##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936888_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1961352 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.718696083110018 33.0 31.0 34.0 30.0 34.0 2 31.79633793424128 33.0 31.0 34.0 30.0 34.0 3 31.824310985483482 33.0 31.0 34.0 30.0 34.0 4 35.42120741203007 37.0 35.0 37.0 33.0 37.0 5 35.43468892886131 37.0 35.0 37.0 33.0 37.0 6 35.49695414183685 37.0 35.0 37.0 33.0 37.0 7 35.43617820768531 37.0 35.0 37.0 33.0 37.0 8 35.40672556481447 37.0 35.0 37.0 33.0 37.0 9 37.018625927421496 39.0 37.0 39.0 33.0 39.0 10-11 36.88091046380252 39.0 37.0 39.0 33.0 39.0 12-13 36.80084706875665 39.0 37.0 39.0 33.0 39.0 14-15 37.93890974185154 40.0 38.0 41.0 33.0 41.0 16-17 37.87156079071987 40.0 38.0 41.0 33.0 41.0 18-19 37.83296955365482 40.0 38.0 41.0 32.5 41.0 20-21 37.76223722207946 40.0 37.5 41.0 32.0 41.0 22-23 37.6541472412907 40.0 37.0 41.0 32.0 41.0 24-25 37.611572017669445 40.0 37.0 41.0 32.0 41.0 26-27 37.643110211731496 40.0 37.0 41.0 32.0 41.0 28-29 37.57875409411467 40.0 37.0 41.0 32.0 41.0 30-31 37.43529412364532 40.0 37.0 41.0 32.0 41.0 32-33 37.27104160803364 40.0 36.5 41.0 31.0 41.0 34-35 37.12057295171902 39.5 36.0 41.0 31.0 41.0 36-37 36.95119820409595 39.0 36.0 41.0 30.5 41.0 38-39 36.79961730479791 39.0 36.0 41.0 30.0 41.0 40-41 36.635211833469974 39.0 36.0 41.0 30.0 41.0 42-43 36.42577900346292 39.0 35.0 41.0 30.0 41.0 44-45 36.19707706724749 39.0 35.0 40.5 29.5 41.0 46-47 35.881572507127736 39.0 35.0 40.0 28.5 41.0 48-49 35.662821614886056 39.0 35.0 40.0 27.5 41.0 50-51 34.97978129371984 38.0 34.0 39.5 26.5 40.5 52-53 35.160760536609445 38.5 34.5 40.0 27.5 40.5 54-55 35.556446267676584 39.0 35.0 40.5 28.0 41.0 56-57 35.444808733975336 39.0 35.0 41.0 27.5 41.0 58-59 35.23866980531796 38.5 35.0 40.0 27.0 41.0 60-61 34.943665899848675 38.0 34.0 40.0 26.0 41.0 62-63 34.63446974331991 38.0 34.0 40.0 26.0 41.0 64-65 34.30391587027724 37.0 34.0 40.0 26.0 41.0 66-67 33.93079085243241 37.0 33.5 39.5 25.0 41.0 68-69 33.54465950018151 36.0 33.0 39.0 24.0 41.0 70-71 33.135715822555056 36.0 33.0 39.0 23.0 40.5 72-73 32.745267805065076 35.0 32.5 38.5 22.5 40.0 74-75 32.349912968197444 35.0 32.0 37.0 22.0 39.5 76-77 31.899943763281655 35.0 32.0 37.0 20.5 39.0 78-79 31.44725628036171 35.0 31.0 36.0 19.5 39.0 80-81 31.026494734244544 35.0 31.0 36.0 18.0 37.5 82-83 30.632511145373194 34.5 31.0 35.5 16.0 37.0 84-85 30.272967830353757 34.0 30.5 35.0 12.0 37.0 86-87 29.93724507380623 34.0 30.0 35.0 7.5 36.0 88-89 29.579914518148705 34.0 30.0 35.0 4.5 36.0 90-91 29.230335758191288 34.0 29.5 35.0 2.0 35.5 92-93 28.86937428875592 34.0 29.0 35.0 2.0 35.0 94-95 28.508405936313316 34.0 29.0 35.0 2.0 35.0 96-97 28.029113591033123 34.0 29.0 35.0 2.0 35.0 98-99 27.48296404724904 34.0 28.0 35.0 2.0 35.0 100-101 25.30536308627926 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4271707569990646 1101 2 0.394818123217874 1101 3 0.4229618254638474 1101 4 0.3649861389534479 1101 5 0.4127672760947618 1101 6 0.374455357241942 1101 7 0.26957495313428836 1101 8 0.3642100701178279 1101 9 0.5333351323269753 1101 10-11 0.4735466984801988 1101 12-13 0.5352374204566246 1101 14-15 0.6140875578198006 1101 16-17 0.840965367422811 1101 18-19 0.7694355892089249 1101 20-21 0.7203299511835866 