##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1322712 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04772769884903 31.0 31.0 34.0 30.0 34.0 2 32.0209387984686 33.0 31.0 34.0 30.0 34.0 3 32.20251800845536 33.0 31.0 34.0 30.0 34.0 4 35.95504161147703 37.0 35.0 37.0 35.0 37.0 5 35.87762188594343 37.0 35.0 37.0 35.0 37.0 6 35.93218175989936 37.0 35.0 37.0 35.0 37.0 7 35.8666262950665 37.0 35.0 37.0 35.0 37.0 8 35.85150055340845 37.0 35.0 37.0 35.0 37.0 9 37.509749665838065 39.0 37.0 39.0 35.0 39.0 10-11 37.43859094043148 39.0 37.0 39.0 34.0 39.0 12-13 37.34322097327309 39.0 37.0 39.0 34.0 39.0 14-15 38.47918670126225 40.0 38.0 41.0 34.0 41.0 16-17 38.41238304332312 40.0 38.0 41.0 34.0 41.0 18-19 38.51140724511458 40.0 38.0 41.0 34.0 41.0 20-21 38.61012487979242 40.0 38.0 41.0 34.0 41.0 22-23 38.53759057149251 40.0 38.0 41.0 34.0 41.0 24-25 38.432616094811266 40.0 38.0 41.0 34.0 41.0 26-27 38.2645761133187 40.0 38.0 41.0 34.0 41.0 28-29 38.09428016076062 40.0 37.5 41.0 33.0 41.0 30-31 37.945215965380214 40.0 37.0 41.0 33.0 41.0 32-33 37.89023763298435 40.0 37.0 41.0 33.0 41.0 34-35 37.786966852950606 40.0 37.0 41.0 33.0 41.0 36-37 37.60883434942754 40.0 37.0 41.0 33.0 41.0 38-39 37.44131715747646 40.0 36.5 41.0 33.0 41.0 40-41 37.26672586322646 40.0 36.0 41.0 32.0 41.0 42-43 37.01671830300171 39.5 36.0 41.0 31.0 41.0 44-45 36.66193472199542 39.0 36.0 41.0 30.5 41.0 46-47 36.33093901015489 39.0 35.5 41.0 30.0 41.0 48-49 35.959074613370106 39.0 35.0 41.0 29.0 41.0 50-51 35.69080495224962 39.0 35.0 40.0 28.0 41.0 52-53 35.43738206049389 39.0 35.0 40.0 27.5 41.0 54-55 35.21068872135431 38.5 35.0 40.0 27.0 41.0 56-57 34.93545760528369 38.0 34.0 40.0 26.0 41.0 58-59 34.61491466018302 38.0 34.0 40.0 26.0 41.0 60-61 34.263962223068965 38.0 34.0 40.0 25.5 41.0 62-63 33.91460348133229 37.0 33.0 40.0 24.5 41.0 64-65 33.51551849533383 36.5 33.0 39.0 23.0 41.0 66-67 33.12143611005268 36.0 32.5 39.0 22.5 40.0 68-69 32.66878768772038 35.5 32.0 39.0 22.0 40.0 70-71 32.18422226455948 35.0 31.0 38.0 20.5 40.0 72-73 31.835334146813516 35.0 31.0 37.0 19.5 39.5 74-75 31.399389285044663 35.0 31.0 37.0 18.0 39.0 76-77 29.99706512075191 33.5 29.5 35.5 17.5 37.5 78-79 30.716326002939418 34.0 30.5 36.0 18.0 38.0 80-81 30.537189123558264 34.0 31.0 35.5 14.5 37.0 82-83 30.199043707171327 34.0 30.0 35.0 10.0 37.0 84-85 29.808358508881753 34.0 30.0 35.0 7.0 36.5 86-87 29.46361339429899 34.0 29.5 35.0 4.5 36.0 88-89 29.095249381573616 34.0 29.0 35.0 2.0 36.0 90-91 28.662597753706024 34.0 29.0 35.0 2.0 35.0 92-93 28.203709121864776 33.5 29.0 35.0 2.0 35.0 94-95 27.71905789015296 33.0 27.0 35.0 2.0 35.0 96-97 27.076660678968665 33.0 26.5 35.0 2.0 35.0 98-99 26.34100431537629 33.0 25.0 34.5 2.0 35.0 100-101 23.284178264051434 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39529261102281765 1101 2 0.3362216010695249 1101 3 0.32495441580433493 1101 4 0.2698162910653039 1101 5 0.3716553742788591 1101 6 0.3527200310464451 1101 7 0.35602789163863946 1101 8 0.36773166001785995 1101 9 0.4318512796732108 1101 10-11 0.4648237299981872 1101 12-13 0.47360611551653875 1101 14-15 0.5917670395352985 1101 16-17 0.5928978405145884 1101 18-19 0.5143892257580873 1101 20-21 0.5773692305241624 1101 22-23 0.5611234331797732 1101 24-25 0.5379223395831474 1101 26-27 0.6596358597864551 1101 28-29 0.6085850795759811 1101 30-31 0.7501249923721716 1101 32-33 0.7427668775860212 1101 34-35 0.6114426815631973 1101 36-37 0.6604807694308761 1101 38-39 0.6745033954771102 1101 40-41 0.6163721548794712 