234 1106 8 0.364454764498646 1106 9 0.38510243750338446 1106 10-11 0.4853695367690065 1106 12-13 0.42464704070083314 1106 14-15 0.5539572835142579 1106 16-17 0.4906508251589301 1106 18-19 0.45720818507085426 1106 20-21 0.6479634254622368 1106 22-23 0.6210140862194322 1106 24-25 0.5119234728497517 1106 26-27 0.5148049938843045 1106 28-29 0.6237595014153641 1106 30-31 0.5803835533180646 1106 32-33 0.3893774993394885 1106 34-35 0.4621333949950923 1106 36-37 0.44454083785530685 1106 38-39 0.5787840888929878 1106 40-41 0.5203846460692247 1106 42-43 0.4982817938867967 1106 44-45 0.49989759965698966 1106 46-47 0.6811242846380878 1106 48-49 0.5431631922212716 1106 50-51 0.4730570426668592 1106 52-53 0.5200541568621233 1106 54-55 0.4374645679719862 1106 56-57 0.6778002392544806 1106 58-59 0.5444189936192529 1106 60-61 0.6604132888071064 1106 62-63 0.7697618818013581 1106 64-65 0.766510123680952 1106 66-67 0.7293596740878812 1106 68-69 0.5835797719596343 1106 70-71 0.6254364318723091 1106 72-73 0.6166405557175949 1106 74-75 0.66936112032392 1106 76-77 0.6274464572009784 1106 78-79 0.6032312730968457 1106 80-81 0.6035504327041288 1106 82-83 0.7232470765673966 1106 84-85 0.890561287280768 1106 86-87 0.8321241850768963 1106 88-89 0.6906631351432679 1106 90-91 0.7967907282719402 1106 92-93 0.6041038405959505 1106 94-95 0.5254930701029004 1106 96-97 0.6181332460489521 1106 98-99 0.4609047181471375 1106 100-101 0.5557605129641594 1107 1 0.21236386510105376 1107 2 0.32462865452740886 1107 3 0.34518732473211955 1107 4 0.361542423513157 1107 5 0.3031871248709166 1107 6 0.2571504635701416 1107 7 0.22358735843000233 1107 8 0.23476460389383647 1107 9 0.4047949897033476 1107 10-11 0.35689369008817806 1107 12-13 0.34152572948548965 1107 14-15 0.3390814182931834 1107 16-17 0.45328340518359767 1107 18-19 0.522419650105661 1107 20-21 0.5429933036772354 1107 22-23 0.587487314808385 1107 24-25 0.5176446654171301 1107 26-27 0.410553306328417 1107 28-29 0.3667742397101108 1107 30-31 0.4699016528671933 1107 32-33 0.35014136193051826 1107 34-35 0.41196604306433926 1107 36-37 0.5428645470688664 1107 38-39 0.4698439430415249 1107 40-41 0.3143501569256557 1107 42-43 0.2670844266006256 1107 44-45 0.14725235445159512 1107 46-47 0.35877190113039603 1107 48-49 0.14379415762054748 1107 50-51 -0.08257000324005759 1107 52-53 0.1405584178914765 1107 54-55 0.23354679583964355 1107 56-57 0.26606395789585946 1107 58-59 0.2753531898700672 1107 60-61 0.20931842323459193 1107 62-63 0.12215160143043136 1107 64-65 0.39473832916508655 1107 66-67 0.2577030631908528 1107 68-69 0.19301890347371398 1107 70-71 0.2269042166554982 1107 72-73 0.32201466833663517 1107 74-75 0.2637317688332601 1107 76-77 0.18859448021667546 1107 78-79 0.22707134347329827 1107 80-81 0.3632022871699512 1107 82-83 0.2174715295646692 1107 84-85 0.321785724270363 1107 86-87 0.2639690988516499 1107 88-89 0.2601705119360993 1107 90-91 0.23306296013195293 1107 92-93 0.2287236283341123 1107 94-95 0.2689727478754733 1107 96-97 0.12312226645300939 1107 98-99 0.1205293595571888 1107 100-101 0.17133464751611172 1108 1 0.04658673932562252 1108 2 0.1785913656858682 1108 3 0.045103653827844425 1108 4 0.18091555520084057 1108 5 0.26361645428731606 1108 6 0.15579117997000935 1108 7 0.036892506586475804 1108 8 0.16675319774346775 1108 9 0.18122596489326526 1108 10-11 0.17126637946271472 1108 12-13 0.2600638568790501 1108 14-15 0.33043062544685853 1108 16-17 0.3369858012307887 1108 18-19 0.4801468378049307 1108 20-21 0.3646429658605683 1108 22-23 0.36784093956465114 