1101 22-23 0.7581308681660133 1101 24-25 0.7814533746248316 1101 26-27 0.800713050681324 1101 28-29 0.7181091445910681 1101 30-31 0.6692106953727404 1101 32-33 0.5445356199504445 1101 34-35 0.6595956026723186 1101 36-37 0.5878566450568457 1101 38-39 0.5412373478803616 1101 40-41 0.5827771074758417 1101 42-43 0.6223372170956196 1101 44-45 0.7173821104298597 1101 46-47 0.7504039499834718 1101 48-49 0.8995671029487795 1101 50-51 0.810991769352178 1101 52-53 0.9000905769771776 1101 54-55 0.8970995500198882 1101 56-57 0.8154392656456366 1101 58-59 0.8911007542377973 1101 60-61 1.076911227522281 1101 62-63 1.0245292148528762 1101 64-65 0.8374868615981796 1101 66-67 0.8146898108715632 1101 68-69 0.8323325888550386 1101 70-71 0.7894008090407283 1101 72-73 0.7289292287840823 1101 74-75 0.7978922259916388 1101 76-77 0.8420098695185452 1101 78-79 0.7892480846272001 1101 80-81 0.741815721928571 1101 82-83 0.7596983047109198 1101 84-85 0.7347068318612457 1101 86-87 0.8860048420733335 1101 88-89 0.9537328784450736 1101 90-91 1.0887163281714862 1101 92-93 1.1591294548330673 1101 94-95 1.2865422973220717 1101 96-97 1.3842898481289687 1101 98-99 1.4564356672833085 1101 100-101 1.5098302156715526 1102 1 0.2873898693982291 1102 2 0.23468492880108016 1102 3 0.23526938254070373 1102 4 0.2376646514434384 1102 5 0.22513343319970858 1102 6 0.25532580895336565 1102 7 0.2816017471353689 1102 8 0.2132090006673124 1102 9 0.3797444586937999 1102 10-11 0.33034158717892836 1102 12-13 0.3452182117121225 1102 14-15 0.3345378473297913 1102 16-17 0.4576272512930686 1102 18-19 0.2628055992657252 1102 20-21 0.311391378663032 1102 22-23 0.3750823051162655 1102 24-25 0.30249293912321207 1102 26-27 0.31223011061450734 1102 28-29 0.3014013286399688 1102 30-31 0.2885474981724343 1102 32-33 0.18110470895268094 1102 34-35 0.1511171265488187 1102 36-37 0.3339655601031808 1102 38-39 0.4411398822454018 1102 40-41 0.16531998675374382 1102 42-43 0.39063356777887037 1102 44-45 0.5749331980205312 1102 46-47 0.5662714587670123 1102 48-49 0.6964064070217404 1102 50-51 0.5857397245989873 1102 52-53 0.6979079844602296 1102 54-55 0.5173692135609329 1102 56-57 0.6906487822901966 1102 58-59 0.6042915343851476 1102 60-61 0.7346215693069453 1102 62-63 0.6247352628928269 1102 64-65 0.6363500210531612 1102 66-67 0.2544481202262787 1102 68-69 0.5242485426265162 1102 70-71 0.5933957797794918 1102 72-73 0.533851027908355 1102 74-75 0.6122843928914108 1102 76-77 0.5157102630866781 1102 78-79 0.5426376222815996 1102 80-81 0.5094153355130864 1102 82-83 0.5504850014704239 1102 84-85 0.45033037493959327 1102 86-87 0.3755938576704203 1102 88-89 0.4191476589233396 1102 90-91 0.3146629556106042 1102 92-93 0.4270444744511508 1102 94-95 0.4761228676209903 1102 96-97 0.3507477952838087 1102 98-99 0.46275585266622343 1102 100-101 0.46560184446795105 1103 1 0.3663132246379597 1103 2 0.3554831465279378 1103 3 0.3245196224907083 1103 4 0.24127240467174005 1103 5 0.19762979541951609 1103 6 0.19470721094162968 1103 7 0.3300697872832856 1103 8 0.3090279715448787 1103 9 0.31335483728866365 1103 10-11 0.37254714487800555 1103 12-13 0.3499474588271454 1103 14-15 0.627147839028531 1103 16-17 0.5570563669271777 1103 18-19 0.6213290325224179 1103 20-21 0.4797290592632777 1103 22-23 0.5091319808781734 