##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936888_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1961352 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14588712276022 33.0 31.0 34.0 31.0 34.0 2 32.27546355779075 33.0 31.0 34.0 31.0 34.0 3 32.40925392280427 34.0 31.0 34.0 31.0 34.0 4 36.068724023020856 37.0 35.0 37.0 35.0 37.0 5 35.91078551937643 37.0 35.0 37.0 35.0 37.0 6 35.99505290228373 37.0 35.0 37.0 35.0 37.0 7 35.94819593831194 37.0 35.0 37.0 35.0 37.0 8 35.8956138418805 37.0 35.0 37.0 35.0 37.0 9 37.602780632951145 39.0 37.0 39.0 35.0 39.0 10-11 37.51531316153347 39.0 37.0 39.0 35.0 39.0 12-13 37.42100653018938 39.0 37.0 39.0 34.0 39.0 14-15 38.62941226256174 40.0 38.0 41.0 34.0 41.0 16-17 38.62479503934021 40.0 38.0 41.0 34.5 41.0 18-19 38.74634767242188 40.0 38.0 41.0 34.5 41.0 20-21 38.74641981653472 40.0 38.0 41.0 34.0 41.0 22-23 38.65747045915266 40.0 38.0 41.0 34.0 41.0 24-25 38.569937216777 40.0 38.0 41.0 34.0 41.0 26-27 38.378107805228225 40.0 38.0 41.0 34.0 41.0 28-29 38.23343311144558 40.0 38.0 41.0 34.0 41.0 30-31 38.24100161521236 40.0 38.0 41.0 34.0 41.0 32-33 38.12993588096374 40.0 38.0 41.0 34.0 41.0 34-35 38.022885999045556 40.0 37.0 41.0 33.0 41.0 36-37 37.88811187385028 40.0 37.0 41.0 33.0 41.0 38-39 37.73249957172399 40.0 37.0 41.0 33.0 41.0 40-41 37.43242110544155 40.0 37.0 41.0 32.5 41.0 42-43 37.24228848263851 40.0 36.5 41.0 32.0 41.0 44-45 37.02998849773014 40.0 36.0 41.0 31.0 41.0 46-47 36.697558622827515 39.0 36.0 41.0 31.0 41.0 48-49 36.35805123200731 39.0 36.0 40.5 30.0 41.0 50-51 36.08846627224486 39.0 35.0 40.0 29.5 41.0 52-53 35.84124216356881 39.0 35.0 40.0 29.0 41.0 54-55 35.45561352577202 38.0 35.0 40.0 28.0 41.0 56-57 35.26666809425335 38.0 34.5 40.0 28.0 41.0 58-59 34.95492879401556 38.0 34.0 40.0 27.0 41.0 60-61 34.57978272130653 37.5 34.0 40.0 26.0 41.0 62-63 34.21265178305577 37.0 33.5 39.5 26.0 41.0 64-65 33.891030013990346 36.5 33.0 39.0 26.0 40.0 66-67 33.5562655250052 36.0 33.0 39.0 25.5 40.0 68-69 33.10693134123808 35.5 32.0 39.0 24.5 40.0 70-71 32.66703452516427 35.0 32.0 38.0 23.0 40.0 72-73 32.261004144080204 35.0 31.5 37.5 22.5 39.5 74-75 31.78114407816649 35.0 31.0 37.0 21.5 39.0 76-77 30.318125966170275 33.5 29.5 35.0 20.0 37.5 78-79 31.006647710354898 34.0 31.0 36.0 20.0 38.0 80-81 30.881618138916423 34.0 31.0 35.5 20.0 37.0 82-83 30.535148713744398 34.0 30.5 35.0 18.5 37.0 84-85 30.09826257601899 34.0 30.0 35.0 17.5 36.0 86-87 29.74294083876836 34.0 30.0 35.0 13.5 36.0 88-89 29.368973034926928 34.0 29.0 35.0 7.5 35.5 90-91 28.919101976595737 34.0 29.0 35.0 4.5 35.0 92-93 28.45580319086018 33.0 29.0 35.0 2.0 35.0 94-95 27.926930250153976 33.0 28.0 34.5 2.0 35.0 96-97 27.18092162956981 33.0 26.5 34.0 2.0 35.0 98-99 26.3453862437747 32.0 25.0 34.0 2.0 35.0 100-101 23.181642050993396 28.