##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936889_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970548 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22655119286615 33.0 31.0 34.0 31.0 34.0 2 32.34572920832175 34.0 31.0 34.0 31.0 34.0 3 32.459541711239716 34.0 31.0 34.0 31.0 34.0 4 36.09602760247403 37.0 35.0 37.0 35.0 37.0 5 36.00500317678128 37.0 35.0 37.0 35.0 37.0 6 36.04612625523458 37.0 35.0 37.0 35.0 37.0 7 35.9949080154353 37.0 35.0 37.0 35.0 37.0 8 35.95946153049812 37.0 35.0 37.0 35.0 37.0 9 37.65347405899273 39.0 37.0 39.0 35.0 39.0 10-11 37.569084082194394 39.0 37.0 39.0 35.0 39.0 12-13 37.48096189486377 39.0 37.0 39.0 35.0 39.0 14-15 38.701175003095585 40.0 38.0 41.0 34.5 41.0 16-17 38.61656199189261 40.0 38.0 41.0 34.0 41.0 18-19 38.77373172335817 40.0 38.0 41.0 35.0 41.0 20-21 38.80002161835185 40.0 38.0 41.0 35.0 41.0 22-23 38.72791984767689 40.0 38.0 41.0 34.0 41.0 24-25 38.62560922139425 40.0 38.0 41.0 34.0 41.0 26-27 38.45487016809537 40.0 38.0 41.0 34.0 41.0 28-29 38.38510353465127 40.0 38.0 41.0 34.0 41.0 30-31 38.39630118119427 40.0 38.0 41.0 34.0 41.0 32-33 38.25170206460335 40.0 38.0 41.0 34.0 41.0 34-35 38.11112670181086 40.0 38.0 41.0 33.5 41.0 36-37 37.9643180475685 40.0 37.0 41.0 33.0 41.0 38-39 37.82212815927346 40.0 37.0 41.0 33.0 41.0 40-41 37.658077600748626 40.0 37.0 41.0 33.0 41.0 42-43 37.42342561561556 40.0 37.0 41.0 32.5 41.0 44-45 37.170406912188895 40.0 37.0 41.0 32.0 41.0 46-47 36.84618085933456 40.0 36.0 41.0 31.0 41.0 48-49 36.51592602666872 39.0 36.0 41.0 30.5 41.0 50-51 36.24890969415614 39.0 35.5 40.5 30.0 41.0 52-53 36.01880162269582 39.0 35.0 40.0 29.5 41.0 54-55 35.749932252348074 39.0 35.0 40.0 28.5 41.0 56-57 35.46374282686847 38.0 35.0 40.0 28.0 41.0 58-59 35.11844750800285 38.0 34.0 40.0 27.5 41.0 60-61 34.785558636480815 38.0 34.0 40.0 27.0 41.0 62-63 34.42496046784955 37.0 34.0 40.0 26.0 41.0 64-65 34.038541563057585 37.0 33.0 39.0 26.0 40.5 66-67 33.64534865428297 36.0 33.0 39.0 26.0 40.0 68-69 33.333205027231 36.0 33.0 39.0 25.0 40.0 70-71 32.8686822142876 35.0 32.0 38.0 24.5 40.0 72-73 32.371287073443526 35.0 31.5 37.5 22.5 39.5 74-75 31.860712603803613 35.0 31.0 37.0 21.5 39.0 76-77 30.421721013646966 33.5 29.5 35.5 20.5 37.5 78-79 31.184591545093042 34.0 31.0 36.0 21.0 38.0 80-81 30.972711905520697 34.0 31.0 35.5 20.0 37.0 82-83 30.648223489100495 34.0 31.0 35.0 19.0 37.0 84-85 30.295432793314347 34.0 30.0 35.0 18.0 36.5 86-87 29.956022385651096 34.0 30.0 35.0 16.0 36.0 88-89 29.57965271589426 34.0 30.0 35.0 9.0 36.0 90-91 29.122909718514848 34.0 29.0 35.0 4.5 35.0 92-93 28.65511243572854 34.0 29.0 35.0 2.0 35.0 94-95 28.09749293090044 33.0 28.5 35.0 2.0 35.0 96-97 27.465249006875247 33.0 27.0 34.5 2.0 35.0 98-99 26.664910471604852 33.0 25.5 34.0 2.0 35.0 100-101 23.10499617365322 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2817439774701711 1101 2 0.23746002982301917 1101 3 0.2755416869874452 1101 4 0.2592811083014581 1101 5 0.27874554982297184 1101 6 0.2529419756409865 1101 7 0.294332286346048 1101 8 0.28915817304937974 1101 9 0.3727887167283228 1101 10-11 0.40743880477658223 1101 12-13 0.3571056473024683 1101 14-15 0.4982265565378654 1101 16-17 0.48081691309462116 1101 18-19 