FastQCFastQC Report
Thu 26 May 2016
SRR936887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322712
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70220.530878981970376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63210.47788180647034273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.395021743206382No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39540.2989312866292889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.25803047072983387No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29950.22642873127332327No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23180.17524600971337675No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21110.15959634448012872No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17840.134874409546447No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17600.13305995560636028No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16260.12292925444087602No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13450.10168502289236055No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13280.1003997846847991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG77550.024.2072681
TACATGG78350.023.4141712
ACATGGG77900.022.8173223
GAGTACT52900.022.09462512-13
CATGGGA52950.020.4644174
GTACTTT57800.020.26226814-15
AGTACTT57950.019.8002612-13
TTTGCGT2001.2789418E-519.0103663
GTATCAA161550.018.2984331
AGAGTAC109200.018.125510-11
CATGGGG26750.017.4113644
ACTGCGT1650.001458260617.2828068
GACCGAC1650.001458630417.2821527
ACTTTTT69600.017.2720216-17
ATGGGAG25350.017.0605855
TACTTTT68350.016.71766314-15
ATGGGAT21350.016.4726835
CAACGCA180400.016.2810525
TCAACGC180400.016.2810524
TATCAAC180800.016.2450312
Produced by FastQC (version 0.11.5)