##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936887_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1322712 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59020784569884 31.0 31.0 34.0 30.0 34.0 2 31.665800264910274 33.0 31.0 34.0 30.0 34.0 3 31.66501778164861 33.0 31.0 34.0 30.0 34.0 4 35.24795722727245 37.0 35.0 37.0 33.0 37.0 5 35.290867550910555 37.0 35.0 37.0 33.0 37.0 6 35.3540551533516 37.0 35.0 37.0 33.0 37.0 7 35.29682652005879 37.0 35.0 37.0 33.0 37.0 8 35.26739683317306 37.0 35.0 37.0 32.0 37.0 9 36.843397504521015 39.0 37.0 39.0 33.0 39.0 10-11 36.70571522750228 39.0 37.0 39.0 32.0 39.0 12-13 36.58209534653046 39.0 37.0 39.0 32.0 39.0 14-15 37.6454209230732 40.0 37.0 41.0 32.0 41.0 16-17 37.593082621160164 40.0 37.0 41.0 32.0 41.0 18-19 37.58695883911237 40.0 37.0 41.0 32.0 41.0 20-21 37.53557123546169 40.0 37.0 41.0 32.0 41.0 22-23 37.379907341885456 40.0 37.0 41.0 32.0 41.0 24-25 37.197781527649255 39.5 36.5 41.0 31.0 41.0 26-27 37.151679277121545 39.0 36.5 41.0 31.0 41.0 28-29 37.232787258299616 40.0 37.0 41.0 31.0 41.0 30-31 37.09520704431502 39.0 36.5 41.0 31.0 41.0 32-33 36.89325491868222 39.0 36.0 41.0 30.5 41.0 34-35 36.6867050423675 39.0 35.5 41.0 30.0 41.0 36-37 36.518885441426406 39.0 35.0 41.0 30.0 41.0 38-39 36.35231252154664 39.0 35.0 41.0 30.0 41.0 40-41 36.15478879756138 39.0 35.0 41.0 30.0 41.0 42-43 35.92491827397045 39.0 35.0 40.0 29.0 41.0 44-45 35.634012543924904 39.0 35.0 40.0 27.5 41.0 46-47 35.24186746623604 38.0 35.0 40.0 26.0 41.0 48-49 34.86274336363472 38.0 34.0 40.0 24.0 41.0 50-51 34.19365629101422 37.5 33.5 39.5 24.0 40.5 52-53 34.496397174895215 38.0 34.0 40.0 24.0 40.5 54-55 34.81205923889705 38.0 34.0 40.0 24.0 41.0 56-57 34.681204978861615 38.0 34.0 40.0 23.5 41.0 58-59 34.426172893267776 38.0 34.0 40.0 23.0 41.0 60-61 34.11177830094533 38.0 34.0 40.0 22.5 41.0 62-63 33.80710918174175 37.5 33.0 40.0 22.0 41.0 64-65 33.50621034662119 37.0 33.0 40.0 21.5 41.0 66-67 33.10491701897314 36.5 32.5 39.0 20.0 41.0 68-69 32.655370556855914 36.0 32.0 39.0 18.0 41.0 70-71 32.27973701002183 35.5 32.0 39.0 18.0 40.0 72-73 31.890069417983657 35.0 31.0 38.0 15.5 40.0 74-75 31.46694102722286 35.0 31.0 37.0 12.0 39.5 76-77 31.029865155831352 35.0 31.0 37.0 9.0 39.0 78-79 30.59271141412492 35.0 30.5 36.0 7.0 39.0 80-81 30.152028937516256 34.5 30.0 36.0 4.5 37.5 82-83 29.73467920454339 34.0 29.5 35.0 2.0 37.0 84-85 29.372737602743456 34.0 29.0 35.0 2.0 37.0 86-87 29.060767196487216 34.0 29.0 35.0 2.0 36.0 88-89 28.67381826126927 34.0 29.0 35.0 2.0 36.0 90-91 28.31870732253128 34.0 29.0 35.0 2.0 35.5 92-93 27.950347467929525 34.0 27.5 35.0 2.0 35.0 94-95 27.53177108849092 34.0 27.0 35.0 2.0 35.0 96-97 27.080372371309856 34.0 26.0 35.0 2.0 35.0 98-99 26.46960562843612 33.0 25.0 35.0 2.0 35.0 100-101 24.278779507557203 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6682812998427394 1101 2 0.6182146346468294 1101 3 0.7292877279644294 1101 4 0.43164926662212366 1101 5 0.39655383197013094 1101 6 0.3383055352890949 1101 7 0.4586914745816273 1101 8 0.4493432609172956 1101 9 0.6817856882184969 1101 10-11 0.6475385717091413 1101 12-13 0.846195565023649 1101 14-15 1.0363225009714796 1101 16-17 1.05827162863973 1101 18-19 1.0750483432362117 1101 20-21 0.9300205759222706 1101 22-23 1.0074132147439556 1101 24-25 0.9866599262700575 1101 26-27 0.9243161097710768 1101 