0.39631142106296835 1101 20-21 0.454311587524586 1101 22-23 0.48807676496288366 1101 24-25 0.4747918276280103 1101 26-27 0.36906891614451354 1101 28-29 0.42508801286039244 1101 30-31 0.5183107730331784 1101 32-33 0.5578181622902676 1101 34-35 0.4968031678954432 1101 36-37 0.5129618997789223 1101 38-39 0.5322424112813522 1101 40-41 0.6148920539874894 1101 42-43 0.7035965559341193 1101 44-45 0.6870416848937069 1101 46-47 0.8025936364000472 1101 48-49 0.8891585062466589 1101 50-51 0.685881914948034 1101 52-53 0.5973624003710398 1101 54-55 0.6941119908387563 1101 56-57 0.8269868369173778 1101 58-59 0.8499166438998387 1101 60-61 0.919765377941772 1101 62-63 0.8858091319536499 1101 64-65 0.736211332887386 1101 66-67 0.5190567741539951 1101 68-69 0.6383175366825142 1101 70-71 0.6836366568389494 1101 72-73 0.7023066894864627 1101 74-75 0.7455384490765908 1101 76-77 0.6705719488387132 1101 78-79 0.7437452681733419 1101 80-81 0.9023169325898515 1101 82-83 0.8830994240250796 1101 84-85 0.7883526523844786 1101 86-87 0.34377795733906424 1101 88-89 0.4012986001593646 1101 90-91 0.5018795177151034 1101 92-93 0.6132558926835081 1101 94-95 0.40508076534319315 1101 96-97 0.3585943566021079 1101 98-99 0.7285403679275291 1101 100-101 0.8712428023168357 1102 1 0.31338067391702396 1102 2 0.3516588888826817 1102 3 0.2923557220493578 1102 4 0.22076364416580674 1102 5 0.22820076491095165 1102 6 0.21312982743398834 1102 7 0.2369622114480876 1102 8 0.2499746699726657 1102 9 0.4054004108150764 1102 10-11 0.3226038953860808 1102 12-13 0.392288319133165 1102 14-15 0.5094620173951299 1102 16-17 0.46221977562148453 1102 18-19 0.3420294006201985 1102 20-21 0.34984094289206524 1102 22-23 0.3604706922080112 1102 24-25 0.42482128291666044 1102 26-27 0.2590698111439167 1102 28-29 0.3459958119832933 1102 30-31 0.47350103396841803 1102 32-33 0.47717549754600697 1102 34-35 0.45116494136741636 1102 36-37 0.35522469122076217 1102 38-39 0.47026937283192893 1102 40-41 0.502754145613352 1102 42-43 0.447719750763234 1102 44-45 0.33800126524932494 1102 46-47 0.30579739711398446 1102 48-49 0.5972688559952672 1102 50-51 0.5507497247618858 1102 52-53 0.5269138401553519 1102 54-55 0.5606600700842037 1102 56-57 0.5646668022943899 1102 58-59 0.5750376527235517 1102 60-61 0.6378470018875362 1102 62-63 0.6566273698346876 1102 64-65 0.5920287677210112 1102 66-67 0.5856546913800429 1102 68-69 0.5330769473560579 1102 70-71 0.46576115861239487 1102 72-73 0.7141090343849044 1102 74-75 0.7911221174216934 1102 76-77 0.557064722025018 1102 78-79 0.5067250583511509 1102 80-81 0.6872283460391166 1102 82-83 0.5668527634149889 1102 84-85 0.46922528906498684 1102 86-87 0.47139307203998015 1102 88-89 0.6032161363853845 1102 90-91 0.8253483944172864 1102 92-93 0.8043799506353331 1102 94-95 0.5452445686247778 1102 96-97 0.7512690623250791 1102 98-99 1.0304932443697936 1102 100-101 1.2482985423893922 1103 1 0.33724181670432785 1103 2 0.26957942817405467 1103 3 0.24911491683494802 1103 4 0.23442701538722588 1103 5 0.24927131928355095 1103 6 0.2123286720659081 1103 7 0.22497403423633244 1103 8 0.1671627323652345 1103 9 0.3187595784656665 1103 10-11 0.323498677728594 1103 12-13 0.3594202053410669 1103 14-15 0.48922247250072104 1103 16-17 0.3367712631691049 