1103 24-25 0.5246426626094021 1103 26-27 0.6633235970481621 1103 28-29 0.6877803529176987 1103 30-31 0.5805889704610081 1103 32-33 0.6103310176766499 1103 34-35 0.6884454281067107 1103 36-37 0.5339940458619026 1103 38-39 0.6435997550586166 1103 40-41 0.695185790131859 1103 42-43 0.8069668819799816 1103 44-45 0.7082000468685052 1103 46-47 0.5594540089014188 1103 48-49 0.6538390642434706 1103 50-51 0.5996643978365128 1103 52-53 0.7171641713438106 1103 54-55 0.784487772776771 1103 56-57 0.8619681671678734 1103 58-59 0.8352047474192901 1103 60-61 0.7933935089213833 1103 62-63 0.812794149758659 1103 64-65 0.7165663959002444 1103 66-67 0.9049946907216224 1103 68-69 0.8993014219733837 1103 70-71 0.7457351992864929 1103 72-73 0.7370364638201821 1103 74-75 0.6325018412689474 1103 76-77 0.6382867149303273 1103 78-79 0.7848959796099315 1103 80-81 0.7126066173828605 1103 82-83 0.9013996547360321 1103 84-85 0.8314691622328212 1103 86-87 0.7700080182812066 1103 88-89 0.6610787754694165 1103 90-91 0.7436210145825015 1103 92-93 0.6974548394408764 1103 94-95 0.9507919332562196 1103 96-97 0.8589221717061051 1103 98-99 0.9415654508788478 1103 100-101 0.9605910613327957 1104 1 0.21970033173282388 1104 2 0.17219614536903904 1104 3 0.2346837876939425 1104 4 0.31627542633528805 1104 5 0.39164903452908817 1104 6 0.1819717878020839 1104 7 0.21611219011013816 1104 8 0.3246304201588259 1104 9 0.35534707339078864 1104 10-11 0.4223717106471767 1104 12-13 0.2835659542519906 1104 14-15 0.16746838420773003 1104 16-17 0.4330757340130873 1104 18-19 0.3980165427427522 1104 20-21 0.3066206206457025 1104 22-23 0.3289821774640558 1104 24-25 0.3400803416874041 1104 26-27 0.38491907095165345 1104 28-29 0.38504165927172096 1104 30-31 0.4826461901039494 1104 32-33 0.2592288214604892 1104 34-35 0.3454812828109084 1104 36-37 0.3867288291197468 1104 38-39 0.5770236198018424 1104 40-41 0.49818665752704305 1104 42-43 0.5767809963481625 1104 44-45 0.5091808433079166 1104 46-47 0.28308555080300124 1104 48-49 0.4151614153110046 1104 50-51 0.3126641276642417 1104 52-53 0.47865271390660524 1104 54-55 0.6109571899867419 1104 56-57 0.6628467602813259 1104 58-59 0.6413668081227328 1104 60-61 0.754846857991069 1104 62-63 0.6379304801082526 1104 64-65 0.4776256844939937 1104 66-67 0.3895086650268169 1104 68-69 0.44348258670026297 1104 70-71 0.6215812650805574 1104 72-73 0.5524172645254737 1104 74-75 0.5657657355889327 1104 76-77 0.5434953519112291 1104 78-79 0.6708139609964796 1104 80-81 0.5389756087160187 1104 82-83 0.5526312492501155 1104 84-85 0.5871934372113117 1104 86-87 0.7154163338992952 1104 88-89 0.7463954458094797 1104 90-91 0.5372253970611176 1104 92-93 0.5785132748590414 1104 94-95 0.6822309205367922 1104 96-97 0.7246687714458844 1104 98-99 0.6974372541365561 1104 100-101 0.8448382405210033 1105 1 0.12605802579495062 1105 2 0.2497949366393044 1105 3 0.20416425528253868 1105 4 0.1886157568200275 1105 5 0.19733500801267922 1105 6 0.24173124303982263 1105 7 0.25165287479557463 1105 8 0.16091807321685536 1105 9 0.2895744954539765 1105 10-11 0.2028383809868899 1105 12-13 0.21250406691223134 1105 14-15 0.3252971577530701 1105 16-17 0.4034083233065573 1105 18-19 0.29021328197192275 1105 20-21 0.4190941089517679 1105 22-23 0.4042247597737756 1105 24-25 