1108 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-2.0277260933497665 2316 82-83 -1.6142356376297116 2316 84-85 -1.4959394340188261 2316 86-87 -1.7192297077008476 2316 88-89 -1.8144459458643034 2316 90-91 -1.5865659431768435 2316 92-93 -2.2598990784522357 2316 94-95 -1.8221340085491207 2316 96-97 -1.9607547280101478 2316 98-99 -1.8593842845479038 2316 100-101 -2.071052194054797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3254.0 3 1237.0 4 1315.0 5 1432.0 6 1611.0 7 1933.0 8 2483.0 9 3052.0 10 4039.0 11 4974.0 12 6226.0 13 7508.0 14 8221.0 15 10500.0 16 13049.0 17 16942.0 18 14961.0 19 10347.0 20 10113.0 21 11052.0 22 12003.0 23 13610.0 24 15658.0 25 17934.0 26 21097.0 27 25430.0 28 30463.0 29 37080.0 30 44871.0 31 56308.0 32 71948.0 33 93205.0 34 127807.0 35 181690.0 36 273904.0 37 401183.0 38 352747.0 39 59339.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84529483723929 19.15774566385966 14.485881212342708 22.511078286558345 2 15.098375873131234 20.703976093599564 40.53912221731052 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28.948415548268464 21.439494331582505 22-23 22.557023074149345 26.593013580686442 28.81892257366937 22.031040771494844 24-25 22.359662007912124 26.758451261202946 28.91184279069555 21.970043940189377 26-27 22.30912982305679 26.836298033922045 28.993236651235254 21.861335491785912 28-29 22.02345745643182 27.108858218216813 28.985865795214266 21.881818530137096 30-31 22.31668551083252 26.751264115362833 28.903152828553274 22.028897545251372 32-33 22.11884959511119 26.876197957819702 28.85839472510341 22.146557721965692 34-35 22.512302421611867 26.866550252290356 28.6954586721352 21.92568865396258 36-37 22.51081932538563 26.609653761288737 28.81056437092626 22.068962542399372 38-39 22.30840530786764 26.95939933414411 28.749841731722647 21.982353626265606 40-41 22.45767292026021 26.92567089239688 28.62187504281806 21.994781144524843 42-43 22.2115906483763 27.04936272722452 28.342760925845152 22.39628569855403 44-45 22.466136880510376 26.93607119051165 28.255199644159084 22.34259228481888 46-47 22.559257627827385 27.194211965402516 27.871307879838504 22.375222526931594 48-49 22.541381370866144 27.170055961746577 27.862377999967013 22.426184667420266 50-51 22.443353883480487 27.353390216059463 27.895803471678278 22.307452428781776 52-53 22.635571975194615 27.582896058948307 27.38244339115166 22.399088574705413 54-55 22.386584968530926 27.25667924888328 27.682788075753134 22.67394770683266 56-57 22.51385978306956 27.54395433331836 27.42482452140645 22.51736136220563 58-59 22.770692232819528 27.277137733047386 27.537449467704494 22.414720566428592 60-61 22.69478999486168 27.217097291913806 27.888580871484848 22.19953184173967 62-63 22.456973516640307 27.28990564077216 27.922155857277957 22.330964985309578 64-65 22.426711081271318 27.331551065377056 27.778031517520986 22.46370633583064 66-67 22.459706725459625 27.42130148444098 27.3425065370623 22.776485253037094 68-69 22.31409195268702 27.391768978574454 27.698837934334446 22.59530113440408 70-71 22.615846000936045 27.18613228723453 27.58712498464456 22.610896727184862 72-73 22.472523749474355 27.143061255409663 27.639761719803786 22.744653275312196 74-75 22.310360011115918 27.687368585347038 27.330060312743083 22.672211090793965 76-77 22.451739485229634 27.346842427880496 27.374787902477653 22.82663018441221 78-79 22.436259084680888 27.442112719200402 27.426530097934492 22.695098098184218 80-81 22.50534234953715 27.677889196954364 27.15703232927455 22.659736124233934 82-83 