28-29 0.9982642649632965 1101 30-31 1.0144844275117322 1101 32-33 0.981143143838608 1101 34-35 1.0493684103680465 1101 36-37 1.0943246379547489 1101 38-39 1.0843081955047111 1101 40-41 1.3208831035321111 1101 42-43 1.4360167111526962 1101 44-45 1.279035853535639 1101 46-47 1.1327624061722545 1101 48-49 1.2941969363312964 1101 50-51 1.1997683995444248 1101 52-53 1.46520904682081 1101 54-55 1.536942402876221 1101 56-57 1.3529374364360436 1101 58-59 1.4978431022174519 1101 60-61 1.5249553247786238 1101 62-63 1.654370412798876 1101 64-65 1.4158126434601357 1101 66-67 1.3189178977223222 1101 68-69 1.3589741043602004 1101 70-71 1.5118536080541674 1101 72-73 1.6216024640543516 1101 74-75 1.4418675029049837 1101 76-77 1.3657570979445417 1101 78-79 1.603040050347449 1101 80-81 1.6219121303837554 1101 82-83 1.37919247727476 1101 84-85 1.570491880600276 1101 86-87 1.6283612304144945 1101 88-89 1.753142346512238 1101 90-91 1.5178233933559113 1101 92-93 1.3647048903113053 1101 94-95 1.4108735597243864 1101 96-97 1.4707770184319955 1101 98-99 1.5758190415608837 1101 100-101 2.083461952986223 1102 1 0.3673863690351986 1102 2 0.5318142042169107 1102 3 0.4887935285470455 1102 4 0.3407344326897075 1102 5 0.365733800511272 1102 6 0.29645005866461815 1102 7 0.384095925385985 1102 8 0.5241382672488157 1102 9 0.5555101562854787 1102 10-11 0.5866895941221699 1102 12-13 0.42145301378942435 1102 14-15 0.6696422059168796 1102 16-17 0.48170599659484736 1102 18-19 0.47335714547126884 1102 20-21 0.45990472042861086 1102 22-23 0.6281078508376012 1102 24-25 0.6259118839312023 1102 26-27 0.6054029595124248 1102 28-29 0.6309440745065729 1102 30-31 0.6118113469849931 1102 32-33 0.5999887200070972 1102 34-35 0.2863185191112194 1102 36-37 0.3600757182884209 1102 38-39 0.34584015407440916 1102 40-41 0.42565772406668145 1102 42-43 0.35434824156387634 1102 44-45 0.3389379420860905 1102 46-47 0.24753685635197797 1102 48-49 0.19624005882210582 1102 50-51 0.15654014676054118 1102 52-53 0.33404787977652717 1102 54-55 0.35371972921365114 1102 56-57 0.37850095608079215 1102 58-59 0.2691314249603991 1102 60-61 0.44412159395064776 1102 62-63 0.5388357541048165 1102 64-65 0.6345681445021327 1102 66-67 0.4146580327569396 1102 68-69 0.3630807209425342 1102 70-71 0.6395132731365365 1102 72-73 0.5409291507093208 1102 74-75 0.5760045562203757 1102 76-77 0.6525622631035723 1102 78-79 0.569888286890837 1102 80-81 0.6423884283457468 1102 82-83 0.7575530815801947 1102 84-85 0.8510815781068466 1102 86-87 0.9505359662225175 1102 88-89 0.7335888039831495 1102 90-91 0.5479119636812335 1102 92-93 0.6042209689698161 1102 94-95 0.6849911925869776 1102 96-97 0.5802378169419562 1102 98-99 0.6947867053712642 1102 100-101 1.1398195959565314 1103 1 0.43501527244841753 1103 2 0.6396573414718141 1103 3 0.571627588096792 1103 4 0.4641458342873861 1103 5 0.3704995957182433 1103 6 0.4079242779819765 1103 7 0.40865111783333674 1103 8 0.38361539143182455 1103 9 0.46682097691002866 1103 10-11 0.598082114093117 1103 12-13 0.37797080609153255 1103 14-15 0.5190203831136841 1103 16-17 0.6120845368998573 1103 18-19 0.5555557656600882 1103 20-21 0.37997552652882405 1103 22-23 0.5069204870031783 1103 24-25 0.7837958708593078 1103 26-27 0.9080309551551267 1103 28-29 0.8582498116162327 1103 30-31 1.0363756171375016 1103 32-33 1.0280706009076042 1103 34-35 0.8011378727786109 1103 36-37 