##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936886_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1308944 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.641528590986322 33.0 31.0 34.0 30.0 34.0 2 31.737794741409868 33.0 31.0 34.0 30.0 34.0 3 31.73850676575927 33.0 31.0 34.0 30.0 34.0 4 35.33494175457468 37.0 35.0 37.0 33.0 37.0 5 35.354523188157785 37.0 35.0 37.0 33.0 37.0 6 35.44232526372404 37.0 35.0 37.0 33.0 37.0 7 35.37471350951607 37.0 35.0 37.0 33.0 37.0 8 35.32984680780843 37.0 35.0 37.0 33.0 37.0 9 36.93321945018274 39.0 37.0 39.0 33.0 39.0 10-11 36.72553333068489 39.0 37.0 39.0 32.0 39.0 12-13 36.657651129460085 39.0 37.0 39.0 32.0 39.0 14-15 37.74517626422521 40.0 37.0 41.0 32.0 41.0 16-17 37.69795193682846 40.0 37.0 41.0 32.0 41.0 18-19 37.69557177388796 40.0 37.0 41.0 32.0 41.0 20-21 37.617871734772464 40.0 37.0 41.0 32.0 41.0 22-23 37.510803365155425 40.0 37.0 41.0 32.0 41.0 24-25 37.33665114779548 40.0 37.0 41.0 31.5 41.0 26-27 37.428256289039105 40.0 37.0 41.0 32.0 41.0 28-29 37.3883172236551 40.0 37.0 41.0 32.0 41.0 30-31 37.24101871432239 40.0 37.0 41.0 31.0 41.0 32-33 37.02158113716095 39.0 36.0 41.0 31.0 41.0 34-35 36.83978191580388 39.0 36.0 41.0 30.0 41.0 36-37 36.651917117921016 39.0 35.5 41.0 30.0 41.0 38-39 36.47559406666748 39.0 35.0 41.0 30.0 41.0 40-41 36.3174520835116 39.0 35.0 41.0 30.0 41.0 42-43 36.079968279773624 39.0 35.0 40.5 29.5 41.0 44-45 35.78907501008447 39.0 35.0 40.0 28.5 41.0 46-47 35.380718732046596 38.5 35.0 40.0 26.5 41.0 48-49 35.012058575462355 38.0 34.0 40.0 24.5 41.0 50-51 34.32562890391033 38.0 33.5 39.5 24.0 40.5 52-53 34.56741197484385 38.0 34.0 40.0 24.5 40.5 54-55 34.95229971641262 39.0 35.0 40.0 25.5 41.0 56-57 34.828897187351025 38.5 34.5 40.0 24.5 41.0 58-59 34.5838775379237 38.0 34.0 40.0 23.5 41.0 60-61 34.272595695461376 38.0 34.0 40.0 23.0 41.0 62-63 33.96124929714335 37.5 34.0 40.0 22.5 41.0 64-65 33.65323917600753 37.0 33.0 40.0 22.0 41.0 66-67 33.25023301226027 36.5 33.0 39.0 21.5 41.0 68-69 32.86245782860077 36.0 32.5 39.0 20.0 41.0 70-71 32.42730284870858 35.5 32.0 39.0 18.5 40.0 72-73 32.02268126061925 35.0 31.5 38.0 17.5 40.0 74-75 31.594610617413732 35.0 31.0 37.0 14.0 39.5 76-77 31.168105740199735 35.0 31.0 37.0 10.0 39.0 78-79 30.718930298011223 35.0 31.0 36.0 7.5 39.0 80-81 30.268772002469166 34.5 30.0 36.0 6.5 37.5 82-83 29.853217555525674 34.0 30.0 35.0 2.0 37.0 84-85 29.490591652507668 34.0 29.5 35.0 2.0 37.0 86-87 29.178009907222922 34.0 29.0 35.0 2.0 36.0 88-89 28.811552671466465 34.0 29.0 35.0 2.0 36.0 90-91 28.436711196200907 34.0 29.0 35.0 2.0 35.5 92-93 28.06477664437898 34.0 28.5 35.0 2.0 35.0 94-95 27.62759254788593 34.0 27.0 35.0 2.0 35.0 96-97 27.137093336307743 34.0 26.5 35.0 2.0 35.0 98-99 26.531711440672787 33.5 25.0 35.0 2.0 35.0 100-101 24.275589330024815 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6040770963335369 1101 2 0.5417086043777495 1101 3 0.6384743482607291 1101 4 0.5137528365237998 1101 5 0.6442717645370593 1101 6 0.5093074680250638 1101 7 0.5020048383637459 1101 8 0.3222031771011018 1101 9 0.37348798944587713 1101 10-11 0.51533448705419 1101 12-13 0.46378328571449856 1101 14-15 0.8743810681028421 1101 16-17 1.018871352061261 1101 18-19 0.7229503883788269 