0.39052453199681736 1105 26-27 0.4179865304004906 1105 28-29 0.40009010128004263 1105 30-31 0.3373057707712235 1105 32-33 0.28131983158270657 1105 34-35 0.08263356578232361 1105 36-37 0.1979700355265379 1105 38-39 0.47941355625319915 1105 40-41 0.3777074194159198 1105 42-43 0.426992097184673 1105 44-45 0.39107853708215146 1105 46-47 0.338555856961257 1105 48-49 0.31936367701349866 1105 50-51 0.1746529228904805 1105 52-53 0.2946044768939231 1105 54-55 0.19098081163924263 1105 56-57 0.4028192223682936 1105 58-59 0.41001694409986555 1105 60-61 0.4198580483704646 1105 62-63 0.40530199921528265 1105 64-65 0.4036159300767821 1105 66-67 0.3008845332180954 1105 68-69 0.3647071800221653 1105 70-71 0.2910822570509808 1105 72-73 0.19279634266057855 1105 74-75 0.2836041285422439 1105 76-77 0.1513473798045375 1105 78-79 0.22595778802185507 1105 80-81 0.11988287077340942 1105 82-83 0.10514103121516172 1105 84-85 0.21574853562162843 1105 86-87 0.18205478264318842 1105 88-89 0.4034887416578954 1105 90-91 0.26103968590245685 1105 92-93 0.22819434035985964 1105 94-95 0.35438730093920867 1105 96-97 0.22877575732997713 1105 98-99 0.49469271990104247 1105 100-101 0.7931231268824384 1106 1 0.3249512315411671 1106 2 0.36289959644696523 1106 3 0.3697217294413555 1106 4 0.21540275588470337 1106 5 0.23443599849160535 1106 6 0.3754144216857682 1106 7 0.289419380779222 1106 8 0.39186544353126607 1106 9 0.41510660807598754 1106 10-11 0.438026913735051 1106 12-13 0.476904833154407 1106 14-15 0.3568589307823018 1106 16-17 0.4484535303957955 1106 18-19 0.47748170056318173 1106 20-21 0.3786070835062958 1106 22-23 0.22773424486862126 1106 24-25 0.264998415009984 1106 26-27 0.24343485402089016 1106 28-29 0.1801941855067355 1106 30-31 0.30717920291262146 1106 32-33 0.39643227972046446 1106 34-35 0.4971170717899511 1106 36-37 0.4472745418459283 1106 38-39 0.1847041047339033 1106 40-41 0.28533491659796795 1106 42-43 0.1399398212415619 1106 44-45 0.2208204849441131 1106 46-47 0.40275262171651605 1106 48-49 0.4740765460448486 1106 50-51 0.5439188472975047 1106 52-53 0.47842840307566803 1106 54-55 0.5550102981730518 1106 56-57 0.33596714068376343 1106 58-59 0.42867461131812945 1106 60-61 0.6479072785309157 1106 62-63 0.5957428582698867 1106 64-65 0.3585647273523662 1106 66-67 0.29988026432585 1106 68-69 0.3683750098420546 1106 70-71 0.5154449210316017 1106 72-73 0.4976264263596448 1106 74-75 0.6690447777496189 1106 76-77 0.7102499986979787 1106 78-79 0.634385702500115 1106 80-81 0.8568603511676827 1106 82-83 0.796855213290609 1106 84-85 0.8778030599279347 1106 86-87 1.1190264172931634 1106 88-89 1.0314146116245588 1106 90-91 0.9648830379421725 1106 92-93 1.0958676741728475 1106 94-95 1.3705155595669254 1106 96-97 1.4139367101563174 1106 98-99 1.3029650476464454 1106 100-101 1.5789035787135042 1107 1 0.3151695966496355 1107 2 0.378124659871645 1107 3 0.3687023294430283 1107 4 0.3659395631322866 1107 5 0.3874339136406846 1107 6 0.4091011958604156 1107 7 0.5069492929475459 1107 8 0.35704832849606305 1107 9 0.47574887193940896 1107 10-11 0.48773129820165195 1107 12-13 0.5909669537575937 1107 14-15 0.5542664207557593 1107 16-17 0.6847701263114985 1107 18-19 0.6311073212040483 1107 20-21 0.7381386828130161 1107 22-23 0.6349719848567332 1107 24-25 0.6123338457535752 