22.564721906416867 27.554184203065084 27.291699823055932 22.589394067462116 84-85 22.513619569746968 27.490821732229588 27.133005066129716 22.862553631893725 86-87 22.381035402041224 27.406135142453493 27.33890981274104 22.873919642764243 88-89 22.540826352994678 27.245119515037334 27.21306849326193 23.000985638706062 90-91 22.457322881169024 27.257875353194542 27.395054299259236 22.8897474663772 92-93 22.548628927701557 27.25379939672616 27.431471766845117 22.766099908727167 94-95 22.43252440698345 27.187594291490097 27.416368127173385 22.96351317435307 96-97 22.149564421177708 27.27966705825237 27.558903448365125 23.011865072204802 98-99 22.24635076883093 27.204386387032713 27.263853271363914 23.28540957277244 100-101 22.336030671422186 27.195325979048945 27.27046121612981 23.198182133399058 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 197.0 2 142.0 3 184.5 4 199.0 5 224.0 6 345.0 7 611.5 8 1053.5 9 1361.5 10 1387.5 11 1385.5 12 1481.5 13 1651.5 14 1804.0 15 1946.0 16 2076.5 17 2179.0 18 2349.0 19 2561.5 20 2871.0 21 3320.0 22 3913.0 23 4791.0 24 5881.0 25 7516.0 26 10108.0 27 13042.0 28 15618.0 29 18714.5 30 22363.0 31 26196.0 32 30131.0 33 34078.5 34 39326.5 35 44021.5 36 48962.0 37 54401.5 38 56918.5 39 58501.0 40 60594.0 41 62900.5 42 64706.5 43 67149.5 44 70272.0 45 71945.0 46 73706.5 47 77771.0 48 85348.0 49 84660.0 50 75734.0 51 70293.5 52 67225.0 53 65351.5 54 63282.5 55 59634.0 56 54338.5 57 48634.5 58 44337.0 59 39693.0 60 33581.0 61 27020.5 62 20944.0 63 15936.0 64 11924.5 65 8888.0 66 6272.0 67 4639.5 68 3319.5 69 2109.5 70 1407.5 71 956.5 72 654.5 73 458.5 74 324.0 75 204.5 76 139.0 77 71.0 78 34.5 79 24.5 80 18.0 81 12.0 82 12.0 83 11.0 84 10.0 85 9.5 86 6.0 87 5.5 88 6.0 89 4.5 90 4.0 91 2.5 92 2.0 93 2.0 94 2.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024257211699486643 2 0.009997219047696376 3 0.01111365975353049 4 0.01862426086550543 5 0.053081680831931016 6 0.039582897752300374 7 0.01476746569989668 8 0.04546958511033479 9 0.026540840415965508 10-11 0.02184671472098117 12-13 0.03311261638894358 14-15 0.01656899502067445 16-17 0.01519881779078713 18-19 0.01545255431484034 20-21 0.01243308967860717 22-23 0.007079249021084491 24-25 0.001446298187103283 26-27 2.5373652405320752E-5 28-29 0.002258255064073547 30-31 0.0 32-33 3.5523113367449055E-4 34-35 5.0747304810641504E-5 36-37 0.0 38-39 7.612095721596227E-5 40-41 5.0747304810641504E-5 42-43 1.2686826202660376E-4 44-45 2.283628716478868E-4 46-47 0.0 48-49 2.791101764585283E-4 50-51 1.2686826202660376E-4 52-53 4.0597843848513203E-4 54-55 0.0021313868020469435 56-57 4.313520908904528E-4 58-59 0.001319429925076679 60-61 0.0030702119410438112 62-63 0.0023597496736948304 64-65 0.0012686826202660377 66-67 0.012382342373796528 68-69 0.015554048924461623 70-71 0.028519985303580526 72-73 0.017583941116887283 74-75 0.020324295576661926 76-77 0.03250364873121588 78-79 0.020527284795904488 80-81 0.04612930007287313 82-83 0.03636044389682464 84-85 0.02055265844830981 86-87 0.015655543534082904 88-89 0.012788320812281661 90-91 0.025043794924051583 92-93 0.031970802030704154 94-95 0.013270420207982754 96-97 0.017939172250561772 98-99 0.02760653381698898 100-101 0.038897809137356715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1970548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.66469940969453 #Duplication Level Percentage of deduplicated Percentage of total 1 75.60175617548836 43.59552544704553 2 13.739729186827365 15.845947070580133 3 4.703100944518027 8.136087067772474 4 