1101 20-21 0.7024638805933208 1101 22-23 0.867671911934039 1101 24-25 0.9488161506166648 1101 26-27 0.9466222560198432 1101 28-29 0.9989771311498856 1101 30-31 1.007299212237129 1101 32-33 0.9513718160349853 1101 34-35 0.8329092748538969 1101 36-37 0.8608900365070511 1101 38-39 0.8374489767987967 1101 40-41 0.9492289526592472 1101 42-43 0.9598444240589217 1101 44-45 0.9375917802313651 1101 46-47 0.7810248473811683 1101 48-49 1.0073024813764704 1101 50-51 0.959917388514242 1101 52-53 1.003059978625224 1101 54-55 0.9926437064236708 1101 56-57 1.010963587297006 1101 58-59 0.8807870450229558 1101 60-61 0.8991364374058506 1101 62-63 0.8344139472750314 1101 64-65 0.8955764123577197 1101 66-67 1.0049012115903224 1101 68-69 0.9657042432092027 1101 70-71 0.969882394590357 1101 72-73 1.0072137790534796 1101 74-75 1.0425421358284979 1101 76-77 1.0298230316420778 1101 78-79 0.9339128937083743 1101 80-81 0.9002151568920631 1101 82-83 1.0586290477265479 1101 84-85 0.9817850290654988 1101 86-87 0.9223103775726962 1101 88-89 0.9075746430546481 1101 90-91 1.1871906178955953 1101 92-93 1.2494521544984316 1101 94-95 1.3355434527004952 1101 96-97 1.5500841003498635 1101 98-99 1.7199639830768412 1101 100-101 1.7398825898660384 1102 1 0.42433218797712513 1102 2 0.42518918280923756 1102 3 0.34012021680701565 1102 4 0.22578178207307786 1102 5 0.23836477868617578 1102 6 0.26146020334750375 1102 7 0.2602931190924167 1102 8 0.2360957621016837 1102 9 0.4903450558120568 1102 10-11 0.5325004284838073 1102 12-13 0.5106619693393242 1102 14-15 0.6776570217995541 1102 16-17 0.7367551383181166 1102 18-19 0.5257588338941517 1102 20-21 0.6506264136085846 1102 22-23 0.5688302933168288 1102 24-25 0.6564507190506745 1102 26-27 0.6390628085585774 1102 28-29 0.5887896483817556 1102 30-31 0.6979206784546648 1102 32-33 0.7792488893538589 1102 34-35 0.6032498765409215 1102 36-37 0.6038444484656722 1102 38-39 0.7479231579602157 1102 40-41 0.7390340776677391 1102 42-43 0.7569009206245241 1102 44-45 0.908465421161111 1102 46-47 1.0557538324888327 1102 48-49 1.1895887162573757 1102 50-51 1.2528991716783509 1102 52-53 1.1163398082444687 1102 54-55 1.1046968265413 1102 56-57 1.1885617540256064 1102 58-59 1.079740745729815 1102 60-61 1.1317667103587041 1102 62-63 1.2305572045683633 1102 64-65 1.434555406455921 1102 66-67 1.1091665244811608 1102 68-69 1.1057239424855538 1102 70-71 1.201563665892948 1102 72-73 1.3662780434473376 1102 74-75 1.1107024311977725 1102 76-77 1.3279044499899761 1102 78-79 1.297666225203642 1102 80-81 1.2126654591740618 1102 82-83 1.024949478633232 1102 84-85 1.0476647236268164 1102 86-87 1.0131508134341694 1102 88-89 1.4491519037763716 1102 90-91 1.308132387154334 1102 92-93 1.4072691310391114 1102 94-95 1.4552804893379516 1102 96-97 1.615131670306269 1102 98-99 1.7035942040843395 1102 100-101 1.7189615987486917 1103 1 0.5883603119193985 1103 2 0.6190656610598531 1103 3 0.43872709701872026 1103 4 0.4924002421282623 1103 5 0.508766688488528 1103 6 0.3943963449723782 1103 7 0.4577402200288532 1103 8 0.5783691141022587 1103 9 0.5283667385305009 1103 10-11 0.7061288478197838 1103 12-13 0.6836852418502701 1103 14-15 0.8529790829911761 1103 16-17 0.8124741348000626 1103 18-19 0.6831207270806061 1103 20-21 