1107 26-27 0.6169876514052035 1107 28-29 0.5467720994836043 1107 30-31 0.7411646801257916 1107 32-33 0.7440636399810288 1107 34-35 0.5675501283990982 1107 36-37 0.5883054964397161 1107 38-39 0.7298277305324703 1107 40-41 0.8227609734666785 1107 42-43 0.7888766205348858 1107 44-45 0.7501800421854128 1107 46-47 0.802660415207427 1107 48-49 0.8203287334637395 1107 50-51 0.870247992089979 1107 52-53 0.8291551180539329 1107 54-55 0.8136809545243082 1107 56-57 0.9190788539455923 1107 58-59 0.8889287391554959 1107 60-61 1.077003769869151 1107 62-63 1.0977267111413767 1107 64-65 1.1264743503208905 1107 66-67 1.1098144952335574 1107 68-69 1.0470175332652332 1107 70-71 0.9188886928534643 1107 72-73 0.8819820902046516 1107 74-75 0.9605955321474866 1107 76-77 1.0012808486305822 1107 78-79 1.0832380402124748 1107 80-81 0.8882503355103673 1107 82-83 0.8048960709262722 1107 84-85 0.8880478205887243 1107 86-87 1.0722206245055723 1107 88-89 0.9880294082050689 1107 90-91 1.0451505833441992 1107 92-93 0.9716442602085316 1107 94-95 0.9946060538189343 1107 96-97 0.9064081690495875 1107 98-99 1.3176927087618822 1107 100-101 1.5286290524100572 1108 1 -0.01619166654812787 1108 2 0.052764979498125086 1108 3 0.02036741050511992 1108 4 0.0175271221140747 1108 5 0.028495887637426165 1108 6 -0.026663174304573545 1108 7 -0.1059711846080944 1108 8 -8.779978630002461E-5 1108 9 0.058150672806775106 1108 10-11 -0.18509755610541134 1108 12-13 -0.09375393486133277 1108 14-15 0.07190894976152862 1108 16-17 0.11665790181540814 1108 18-19 0.1073024001220162 1108 20-21 0.09434062096315188 1108 22-23 0.06448625995904678 1108 24-25 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-3.8397815597233382 2316 94-95 -4.297609049563782 2316 96-97 -4.504027642564132 2316 98-99 -4.972558717206589 2316 100-101 -5.174389495082618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3289.0 3 1264.0 4 1271.0 5 1392.0 6 1539.0 7 1836.0 8 2223.0 9 2904.0 10 3787.0 11 4712.0 12 6071.0 13 7459.0 14 8302.0 15 10134.0 16 12783.0 17 16559.0 18 14544.0 19 9999.0 20 9952.0 21 10853.0 22 11970.0 23 13214.0 24 15199.0 25 17830.0 26 20830.0 27 24634.0 28 29702.0 29 36137.0 30 44825.0 31 55675.0 32 70706.0 33 92204.0 34 125864.0 35 177330.0 36 266143.0 37 399285.0 38 364805.0 39 64110.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78391446427196 19.19983923542982 14.508747438962253 22.507498861335968 2 15.088174363797155 20.721102993001743 40.51634711598583 23.674375527215275 3 19.211921640944254 25.452464746533554 29.932616679016622 25.402996933505566 4 12.51711381939641 16.97787802922255 38.30174800704344 32.2032601443376 5 13.653399417814233 37.84529005180882 33.994127109198615 14.507183421178334 6 30.7549742158984 36.77063506525524 18.136306863220995 14.338083855625364 7 27.77494647303603 33.270381440516836 21.122298590928498 17.83237349551863 8 26.022204100961506 34.627211760539446 20.132800037557956 19.217784100941092 9 26.515147263288135 17.106011709631748 20.70293506129061 35.675905965789504 10-11 24.499113742049445 26.924945125479226 27.675126509878435 20.900814622592893 12-13 25.818463921565428 24.4853190282211 27.894075977370875 21.802141072842602 14-15 22.958148732401266 25.61739700951592 26.623552868142387 24.80090138994043 16-17 21.974864269401326 