0.8170700901112227 1103 38-39 0.8757284983009939 1103 40-41 0.8679235192736598 1103 42-43 1.030886912587846 1103 44-45 1.0013153930664842 1103 46-47 1.0215473864899565 1103 48-49 0.9738816710225464 1103 50-51 0.8722945040590133 1103 52-53 0.872151547169409 1103 54-55 0.7760145730771271 1103 56-57 0.8140719437351152 1103 58-59 0.8138391228543682 1103 60-61 0.7543294733987196 1103 62-63 0.8624332486581991 1103 64-65 0.90188386708747 1103 66-67 0.7686204510575934 1103 68-69 0.7861244754813939 1103 70-71 0.7949008911467104 1103 72-73 0.839994147078233 1103 74-75 0.9127647232501488 1103 76-77 0.8491853567854868 1103 78-79 0.7971486354747128 1103 80-81 0.7665260463559136 1103 82-83 0.7938185136789606 1103 84-85 0.856255870045846 1103 86-87 1.1537403961426342 1103 88-89 1.1301256594660813 1103 90-91 1.0868780130639486 1103 92-93 1.0056370909741723 1103 94-95 1.0107900306407132 1103 96-97 1.048329683318137 1103 98-99 1.2816830379783823 1103 100-101 1.7353397484619784 1104 1 0.4117016959827211 1104 2 0.3944854850825017 1104 3 0.41738104318536173 1104 4 0.42197461125220315 1104 5 0.3471068499862895 1104 6 0.2834394690280533 1104 7 0.36801128836629715 1104 8 0.3816798801165291 1104 9 0.5095983765659398 1104 10-11 0.49767421512728305 1104 12-13 0.46501327863043684 1104 14-15 0.7866887182430915 1104 16-17 0.7577353082684724 1104 18-19 0.7331347931306382 1104 20-21 0.6733508313895769 1104 22-23 0.7033181672255111 1104 24-25 0.4844350149804555 1104 26-27 0.6972534184300372 1104 28-29 0.6595432247332838 1104 30-31 0.7841813286683248 1104 32-33 0.5419990744296257 1104 34-35 0.5535029069041144 1104 36-37 0.5276963780888408 1104 38-39 0.8241060009951084 1104 40-41 0.7479147279402056 1104 42-43 0.8331758496872723 1104 44-45 0.7240853011828676 1104 46-47 0.8168286618950233 1104 48-49 0.7227366707389535 1104 50-51 0.9041542255722561 1104 52-53 1.1375653306568267 1104 54-55 1.0428161819856712 1104 56-57 1.14973840716128 1104 58-59 1.0900027929052243 1104 60-61 1.1059432266363913 1104 62-63 1.0478364447332922 1104 64-65 1.1433010914838846 1104 66-67 0.9934109479097302 1104 68-69 0.9966066044965771 1104 70-71 1.0682434175679703 1104 72-73 1.0326259698147062 1104 74-75 0.7271890090724753 1104 76-77 0.8273062801590925 1104 78-79 0.9118932614176565 1104 80-81 1.1291732775585288 1104 82-83 1.0771783155431045 1104 84-85 0.9699441180181658 1104 86-87 0.9459836986590346 1104 88-89 1.1740427420668027 1104 90-91 1.270591477038387 1104 92-93 0.8873085786946682 1104 94-95 0.7959990847688587 1104 96-97 0.9910879405544435 1104 98-99 1.1314321777511296 1104 100-101 1.2814960215037807 1105 1 0.34547454124102117 1105 2 0.34723992405439574 1105 3 0.33706743270330364 1105 4 0.3226072719154871 1105 5 0.3045961923148397 1105 6 0.3884321668232005 1105 7 0.3775372658861471 1105 8 0.47601135882779033 1105 9 0.4469856654080502 1105 10-11 0.5967796211239573 1105 12-13 0.37834320229725904 1105 14-15 0.4720647965747631 1105 16-17 0.5315656251309449 1105 18-19 0.6440949000325489 1105 20-21 0.6575316540849556 1105 22-23 0.7458397634224596 1105 24-25 0.7336001394135749 1105 26-27 0.7273958557700198 1105 28-29 0.5973240048570858 1105 30-31 0.5426586859205713 1105 32-33 0.484387930141601 1105 34-35 0.3362661558235658 1105 36-37 0.4598053849298509 1105 38-39 0.4813206976431914 1105 40-41 0.6173622437123782 1105 42-43 0.4617525997035372 1105 44-45 0.4508568988579853 