0.9001462706298327 1103 22-23 0.9134864109409548 1103 24-25 0.9984798327669964 1103 26-27 0.9315557827013734 1103 28-29 1.071362189075316 1103 30-31 0.9078091878446983 1103 32-33 0.9577652796017659 1103 34-35 0.9162030956143283 1103 36-37 0.8397363220617748 1103 38-39 0.978023186679053 1103 40-41 1.0269307565399046 1103 42-43 1.0878572577425913 1103 44-45 1.2774460051790797 1103 46-47 1.26092809426099 1103 48-49 1.3700769620495592 1103 50-51 1.4018088817207115 1103 52-53 1.317721645634343 1103 54-55 1.549244479289598 1103 56-57 1.5306536901877479 1103 58-59 1.4419775325027402 1103 60-61 1.6044312538831278 1103 62-63 1.6057290047263209 1103 64-65 1.8016481118725025 1103 66-67 1.8423935724928953 1103 68-69 1.6243007961323386 1103 70-71 1.3991229497683477 1103 72-73 1.3239363560107975 1103 74-75 1.3511405985260012 1103 76-77 1.4445041915204833 1103 78-79 1.4728107168290236 1103 80-81 1.4416519920930249 1103 82-83 1.2816310944778238 1103 84-85 1.375294035297948 1103 86-87 1.5182913562201001 1103 88-89 1.6379121989649406 1103 90-91 1.6558828307577294 1103 92-93 1.792950228919043 1103 94-95 1.7356961687947567 1103 96-97 1.7543180042447482 1103 98-99 1.8682806816169837 1103 100-101 2.0634991229802075 1104 1 0.35033443172097023 1104 2 0.3412472148779848 1104 3 0.3906960347432289 1104 4 0.3206219344034409 1104 5 0.27362742645654947 1104 6 0.25814419415883805 1104 7 0.28680211314726023 1104 8 0.2646157306587398 1104 9 0.40368998945371715 1104 10-11 0.5038869705997868 1104 12-13 0.49202134231212824 1104 14-15 0.5257673926278272 1104 16-17 0.7047913282273797 1104 18-19 0.6870947009790385 1104 20-21 0.5692410925776912 1104 22-23 0.46356703500390495 1104 24-25 0.6207435199909099 1104 26-27 0.5926234163536819 1104 28-29 0.5049297221047695 1104 30-31 0.5826464303759664 1104 32-33 0.6063871238174627 1104 34-35 0.7592339566441026 1104 36-37 0.5905136336203256 1104 38-39 0.5228123063296835 1104 40-41 0.5636423764672571 1104 42-43 0.5256522084877062 1104 44-45 0.6464224694931886 1104 46-47 0.9623083882809738 1104 48-49 0.6115384838696158 1104 50-51 0.7346038827772503 1104 52-53 0.7558373317312785 1104 54-55 0.7941825207663413 1104 56-57 0.7098334378256226 1104 58-59 0.799062890871042 1104 60-61 0.8308188430601291 1104 62-63 0.7690461880913446 1104 64-65 1.0368823367243394 1104 66-67 0.8136009712914642 1104 68-69 0.7574380406760639 1104 70-71 0.6998274355352407 1104 72-73 0.7997384459475114 1104 74-75 0.8412242161188956 1104 76-77 0.8286256533693326 1104 78-79 1.0714506731587043 1104 80-81 0.8952749180044179 1104 82-83 0.8550335808685787 1104 84-85 0.6742759835576742 1104 86-87 0.7550802041890243 1104 88-89 0.7937688264893161 1104 90-91 0.8876059468463176 1104 92-93 0.8648955705839505 1104 94-95 0.8323878150852977 1104 96-97 0.90014819311385 1104 98-99 0.8741149205560141 1104 100-101 0.7641398390124472 1105 1 0.16063554417405967 1105 2 0.22091420092673886 1105 3 0.2564454262652873 1105 4 0.3090929752586504 1105 5 0.2497410726374838 1105 6 0.31481983044505313 1105 7 0.43107348757931874 1105 8 0.4248127686040277 1105 9 0.5756613356186264 1105 10-11 0.5981631174078714 1105 12-13 0.41895810098775854 1105 14-15 0.5595050318680208 1105 16-17 0.5083358575548544 1105 18-19 0.46050932378390286 1105 20-21 0.3668458492905202 