28.934172381303636 26.89814963600275 22.19281371329229 18-19 21.808792187103414 27.73537473359038 29.152884366426584 21.302948712879623 20-21 23.04255455370277 26.568518593624173 28.973016294498294 21.415910558174758 22-23 22.5447958659072 26.659533058053565 28.741905836366303 22.053765239672927 24-25 22.340993480342615 26.76827732356489 28.939533313684958 21.951195882407532 26-27 22.311081176095986 26.841145768935732 28.95626363423377 21.89150942073451 28-29 21.970898310823213 27.14099384455984 28.98464301302473 21.903464831592213 30-31 22.278558721537646 26.79490585249774 28.911357203715422 22.01517822224919 32-33 22.122457553984713 26.879302314138627 28.841314350994136 22.156925780882524 34-35 22.54066816455262 26.881919999836835 28.664468872126548 21.912942963483996 36-37 22.551070148030007 26.643463469751545 28.757118003858817 22.048348378359638 38-39 22.274656786726833 26.984334979111864 28.73446709942815 22.00654113473315 40-41 22.445189225791122 26.957753459358084 28.651689117184258 21.945368197666536 42-43 22.17543866805216 27.068217764131347 28.34376360719898 22.412579960617514 44-45 22.40168295851544 26.99366953074173 28.22305318695483 22.381594323788004 46-47 22.535823459665803 27.221368063693856 27.883779261869368 22.359029214770977 48-49 22.559788023122895 27.219419725025567 27.802182786718916 22.41860946513262 50-51 22.421068759348017 27.39513840611244 27.837699416946265 22.34609341759328 52-53 22.619199977972908 27.573639074841644 27.408920259369037 22.39824068781642 54-55 22.362428886045834 27.253707461018312 27.722905309382988 22.66095834355287 56-57 22.54844597345628 27.525858800787994 27.44705418346459 22.47864104229113 58-59 22.75869857787452 27.256865439951827 27.549917667492924 22.434518314680734 60-61 22.654601901235583 27.302021334281 27.856142611278674 22.187234153204745 62-63 22.462108918582455 27.299730952862316 27.86015871563071 22.37800141292451 64-65 22.408396450322616 27.3610172818608 27.744715890695225 22.485870377121355 66-67 22.42157234473261 27.44194692282802 27.37544682937601 22.761033903063364 68-69 22.305271319411148 27.419226492282267 27.687816896379346 22.58768529192724 70-71 22.520264253466614 27.22377495550754 27.59180547949189 22.66415531153396 72-73 22.446455207661735 27.205162346907898 27.599879364404394 22.748503081025973 74-75 22.216290045697306 27.75588056743903 27.387966639410948 22.639862747452714 76-77 22.3933120284197 27.337435714892216 27.41426293676837 22.85498931991972 78-79 22.410437323657245 27.445326574351142 27.434718916182927 22.709517185808686 80-81 22.477522732386706 27.70328016821533 27.159723430494587 22.65947366890338 82-83 22.501474532095838 27.586487395523697 27.30499392719726 22.60704414518321 84-85 22.459377550336196 27.518658167181968 27.168478921809957 22.853485360671876 86-87 22.365106934450548 27.419473706087665 27.404096627217534 22.811322732244253 88-89 22.494439228630913 27.3086478698439 27.190456770087707 23.00645613143748 90-91 22.448962415802363 27.273186341282273 27.422103079763417 22.855748163151944 92-93 22.527750963712386 27.266346768136472 27.4278347426126 22.778067525538535 94-95 22.3558087001749 27.2840126255029 27.370086328660808 22.990092345661388 96-97 22.11121440187086 27.336012887151245 27.548911970945777 23.003860740032117 