1105 46-47 0.6152047343139699 1105 48-49 0.6529160625569332 1105 50-51 0.2673440178585338 1105 52-53 0.32187120730574037 1105 54-55 0.5116174378466525 1105 56-57 0.6588688239486586 1105 58-59 0.6692314199133307 1105 60-61 0.35539452654710857 1105 62-63 0.28535706533201477 1105 64-65 0.4134058056927401 1105 66-67 0.34537130905845004 1105 68-69 0.4192990190725965 1105 70-71 0.4675661158952664 1105 72-73 0.2247372234879812 1105 74-75 0.3844915095602701 1105 76-77 0.47575478335491894 1105 78-79 0.3831828840089635 1105 80-81 0.3128104259736233 1105 82-83 0.36180554833266143 1105 84-85 0.28965534094531264 1105 86-87 0.5145380986070016 1105 88-89 0.3500595814833396 1105 90-91 0.33120775947702796 1105 92-93 0.5480723834094654 1105 94-95 0.694999835683852 1105 96-97 0.6615542173760041 1105 98-99 0.39154617492485144 1105 100-101 0.4284069227791534 1106 1 0.5208813548157707 1106 2 0.4491357129730851 1106 3 0.5424656335213491 1106 4 0.5326655308287087 1106 5 0.5271118377312476 1106 6 0.6035585254610112 1106 7 0.5798001612788184 1106 8 0.4772328638660923 1106 9 0.5640667595883784 1106 10-11 0.6482541054228719 1106 12-13 0.6018245899007866 1106 14-15 0.8434684794288287 1106 16-17 0.715712258132541 1106 18-19 0.7366473215926064 1106 20-21 0.7200235898593306 1106 22-23 0.7739723671275769 1106 24-25 0.6842146579670256 1106 26-27 0.8703489506315165 1106 28-29 0.5748116342531233 1106 30-31 0.7645174039624933 1106 32-33 0.8240164354189616 1106 34-35 0.8464829156586404 1106 36-37 0.8650244535076936 1106 38-39 1.023143327252079 1106 40-41 0.9821799512781837 1106 42-43 0.9169043708858382 1106 44-45 0.8828092437874275 1106 46-47 0.5720079934073041 1106 48-49 0.6120460758854236 1106 50-51 0.6054811735529384 1106 52-53 0.7477022728314537 1106 54-55 0.532900116381235 1106 56-57 0.69227322071184 1106 58-59 0.7907380956184795 1106 60-61 0.9471806419973419 1106 62-63 0.9933086374057254 1106 64-65 0.7956468253383377 1106 66-67 0.7091685850694702 1106 68-69 0.7602050286809003 1106 70-71 0.7048852234433767 1106 72-73 0.7402038351378017 1106 74-75 0.6843372658945768 1106 76-77 0.8344836321601008 1106 78-79 0.990515189051191 1106 80-81 0.9974656373463944 1106 82-83 1.0843660586794321 1106 84-85 1.2896916913633447 1106 86-87 1.2442950092799414 1106 88-89 1.3165448235411006 1106 90-91 1.1120022944700345 1106 92-93 1.1825525560575763 1106 94-95 1.2470483946026434 1106 96-97 1.0094961615011542 1106 98-99 0.9257994030712098 1106 100-101 1.1522803054311623 1107 1 0.3003150960815759 1107 2 0.3988221184937366 1107 3 0.4970968498755717 1107 4 0.5131978625060825 1107 5 0.25055439541589664 1107 6 0.36071465339139763 1107 7 0.41764267742013317 1107 8 0.48745137026780583 1107 9 0.4483339524706196 1107 10-11 0.6306154978169758 1107 12-13 0.5608726705410163 1107 14-15 0.36354198805195637 1107 16-17 0.4672196464992524 1107 18-19 0.5239829513491756 1107 20-21 0.5143292680254277 1107 22-23 0.5883169344710595 1107 24-25 0.5601327945176635 1107 26-27 0.5009306150190653 1107 28-29 0.49072965540560176 1107 30-31 0.5660902807807346 1107 32-33 0.5166303766697666 1107 34-35 0.6240702864848373 1107 36-37 0.6740318562991803 1107 38-39 0.6583957318610771 1107 40-41 0.75104899168484 1107 42-43 0.7627699435780144 1107 44-45 0.8390185298767605 1107 46-47 0.7949947283896819 1107 48-49 0.5521602046582217 1107 50-51 0.44039257662137743 1107 52-53 0.4492884204372345 