1105 22-23 0.4068305203312974 1105 24-25 0.5829108150204192 1105 26-27 0.39839597225471834 1105 28-29 0.5362248837720642 1105 30-31 0.13104994822893445 1105 32-33 0.2734456759764683 1105 34-35 0.19425866898608035 1105 36-37 -0.023978420373602205 1105 38-39 0.017303271242937512 1105 40-41 0.07818496869953862 1105 42-43 0.29612172462996256 1105 44-45 0.42882843227109646 1105 46-47 0.5382836967416651 1105 48-49 0.524349621371563 1105 50-51 0.5817969914662768 1105 52-53 0.42929453881446733 1105 54-55 0.37598379902890144 1105 56-57 0.7074123747605086 1105 58-59 0.6867453271812352 1105 60-61 0.5876794395092375 1105 62-63 0.5030244652146578 1105 64-65 0.4775372290827775 1105 66-67 0.46529828517797256 1105 68-69 0.37540934034089446 1105 70-71 0.5392635976638687 1105 72-73 0.5034708722846659 1105 74-75 0.6432091574370276 1105 76-77 0.5357452429939364 1105 78-79 0.5170195832092048 1105 80-81 0.6327853652249971 1105 82-83 0.6224493882016446 1105 84-85 0.578655005661119 1105 86-87 0.5831336376688334 1105 88-89 0.7162241405300129 1105 90-91 0.3754917597960983 1105 92-93 0.2842668895830265 1105 94-95 0.1641939880924923 1105 96-97 0.2680078314166927 1105 98-99 0.43361880147666554 1105 100-101 0.36174777730929364 1106 1 0.3382042463328361 1106 2 0.3843100987848054 1106 3 0.31542187174779457 1106 4 0.12765545813278578 1106 5 0.19832907082970763 1106 6 0.22557849302009458 1106 7 0.32983959479110325 1106 8 0.2911877073660776 1106 9 0.4156532456851352 1106 10-11 0.43186308763406345 1106 12-13 0.5613785924808781 1106 14-15 0.5239208900355834 1106 16-17 0.6259257690993749 1106 18-19 0.38882919168785435 1106 20-21 0.543615047758415 1106 22-23 0.4923611049060952 1106 24-25 0.5631269538446233 1106 26-27 0.39599538531936673 1106 28-29 0.4802308598842231 1106 30-31 0.49718112139977677 1106 32-33 0.49511311565486693 1106 34-35 0.4700969319990733 1106 36-37 0.35958347532794477 1106 38-39 0.5063655506825171 1106 40-41 0.5188025437835009 1106 42-43 0.435668925596012 1106 44-45 0.3843533704993618 1106 46-47 0.2316304381710168 1106 48-49 0.24752828909474545 1106 50-51 0.33411543354911544 1106 52-53 0.4105794503767797 1106 54-55 0.41138674770289185 1106 56-57 0.31833194634990036 1106 58-59 0.36682463699840184 1106 60-61 0.4685456792203695 1106 62-63 0.4137671139917316 1106 64-65 0.2584112384514512 1106 66-67 0.3778015620755184 1106 68-69 0.46063981447979785 1106 70-71 0.3772695002311792 1106 72-73 0.5217366847239475 1106 74-75 0.30078018456202926 1106 76-77 0.4345415984907639 1106 78-79 0.47234434896292754 1106 80-81 0.4454292669928037 1106 82-83 0.3253812773671818 1106 84-85 0.6448619774756494 1106 86-87 0.5067089730985934 1106 88-89 0.6368191302278028 1106 90-91 0.7583386690737832 1106 92-93 0.6967894423484857 1106 94-95 0.6476855417037228 1106 96-97 0.6755317068032483 1106 98-99 0.6044451015894765 1106 100-101 0.6519797757245236 1107 1 0.3000670113285011 1107 2 0.17646724190552732 1107 3 0.1216994322193905 1107 4 0.23110985766045644 1107 5 0.314081898372649 1107 6 0.2880969435450069 1107 7 0.24423492496324428 1107 8 0.2618594448520071 1107 9 0.3244107873371931 1107 10-11 0.513602372491718 1107 12-13 0.37400543983967793 1107 14-15 0.4474820950393692 1107 16-17 0.5192886540234483 1107 18-19 0.44887642503164926 1107 