1101 42-43 0.8464607334072483 1101 44-45 0.9766101303484831 1101 46-47 0.8643430890481838 1101 48-49 0.5992693998521119 1101 50-51 0.7623452205124437 1101 52-53 0.5848350779334197 1101 54-55 0.5624172296433869 1101 56-57 0.4859031873036841 1101 58-59 0.48014623021365566 1101 60-61 0.5552681755982078 1101 62-63 0.7233696233622524 1101 64-65 0.6177111061694376 1101 66-67 0.643578710926505 1101 68-69 0.6027237311795659 1101 70-71 0.5424310224433526 1101 72-73 0.6699885311141465 1101 74-75 0.5690404225482304 1101 76-77 0.3134583021337569 1101 78-79 0.28940935740569174 1101 80-81 0.571985093668939 1101 82-83 0.6574138713887017 1101 84-85 0.6169337200844183 1101 86-87 0.5795370071107939 1101 88-89 0.677765248591907 1101 90-91 0.8538780016132144 1101 92-93 0.884335816911193 1101 94-95 1.1054582926505674 1101 96-97 0.718506189602369 1101 98-99 0.8620445337565066 1101 100-101 1.22763200761651 1102 1 0.35056949443763585 1102 2 0.26829260497065377 1102 3 0.25994132687342386 1102 4 0.24781141323812506 1102 5 0.2618453483281371 1102 6 0.2744377189904341 1102 7 0.3702695570276475 1102 8 0.3357674118222036 1102 9 0.42331508210700974 1102 10-11 0.35988052914414936 1102 12-13 0.3912086691357288 1102 14-15 0.5473118769315874 1102 16-17 0.45789446181007776 1102 18-19 0.3863320660277054 1102 20-21 0.46784064498047684 1102 22-23 0.4764819165673373 1102 24-25 0.4909313740686514 1102 26-27 0.4213033924235958 1102 28-29 0.45553650856923866 1102 30-31 0.4977141163507497 1102 32-33 0.5867118983976951 1102 34-35 0.5434455435246619 1102 36-37 0.586827495494596 1102 38-39 0.7084693131502888 1102 40-41 0.7726656308566007 1102 42-43 0.7534079461179886 1102 44-45 0.8025433200158929 1102 46-47 0.566927656034558 1102 48-49 0.7008030193815955 1102 50-51 0.8025851653174669 1102 52-53 0.6973764599655254 1102 54-55 0.6223104455454376 1102 56-57 0.7227958012852795 1102 58-59 0.650277133718987 1102 60-61 0.6157029779581862 1102 62-63 0.7630580443431612 1102 64-65 0.7599762152249312 1102 66-67 0.5424784315445521 1102 68-69 0.6071987401232022 1102 70-71 0.3979885654721542 1102 72-73 0.5424037511842208 1102 74-75 0.42580145160508565 1102 76-77 0.5491044864690586 1102 78-79 0.7376296461003733 1102 80-81 0.6316603973264598 1102 82-83 0.661250759459886 1102 84-85 0.665216543204977 1102 86-87 0.4918692075843829 1102 88-89 0.6091495095073931 1102 90-91 0.7304221973395784 1102 92-93 0.6172559687541508 1102 94-95 0.7225367921650552 1102 96-97 0.894833040933694 1102 98-99 0.9472773236578718 1102 100-101 1.0101978213732288 1103 1 0.09898089603530735 1103 2 -0.16448698834815545 1103 3 0.03976158831132892 1103 4 0.10111569791495612 1103 5 0.23542922632377383 1103 6 0.20354084172100784 1103 7 0.3082688308112296 1103 8 0.3052209339718033 1103 9 0.27680092088696995 1103 10-11 0.24994951970333545 1103 12-13 0.37648009251989834 1103 14-15 0.3280852974108939 1103 16-17 0.4517216223039142 1103 18-19 0.34816031584615104 1103 20-21 0.36446827751498034 1103 22-23 0.4692424467053229 1103 24-25 0.4047839521369099 1103 26-27 0.35369718327327604 1103 28-29 0.2861011418299526 1103 30-31 0.3333168759731251 1103 32-33 0.4755100102350269 1103 34-35 0.33710930532568284 1103 36-37 0.23184383536388253 1103 38-39 0.19555627756567873 1103 40-41 0.15111515881593363 1103 42-43 0.10580446027920942 1103 44-45 0.27295000120688684 1103 46-47 0.12295979111080868 1103 48-49 0.44544717333947403 1103 50-51 0.22453959523758726 1103 52-53 0.3564859370897153 1103 54-55 0.3968429437298937 1103 56-57 0.3982224534276142 1103 58-59 