1103 18-19 0.3676526367048254 1103 20-21 0.3214430966488706 1103 22-23 0.29890338786876924 1103 24-25 0.3534538413326018 1103 26-27 0.48984068070864595 1103 28-29 0.4290317254966354 1103 30-31 0.5256257495834973 1103 32-33 0.5345451458113288 1103 34-35 0.5576872094500445 1103 36-37 0.45219168182555336 1103 38-39 0.41264407246875123 1103 40-41 0.4745931700090722 1103 42-43 0.45161325933037944 1103 44-45 0.3461688171674453 1103 46-47 0.29083852570587254 1103 48-49 0.3449192113853172 1103 50-51 0.3885472987580272 1103 52-53 0.34211731904219533 1103 54-55 0.35088561017729347 1103 56-57 0.33709831163970705 1103 58-59 0.29121378692744315 1103 60-61 0.39323419708128426 1103 62-63 0.45121034856286 1103 64-65 0.27731598948911795 1103 66-67 0.3184535380302691 1103 68-69 0.25365284855773496 1103 70-71 0.3622768615939975 1103 72-73 0.3427637100711536 1103 74-75 0.5624458010096163 1103 76-77 0.4192196057089177 1103 78-79 0.4612398687929691 1103 80-81 0.497322567292553 1103 82-83 0.5253144834378141 1103 84-85 0.4885466448732778 1103 86-87 0.3242774836533151 1103 88-89 0.29659522359526846 1103 90-91 0.4525808651490486 1103 92-93 0.4525488424844042 1103 94-95 0.5278732608633803 1103 96-97 0.5248480627478038 1103 98-99 0.34392187538644947 1103 100-101 0.6138567946260665 1104 1 0.25729671462849524 1104 2 0.23617747243550724 1104 3 0.22164879914752333 1104 4 0.10165981689691961 1104 5 0.18504074800203085 1104 6 0.10146917644058817 1104 7 0.09062852042607261 1104 8 0.1727383027889715 1104 9 0.2636386314764749 1104 10-11 0.24792825398524343 1104 12-13 0.14560994629149349 1104 14-15 0.13832180343226952 1104 16-17 0.23689135237822967 1104 18-19 0.2571791421088392 1104 20-21 0.2498441946073484 1104 22-23 0.1589651480334595 1104 24-25 0.2809028034187264 1104 26-27 0.095750785357815 1104 28-29 0.10446490442956247 1104 30-31 0.24375922016163543 1104 32-33 0.1415017421400293 1104 34-35 0.21080952888733862 1104 36-37 0.22266689198166745 1104 38-39 0.2625668722628802 1104 40-41 0.2356842662519938 1104 42-43 0.10344033086924043 1104 44-45 0.041596849419974546 1104 46-47 0.0640071652666876 1104 48-49 0.11653526902813383 1104 50-51 0.1579417060320054 1104 52-53 0.22642852164984362 1104 54-55 0.046202131021352955 1104 56-57 0.18984320784831965 1104 58-59 0.28829733470579555 1104 60-61 0.38230607905074976 1104 62-63 0.1899476963068949 1104 64-65 0.03121031098508098 1104 66-67 0.015631441615788333 1104 68-69 0.15749570324978635 1104 70-71 -0.04044230121266423 1104 72-73 0.06545199279546665 1104 74-75 0.10379080015183106 1104 76-77 0.07486379853787639 1104 78-79 0.08507757680463612 1104 80-81 0.37093725248726983 1104 82-83 0.28088147606090885 1104 84-85 0.3940879040944161 1104 86-87 0.1540167185134962 1104 88-89 0.0349894593132305 1104 90-91 0.23961123056335865 1104 92-93 0.13853271621917784 1104 94-95 0.22087377553141962 1104 96-97 0.2527087592652144 1104 98-99 0.009705028585969444 1104 100-101 0.5667560912464147 1105 1 0.18972740743539163 1105 2 0.19084174619607808 1105 3 0.1137885569793724 1105 4 0.07910667696491913 1105 5 0.13554848329815172 1105 6 0.14208497113530427 1105 7 0.168513827528308 1105 8 0.14551142348685175 1105 9 0.17898503492347118 1105 10-11 0.19192970790286523 1105 12-13 0.21583265315292266 