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1493311896324485 1101 2 0.30581221647000234 1101 3 0.2871590391486194 1101 4 0.07848462684668078 1101 5 0.04920548303142169 1101 6 0.11497597284019179 1101 7 0.13811809917746132 1101 8 0.22199328874734192 1101 9 0.21845493704107355 1101 10-11 0.2674694572621732 1101 12-13 0.3067875029198035 1101 14-15 0.4513779244861098 1101 16-17 0.5337595709101706 1101 18-19 0.3020849296175925 1101 20-21 0.20115177127954809 1101 22-23 0.33429106832166866 1101 24-25 0.13696797871613597 1101 26-27 0.272506617429336 1101 28-29 0.3663280252960206 1101 30-31 0.3513930059828496 1101 32-33 0.4043656637843256 1101 34-35 0.1818758297572174 1101 36-37 0.21903393356503642 1101 38-39 0.19984287015198277 1101 40-41 0.3080684397563971 1101 42-43 0.3230120724568408 1101 44-45 0.17866256072475295 1101 46-47 0.046055449815327165 1101 48-49 0.3764973208955311 1101 50-51 0.2497604339603967 1101 52-53 0.3335235491704793 1101 54-55 0.1570223303092746 1101 56-57 0.08797614344008053 1101 58-59 0.23184806504519173 1101 60-61 0.31430539176756866 1101 62-63 0.19395447835607627 1101 64-65 0.18800068211943 1101 66-67 0.3637926598796142 1101 68-69 -0.13870733191939166 1101 70-71 -0.036929489723078746 1101 72-73 0.21895675335590425 1101 74-75 0.13196891717464254 1101 76-77 0.13631612505167112 1101 78-79 0.16669281370099043 1101 80-81 0.15569274739928574 1101 82-83 0.3993120636570531 1101 84-85 0.3403307327560263 1101 86-87 0.007986258360265452 1101 88-89 0.1105183500985163 1101 90-91 0.2845561181737324 1101 92-93 0.18822740114940828 1101 94-95 0.04574705176207772 1101 96-97 0.05397686434364957 1101 98-99 0.42141848867576215 1101 100-101 0.7169388409277637 1102 1 -0.04075178973086224 1102 2 0.06198439369372011 1102 3 0.10880985364592277 1102 4 0.14276885766530256 1102 5 0.003400081660011267 1102 6 0.1092323083219 1102 7 0.17256802157551476 1102 8 0.21696488927783975 1102 9 0.2903683744583958 1102 10-11 0.3479884811580547 1102 12-13 0.3362331552716853 1102 14-15 0.34085051561561386 1102 16-17 0.369469652441758 1102 18-19 0.40206045548821123 1102 20-21 0.36698511107611864 1102 22-23 0.19507808393426984 1102 24-25 0.23344775438143728 1102 26-27 0.2955476314166532 1102 28-29 0.1224851216301559 1102 30-31 0.1871698743111665 1102 32-33 4.48596540607582E-5 1102 34-35 0.21550793705731763 1102 36-37 0.09663743371024935 1102 38-39 0.24904578050514203 1102 40-41 0.3730093355957109 1102 42-43 0.28476262764901605 1102 44-45 0.26288329668939525 1102 46-47 0.06672402442997338 1102 48-49 0.12705208219090736 1102 50-51 0.03792003975456737 1102 52-53 0.10153120853885866 1102 54-55 0.413040251576696 1102 56-57 0.4014074422321485 1102 58-59 0.17106044619701066 1102 60-61 0.26406190003319097 1102 62-63 0.20185858802775414 1102 64-65 0.23200542527464307 1102 66-67 0.13680717458951364 1102 68-69 0.2261261018095837 1102 70-71 0.13932905360086068 1102 72-73 0.12295999703981408 1102 74-75 0.19336709660965212 1102 76-77 0.24321593365363725 1102 78-79 -0.16751133328511258 1102 80-81 0.07441149225562427 1102 82-83 0.2709792947798739 1102 84-85 0.20159602800968202 1102 86-87 0.2598849423587488 1102 88-89 0.3333191039706449 1102 90-91 0.34516750605507696 1102 92-93 0.31282484911818287 1102 94-95 0.2283912746179837 1102 96-97 0.51012637714474 1102 98-99 0.6034719715425787 1102 100-101 0.6662670519292284 1103 1 0.20382262984523436 1103 2 0.2433881888896181 1103 3 0.2419932647304961 1103 4 0.12479785149090361 1103 5 0.11727376220073182 1103 6 