FastQCFastQC Report
Thu 26 May 2016
SRR936887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322712
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80660.6098077283641489No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71470.5403292629083277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.48400558851813547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.32319960807794895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33400.25251150666207006No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27650.2090402143474921No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22180.1676857849630154No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21470.1623180253902588No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21030.15899152650009982No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18340.1386545219216277No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16330.12345847017340131No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.12119040274829292No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13960.10554073751504484No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13910.10516272627752678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13760.10402869256497257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71800.022.763061
GAGTACT70650.022.25261512-13
TACATGG74200.021.7017082
ACATGGG72750.021.349933
GTACTTT75050.021.04332714-15
AGTACTT74000.020.21827512-13
CATGGGA49300.019.3648624
ATGGGAT20150.019.3287645
ACCGTGC1300.007566562418.2680388
AGAGTAC124400.018.11678310-11
ACTTTTT89000.018.03883216-17
TACTTTT85400.017.46406214-15
CCTATAC3551.5843398E-917.393893
GTAGCAC3301.21090125E-817.2722533
CATGGGG25000.017.0988834
GTATCAA177200.016.9989511
GCGGTAT1750.002166534816.2895771
GGTATCA146200.016.1187341
TCCGTGC1800.0026200315.8322998
AGTACAT57700.015.641412
Produced by FastQC (version 0.11.5)