20-21 0.48706880151771514 1107 22-23 0.46385907996098297 1107 24-25 0.42111384013404063 1107 26-27 0.36948025127630757 1107 28-29 0.5297618706039771 1107 30-31 0.4579420441105242 1107 32-33 0.4171913597184087 1107 34-35 0.39541612608014276 1107 36-37 0.24778251959074993 1107 38-39 0.07927452963047443 1107 40-41 0.21227244175209847 1107 42-43 0.2816739256882599 1107 44-45 0.290770001802521 1107 46-47 0.05220946176924457 1107 48-49 0.14987369675909434 1107 50-51 0.013475292587109777 1107 52-53 -0.046124663167752544 1107 54-55 0.05651800012452668 1107 56-57 0.3146591387335178 1107 58-59 0.13542968506202158 1107 60-61 0.15937715212577785 1107 62-63 0.31745249102821305 1107 64-65 0.20805399016273185 1107 66-67 0.25139182665604665 1107 68-69 0.2661476421149942 1107 70-71 0.21590120930329704 1107 72-73 0.15626407452818825 1107 74-75 0.28766935687148276 1107 76-77 0.3958174061624895 1107 78-79 0.3186111158774452 1107 80-81 0.4142150148848316 1107 82-83 0.42709671492137957 1107 84-85 0.39960145606381303 1107 86-87 0.3639070994477862 1107 88-89 0.5025690748762273 1107 90-91 0.32516185769349804 1107 92-93 0.13240518249129352 1107 94-95 0.27226521568294615 1107 96-97 0.1895373849522919 1107 98-99 0.15049472888887294 1107 100-101 0.30392100157739677 1108 1 0.2185272022405158 1108 2 0.18048479580063415 1108 3 0.19864743193338086 1108 4 0.1512012022673659 1108 5 0.2172874505516873 1108 6 0.14279690064343242 1108 7 0.3054031885219288 1108 8 0.21876347044995015 1108 9 0.16001730837684391 1108 10-11 0.1540134232228283 1108 12-13 0.2997477264937203 1108 14-15 0.5158129108843497 1108 16-17 0.5365073805616518 1108 18-19 0.5540299769246744 1108 20-21 0.5185299577152023 1108 22-23 0.5516162659750705 1108 24-25 0.5120774721100148 1108 26-27 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15 8637.0 16 10850.0 17 14670.0 18 12675.0 19 8290.0 20 7914.0 21 8326.0 22 8899.0 23 10120.0 24 11296.0 25 12987.0 26 15187.0 27 17976.0 28 20960.0 29 25414.0 30 31296.0 31 38809.0 32 48162.0 33 62845.0 34 84574.0 35 118516.0 36 174119.0 37 252437.0 38 226786.0 39 39025.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23684821952651 19.392991219917825 14.879824153785954 21.49033640676971 2 15.019003996456764 21.253140264244593 40.44805561563701 23.279800123661637 3 19.734899544258298 26.157006226330314 29.93383950922946 24.174254720181928 4 12.290400317337301 17.185205906203592 38.783173480017766 31.74122029644134 5 13.005817464625075 38.48356050239655 33.932942444558265 14.57767958842011 6 29.73037847920314 37.35607317315557 17.981928401611462 14.931619946029828 7 27.017853661892694 33.99565029603214 21.22617313962244 17.760322902452728 8 25.86921937865559 33.81899993807808 20.204434068063655 20.107346615202673 9 26.291455227360643 17.477666046427714 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34-35 22.123914427904033 26.809261358274988 29.82886421261378 21.2379600012072 36-37 21.945436285477548 26.499199502472926 30.210864533509156 21.344499678540362 38-39 21.62052100153991 27.093554658326486 30.108542305827772 21.177382034305833 40-41 21.74890838869385 27.008622105749723 29.9034645049643 21.339005000592127 42-43 21.461833134181486 27.146179493369928 29.27929442181278 22.11269295063581 44-45 21.79544625445739 