1105 14-15 0.2735225122171059 1105 16-17 0.1641962283030196 1105 18-19 0.09302919170863078 1105 20-21 0.18506194148937283 1105 22-23 0.17864301440934582 1105 24-25 0.2034832043062167 1105 26-27 0.24814672044644936 1105 28-29 0.23166149378112522 1105 30-31 0.1216566432440942 1105 32-33 0.12173111279059157 1105 34-35 0.18189284896882185 1105 36-37 0.1010315375513855 1105 38-39 0.10429733713789346 1105 40-41 0.08425950270292049 1105 42-43 0.20292388721232868 1105 44-45 0.29372320394870144 1105 46-47 0.36008083042643335 1105 48-49 0.2761046216561738 1105 50-51 0.34270737540205687 1105 52-53 0.18908517748784703 1105 54-55 0.28910860322653775 1105 56-57 0.1856862907594845 1105 58-59 0.29272723997397776 1105 60-61 0.10784580810713607 1105 62-63 0.0696753896514366 1105 64-65 -0.043510074908887475 1105 66-67 0.14354931041486907 1105 68-69 0.17013039887364556 1105 70-71 -0.01959637861862973 1105 72-73 -0.030004135997401704 1105 74-75 0.219540059941167 1105 76-77 -0.14346427191654598 1105 78-79 0.16453909720870286 1105 80-81 0.29522070584453175 1105 82-83 0.35711673810684275 1105 84-85 0.3789754905154332 1105 86-87 0.2605453387305232 1105 88-89 0.341476052223868 1105 90-91 0.38977896116453437 1105 92-93 0.30341000405700314 1105 94-95 0.03810800014654703 1105 96-97 0.18377943329234014 1105 98-99 0.2609749347628494 1105 100-101 0.49959304801508253 1106 1 0.16610005074356593 1106 2 0.15384864157742584 1106 3 0.12543491557183728 1106 4 0.1777959595384857 1106 5 0.08405040038336864 1106 6 0.15208398955955715 1106 7 0.16584348961772122 1106 8 0.17446765700749012 1106 9 0.2224731082760485 1106 10-11 0.14331647862157837 1106 12-13 0.22780461372621374 1106 14-15 0.2738184911958186 1106 16-17 0.23027623400670194 1106 18-19 0.01459087119226865 1106 20-21 0.21569689547707327 1106 22-23 0.1846107941226549 1106 24-25 0.2265508619878247 1106 26-27 0.24120941501999482 1106 28-29 0.1783018060176147 1106 30-31 0.21208926710652776 1106 32-33 0.09974971553382517 1106 34-35 0.1402864638562349 1106 36-37 0.25282682955259617 1106 38-39 0.2561336745699947 1106 40-41 0.3930897179642443 1106 42-43 0.2837153535785504 1106 44-45 0.05342329364614784 1106 46-47 0.25852025050846805 1106 48-49 0.4083351759753384 1106 50-51 0.4096588747652845 1106 52-53 0.2618024929610243 1106 54-55 0.14836080819461728 1106 56-57 0.2158220469531429 1106 58-59 0.31660212446310254 1106 60-61 0.26090861313933544 1106 62-63 0.35879814963156065 1106 64-65 0.18966211431381197 1106 66-67 0.23934796537994885 1106 68-69 0.3188155266956514 1106 70-71 0.13041801866248193 1106 72-73 0.2285419118296872 1106 74-75 0.2521743155289329 1106 76-77 0.3589966966401441 1106 78-79 0.48841553816064476 1106 80-81 0.46694976784221254 1106 82-83 0.5898443312554953 1106 84-85 0.6059169331204863 1106 86-87 0.4100191162231148 1106 88-89 0.457616797633996 1106 90-91 0.3788189524684249 1106 92-93 0.3246717883006305 1106 94-95 0.33416555512922486 1106 96-97 0.5055469926406424 1106 98-99 0.4158788846889294 1106 100-101 0.6304737473990976 1107 1 0.17355078949799463 1107 2 0.20603917310646125 1107 3 0.18383905501449505 1107 4 0.13845099379850723 1107 5 0.11615917806321363 1107 6 0.1340772610777634 1107 7 0.117913390138348 1107 8 0.1547055789143812 1107 9 0.16680675993125504 