1107 54-55 0.4802064350070836 1107 56-57 0.5745988396786785 1107 58-59 0.5724999946104745 1107 60-61 0.46200930459045964 1107 62-63 0.46407251547603323 1107 64-65 0.5478667972965852 1107 66-67 0.6275820912692254 1107 68-69 0.8054382194975176 1107 70-71 0.8279775477352658 1107 72-73 0.6797207784715376 1107 74-75 0.5874230124917688 1107 76-77 0.5903040264755894 1107 78-79 0.6533939113628442 1107 80-81 0.7798795358998802 1107 82-83 0.8628535350949349 1107 84-85 0.7815676614290616 1107 86-87 0.6236635505895975 1107 88-89 0.6002771459866167 1107 90-91 0.6076719787983897 1107 92-93 0.6770278695078069 1107 94-95 0.7058801037069777 1107 96-97 0.9391869521515943 1107 98-99 0.9117929523144852 1107 100-101 0.9813006328157208 1108 1 0.06898866938611903 1108 2 0.24134169586962884 1108 3 0.19244126839551612 1108 4 0.017076146413309345 1108 5 -0.011799943328767881 1108 6 0.08363804046611989 1108 7 -0.05506111707402539 1108 8 -0.2568613733213496 1108 9 -0.1026011345405422 1108 10-11 0.027574619140374068 1108 12-13 -0.21137345254240358 1108 14-15 0.06890726649972834 1108 16-17 0.11634734710514039 1108 18-19 0.239272556498193 1108 20-21 0.31454432108314734 1108 22-23 0.2587730088448481 1108 24-25 0.34189906269079273 1108 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2316 100-101 -3.030067602677139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3529.0 3 1158.0 4 1163.0 5 1273.0 6 1490.0 7 1633.0 8 2098.0 9 2519.0 10 3171.0 11 4113.0 12 5168.0 13 6499.0 14 7223.0 15 8958.0 16 11323.0 17 14531.0 18 12489.0 19 8687.0 20 8102.0 21 8750.0 22 9351.0 23 10357.0 24 11906.0 25 13412.0 26 16048.0 27 18487.0 28 21850.0 29 26576.0 30 32372.0 31 39769.0 32 49953.0 33 64268.0 34 86621.0 35 120758.0 36 175064.0 37 250637.0 38 222078.0 39 39321.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21970215006263 19.42582798288664 14.903162461951819 21.45130740509891 2 14.960869453352846 21.182860956780083 40.454749771845684 23.40151981802139 3 19.670301904263336 26.074533466073973 29.983372116597927 24.271792513064767 4 12.245373752084433 17.202973575868043 38.826344350137724 31.725308321909797 5 13.102725446200534 38.48399656434104 33.86220283252676 14.55107515693167 6 29.74146966432602 37.265207188267865 18.07222624563825 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27.376153021553833 30.193448136820056 21.23783809546145 30-31 21.628224658454343 26.656180648948098 30.048491843900283 21.66710284869728 32-33 21.4938136579064 26.842250167535735 30.0664243260374 21.59751184852047 34-35 22.140881183382273 26.76814337335045 29.80771739808682 21.283258045180457 36-37 21.939888478423327 26.45627029005897 30.21411023269464 21.389730998823065 38-39 21.592998202427196 27.10870644724655 30.020751697823204 21.277543652503045 40-41 21.79384905302106 27.020379971729263 29.864096957207696 21.321674018041982 42-43 21.422389892193788 27.155424465678045 29.262339540770206 22.15984610135796 44-45 21.801728617491758 26.93092866862265 29.193886681536984 22.07345603234861 46-47 22.284969230268914 27.348153627061933 28.837405586424865 21.529471556244285 48-49 22.162794426636694 27.33215545030579 28.627965444058372 21.877084678999143 50-51 21.586110960917416 27.628951580367474 28.99351276669516 21.79142469201995 52-53 22.052332555335887 28.091254108688712 27.957915531561838 21.898497804413562 54-55 21.731295485865818 27.52614373647053 