0.1405270498295863 1103 7 0.16769222692382613 1103 8 0.16867993390376057 1103 9 0.15919836443523394 1103 10-11 0.26672381976124626 1103 12-13 0.30536556728205255 1103 14-15 0.3406298307031861 1103 16-17 0.3866489299026128 1103 18-19 0.3344298966465189 1103 20-21 0.26789439668942805 1103 22-23 0.29721680107599724 1103 24-25 0.2889520692298504 1103 26-27 0.3514141299323086 1103 28-29 0.28908588840725713 1103 30-31 0.35644848841523213 1103 32-33 0.32152591184145507 1103 34-35 0.41010791941315006 1103 36-37 0.5159563052065224 1103 38-39 0.3995645827002505 1103 40-41 0.4390770307258123 1103 42-43 0.23327435304630484 1103 44-45 0.3333177895419155 1103 46-47 0.5144744466675704 1103 48-49 0.3538403075812866 1103 50-51 0.2084704154370769 1103 52-53 0.21316190130613677 1103 54-55 0.44667829218165167 1103 56-57 0.19315342791912116 1103 58-59 0.1394597219359639 1103 60-61 0.06631254069703374 1103 62-63 0.3260936975756934 1103 64-65 0.43141809250889906 1103 66-67 0.32702720581756495 1103 68-69 0.2538966607150499 1103 70-71 0.20803991889830087 1103 72-73 0.45570076281779137 1103 74-75 0.33363992746695104 1103 76-77 0.41506583921484363 1103 78-79 0.3440149028426944 1103 80-81 0.42215776917758774 1103 82-83 0.6035871978772747 1103 84-85 0.43317395303473916 1103 86-87 0.2741227729343869 1103 88-89 0.5415351195570288 1103 90-91 0.44133816444811913 1103 92-93 0.40908572127133525 1103 94-95 0.38834040415484594 1103 96-97 0.48581011507150507 1103 98-99 0.4988642672077468 1103 100-101 0.9816629933463545 1104 1 0.07733757108276507 1104 2 0.1533603895849609 1104 3 0.05853029610602789 1104 4 0.059377058179798325 1104 5 -0.0840490154546032 1104 6 0.09607482410776669 1104 7 0.16255717633762146 1104 8 0.06896859164525893 1104 9 0.1811072026233731 1104 10-11 0.12690256004749045 1104 12-13 0.18602141046837062 1104 14-15 0.24990438260824988 1104 16-17 0.14236431763537638 1104 18-19 0.22666493230214968 1104 20-21 0.24677030229976538 1104 22-23 0.24506111075030645 1104 24-25 0.18473044976038722 1104 26-27 0.14224658046590122 1104 28-29 0.14174601880092297 1104 30-31 0.05441277590696103 1104 32-33 0.20500078937521238 1104 34-35 0.15446274092170853 1104 36-37 0.20112384756313162 1104 38-39 0.14889626182090865 1104 40-41 0.26825972405895726 1104 42-43 0.06609281860999516 1104 44-45 0.15361945821117473 1104 46-47 0.1944731669041886 1104 48-49 0.3439905731321815 1104 50-51 0.2605203475385167 1104 52-53 0.25220534927309046 1104 54-55 0.40442032743202816 1104 56-57 0.3709534435606727 1104 58-59 0.17490827900275008 1104 60-61 0.2266508959166984 1104 62-63 0.3312838490854588 1104 64-65 0.21347172170457185 1104 66-67 0.08582735654524498 1104 68-69 0.13705515230060428 1104 70-71 0.1873619642445874 1104 72-73 0.2318523721202439 1104 74-75 0.07353601778980945 1104 76-77 0.318372605608257 1104 78-79 0.44058104883525573 1104 80-81 0.310366882514856 1104 82-83 0.3758426386491145 1104 84-85 0.3049976514087298 1104 86-87 0.388208346446131 1104 88-89 0.42355870986211386 1104 90-91 0.310054881712837 1104 92-93 0.30123188446960825 1104 94-95 0.3245050954830262 1104 96-97 0.5888612842775558 1104 98-99 0.3302474425850974 1104 100-101 0.7850780063928333 1105 