1107 10-11 0.1351964038203235 1107 12-13 0.11534528697099944 1107 14-15 0.19563566867870463 1107 16-17 0.24318965324473396 1107 18-19 0.25935092443333474 1107 20-21 0.18159814816164754 1107 22-23 0.2465434323722704 1107 24-25 0.22460318129756018 1107 26-27 0.26623431651901086 1107 28-29 0.1960845464416181 1107 30-31 0.2141378917481802 1107 32-33 0.27680170298284423 1107 34-35 0.28031244886014406 1107 36-37 0.2531592875605142 1107 38-39 0.3822898411941793 1107 40-41 0.364216880822795 1107 42-43 0.4204801919212926 1107 44-45 0.3819452042176934 1107 46-47 0.3904971201278258 1107 48-49 0.3065130719497944 1107 50-51 0.13465231042193437 1107 52-53 0.28513703125056367 1107 54-55 0.3816230467229005 1107 56-57 0.35121440593802333 1107 58-59 0.44410448917968637 1107 60-61 0.41672067915500577 1107 62-63 0.47654849242288577 1107 64-65 0.4383740336561317 1107 66-67 0.5371843482559342 1107 68-69 0.37871817611002 1107 70-71 0.41973640448627236 1107 72-73 0.5460669047158291 1107 74-75 0.5456664052264131 1107 76-77 0.6126666961694696 1107 78-79 0.6525200601868555 1107 80-81 0.839464485948163 1107 82-83 0.7748786557827714 1107 84-85 0.751644399742684 1107 86-87 0.7048587180762809 1107 88-89 0.5995353921310311 1107 90-91 0.7073548398947764 1107 92-93 0.7099479721462068 1107 94-95 0.7137065695526239 1107 96-97 0.7700104689687493 1107 98-99 0.6859492300797072 1107 100-101 0.5206069480200064 1108 1 0.07241957361600271 1108 2 0.027717862090284484 1108 3 -0.018919827186451244 1108 4 0.11280305110754796 1108 5 0.1027745978368273 1108 6 0.13469949431685535 1108 7 0.11365128826626147 1108 8 0.08792619918485656 1108 9 0.1320973873155964 1108 10-11 0.07725015592591689 1108 12-13 0.09034998654822601 1108 14-15 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-0.8277542940965148 2316 56-57 -0.8642344921827103 2316 58-59 -0.7546644158973237 2316 60-61 -0.6950638619885297 2316 62-63 -0.8429967364588506 2316 64-65 -0.7728451161048184 2316 66-67 -0.9756976273252178 2316 68-69 -1.0397149236959606 2316 70-71 -0.9210345612404893 2316 72-73 -0.8081443113978537 2316 74-75 -0.9142002306098505 2316 76-77 -0.5808027880463023 2316 78-79 -0.8436407530751424 2316 80-81 -0.9527964432193095 2316 82-83 -0.7399071862612843 2316 84-85 -0.920657033190988 2316 86-87 -0.9791632068095879 2316 88-89 -1.0541753804717473 2316 90-91 -0.9976511496883056 2316 92-93 -1.0072792200752048 2316 94-95 -0.9521269151800134 2316 96-97 -0.559148453664104 2316 98-99 -0.9858959404120995 2316 100-101 -1.0456919802672466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 11.0 5 50.0 6 68.0 7 181.0 8 456.0 9 856.0 10 1520.0 11 2482.0 12 3815.0 13 5453.0 14 6978.0 15 9537.0 16 12989.0 17 17670.0 18 15649.0 19 9630.0 20 8682.0 21 9036.0 22 9658.0 23 10902.0 24 12635.0 25 15116.0 26 18694.0 27 23469.0 28 29596.0 29 37123.0 30 46681.0 31 59300.0 32 75903.0 33 100085.0 34 137299.0 35 196216.0 36 302440.0 37 454421.0 38 315703.0 39 20235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94746876790918 19.637575259808457 15.062672302512322 22.35228366977004 2 15.400187979845962 21.066176619854406 39.771490806039715 23.762144594259915 3 19.413227724540903 25.260499263401005 30.129106545759214 25.19716646629888 4 12.904990510215475 17.406396216265595 