0.43779075027672576 1103 60-61 0.36066485159653183 1103 62-63 0.4431597146409061 1103 64-65 0.3514567889358986 1103 66-67 0.5099348585598023 1103 68-69 0.46095691005784545 1103 70-71 0.39864669909597694 1103 72-73 0.524702226129758 1103 74-75 0.452807624444592 1103 76-77 0.4085135278633949 1103 78-79 0.6634647949747396 1103 80-81 0.6593473980526774 1103 82-83 0.7305363440640988 1103 84-85 0.7300767465455706 1103 86-87 0.6088127442583122 1103 88-89 1.0479884710179235 1103 90-91 0.9382108683635444 1103 92-93 1.072434799545725 1103 94-95 0.9912141705238042 1103 96-97 1.2277124890092175 1103 98-99 1.4386988922853234 1103 100-101 1.4616320987878986 1104 1 0.33354525237736254 1104 2 0.17417195517933948 1104 3 0.15188299379031633 1104 4 0.21089156617729543 1104 5 0.2191227207370261 1104 6 0.16870400130734708 1104 7 0.26697555948988594 1104 8 0.22580234361012685 1104 9 0.267640918618234 1104 10-11 0.29773585248838685 1104 12-13 0.30460798751545326 1104 14-15 0.29798561503326937 1104 16-17 0.33087387307433147 1104 18-19 0.3754571698721989 1104 20-21 0.2779947602253543 1104 22-23 0.39165597496047866 1104 24-25 0.37642717524771996 1104 26-27 0.2619252332987969 1104 28-29 0.45607637484755514 1104 30-31 0.2954018872222477 1104 32-33 0.40360777364417544 1104 34-35 0.40183667945836277 1104 36-37 0.4415596767795691 1104 38-39 0.3712299253073539 1104 40-41 0.43782842900291996 1104 42-43 0.3308271475913287 1104 44-45 0.2873576260343711 1104 46-47 0.4928002000496079 1104 48-49 0.5221066556906351 1104 50-51 0.42355399695426144 1104 52-53 0.35089741403373154 1104 54-55 0.28404387296438927 1104 56-57 0.21012927474612297 1104 58-59 0.22313155624348724 1104 60-61 0.18183565869765772 1104 62-63 0.25624934842987557 1104 64-65 0.009166103022828054 1104 66-67 0.20704506217843033 1104 68-69 0.18014824917644034 1104 70-71 0.22210100779466302 1104 72-73 0.3241570216603513 1104 74-75 0.32344268012303345 1104 76-77 0.26054439678744856 1104 78-79 0.12704338760514133 1104 80-81 0.32915011296769237 1104 82-83 0.28683691786403287 1104 84-85 0.3717294699683329 1104 86-87 0.34861822418949373 1104 88-89 0.5173541006853632 1104 90-91 0.6388269706833221 1104 92-93 0.7330332010355249 1104 94-95 0.7292141185320489 1104 96-97 0.915748949410407 1104 98-99 1.0710049157666894 1104 100-101 1.1769689581076541 1105 1 0.14621324743432496 1105 2 0.046996518968683176 1105 3 0.049226410276155264 1105 4 0.14672440185699287 1105 5 0.11661040750495744 1105 6 0.08164871149554642 1105 7 0.0968347777105123 1105 8 0.144736044320247 1105 9 0.19170153578758686 1105 10-11 0.22733106791821456 1105 12-13 0.2762766152330869 1105 14-15 0.2939779758035712 1105 16-17 0.3508588547744793 1105 18-19 0.36776265628181903 1105 20-21 0.2907180391814066 1105 22-23 0.398611398990937 1105 24-25 0.3166398556594885 1105 26-27 0.2186666169926994 1105 28-29 0.272125261187405 1105 30-31 0.4223529358425111 1105 32-33 0.3480122744039562 1105 34-35 0.39050436705407776 1105 36-37 0.16880222232226316 1105 38-39 0.4300404641332065 1105 40-41 0.16073311010055136 1105 42-43 0.1955048749796262 1105 44-45 0.3401070391972638 1105 46-47 0.399792874193885 1105 48-49 0.39288985411548794 1105 50-51 0.3523069459216046 1105 52-53 0.4999838993376784 1105 54-55 0.43246576893605493 1105 56-57 0.6270757114475387 1105 58-59 0.41700657324254564 1105 60-61 0.41638306981474926 1105 62-63 0.41953847435299707 1105 64-65 0.4826561430519192 1105 66-67 0.4907513893763351 1105 68-69 0.45375506535599186 1105 70-71 0.25162497499091785 1105 72-73 0.3362012251287574 1105 74-75 