28.384050089477913 22.35851068818574 56-57 22.178140983651197 27.704201189966522 27.93187721530285 22.185780611079426 58-59 22.66100942952704 27.351528728645892 28.08759236726856 21.899869474558507 60-61 22.213531600537674 27.475761864526653 28.832923470503115 21.477783064432554 62-63 21.890860208139642 27.60044057762268 28.774658616643755 21.73404059759392 64-65 21.661359057934433 28.03351489250343 28.417356920520497 21.88776912904164 66-67 21.95989418285608 27.756310410067908 27.84428963395953 22.439505773116483 68-69 21.713326795380873 27.67618905701067 28.341721352365255 22.268762795243205 70-71 22.261376680847974 27.557891043392384 28.051010361479676 22.12972191427997 72-73 21.992327980714848 27.26005468563322 28.61498350054595 22.13263383310598 74-75 21.621573590433314 28.385194719357138 27.88649506669621 22.106736623513335 76-77 21.904532483415483 27.63981403256926 27.922947706303365 22.532705777711893 78-79 21.851287020709886 27.78439689272268 28.047119020764356 22.31719706580308 80-81 22.170024477855538 27.99252466787751 27.671926399289674 22.16552445497727 82-83 22.0273569509084 27.770387698638583 28.0863939544656 22.11586139598742 84-85 21.930470561135273 27.96870868874019 27.778414487323367 22.322406262801174 86-87 21.833100647146246 28.107911418619157 27.851269460439127 22.207718473795477 88-89 21.948892220858916 27.861537349782317 27.435141034270234 22.754429395088536 90-91 21.9490432368438 27.566239631440986 27.821666458633565 22.66305067308165 92-93 22.246028710776507 27.847047346036767 27.84054091166494 22.066383031521784 94-95 21.932477099483556 27.72671539650911 27.82337556998291 22.51743193402442 96-97 21.467627358219303 27.834441125104803 28.1567147012443 22.5412168154316 98-99 21.680080083110827 28.029694333060206 27.43940308562194 22.850822498207023 100-101 21.693863958038463 27.69026119996064 27.74127504333149 22.8745997986694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 555.0 1 398.0 2 267.0 3 327.0 4 320.5 5 341.5 6 531.0 7 1054.5 8 1733.0 9 2085.0 10 1948.0 11 1699.5 12 1717.0 13 1853.0 14 2033.0 15 2147.0 16 2174.0 17 2270.0 18 2425.0 19 2580.5 20 2865.0 21 3298.5 22 3957.5 23 4724.5 24 5477.5 25 6668.5 26 8357.0 27 10282.5 28 11964.0 29 13843.5 30 16336.0 31 18960.0 32 21707.5 33 24318.5 34 27435.5 35 30484.5 36 33936.5 37 37498.5 38 39247.5 39 40098.5 40 40624.5 41 41519.0 42 42010.5 43 42915.5 44 44954.5 45 46412.0 46 47460.5 47 52490.5 48 64392.5 49 63707.0 50 51167.0 51 45020.0 52 42643.0 53 41069.0 54 39071.5 55 36181.0 56 32547.5 57 28841.5 58 26157.5 59 23405.5 60 19814.5 61 16147.5 62 12484.5 63 9228.5 64 6577.0 65 4725.5 66 3404.5 67 2505.0 68 1769.0 69 1176.0 70 829.0 71 554.5 72 368.5 73 280.5 74 197.5 75 139.0 76 103.5 77 47.5 78 20.5 79 17.0 80 17.0 81 15.0 82 10.0 83 9.0 84 6.5 85 4.0 86 4.5 87 4.0 88 4.0 89 4.0 90 3.0 91 1.5 92 2.5 93 2.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05322398224254411 2 0.09359558240947387 3 0.06320347891302112 4 0.12164401623331458 5 0.09654407006211481 6 0.09684647905212926 7 0.10516272627752678 8 0.09185673071689075 9 0.11143771282032672 10-11 0.1041798970599798 12-13 0.08735839699042573 14-15 0.08966426553928596 16-17 0.10637236223758459 18-19 0.08452331270904022 20-21 0.06898705084704758 22-23 0.072426953108462 24-25 0.05855394069154888 26-27 0.05178753953997545 28-29 0.06494233060560425 30-31 0.047931824917291144 