1 0.03470319346293138 1105 2 0.11063592308239123 1105 3 0.03601254263983833 1105 4 0.08585524798392186 1105 5 0.0041075476073686445 1105 6 0.11929889426888707 1105 7 0.10356360069248893 1105 8 0.05801100390291225 1105 9 0.08963348251836578 1105 10-11 0.11862381362809771 1105 12-13 0.10950629166769232 1105 14-15 0.21304500018615613 1105 16-17 -0.053358123232321475 1105 18-19 -0.1242236964954202 1105 20-21 0.0579600116179364 1105 22-23 0.018507673152122095 1105 24-25 -0.055035899882760475 1105 26-27 0.08997306466162769 1105 28-29 0.07139330221161799 1105 30-31 0.14165753027702266 1105 32-33 0.1933104539020789 1105 34-35 0.11504699338500757 1105 36-37 0.07568537777368789 1105 38-39 -0.09949417495878521 1105 40-41 -0.09861099516799499 1105 42-43 0.08466390965833881 1105 44-45 0.04888108079385489 1105 46-47 -2.0903277348338634E-4 1105 48-49 0.02307523533858813 1105 50-51 -0.06826101542934282 1105 52-53 0.2196675032560833 1105 54-55 0.27715302850520374 1105 56-57 0.14341299911225036 1105 58-59 -0.0032418661569977303 1105 60-61 -0.004448307578456934 1105 62-63 0.11451498241353164 1105 64-65 0.22258124645716748 1105 66-67 0.1008070530425016 1105 68-69 0.008728750599487967 1105 70-71 -0.08457145237753139 1105 72-73 -0.10217896392021686 1105 74-75 -0.2508362016776289 1105 76-77 -0.009729243968621404 1105 78-79 0.1571426862590215 1105 80-81 -0.022001780054875297 1105 82-83 0.07285610532391829 1105 84-85 -0.1056872871695127 1105 86-87 -0.020873853827165334 1105 88-89 0.16502285026774643 1105 90-91 0.10163598875877966 1105 92-93 0.10383138304927897 1105 94-95 0.038821956200649765 1105 96-97 0.24587888561017834 1105 98-99 0.3871475064663059 1105 100-101 0.8572777932569302 1106 1 0.07981435253912395 1106 2 0.14097083926341014 1106 3 0.09464985467668185 1106 4 0.12225924587345816 1106 5 -0.009498047519379327 1106 6 0.11191227173009821 1106 7 0.10182036976461006 1106 8 0.10584867807611431 1106 9 0.22649134999084453 1106 10-11 0.22696494128715727 1106 12-13 0.24490422197442996 1106 14-15 0.3357269377356289 1106 16-17 0.25972120501672435 1106 18-19 0.1408216005283407 1106 20-21 0.15048465367851804 1106 22-23 0.08613169286576294 1106 24-25 0.21082630243395073 1106 26-27 0.39164944425728976 1106 28-29 0.3255909962903232 1106 30-31 0.19659403650049256 1106 32-33 0.17977755585186372 1106 34-35 0.17802589474481323 1106 36-37 0.1699442573093819 1106 38-39 0.25380537297031935 1106 40-41 0.3201541438108251 1106 42-43 0.4273631745934736 1106 44-45 0.22696268738425118 1106 46-47 0.2238276185443624 1106 48-49 0.2724127176514628 1106 50-51 0.2628561812514789 1106 52-53 0.2121384843818248 1106 54-55 0.5607345811348878 1106 56-57 0.23085933817741733 1106 58-59 0.2047886875998728 1106 60-61 0.3357380367628693 1106 62-63 0.14382989115996025 1106 64-65 0.2370194854192036 1106 66-67 0.23386968344756554 1106 68-69 0.3104253654638711 1106 70-71 0.35755642852517155 1106 72-73 0.32991147424079514 1106 74-75 0.13780536726018155 1106 76-77 0.25431628088928093 1106 78-79 0.22285859507740113 1106 80-81 0.24397433933021162 1106 82-83 0.3723343351725923 1106 84-85 0.30092619821312994 1106 86-87 0.23576406376882275 1106 88-89 0.35859193197626027 