0.3643096026132362 1105 76-77 0.2358685614684255 1105 78-79 0.29389462957124124 1105 80-81 0.5564313868033324 1105 82-83 0.516982015926434 1105 84-85 0.6810807267162176 1105 86-87 0.5745126703749044 1105 88-89 0.5569852654313827 1105 90-91 0.6676313602840374 1105 92-93 0.7907945283221203 1105 94-95 0.6299919363849042 1105 96-97 0.8190580011880648 1105 98-99 0.7524215435079711 1105 100-101 1.00277264409511 1106 1 0.3987346892687498 1106 2 0.23069843244159216 1106 3 0.2391360582010691 1106 4 0.2363173754194321 1106 5 0.18043912171535226 1106 6 0.1954949459524684 1106 7 0.2646466462857404 1106 8 0.22984514469651884 1106 9 0.34055551517591454 1106 10-11 0.39326188423071073 1106 12-13 0.3855302527309945 1106 14-15 0.39274107914683043 1106 16-17 0.4036111708855685 1106 18-19 0.4376841590277536 1106 20-21 0.3578801849695026 1106 22-23 0.5225391435293716 1106 24-25 0.5119486868461749 1106 26-27 0.4489845030357813 1106 28-29 0.47434918631209655 1106 30-31 0.5324675215296182 1106 32-33 0.40950539238562556 1106 34-35 0.4704025858835905 1106 36-37 0.38769625266667873 1106 38-39 0.5940902281829707 1106 40-41 0.348540000960881 1106 42-43 0.37633739779688113 1106 44-45 0.33724059587280664 1106 46-47 0.07958793643170026 1106 48-49 0.008447833032242613 1106 50-51 -0.08490049052247173 1106 52-53 -0.08278993229112075 1106 54-55 -0.008252593919706896 1106 56-57 0.10662057801530977 1106 58-59 0.03396158714412678 1106 60-61 -0.08959313539809699 1106 62-63 0.0029797127560655667 1106 64-65 0.03480720359993228 1106 66-67 0.05439343927793061 1106 68-69 -0.008243683665654089 1106 70-71 -0.04791524134319047 1106 72-73 0.018146529211460205 1106 74-75 0.15740662548125073 1106 76-77 0.049902770159125254 1106 78-79 -0.007802050751394063 1106 80-81 0.014462373078593771 1106 82-83 0.009006210240706025 1106 84-85 0.271101390782686 1106 86-87 -0.0366288964918553 1106 88-89 0.06550272509388222 1106 90-91 0.39041259298893394 1106 92-93 0.517111903494456 1106 94-95 0.4746011038948339 1106 96-97 0.8580901631209876 1106 98-99 1.0440318015304442 1106 100-101 0.9505292338364306 1107 1 0.21804426212248273 1107 2 0.1593823742116811 1107 3 0.1751073932999958 1107 4 0.1973301096055593 1107 5 0.18969982345151948 1107 6 0.2390346688815015 1107 7 0.20833811778528144 1107 8 0.2813011808853787 1107 9 0.3187510342656523 1107 10-11 0.24076899564185794 1107 12-13 0.24655914265846945 1107 14-15 0.35043034654165695 1107 16-17 0.4204794958236917 1107 18-19 0.4786245385722765 1107 20-21 0.34835118252883035 1107 22-23 0.4039473471840296 1107 24-25 0.4523715056768509 1107 26-27 0.3403515672490798 1107 28-29 0.36483633961277206 1107 30-31 0.4511204788957741 1107 32-33 0.48492673988985047 1107 34-35 0.572968463803889 1107 36-37 0.45198744836463334 1107 38-39 0.3896306665805511 1107 40-41 0.4396324175712891 1107 42-43 0.3953925177244102 1107 44-45 0.4905268467599271 1107 46-47 0.5235880979891121 1107 48-49 0.28079612773604623 1107 50-51 0.15433141937464967 1107 52-53 0.10561197639432152 1107 54-55 -2.1820694385610295E-5 1107 56-57 0.12849141286324084 1107 58-59 0.3540558674346954 1107 60-61 0.20746190375071905 1107 62-63 0.030398313784267827 1107 64-65 0.12856531753252654 1107 66-67 0.07059245493168476 1107 68-69 0.14751208768444712 1107 70-71 0.224130133210366 1107 72-73 0.34400494721819186 1107 74-75 0.25237930496865246 1107 76-77 0.2513187054900037 1107 78-79 0.27685922682154995 1107 80-81 0.23905891228799803 1107 82-83 0.348772990574556 1107 84-85 0.32844850265542647 1107 86-87 0.42876896748834525 1107 88-89 