FastQCFastQC Report
Thu 26 May 2016
SRR936888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961352
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62500.3186577422104752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.22754712055765616No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35910.18308799236445064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.14571581235800612No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20840.10625323756266085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132800.026.3463541
GTACATG99100.023.6970391
TACATGG98700.023.2174762
TATCAAC153400.022.5317122
TCAACGC153850.022.4972864
ATCAACG154150.022.452933
CAACGCA155250.022.2637885
AACGCAG158000.021.9030196
ACATGGG101750.021.7271943
GAGTACT58050.021.5327812-13
GGTATCA97550.020.321211
GTACTTT61700.020.180914-15
ACGCAGA174050.019.8255757
CATGGGA60800.019.7839574
CGCAGAG173300.019.6091868
AGTACTT59200.019.26797512-13
AGAGTAC124300.018.77184910-11
GCAGAGT177100.018.4883169
CATGGGG44450.017.9694084
CGCGGAA2002.1827873E-1117.81582882-83
Produced by FastQC (version 0.11.5)