98-99 22.175702980492947 27.237907200275835 27.322753740758245 23.263636078472977 100-101 22.109750239234693 27.302986307178113 27.383526315561213 23.203737138025982 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 299.0 1 213.0 2 156.5 3 202.0 4 198.5 5 216.5 6 361.5 7 642.5 8 1074.5 9 1355.5 10 1322.5 11 1325.0 12 1437.5 13 1588.0 14 1767.5 15 1904.5 16 2051.0 17 2186.0 18 2316.5 19 2541.5 20 2851.5 21 3312.0 22 3967.5 23 4866.0 24 5935.0 25 7482.0 26 10051.5 27 13010.5 28 15523.5 29 18733.0 30 22550.5 31 26334.5 32 30234.5 33 34177.0 34 39294.5 35 43737.5 36 48614.5 37 54078.5 38 56913.0 39 58670.5 40 60585.5 41 63232.0 42 65011.0 43 67081.0 44 70035.0 45 71488.5 46 73046.0 47 76724.0 48 84620.5 49 84158.0 50 75052.5 51 69887.5 52 67011.0 53 65192.0 54 62779.0 55 58926.5 56 54049.0 57 48281.5 58 43804.5 59 39382.5 60 33287.5 61 26840.5 62 20794.0 63 15778.5 64 11823.5 65 8724.5 66 6127.5 67 4589.5 68 3346.0 69 2100.0 70 1333.0 71 908.0 72 640.0 73 452.0 74 313.0 75 203.0 76 150.5 77 84.5 78 41.5 79 20.5 80 14.0 81 13.0 82 14.5 83 12.0 84 11.0 85 7.5 86 2.5 87 2.0 88 2.5 89 3.5 90 4.0 91 3.0 92 1.5 93 2.5 94 3.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03676035714140042 2 0.029469467999624747 3 0.02452389984051817 4 0.048334006338484885 5 0.0938128393067639 6 0.1121165400193336 7 0.05735839359788554 8 0.08738869922380073 9 0.0727559357014957 10-11 0.07270495046274203 12-13 0.07137933425514645 14-15 0.06464928273966121 16-17 0.0527697221100547 18-19 0.06421590821025497 20-21 0.05865851718610428 22-23 0.03466996235249971 24-25 0.010502959183257263 26-27 0.0033905183771194567 28-29 0.008157638200588166 30-31 0.0061692138891948 32-33 0.006398647463586343 34-35 0.00731638176115251 36-37 0.006526110560470533 38-39 0.003645444570887837 40-41 0.0076477858130514055 42-43 0.0037729076677720267 44-45 0.0022433505051617456 46-47 0.0016570202594944712 48-49 3.059114325220562E-4 50-51 0.0020649021695238793 52-53 0.00667906627673156 54-55 0.012185472062128572 56-57 0.008794953685009117 58-59 0.004971060778483413 60-61 0.0024727840795532876 62-63 0.00874396844625544 64-65 0.0023963062214227737 66-67 0.023070820536038407 68-69 0.04751824251842607 70-71 0.06059595625874397 72-73 0.0037474150483951885 74-75 0.015066138051711267 76-77 0.023376731968560462 78-79 0.025722052951229563 80-81 0.07561110907170156 82-83 0.028577226321435417 84-85 0.010885348473909833 86-87 0.03270703066048317 88-89 0.049328218494181564 90-91 0.04387279794753823 92-93 0.04254718173994265 94-95 0.0123384277783896 96-97 0.016774143549959417 98-99 0.03841737740089489 100-101 0.07426000024472915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1961352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.7461304217895 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74225639593764 43.738222162804355 2 13.716522913109852 15.84152242147811 3 4.657681892502921 8.068893180830429 4 2.05455808617069 4.745711168126196 5 1.0912361265800279 3.150733184322934 6 0.6562245051196937 2.2736655515169657 7 0.4214416281912665 1.7035636258692939 8 0.2811793703229613 1.2989616472469094 9 0.211002192153865 1.096610409666052 >10 1.07497844747345 11.089128482767784 >50 0.06405903656012427 2.5423531867479072 >100 0.026824200031594428 2.6563819102763087 >500 