1106 90-91 0.309700267963521 1106 92-93 0.5395177034080589 1106 94-95 0.6900858360960882 1106 96-97 0.6846259777271122 1106 98-99 0.6569218607363965 1106 100-101 1.0426605845848407 1107 1 0.24861610749796625 1107 2 0.27480843021398016 1107 3 0.2934684391550064 1107 4 0.2325266133514603 1107 5 0.12354568829903201 1107 6 0.15812646713672507 1107 7 0.173066905479196 1107 8 0.257300224131221 1107 9 0.3515232716540382 1107 10-11 0.281263167070648 1107 12-13 0.26385051403776316 1107 14-15 0.3741043648286393 1107 16-17 0.3518777383868894 1107 18-19 0.36810724394348426 1107 20-21 0.3639327794059142 1107 22-23 0.28943841085827415 1107 24-25 0.3026861213282288 1107 26-27 0.40201718181725 1107 28-29 0.41667946220845664 1107 30-31 0.3574190388030374 1107 32-33 0.35797814739691347 1107 34-35 0.34298841557239257 1107 36-37 0.35453048975769974 1107 38-39 0.33833158222572024 1107 40-41 0.40800465413879294 1107 42-43 0.4113246320633479 1107 44-45 0.25507075843663785 1107 46-47 0.25848381699871936 1107 48-49 0.2785448168200375 1107 50-51 0.12266801089654678 1107 52-53 0.27612153345536683 1107 54-55 0.45404924291177906 1107 56-57 0.3127064826014916 1107 58-59 0.28943656548956653 1107 60-61 0.2956779193748744 1107 62-63 0.2449150707167007 1107 64-65 0.40272300747759715 1107 66-67 0.42596221649957045 1107 68-69 0.1919355531841802 1107 70-71 0.16590373412746828 1107 72-73 0.331718973005799 1107 74-75 0.46382759311066124 1107 76-77 0.12234349070566353 1107 78-79 0.38706232227172777 1107 80-81 0.3703660715147805 1107 82-83 0.5683179096453053 1107 84-85 0.5724858802105643 1107 86-87 0.7777392192953521 1107 88-89 0.7337729958363326 1107 90-91 0.7080499179764956 1107 92-93 0.7463088540950551 1107 94-95 0.8174961833200527 1107 96-97 0.8884981278028903 1107 98-99 0.9212287838648479 1107 100-101 1.4198707672814201 1108 1 -0.011318862270876195 1108 2 0.10048359601555745 1108 3 0.07653050244933013 1108 4 0.050370822427531436 1108 5 -0.08227249102132106 1108 6 0.027183577849541507 1108 7 0.09855395757452357 1108 8 0.09746416861167262 1108 9 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-1.5690350988632602 2316 96-97 -1.1478630779326728 2316 98-99 -1.5261183206269529 2316 100-101 -2.326269215290079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 16.0 5 49.0 6 72.0 7 189.0 8 458.0 9 885.0 10 1587.0 11 2708.0 12 4040.0 13 5634.0 14 7183.0 15 9814.0 16 13219.0 17 17778.0 18 15602.0 19 10000.0 20 8972.0 21 9238.0 22 9994.0 23 11144.0 24 13033.0 25 15741.0 26 19377.0 27 24439.0 28 30936.0 29 38570.0 30 48338.0 31 61468.0 32 78505.0 33 103522.0 34 141543.0 35 200618.0 36 308245.0 37 450083.0 38 283578.0 39 14768.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93028737417085 19.617592596842357 15.106535644729213 22.34558438425758 2 15.410038173796572 21.074107744227916 39.80746374967942 23.70839033229609 3 19.416634715786728 25.318200172942635 30.197252496247472 25.067912615023168 4 12.911909743890948 17.35144940836729 37.91231762580098 31.82432322194078 5 13.603361440512476 37.859340376953675 34.10438576007163 14.432912422462222 6 30.981384269626254 36.96965154648426 17.700596323352464 14.348367860537017 7 