0.685877422894972 1107 90-91 1.1088930301171338 1107 92-93 1.1374779464341103 1107 94-95 1.072949179342146 1107 96-97 1.1544730080316405 1107 98-99 1.396890502262643 1107 100-101 1.3898413454495255 1108 1 0.1733173827873813 1108 2 -0.009579759604697813 1108 3 0.1127737285267969 1108 4 0.025543079952079495 1108 5 0.14776775877808745 1108 6 0.07555715373973015 1108 7 0.17180753835621232 1108 8 0.16064941479222483 1108 9 0.12956395888878802 1108 10-11 0.14612678114229283 1108 12-13 0.2112566955822004 1108 14-15 0.1804965907592333 1108 16-17 0.2072110721976017 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-1.2253174001533296 2316 96-97 -1.1241450938718849 2316 98-99 -1.2676509839244474 2316 100-101 -1.096602973443911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 26.0 6 72.0 7 170.0 8 457.0 9 778.0 10 1340.0 11 2256.0 12 3336.0 13 4649.0 14 6125.0 15 8356.0 16 10997.0 17 15108.0 18 13377.0 19 8157.0 20 6879.0 21 6963.0 22 7363.0 23 8077.0 24 9218.0 25 11035.0 26 14010.0 27 17244.0 28 21387.0 29 26484.0 30 33497.0 31 41771.0 32 52644.0 33 68312.0 34 91520.0 35 128454.0 36 191997.0 37 284126.0 38 209779.0 39 16730.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13928905207952 19.98297185062411 15.656218903967892 21.221520193328473 2 15.226426121281268 21.856394042010766 39.67789176516129 23.239288071546678 3 19.900626152015374 26.002017113712895 30.323463992100898 23.773892742170833 4 12.730503022215839 17.628571752582776 38.21668474829988 31.4242404769015 5 13.008611975719234 38.621811712922046 33.89584733570072 14.473728975657995 6 29.90836216939957 37.58239731096909 17.64056590008218 14.868674619549152 7 27.028353132316884 34.09686620886951 21.079736994702547 17.795043664111056 8 26.181285117243963 33.91108570875595 19.872126358572388 20.035502815427698 9 26.2635403625279 17.26490725116276 21.023170576814906 35.448381809494435 10-11 24.495789329641926 27.341006463241023 27.18836541013116 20.974838796985885 12-13 26.441950397102907 24.895460439024575 27.731315750677588 20.93127341319493 14-15 22.959155129763698 25.935729017352227 27.438210283115293 23.66690556976878 16-17 21.14587302451327 29.12682428223226 27.75653354622926 21.9707691470252 18-19 20.980984522707892 27.73389067310193 30.628814133386555 20.65631067080362 20-21 22.478082444484095 26.084612802453194 30.56931965609322 20.867985096969495 22-23 21.770990207996903 26.241540108504346 30.65833681103672 21.329132872462033 24-25 21.881131078343984 26.4194615878373 30.662593078983598 21.036814254835118 26-27 21.623366632909686 26.196662179596952 31.17084546297525 21.009125724518114 28-29 21.116919210629376 27.053113616809583 30.849513939186828 20.98045323337421 30-31 21.512453497867707 26.385914768774764 30.65666303723195 21.44496869612558 32-33 21.46356473340178 26.5459642459148 30.690397383537636 21.300073637145783 34-35 22.101427635015956 26.442207734768203 30.49376378359947 20.96260084661637 36-37 21.92632618313893 26.18616311878718 30.82663223121751 21.060878466856384 38-39 21.489673046267065 26.725635348548156 30.763348188279927 21.021343416904852 40-41 21.699587156604906 26.6362808484289 30.516048573458036 21.148083421508154 42-43 21.414433519696676 26.84777554746595 29.911203347735853 21.826587585101517 44-45 21.798845315088812 26.54251894895171 29.82217791885301 21.83645781710647 46-47 22.287748419722828 27.043435792096687 29.346206389755586 21.322609398424902 48-49 22.12204890348017 27.146402360310194 29.05997735704984 21.67157137915979 50-51 21.622888472487723 