32-33 0.045814961987189956 34-35 0.04305547995330805 36-37 0.04808302941229837 38-39 0.04925486424860438 40-41 0.06429971150182352 42-43 0.06070860474540188 44-45 0.04649538221472248 46-47 0.0356086585742021 48-49 0.042790872087045406 50-51 0.026120576512498564 52-53 0.03885955521685749 54-55 0.04521014400716104 56-57 0.04974627885737787 58-59 0.05651268000895131 60-61 0.05481162944012 62-63 0.06131342272543078 64-65 0.041845843993250226 66-67 0.06116221823042355 68-69 0.05197654515873448 70-71 0.023739105716134728 72-73 0.018446948390881766 74-75 0.028199638318847944 76-77 0.041203224889469516 78-79 0.0591965597953296 80-81 0.03753651588554425 82-83 0.05628587326644047 84-85 0.06135122384918259 86-87 0.06286326879925486 88-89 0.08856803295048356 90-91 0.06101101373541633 92-93 0.07133072051965961 94-95 0.08100780820012218 96-97 0.08800101609420645 98-99 0.11979176116947605 100-101 0.1135545757504279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1322712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.72612789163829 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13194558884238 50.890927951031664 2 11.492396233167765 14.417470318061268 3 3.330695331372888 6.267648639713351 4 1.4059825809596072 3.5276737274675196 5 0.711528960003631 2.2315728271896074 6 0.3993725944502722 1.50306578615419 7 0.2887759899200313 1.2679659773030831 8 0.19330566072078476 0.9700252477239667 9 0.15548670590716504 0.8777771100164147 >10 0.8115007681083832 9.241812390600865 >50 0.05336980927231277 2.2718906078558136 >100 0.021890598107532667 2.35544785832114 >500 0.001693177688455334 0.7094592879318427 >1k 0.001693177688455334 2.05866089570214 >5k 3.628237903832858E-4 1.408601374927123 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7022 0.530878981970376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6321 0.47788180647034273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.395021743206382 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3954 0.2989312866292889 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.25803047072983387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2995 0.22642873127332327 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2318 0.17524600971337675 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2111 0.15959634448012872 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1784 0.134874409546447 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1760 0.13305995560636028 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1626 0.12292925444087602 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1345 0.10168502289236055 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1328 0.1003997846847991 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.56022475036138E-5 0.0 0.0 0.0 0.0 6 7.56022475036138E-5 0.0 0.0 0.0 0.0 7 2.2680674251084136E-4 7.56022475036138E-5 0.0 0.0 0.0 8 2.2680674251084136E-4 7.56022475036138E-5 0.0 0.0 0.0 9 2.2680674251084136E-4 7.56022475036138E-5 0.0 0.0 0.0 10-11 2.2680674251084136E-4 7.56022475036138E-5 0.0 0.0 0.0 12-13 2.2680674251084136E-4 7.56022475036138E-5 0.0 3.78011237518069E-5 0.0 14-15 2.2680674251084136E-4 7.56022475036138E-5 0.0 7.56022475036138E-5 0.0 16-17 3.024089900144552E-4 7.56022475036138E-5 0.0 7.56022475036138E-5 0.0 18-19 3.7801123751806894E-4 7.56022475036138E-5 0.0 7.56022475036138E-5 0.0 20-21 