2.0950552468802526 4.8324292423221245 5 1.109680830457989 3.199470576453006 6 0.6514030380631967 2.2537776230685624 7 0.42298614303330734 1.7073958154737205 8 0.29824239326795593 1.3758446367219663 9 0.21072395495188687 1.093620016865031 >10 1.0736539021532359 10.960686602774475 >50 0.06483322895548951 2.5686420632672613 >100 0.027066762770990942 2.71821902805799 >500 8.840963160597885E-4 0.3964294092959314 >1k 7.956866844538097E-4 0.9911245043881434 >5k 8.840963160597886E-5 0.324800895913629 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6371 0.32331107894859706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4566 0.23171219376538912 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3550 0.18015293207777736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2768 0.14046853971585568 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2084 0.1057573832253769 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.0747304810641504E-5 0.0 4 0.0 0.0 0.0 5.0747304810641504E-5 0.0 5 0.0 0.0 0.0 5.0747304810641504E-5 0.0 6 0.0 0.0 0.0 5.0747304810641504E-5 0.0 7 0.0 0.0 0.0 5.0747304810641504E-5 0.0 8 0.0 0.0 0.0 5.0747304810641504E-5 0.0 9 0.0 0.0 0.0 5.0747304810641504E-5 0.0 10-11 0.0 0.0 0.0 1.0149460962128301E-4 0.0 12-13 0.0 0.0 0.0 1.0149460962128301E-4 0.0 14-15 0.0 0.0 0.0 1.0149460962128301E-4 0.0 16-17 0.0 0.0 0.0 1.0149460962128301E-4 0.0 18-19 0.0 0.0 0.0 1.0149460962128301E-4 0.0 20-21 0.0 0.0 0.0 1.2686826202660376E-4 0.0 22-23 0.0 0.0 0.0 3.5523113367449055E-4 0.0 24-25 0.0 0.0 0.0 5.582203529170566E-4 0.0 26-27 0.0 0.0 0.0 7.865832245649433E-4 0.0 28-29 0.0 0.0 0.0 0.0015224191443192453 0.0 30-31 0.0 0.0 0.0 0.00304483828863849 0.0 32-33 0.0 0.0 0.0 0.0045672574329577355 0.0 34-35 0.0 0.0 0.0 0.00603892927246634 0.0 36-37 0.0 0.0 0.0 0.007865832245649433 0.0 38-39 0.0 0.0 0.0 0.00979422982845381 0.0 40-41 0.0 0.0 0.0 0.012864441769497623 0.0 42-43 0.0 0.0 0.0 0.01745707285486068 0.0 44-45 0.0 0.0 0.0 0.02692144520204532 0.0 46-47 0.0 0.0 0.0 0.047093498864275316 0.0 48-49 0.0 0.0 0.0 0.06302815257481675 0.0 50-51 0.0 0.0 0.0 0.08053597273448806 0.0 52-53 0.0 0.0 0.0 0.1013423677068511 0.0 54-55 0.0 0.0 0.0 0.12316370877542693 0.0 56-57 0.0 0.0 0.0 0.15005978032506695 0.0 58-59 0.0 0.0 0.0 0.19289050558524837 0.0 60-61 0.0 0.0 0.0 0.24394229422475372 0.0 62-63 0.0 0.0 0.0 0.31095411022720587 0.0 64-65 0.0 0.0 0.0 0.36319846052976124 0.0 66-67 0.0 0.0 0.0 0.4429224763872791 0.0 68-69 0.0 0.0 0.0 0.548045518302523 0.0 70-71 0.0 0.0 0.0 0.6156155546578921 0.0 72-73 0.0 0.0 0.0 0.7270312623696555 0.0 74-75 0.0 0.0 0.0 0.8216242385366913 0.0 76-77 0.0 0.0 0.0 0.9003079346455909 0.0 78-79 0.0 0.0 0.0 1.0009144664326877 0.0 80-81 0.0 0.0 0.0 1.104641957465639 0.0 82-83 0.0 0.0 0.0 1.2410507127966435 0.0 84-85 0.0 0.0 0.0 1.4431772278574284 0.0 86-87 0.0 0.0 0.0 1.7186589720219958 0.0 88-89 0.0 0.0 0.0 2.043061118024022 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9945 0.0 25.37123 1 TACATGG 9830 0.0 24.653553 2 GTATCAA 13200 0.0 23.93864 1 ACATGGG 10160 0.0 22.59 3 GGTATCA 9910 0.0 22.200315 1 GAGTACT 6005 0.0 21.675999 12-13 ATCAACG 15270 0.0 20.476215 3 GTACTTT 6290 0.0 20.466763 14-15 TCAACGC 15305 0.0 20.398342 4 TATCAAC 15410 0.0 20.321026 2 AGTACTT 6200 0.0 20.151417 12-13 CAACGCA 15505 0.0 20.135221 5 AACGCAG 15835 0.0 19.712603 6 CATGGGA 5975 0.0 19.086933 4 CATGGGG 4400 0.0 18.899406 4 AGAGTAC 12650 0.0 17.857166 10-11 AGTACAT 7435 0.0 17.767511 2 ACGCAGA 17470 0.0 17.758036 7 CGCAGAG 17495 0.0 17.59688 8 GTGTAGC 1555 0.0 17.41792 1 >>END_MODULE