27.398986701161515 29.355916775376606 21.622208050974155 52-53 22.172311572491658 27.900002797314883 28.31929761691453 21.60838801327893 54-55 21.798097107329454 27.286387652858362 28.700156236946388 22.215359002865792 56-57 22.248216912904304 27.39890799618358 28.391002989326424 21.96187210158569 58-59 22.67589528217903 27.180780185945768 28.4124299017727 21.730894630102497 60-61 22.17123636688042 27.359382111411673 29.180772935318565 21.288608586389334 62-63 21.90733886136967 27.27899194987269 29.113782894537888 21.699886294219755 64-65 21.78731543573421 27.703994072774833 28.765564636239237 21.74312585525172 66-67 22.08051415595918 27.431643257051896 28.305529966285164 22.182312620703758 68-69 21.850296965628193 27.381924409848857 28.67676410284325 22.0910145216797 70-71 22.311286038241956 27.303576484481283 28.440220622895655 21.944916854381102 72-73 22.12875677302147 26.973012245305284 28.910094495320593 21.98813648635265 74-75 21.732073086646746 28.111164705028486 28.15531668460971 22.001445523715056 76-77 21.97071674901707 27.413290148986754 28.221711398981554 22.394281703014627 78-79 21.991139403194957 27.542923240922047 28.34086836872784 22.125068987155156 80-81 22.239204315627518 27.71762428787988 28.019710653354856 22.02346074313775 82-83 22.079936372066157 27.487138893897622 28.40927519270396 22.02364954133226 84-85 21.908907567472554 27.75100318107158 28.079241381526348 22.260847869929517 86-87 21.87383995433531 27.761636368447512 28.096523359681253 22.268000317535922 88-89 21.976007273043432 27.57254615112734 27.744279188095494 22.707167387733733 90-91 21.965926015528726 27.22531771892554 28.109978755736332 22.698777509809407 92-93 22.256297860983995 27.5207131414194 28.145074774459424 22.077914223137178 94-95 21.92011927033109 27.43353699357266 28.01968232631579 22.626661409780453 96-97 21.42427554078099 27.495787950874483 28.4356126343707 22.644323873973825 98-99 21.67737477261971 27.68063029892475 27.709889209283617 22.93210571917192 100-101 21.474664899574773 27.536997226507438 27.867457523452966 23.120880350464827 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 738.0 1 524.5 2 355.0 3 400.5 4 323.5 5 302.0 6 563.0 7 1242.0 8 2061.5 9 2459.5 10 2179.0 11 1819.5 12 1802.0 13 1943.0 14 2132.0 15 2263.0 16 2376.0 17 2533.0 18 2704.5 19 2887.0 20 3187.5 21 3552.5 22 4100.0 23 4839.0 24 5615.0 25 6704.0 26 8395.5 27 10485.0 28 12154.5 29 13984.5 30 16334.0 31 18583.0 32 21407.5 33 24396.0 34 27285.5 35 29926.5 36 33616.5 37 37328.0 38 39010.0 39 39896.0 40 40205.5 41 41131.0 42 42051.5 43 42920.5 44 44749.0 45 46272.0 46 47035.0 47 52588.0 48 65760.0 49 64423.0 50 50488.0 51 44682.5 52 42522.5 53 40779.0 54 39066.5 55 35993.5 56 32304.5 57 28874.0 58 26112.5 59 23288.0 60 19736.0 61 15773.0 62 12222.5 63 9171.0 64 6488.5 65 4705.5 66 3343.0 67 2439.0 68 1764.0 69 1205.5 70 836.5 71 557.5 72 364.0 73 271.0 74 204.0 75 152.0 76 95.0 77 40.5 78 22.5 79 11.5 80 8.5 81 7.0 82 4.0 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014818040510708303 2 0.003855714622684303 3 0.0022680674251084137 4 5.292157325252965E-4 5 0.001436442702568662 6 3.7801123751806894E-4 7 7.560224750361378E-5 8 0.0 9 0.0 10-11 7.560224750361378E-5 12-13 5.292157325252965E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002116862930101186 22-23 0.0 24-25 0.0034021011376626203 26-27 0.0017010505688313101 28-29 0.002872885405137324 30-31 0.014515631520693847 32-33 7.560224750361379E-4 34-35 0.0026460786626264824 36-37 0.003326498890159007 38-39 0.002457073043867448 