0.0011503337389498491 0.4832332194382778 >1k 7.963848961960425E-4 0.9916577233412922 >5k 8.848721068844916E-5 0.31936212556722193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6250 0.3186577422104752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4463 0.22754712055765616 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3591 0.18308799236445064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2858 0.14571581235800612 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2084 0.10625323756266085 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.098523875367604E-5 0.0 5 0.0 0.0 0.0 5.098523875367604E-5 0.0 6 0.0 0.0 0.0 5.098523875367604E-5 0.0 7 0.0 0.0 0.0 5.098523875367604E-5 0.0 8 0.0 0.0 0.0 5.098523875367604E-5 0.0 9 0.0 0.0 0.0 5.098523875367604E-5 0.0 10-11 0.0 0.0 0.0 5.098523875367604E-5 0.0 12-13 0.0 0.0 0.0 5.098523875367604E-5 0.0 14-15 0.0 0.0 0.0 5.098523875367604E-5 0.0 16-17 0.0 0.0 0.0 5.098523875367604E-5 0.0 18-19 0.0 0.0 0.0 1.274630968841901E-4 0.0 20-21 0.0 0.0 0.0 1.7844833563786613E-4 0.0 22-23 0.0 0.0 0.0 4.333745294062463E-4 0.0 24-25 0.0 0.0 0.0 7.392859619283025E-4 0.0 26-27 0.0 0.0 0.0 9.432269169430067E-4 0.0 28-29 0.0 0.0 0.0 0.0017844833563786613 0.0 30-31 0.0 0.0 0.0 0.002931651228336372 0.0 32-33 0.0 0.0 0.0 0.0040278338615404065 0.0 34-35 0.0 0.0 0.0 0.005837809837295906 0.0 36-37 0.0 0.0 0.0 0.007673278432428243 0.0 38-39 0.0 0.0 0.0 0.009967614176343665 0.0 40-41 0.0 0.0 0.0 0.013740521844115693 0.0 42-43 0.0 0.0 0.0 0.018482149048207566 0.0 44-45 0.0 0.0 0.0 0.02941848276087107 0.0 46-47 0.0 0.0 0.0 0.049685115165457296 0.0 48-49 0.0 0.0 0.0 0.06587292846974943 0.0 50-51 0.0 0.0 0.0 0.0850943634798853 0.0 52-53 0.0 0.0 0.0 0.10676308995019762 0.0 54-55 0.0 0.0 0.0 0.12983391048623602 0.0 56-57 0.0 0.0 0.0 0.15683059440630748 0.0 58-59 0.0 0.0 0.0 0.19665006587292846 0.0 60-61 0.0 0.0 0.0 0.24661559985153098 0.0 62-63 0.0 0.0 0.0 0.3156496131240083 0.0 64-65 0.0 0.0 0.0 0.3696174883447744 0.0 66-67 0.0 0.0 0.0 0.4499957172399447 0.0 68-69 0.0 0.0 0.0 0.5536742002455449 0.0 70-71 0.0 0.0 0.0 0.6191647394246418 0.0 72-73 0.0 0.0 0.0 0.7344678568660801 0.0 74-75 0.0 2.549261937683802E-5 0.0 0.8268786021071179 0.0 76-77 0.0 5.098523875367604E-5 0.0 0.9023367554625585 0.0 78-79 0.0 5.098523875367604E-5 0.0 0.9977301371706864 0.0 80-81 0.0 5.098523875367604E-5 0.0 1.09597869224902 0.0 82-83 0.0 5.098523875367604E-5 0.0 1.2302483185068258 0.0 84-85 0.0 5.098523875367604E-5 0.0 1.4312321296738169 0.0 86-87 0.0 5.098523875367604E-5 0.0 1.7132569778397757 0.0 88-89 0.0 5.098523875367604E-5 0.0 2.0469043802438316 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13280 0.0 26.346354 1 GTACATG 9910 0.0 23.697039 1 TACATGG 9870 0.0 23.217476 2 TATCAAC 15340 0.0 22.531712 2 TCAACGC 15385 0.0 22.497286 4 ATCAACG 15415 0.0 22.45293 3 CAACGCA 15525 0.0 22.263788 5 AACGCAG 15800 0.0 21.903019 6 ACATGGG 10175 0.0 21.727194 3 GAGTACT 5805 0.0 21.53278 12-13 GGTATCA 9755 0.0 20.32121 1 GTACTTT 6170 0.0 20.1809 14-15 ACGCAGA 17405 0.0 19.825575 7 CATGGGA 6080 0.0 19.783957 4 CGCAGAG 17330 0.0 19.609186 8 AGTACTT 5920 0.0 19.267975 12-13 AGAGTAC 12430 0.0 18.771849 10-11 GCAGAGT 17710 0.0 18.488316 9 CATGGGG 4445 0.0 17.969408 4 CGCGGAA 200 2.1827873E-11 17.815828 82-83 >>END_MODULE