37.81410171831072 31.87451155520822 5 13.643890395804206 37.8218716141981 34.06108462379697 14.473153366200723 6 30.984984887452626 36.94819918114149 17.766276183071916 14.300539748333966 7 27.882446913244436 33.29449472938492 21.02577557105942 17.797282786311218 8 26.331101805183128 34.502432825792624 19.91009607479747 19.256369294226783 9 26.444065305691616 16.798728069552226 20.61005365005065 36.14715297470551 10-11 24.625614628461918 26.918112811476043 27.54723148197022 20.90904107809182 12-13 25.926112939141806 24.386338216577315 27.989371484480458 21.69817735980042 14-15 22.966682364499622 25.525640583228626 26.73213238144922 24.775544670822534 16-17 21.91963859799406 28.82071383188839 27.091524794118182 22.168122775999365 18-19 21.765943820589644 27.464319497560062 29.563562143245726 21.206174538604568 20-21 22.929881679250823 26.23934076736072 29.518681108440937 21.312096444947525 22-23 22.409270351591502 26.32183657219473 29.35992797756099 21.90896509865278 24-25 22.30763628446207 26.442767517122075 29.477553707196712 21.77204249121914 26-27 22.281804977715886 26.505687498667825 29.545304603691925 21.667202919924364 28-29 21.90509248863065 26.784974368694463 29.579995732133256 21.72993741054163 30-31 22.214220864867222 26.469691179746857 29.48468890367364 21.831399051712282 32-33 22.115097438723822 26.56914547410266 29.39774725842177 21.91800982875175 34-35 22.472657352168028 26.476315217898776 29.2885786085901 21.762448821343096 36-37 22.49809555372061 26.30703886389946 29.373819078364725 21.821046504015197 38-39 22.236241509230947 26.66345734396487 29.34718366706821 21.75311747973597 40-41 22.391601829868137 26.631995694547317 29.176242162936823 21.800160312647723 42-43 22.207299695313182 26.746417747753416 28.86060121346955 22.18568134346385 44-45 22.414983040250732 26.615185217513098 28.756746854174576 22.213084888061594 46-47 22.578650455192832 26.92390937807102 28.29155583613312 22.20588433060303 48-49 22.60692252473445 26.97527328994121 28.183075143347015 22.234729041977324 50-51 22.49052034266078 27.092750292008716 28.204603777855652 22.212125587474848 52-53 22.67534208758173 27.392735421821747 27.727870622791222 22.2040518678053 54-55 22.522142363247674 27.051148510203692 27.93945660655041 22.487252519998226 56-57 22.559909260687753 27.36406865332305 27.70662478177906 22.369397304210143 58-59 22.830105376938867 27.029248253655712 27.847930394890753 22.292715974514664 60-61 22.67062297975434 27.080191715746317 28.24138178649662 22.00780351800272 62-63 22.54447100928168 27.044910251055477 28.180163729845248 22.2304550098176 64-65 22.447512062634353 27.14445423303568 28.101954380202866 22.306079324127097 66-67 22.522542410383515 27.140590142237432 27.766976840489864 22.56989060688919 68-69 22.40854161369898 27.16363171332137 27.980640849391108 22.447185823588548 70-71 22.65046355619588 26.960934815914726 27.913370382643834 22.47523124524556 72-73 22.521197149219404 26.978637414566915 27.90315181360718 22.597013622606504 74-75 22.415363645036813 27.45606805822543 27.56773750246124 22.560830794276516 76-77 22.58069836410988 27.15615148679454 27.609045808577104 22.654104340518476 78-79 22.48546954936192 27.211107541827857 27.62597328518353 22.677449623626693 