FastQCFastQC Report
Thu 26 May 2016
SRR936888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961352
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.3658700732963793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.2864860565569056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38910.19838356399055346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.17931508469667862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141150.025.6838361
TACATGG92700.022.800932
GTACATG94550.022.6134721
ATCAACG163700.022.0224363
TCAACGC164550.021.9375444
GAGTACT69900.021.78186612-13
CAACGCA165550.021.7763395
GGTATCA103750.021.615741
TATCAAC167750.021.5473772
AACGCAG168200.021.4647736
AGTACTT73600.020.62230912-13
ACATGGG97050.020.6043453
GTACTTT74900.020.19889314-15
AGAGTAC135750.019.335910-11
ACGCAGA185600.019.3244747
CGCAGAG186100.019.0953318
AATTCGC1555.5933924E-918.3871378-79
GCAGAGT191800.018.007689
CATGGGA59750.017.7271614
CATGGGG39950.017.7150254
Produced by FastQC (version 0.11.5)