40-41 0.004195924736450565 42-43 0.0020412606825975723 44-45 0.0010206303412987861 46-47 7.560224750361378E-5 48-49 3.4021011376626206E-4 50-51 1.8900561875903447E-4 52-53 0.0011340337125542068 54-55 0.003137493271399972 56-57 4.536134850216827E-4 58-59 0.0010962325888024 60-61 0.001058431465050593 62-63 0.0 64-65 1.5120449500722756E-4 66-67 3.780112375180689E-5 68-69 0.0 70-71 1.8900561875903447E-4 72-73 7.560224750361378E-5 74-75 6.048179800289102E-4 76-77 7.938235987879448E-4 78-79 1.5120449500722756E-4 80-81 0.005821373057778262 82-83 0.0017010505688313101 84-85 0.0033643000139108137 86-87 0.0020412606825975723 88-89 0.001474243826320469 90-91 1.5120449500722756E-4 92-93 7.560224750361378E-5 94-95 1.8900561875903447E-4 96-97 0.001398641578816855 98-99 0.002872885405137324 100-101 8.694258462915585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1322712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.77029557024619 #Duplication Level Percentage of deduplicated Percentage of total 1 80.03754352240277 48.63905176572861 2 12.1014961992539 14.708230017417408 3 3.503979872701548 6.388136776088002 4 1.4490988159961087 3.522486534343093 5 0.7940595159224165 2.4127615741485933 6 0.46593418191496344 1.6988974770751915 7 0.2986858999866942 1.2705861297399483 8 0.20592107889670191 1.001110786295725 9 0.16097610223746056 0.8804308781444984 >10 0.8975095833869817 9.85307368363908 >50 0.056326952128960786 2.356507494039678 >100 0.02422303452918931 2.5611921365383012 >500 0.001997760302295166 0.8400431518164222 >1k 0.001872900283401718 2.2273928932861597 >5k 3.745800566803436E-4 1.6400987016992108 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8066 0.6098077283641489 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7147 0.5403292629083277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6402 0.48400558851813547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4275 0.32319960807794895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3340 0.25251150666207006 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2765 0.2090402143474921 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2218 0.1676857849630154 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2147 0.1623180253902588 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2103 0.15899152650009982 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1834 0.1386545219216277 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1633 0.12345847017340131 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.12119040274829292 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1396 0.10554073751504484 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1391 0.10516272627752678 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1376 0.10402869256497257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.56022475036138E-5 7.56022475036138E-5 0.0 0.0 0.0 3 7.56022475036138E-5 7.56022475036138E-5 0.0 0.0 0.0 4 7.56022475036138E-5 7.56022475036138E-5 0.0 0.0 0.0 5 7.56022475036138E-5 2.2680674251084136E-4 0.0 0.0 0.0 6 7.56022475036138E-5 2.2680674251084136E-4 0.0 0.0 0.0 7 7.56022475036138E-5 2.2680674251084136E-4 0.0 0.0 0.0 8 7.56022475036138E-5 2.2680674251084136E-4 0.0 0.0 0.0 9 7.56022475036138E-5 2.2680674251084136E-4 0.0 0.0 0.0 10-11 7.56022475036138E-5 2.2680674251084136E-4 0.0 7.56022475036138E-5 0.0 12-13 7.56022475036138E-5 2.2680674251084136E-4 0.0 1.1340337125542069E-4 0.0 14-15 7.56022475036138E-5 2.2680674251084136E-4 0.0 2.2680674251084136E-4 0.0 16-17 