FastQCFastQC Report
Thu 26 May 2016
SRR936886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308944
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.542803970223325No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63040.4816096028554316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.391460597244802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38310.29267867838501876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.2566954736031488No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28940.22109425613318828No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23420.17892285689838525No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21260.1624210050238971No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17430.13316077693163345No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17060.13033407082350354No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16510.1261322103924996No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13910.10626887017320832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74300.024.8110621
TAGGACG1351.3395129E-524.6376884
TACATGG74800.023.8839042
ACATGGG74400.022.4166073
GAGTACT56600.021.78620112-13
AGTACTT59950.021.5595812-13
CATGGGG24850.021.4154554
GTACTTT60650.020.09634814-15
TAAGGTG4100.019.7030725
AGAGTAC110400.019.58519210-11
GTATCAA157500.019.4874231
GTATTAC2451.7518505E-719.3926141
CATGGGA52650.017.7788454
TACTTTT71800.017.73662414-15
CAACGCA180000.017.0805035
TCAACGC180300.017.0507814
TATCAAC179900.017.0352152
ATGGGAG23750.017.0068635
ACTTTTT73350.016.93943616-17
ATCAACG181400.016.921193
Produced by FastQC (version 0.11.5)