27.81760874176075 33.3183776022972 21.101397817007115 17.762615838934934 8 26.35880759802422 34.48284652627371 19.926662832576714 19.231683043125354 9 26.47940118826258 16.793169606052157 20.672995297629033 36.05443390805623 10-11 24.602493586056966 26.949904963514964 27.542047526400154 20.905553924027913 12-13 25.871472344856087 24.408465989933386 28.027154311657114 21.692907353553412 14-15 22.947226355694706 25.531594870012945 26.733685431257925 24.787493343034424 16-17 21.912028029644855 28.785246095550416 27.18678238276454 22.115943492040184 18-19 21.792766030019727 27.45872918231814 29.60185199583857 21.146652791823563 20-21 22.917855272771398 26.20348120740456 29.606935290371688 21.271728229452346 22-23 22.42122343469776 26.35813335134839 29.353790618320037 21.866852595633812 24-25 22.238701868414584 26.473863303799135 29.519686010904778 21.7677488168815 26-27 22.27972802378772 26.52109671511634 29.566221802556203 21.63295345853974 28-29 21.865291287814188 26.822538857792118 29.62002456980535 21.69214528458835 30-31 22.18194388360682 26.489508257569266 29.501155325510158 21.827392533313756 32-33 22.15235259972524 26.55719793214016 29.360774955432518 21.929674512702082 34-35 22.451732274471894 26.50804139185623 29.330584209259737 21.70964212441214 36-37 22.443663506800913 26.321847431637153 29.40722244103772 21.827266620524213 38-39 22.21478330659743 26.681073121084587 29.3591082411514 21.745035331166587 40-41 22.411877189033312 26.58155306615708 29.1736846170691 21.832885127740507 42-43 22.13251369463513 26.731586171171724 28.956658468240278 22.179241665952873 44-45 22.401420260017304 26.615165925588286 28.809976490454698 22.173437323939712 46-47 22.56185044540886 26.932399415910723 28.340566468707173 22.165183669973242 48-49 22.59266123384819 26.957483143638456 28.219368124479473 22.23048749803388 50-51 22.486216563679132 27.10906906389391 28.206823817512834 22.197890554914125 52-53 22.67449463823886 27.34965973561476 27.77541403400987 22.200431592136507 54-55 22.4337854704306 27.02204907635141 28.026585742895715 22.517579710322266 56-57 22.569022133020418 27.271151360124975 27.797907099998444 22.36191940685616 58-59 22.839186570681782 27.055669040286272 27.853206247430023 22.251938141601922 60-61 22.621580543238125 27.13756039958946 28.241922630401167 21.998936426771248 62-63 22.497655952453183 27.05081854293291 28.233778655630736 22.217746848983175 64-65 22.428109428765612 27.148075125844507 28.080724032285943 22.34309141310394 66-67 22.45786767841852 27.19004073978618 27.77802845539336 22.574063126401935 68-69 22.452392484768144 27.116322940831573 27.989700971269787 22.441583603130496 70-71 22.58513927942305 27.003153448271465 27.91714359278964 22.494563679515842 72-73 22.52121435838659 26.94778617874386 27.93478568444109 22.596213778428464 74-75 22.414078803734046 27.482189136507717 27.57564741398958 22.528084645768654 76-77 22.50250528908525 27.123097070105523 27.71139069737918 22.66300694343005 78-79 22.47293698007697 27.17536612574617 27.73773445609856 22.6139624380783 80-81 22.498382467734757 27.433270739465897 27.451243320190834 22.617103472608513 82-83 22.536322530663167 27.320115746718525 