80-81 22.567585706590123 27.404870753429368 27.401293011248573 22.626250528731934 82-83 22.597721088935998 27.235073162982594 27.602347224597747 22.564858523483664 84-85 22.56477560249868 27.23718070227926 27.38415604762184 22.81388764760022 86-87 22.381585778468867 27.150811707013073 27.509734952501347 22.95786756201671 88-89 22.478561150421978 27.03602782273851 27.448324614516835 23.037086412322676 90-91 22.432049082992478 27.02066438641185 27.644503537101507 22.90278299349417 92-93 22.497024934726177 26.94731660530462 27.621165718113517 22.93449274185568 94-95 22.308312205538762 26.965544609925768 27.661061796008013 23.065081388527457 96-97 22.038310396372104 26.921362216218697 27.799942096193902 23.240385291215294 98-99 22.089239873424308 26.882719565718805 27.491086745862326 23.536953814994565 100-101 21.985812543595205 26.918436461818786 27.48135222592143 23.614398768664586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 387.0 1 293.0 2 219.5 3 242.5 4 211.5 5 205.5 6 345.0 7 723.5 8 1270.0 9 1625.0 10 1542.5 11 1398.0 12 1504.5 13 1708.5 14 1941.5 15 2119.5 16 2241.0 17 2435.0 18 2639.0 19 2901.0 20 3266.5 21 3713.5 22 4317.0 23 5144.0 24 6271.0 25 7957.5 26 10339.5 27 13135.5 28 15678.5 29 18888.5 30 22731.5 31 26387.0 32 30150.5 33 33758.0 34 38593.0 35 43168.0 36 48252.0 37 54130.0 38 56949.0 39 58360.5 40 60364.5 41 63050.0 42 64568.0 43 66682.5 44 70234.0 45 71842.5 46 73000.5 47 77203.0 48 85974.0 49 85172.5 50 75582.0 51 70448.5 52 67519.0 53 65225.0 54 62973.5 55 59504.5 56 54222.0 57 48511.0 58 44100.0 59 39495.5 60 33382.0 61 26783.0 62 20847.5 63 16019.0 64 11909.0 65 8751.5 66 6294.0 67 4677.0 68 3241.5 69 2006.5 70 1329.0 71 872.5 72 594.0 73 441.5 74 297.5 75 201.5 76 139.5 77 64.0 78 29.0 79 16.5 80 8.0 81 5.5 82 5.0 83 3.0 84 2.5 85 2.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02735279729293577 2 0.006292665796519547 3 2.537365240532075E-4 4 4.0597843848513203E-4 5 1.5224191443192453E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012788320812281661 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.0298921924256601E-4 20-21 7.358359197543018E-4 22-23 0.0025881125453427168 24-25 0.01073305496745068 26-27 0.00436426821371517 28-29 4.313520908904528E-4 30-31 0.002486617935721434 32-33 0.005303093352712038 34-35 0.0 36-37 0.008068821464892 38-39 0.012509210635823132 40-41 0.0011925616630500753 42-43 0.0 44-45 0.0 46-47 4.820993957010943E-4 48-49 0.003425443074718302 50-51 0.007332985545137697 52-53 0.0 54-55 0.0030702119410438112 56-57 0.0034508167271236224 58-59 1.5224191443192453E-4 60-61 0.001344803577482 62-63 0.005429961614738641 64-65 0.0 66-67 0.00202989219242566 68-69 3.5523113367449055E-4 70-71 0.0010656934010234717 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0015731664491298867 80-81 0.0015985401015352076 82-83 0.011215154363151774 84-85 0.007586722069190905 86-87 0.003603058641555547 88-89 7.612095721596227E-5 90-91 4.0597843848513203E-4 92-93 1.5224191443192453E-4 94-95 0.0 96-97 0.001953771235209698 98-99 0.0013955508822926415 100-101 2.283628716478868E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1970548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.88208316976001 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35737782484982 