7.56022475036138E-5 2.2680674251084136E-4 0.0 2.6460786626264824E-4 0.0 18-19 1.1340337125542069E-4 2.2680674251084136E-4 0.0 3.024089900144552E-4 0.0 20-21 1.512044950072276E-4 2.2680674251084136E-4 0.0 4.536134850216827E-4 0.0 22-23 1.512044950072276E-4 2.2680674251084136E-4 0.0 6.048179800289104E-4 0.0 24-25 1.512044950072276E-4 2.2680674251084136E-4 0.0 9.072269700433654E-4 0.0 26-27 1.512044950072276E-4 2.2680674251084136E-4 0.0 0.0010962325888024 0.0 28-29 1.512044950072276E-4 2.2680674251084136E-4 0.0 0.0023058685488602207 0.0 30-31 1.512044950072276E-4 2.2680674251084136E-4 0.0 0.004195924736450566 0.0 32-33 1.512044950072276E-4 2.2680674251084136E-4 0.0 0.007862633740375834 0.0 34-35 3.40210113766262E-4 2.2680674251084136E-4 0.0 0.011340337125542069 0.0 36-37 5.292157325252965E-4 2.2680674251084136E-4 0.0 0.015611864109496247 0.0 38-39 6.426191037807172E-4 2.6460786626264824E-4 0.0 0.018673755133392603 0.0 40-41 6.804202275325241E-4 3.024089900144552E-4 0.0 0.021357634919770895 0.0 42-43 7.182213512843311E-4 3.024089900144552E-4 0.0 0.02434392369616364 0.0 44-45 7.560224750361379E-4 3.024089900144552E-4 0.0 0.02699000235879012 0.0 46-47 9.072269700433654E-4 3.024089900144552E-4 0.0 0.03273577316906477 0.0 48-49 9.072269700433654E-4 3.024089900144552E-4 0.0 0.03787672599931051 0.0 50-51 9.072269700433654E-4 3.024089900144552E-4 0.0 0.043811502428344196 0.0 52-53 9.072269700433654E-4 3.024089900144552E-4 0.0 0.05329958449004772 0.0 54-55 9.072269700433654E-4 3.024089900144552E-4 0.0 0.07254035647971743 0.0 56-57 9.828292175469793E-4 3.024089900144552E-4 0.0 0.11071949146904239 0.0 58-59 9.828292175469793E-4 3.024089900144552E-4 0.0 0.14939004106714085 0.0 60-61 9.828292175469793E-4 3.024089900144552E-4 0.0 0.18794718729398388 0.0 62-63 9.828292175469793E-4 3.024089900144552E-4 0.0 0.23792027289387258 0.0 64-65 9.828292175469793E-4 4.536134850216827E-4 0.0 0.2988178832580335 0.0 66-67 0.001058431465050593 4.536134850216827E-4 0.0 0.3936987038750688 0.0 68-69 0.0011340337125542068 4.536134850216827E-4 0.0 0.5114114032381954 0.0 70-71 0.0011340337125542068 4.536134850216827E-4 0.0 0.6090517058891127 0.0 72-73 0.0011340337125542068 5.292157325252965E-4 0.0 0.7060493894362492 0.0 74-75 0.0011340337125542068 5.292157325252965E-4 0.0 0.8119303370650603 0.0 76-77 0.0011340337125542068 5.292157325252965E-4 0.0 0.9359180229709869 0.0 78-79 0.0011340337125542068 5.292157325252965E-4 0.0 1.0356751885520052 0.0 80-81 0.0015876471975758894 5.292157325252965E-4 0.0 1.1432571867496475 0.0 82-83 0.0015876471975758894 5.292157325252965E-4 0.0 1.2626709366816056 0.0 84-85 0.0016632494450795033 5.292157325252965E-4 0.0 1.4489170734067582 0.0 86-87 0.0017388516925831172 5.292157325252965E-4 0.0 1.687971380013185 0.0 88-89 0.0017388516925831172 5.292157325252965E-4 0.0 1.9657718384652139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7180 0.0 22.76306 1 GAGTACT 7065 0.0 22.252615 12-13 TACATGG 7420 0.0 21.701708 2 ACATGGG 7275 0.0 21.34993 3 GTACTTT 7505 0.0 21.043327 14-15 AGTACTT 7400 0.0 20.218275 12-13 CATGGGA 4930 0.0 19.364862 4 ATGGGAT 2015 0.0 19.328764 5 ACCGTGC 130 0.0075665624 18.268038 8 AGAGTAC 12440 0.0 18.116783 10-11 ACTTTTT 8900 0.0 18.038832 16-17 TACTTTT 8540 0.0 17.464062 14-15 CCTATAC 355 1.5843398E-9 17.39389 3 GTAGCAC 330 1.21090125E-8 17.272253 3 CATGGGG 2500 0.0 17.098883 4 GTATCAA 17720 0.0 16.998951 1 GCGGTAT 175 0.0021665348 16.289577 1 GGTATCA 14620 0.0 16.118734 1 TCCGTGC 180 0.00262003 15.832299 8 AGTACAT 5770 0.0 15.64141 2 >>END_MODULE