27.625466419456174 22.51809530316213 84-85 22.54308720739627 27.218399103049844 27.43251587074577 22.805997818808116 86-87 22.37229761681701 27.19273356710119 27.5406576346109 22.894311181470904 88-89 22.458619078319938 27.071888556714725 27.461982883470597 23.007509481494736 90-91 22.386390867421596 26.927238149622962 27.71114498834974 22.9752259946057 92-93 22.485841399198105 26.938688210989152 27.68182355844336 22.89364683136938 94-95 22.23505067806561 26.962454205684196 27.736180893633804 23.066314222616395 96-97 22.028466639151585 26.922293513875783 27.813389614339318 23.23585023263331 98-99 22.07279804052731 26.88358085068198 27.538488913724468 23.50513219506624 100-101 21.977097502720753 26.95483054859381 27.538928611398124 23.529143337287316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 287.5 2 219.5 3 243.0 4 206.0 5 200.5 6 367.5 7 753.0 8 1243.5 9 1546.0 10 1502.5 11 1393.0 12 1490.0 13 1691.5 14 1887.0 15 2062.5 16 2244.0 17 2446.0 18 2684.0 19 2974.0 20 3306.0 21 3733.5 22 4331.5 23 5191.5 24 6257.5 25 7805.5 26 10220.0 27 13230.5 28 15771.5 29 18876.5 30 22689.0 31 26298.0 32 29960.5 33 33825.0 34 38929.0 35 43659.5 36 48559.5 37 53905.5 38 57162.0 39 58625.0 40 60266.0 41 62774.5 42 64326.0 43 66515.5 44 69765.5 45 71761.5 46 72954.0 47 76526.0 48 85328.0 49 84611.5 50 74747.0 51 69508.5 52 66630.0 53 64757.0 54 62573.5 55 58833.5 56 53850.5 57 48265.5 58 43630.0 59 39078.0 60 33105.5 61 26757.0 62 20817.5 63 15738.0 64 11729.5 65 8611.5 66 6071.5 67 4512.0 68 3230.0 69 2043.5 70 1330.5 71 898.5 72 613.5 73 446.0 74 298.0 75 204.0 76 146.5 77 63.5 78 23.0 79 12.5 80 8.0 81 6.5 82 4.0 83 1.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0234022245879373 2 0.003008129086466886 3 4.078819100294083E-4 4 0.0 5 0.002549261937683802 6 0.0 7 4.078819100294083E-4 8 0.0 9 5.098523875367604E-5 10-11 0.0 12-13 0.0059397803148032585 14-15 0.0030846069445974 16-17 0.0 18-19 4.3337452940624626E-4 20-21 0.0010706900138271968 22-23 0.001504064543233443 24-25 5.098523875367604E-4 26-27 2.0394095501470415E-4 28-29 7.647785813051406E-5 30-31 0.0 32-33 2.549261937683802E-5 34-35 0.0 36-37 0.0017589907370018233 38-39 0.00989113631821315 40-41 6.373154844209505E-4 42-43 0.0 44-45 0.0010706900138271968 46-47 4.078819100294083E-4 48-49 2.549261937683802E-5 50-51 0.0015550497819871191 52-53 4.8435976815992235E-4 54-55 0.0 56-57 3.3140405189889424E-4 58-59 7.647785813051406E-5 60-61 0.0019884243113933654 62-63 5.098523875367604E-5 64-65 1.529557162610281E-4 66-67 7.647785813051406E-5 68-69 1.0197047750735207E-4 70-71 3.5689667127573226E-4 72-73 1.7844833563786613E-4 74-75 0.002498276698930126 76-77 7.647785813051406E-5 78-79 2.0394095501470415E-4 80-81 0.001580542401363957 82-83 2.549261937683802E-5 84-85 0.0019884243113933654 86-87 2.2943357439154214E-4 88-89 0.001606035020740795 90-91 0.0011216752525808728 92-93 0.0 94-95 2.549261937683802E-5 96-97 1.7844833563786613E-4 98-99 4.078819100294083E-4 100-101 0.0019119464532628514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1961352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.22173631069723 #Duplication Level Percentage of