41.552451718935274 2 14.439321078455 16.137986828421823 3 4.848346271030691 8.128070686605986 4 2.1884840355978508 4.891881875718846 5 1.1817430448377872 3.3019131558455337 6 0.6999560910183414 2.3469002696080246 7 0.46033800067505287 1.800725250794704 8 0.3207522228224909 1.4339441914121467 9 0.2360192987266865 1.1870325073001817 >10 1.1689383040875367 11.660734078379893 >50 0.06529918641784542 2.5056775679514955 >100 0.03105770714176131 2.974376288184416 >500 0.0011834671696310374 0.4290610057723732 >1k 0.0010013952973801025 0.9942575642417859 >5k 1.8207187225092775E-4 0.654987010827517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7242 0.3675119814386658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5633 0.2858595679983436 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4002 0.2030907138521873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3580 0.18167535122209658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.0747304810641504E-5 0.0 16-17 0.0 0.0 0.0 5.0747304810641504E-5 0.0 18-19 0.0 0.0 0.0 5.0747304810641504E-5 0.0 20-21 0.0 0.0 0.0 7.612095721596225E-5 0.0 22-23 0.0 0.0 0.0 2.791101764585283E-4 0.0 24-25 0.0 0.0 0.0 4.5672574329577357E-4 0.0 26-27 0.0 0.0 0.0 6.850886149436604E-4 0.0 28-29 0.0 0.0 0.0 0.001446298187103283 0.0 30-31 0.0 0.0 0.0 0.0029687173314225284 0.0 32-33 0.0 0.0 0.0 0.004465762823336453 0.0 34-35 0.0 0.0 0.0 0.006089676577276981 0.0 36-37 0.0 0.0 0.0 0.008018074160081358 0.0 38-39 0.0 0.0 0.0 0.009997219047696376 0.0 40-41 0.0 0.0 0.0 0.013194299250766792 0.0 42-43 0.0 0.0 0.0 0.017786930336129846 0.0 44-45 0.0 0.0 0.0 0.02737817094534109 0.0 46-47 0.0 0.0 0.0 0.04760097191238173 0.0 48-49 0.0 0.0 0.0 0.06384010945178702 0.0 50-51 0.0 0.0 0.0 0.0812971823066477 0.0 52-53 0.0 0.0 0.0 0.10177371979774155 0.0 54-55 0.0 0.0 0.0 0.12367118182353334 0.0 56-57 0.0 0.0 0.0 0.1504911324159574 0.0 58-59 0.0 0.0 0.0 0.1932964840237335 0.0 60-61 0.0 0.0 0.0 0.24417065709640162 0.0 62-63 0.0 0.0 0.0 0.31072574735555797 0.0 64-65 0.0 0.0 0.0 0.3628939767008974 0.0 66-67 0.0 0.0 0.0 0.4433284548257642 0.0 68-69 0.0 0.0 0.0 0.5491619590083571 0.0 70-71 0.0 0.0 0.0 0.6161737750108092 0.0 72-73 0.0 0.0 0.0 0.7268028994980076 0.0 74-75 0.0 0.0 0.0 0.8207615343549104 0.0 76-77 0.0 0.0 0.0 0.8990899993301356 0.0 78-79 0.0 0.0 0.0 1.0001025095557174 0.0 80-81 0.0 0.0 0.0 1.1040329898079113 0.0 82-83 0.0 0.0 0.0 1.2405686134009422 0.0 84-85 0.0 0.0 0.0 1.4438115691675615 0.0 86-87 0.0 0.0 0.0 1.7201052702090993 0.0 88-89 0.0 0.0 0.0 2.0449387683020155 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14625 0.0 24.525345 1 GTACATG 9485 0.0 23.991766 1 TACATGG 9515 0.0 23.810825 2 ACATGGG 9610 0.0 22.436972 3 GAGTACT 7180 0.0 21.166904 12-13 TCAACGC 16920 0.0 21.051966 4 ATCAACG 16965 0.0 20.968132 3 TATCAAC 17000 0.0 20.954493 2 CAACGCA 17080 0.0 20.859524 5 AGTACTT 7555 0.0 20.807758 12-13 AACGCAG 17475 0.0 20.442389 6 GGTATCA 10855 0.0 20.39483 1 GTACTTT 7620 0.0 19.913504 14-15 GTCTACG 125 0.006026992 19.00308 1 AGAGTAC 13775 0.0 18.639534 10-11 CATGGGA 5910 0.0 18.482985 4 ACGCAGA 19265 0.0 18.444359 7 CGCAGAG 19240 0.0 18.270802 8 CATGGGG 4215 0.0 17.802921 4 TACTTTT 8945 0.0 17.441618 14-15 >>END_MODULE