3.7801123751806894E-4 1.512044950072276E-4 0.0 1.8900561875903447E-4 0.0 22-23 3.7801123751806894E-4 1.512044950072276E-4 0.0 3.024089900144552E-4 0.0 24-25 6.048179800289104E-4 1.512044950072276E-4 0.0 6.426191037807172E-4 0.0 26-27 6.048179800289104E-4 1.512044950072276E-4 0.0 8.694258462915586E-4 0.0 28-29 6.426191037807172E-4 1.512044950072276E-4 0.0 0.002154664053852993 0.0 30-31 7.560224750361379E-4 2.2680674251084138E-4 0.0 0.0040447202414433375 0.0 32-33 7.560224750361379E-4 3.4021011376626206E-4 0.0 0.007749230369120413 0.0 34-35 7.938235987879447E-4 3.7801123751806894E-4 0.0 0.011264734878038455 0.0 36-37 9.072269700433654E-4 3.7801123751806894E-4 0.0 0.015649665233248053 0.0 38-39 0.001058431465050593 3.7801123751806894E-4 0.0 0.01886276075215164 0.0 40-41 0.001058431465050593 3.7801123751806894E-4 0.0 0.02158444166228174 0.0 42-43 0.0010962325888024 3.7801123751806894E-4 0.0 0.02457073043867448 0.0 44-45 0.0011340337125542068 3.7801123751806894E-4 0.0 0.02702780348254193 0.0 46-47 0.0011718348363060137 3.7801123751806894E-4 0.0 0.032546767550305736 0.0 48-49 0.0012096359600578207 3.7801123751806894E-4 0.0 0.03776332262805508 0.0 50-51 0.0012096359600578207 3.7801123751806894E-4 0.0 0.04373590018084057 0.0 52-53 0.0012096359600578207 3.7801123751806894E-4 0.0 0.053110578871288686 0.0 54-55 0.0012096359600578207 3.7801123751806894E-4 0.0 0.07212454411844756 0.0 56-57 0.0012096359600578207 4.1581236126987583E-4 0.0 0.10943425326148096 0.0 58-59 0.0012096359600578207 4.536134850216827E-4 0.0 0.14742438263204688 0.0 60-61 0.0012096359600578207 4.536134850216827E-4 0.0 0.18609493223014534 0.0 62-63 0.0012096359600578207 4.536134850216827E-4 0.0 0.23553880209750877 0.0 64-65 0.0012096359600578207 4.536134850216827E-4 0.0 0.29590719672914434 0.0 66-67 0.0012852382075614344 4.536134850216827E-4 0.0 0.39063681285117247 0.0 68-69 0.0012852382075614344 4.536134850216827E-4 0.0 0.5072154785017449 0.0 70-71 0.0012852382075614344 4.536134850216827E-4 0.0 0.6047045766576549 0.0 72-73 0.0012852382075614344 4.536134850216827E-4 0.0 0.7012486467197697 0.0 74-75 0.0012852382075614344 4.536134850216827E-4 0.0 0.8066381797398072 0.0 76-77 0.0012852382075614344 4.536134850216827E-4 0.0 0.9304368600269749 0.0 78-79 0.0012852382075614344 4.536134850216827E-4 0.0 1.029248997514198 0.0 80-81 0.0013608404550650482 4.536134850216827E-4 0.0 1.135961569865549 0.0 82-83 0.001436442702568662 4.536134850216827E-4 0.0 1.2544302917037118 0.0 84-85 0.0015120449500722758 4.536134850216827E-4 0.0 1.4413946497801486 0.0 86-87 0.0015876471975758894 4.536134850216827E-4 0.0 1.6803733541390717 0.0 88-89 0.0015876471975758894 4.536134850216827E-4 0.0 1.9577958013535826 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7755 0.0 24.207268 1 TACATGG 7835 0.0 23.414171 2 ACATGGG 7790 0.0 22.817322 3 GAGTACT 5290 0.0 22.094625 12-13 CATGGGA 5295 0.0 20.464417 4 GTACTTT 5780 0.0 20.262268 14-15 AGTACTT 5795 0.0 19.80026 12-13 TTTGCGT 200 1.2789418E-5 19.010366 3 GTATCAA 16155 0.0 18.298433 1 AGAGTAC 10920 0.0 18.1255 10-11 CATGGGG 2675 0.0 17.411364 4 ACTGCGT 165 0.0014582606 17.282806 8 GACCGAC 165 0.0014586304 17.282152 7 ACTTTTT 6960 0.0 17.27202 16-17 ATGGGAG 2535 0.0 17.060585 5 TACTTTT 6835 0.0 16.717663 14-15 ATGGGAT 2135 0.0 16.472683 5 CAACGCA 18040 0.0 16.281052 5 TCAACGC 18040 0.0 16.281052 4 TATCAAC 18080 0.0 16.245031 2 >>END_MODULE