deduplicated Percentage of total 1 74.65122165886315 41.97021299376014 2 14.25920359056739 16.03354368538854 3 4.796267192108828 8.089634080511722 4 2.191133742634141 4.927573739993912 5 1.1685914863152351 3.2850121199270452 6 0.69127492160619 2.331880581644467 7 0.4458339727988896 1.7545892039934814 8 0.3261453173960601 1.4669164826887955 9 0.2358409125965749 1.1933447039350944 >10 1.1365097934376265 11.418051246025502 >50 0.0647836489688402 2.5101651239524556 >100 0.03091497093301968 2.9667340960799904 >500 0.001093820051520824 0.4093614520946334 >1k 0.0010026683805607502 0.987274458513155 >5k 1.823033419201364E-4 0.6557060314910884 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7176 0.3658700732963793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5619 0.2864860565569056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3891 0.19838356399055346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.17931508469667862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.647785813051406E-5 0.0 20-21 0.0 0.0 0.0 1.274630968841901E-4 0.0 22-23 0.0 0.0 0.0 3.3140405189889424E-4 0.0 24-25 0.0 0.0 0.0 6.373154844209505E-4 0.0 26-27 0.0 0.0 0.0 8.412564394356546E-4 0.0 28-29 0.0 0.0 0.0 0.0016825128788713092 0.0 30-31 0.0 0.0 0.0 0.00282968075082902 0.0 32-33 0.0 0.0 0.0 0.004002341242163568 0.0 34-35 0.0 0.0 0.0 0.00578682459854223 0.0 36-37 0.0 0.0 0.0 0.007571307954920892 0.0 38-39 0.0 0.0 0.0 0.009814658460082637 0.0 40-41 0.0 0.0 0.0 0.01351108826972415 0.0 42-43 0.0 0.0 0.0 0.018201730235062344 0.0 44-45 0.0 0.0 0.0 0.029265527044610044 0.0 46-47 0.0 0.0 0.0 0.049736100404210976 0.0 48-49 0.0 0.0 0.0 0.06546504655972003 0.0 50-51 0.0 0.0 0.0 0.08410015132418863 0.0 52-53 0.0 0.0 0.0 0.10546296636197888 0.0 54-55 0.0 0.0 0.0 0.12845730903988678 0.0 56-57 0.0 0.0 0.0 0.1553520224824509 0.0 58-59 0.0 0.0 0.0 0.195146001329695 0.0 60-61 0.0 0.0 0.0 0.24500956483079017 0.0 62-63 0.0 0.0 0.0 0.3138651297676297 0.0 64-65 0.0 0.0 0.0 0.36770554189151156 0.0 66-67 0.0 0.0 0.0 0.44795630768979766 0.0 68-69 0.0 0.0 0.0 0.5520426726054273 0.0 70-71 0.0 0.0 0.0 0.6177371527395389 0.0 72-73 0.0 0.0 0.0 0.7336011078072676 0.0 74-75 0.0 0.0 0.0 0.8262922718614507 0.0 76-77 0.0 0.0 0.0 0.9014954990231228 0.0 78-79 0.0 0.0 0.0 0.9973222552606569 0.0 80-81 0.0 0.0 0.0 1.0968964265465861 0.0 82-83 0.0 0.0 0.0 1.232160264960089 0.0 84-85 0.0 0.0 0.0 1.4334244949402248 0.0 86-87 0.0 0.0 0.0 1.7162905995456197 0.0 88-89 0.0 0.0 0.0 2.0493771643233853 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14115 0.0 25.683836 1 TACATGG 9270 0.0 22.80093 2 GTACATG 9455 0.0 22.613472 1 ATCAACG 16370 0.0 22.022436 3 TCAACGC 16455 0.0 21.937544 4 GAGTACT 6990 0.0 21.781866 12-13 CAACGCA 16555 0.0 21.776339 5 GGTATCA 10375 0.0 21.61574 1 TATCAAC 16775 0.0 21.547377 2 AACGCAG 16820 0.0 21.464773 6 AGTACTT 7360 0.0 20.622309 12-13 ACATGGG 9705 0.0 20.604345 3 GTACTTT 7490 0.0 20.198893 14-15 AGAGTAC 13575 0.0 19.3359 10-11 ACGCAGA 18560 0.0 19.324474 7 CGCAGAG 18610 0.0 19.095331 8 AATTCGC 155 5.5933924E-9 18.38713 78-79 GCAGAGT 19180 0.0 18.00768 9 CATGGGA 5975 0.0 17.727161 4 CATGGGG 3995 0.0 17.715025 4 >>END_MODULE