FastQCFastQC Report
Thu 26 May 2016
SRR936889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72420.3675119814386658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56330.2858595679983436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40020.2030907138521873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.18167535122209658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146250.024.5253451
GTACATG94850.023.9917661
TACATGG95150.023.8108252
ACATGGG96100.022.4369723
GAGTACT71800.021.16690412-13
TCAACGC169200.021.0519664
ATCAACG169650.020.9681323
TATCAAC170000.020.9544932
CAACGCA170800.020.8595245
AGTACTT75550.020.80775812-13
AACGCAG174750.020.4423896
GGTATCA108550.020.394831
GTACTTT76200.019.91350414-15
GTCTACG1250.00602699219.003081
AGAGTAC137750.018.63953410-11
CATGGGA59100.018.4829854
ACGCAGA192650.018.4443597
CGCAGAG192400.018.2708028
CATGGGG42150.017.8029214
TACTTTT89450.017.44161814-15
Produced by FastQC (version 0.11.5)