26.907437224129005 29.255475400568027 22.041641120845583 46-47 22.26424175748616 27.34616075146209 28.85706864082083 21.532528850230918 48-49 22.093606013447594 27.345708298285704 28.65253607327495 21.908149614991753 50-51 21.539023973760543 27.66080014913645 29.00837631893502 21.79179955816799 52-53 22.042351686392916 28.12731711658919 28.014510187577336 21.815821009440562 54-55 21.742987103628245 27.47982685649649 28.399522789549504 22.37766325032576 56-57 22.16147607408797 27.723308321237383 27.991450623875973 22.123764980798676 58-59 22.637550121023935 27.372153186963644 28.092222684041957 21.898074007970465 60-61 22.2099240651416 27.521276087683272 28.8353789579254 21.433420889249724 62-63 21.86526870271528 27.617199632782313 28.772453019479116 21.745078645023288 64-65 21.679917910037226 28.002923270640157 28.443858839493647 21.873299979828975 66-67 21.919579256064754 27.78354885406037 27.89056103648615 22.406310853388725 68-69 21.713821314654876 27.638846647032484 28.392016824061095 22.255315214251546 70-71 22.256034311820823 27.57408381654057 28.101132151018167 22.068749720620445 72-73 22.006948018233103 27.270341177266154 28.626133121828722 22.09657768267202 74-75 21.588311547893902 28.466265075431117 27.86563567725635 22.079787699418628 76-77 21.869496891991698 27.670042574880476 27.967145931773974 22.493314601353852 78-79 21.89806487655998 27.80336110241775 28.03275124104918 22.265822779973092 80-81 22.12907759305515 28.010219034441857 27.71848134388098 22.142222028622008 82-83 22.02018411162245 27.77623239086809 28.172968024246607 22.030615473262856 84-85 21.863623998606226 28.017362586308604 27.875043173405796 22.243970241679374 86-87 21.83524958086065 28.10984583924917 27.858132984470828 22.196771595419346 88-89 21.948900123176422 27.858846848609147 27.46031915836576 22.73193386984867 90-91 22.001409007100815 27.54295599603583 27.77088811001259 22.68474688685077 92-93 22.255732583496012 27.819254969525442 27.89238932696904 22.032623120009507 94-95 21.93953829395486 27.727197528133075 27.829211326358948 22.504052851553112 96-97 21.429832438525764 27.808020787558036 28.222128814077458 22.540017959838742 98-99 21.63082822888954 28.07085466103525 27.50487194588519 22.793445164190025 100-101 21.74648083282752 27.666953082357757 27.754980040088007 22.831586044726716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 517.0 1 363.0 2 252.0 3 302.5 4 281.5 5 305.5 6 527.0 7 1031.5 8 1716.0 9 2105.5 10 1895.0 11 1670.0 12 1751.5 13 1904.0 14 2071.0 15 2148.0 16 2163.0 17 2244.5 18 2428.5 19 2597.5 20 2800.0 21 3190.0 22 3800.0 23 4618.5 24 5483.5 25 6607.5 26 8281.0 27 10257.0 28 11867.0 29 13755.5 30 16359.5 31 18895.5 32 21516.5 33 24284.0 34 27294.0 35 30073.0 36 33479.5 37 36996.5 38 38858.5 39 39822.0 40 40275.5 41 41064.0 42 41677.0 43 42449.0 44 44248.0 45 45948.0 46 46832.0 47 51718.0 48 63927.0 49 63475.0 50 50887.0 51 44750.5 52 42211.5 53 40407.0 54 38675.5 55 35565.0 56 31937.0 57 28493.5 58 25740.0 59 23041.0 60 19510.0 61 15876.5 62 12274.5 63 9106.5 64 6510.0 65 4665.5 66 3329.5 67 2449.0 68 1811.0 69 1236.5 70 823.0 71 550.5 72 364.5 73 273.5 74 193.5 75 133.0 76 93.5 77 44.5 78 27.5 79 20.5 80 11.5 81 10.0 82 10.5 83 8.5 84 5.0 85 4.5 86 6.0 87 6.0 88 4.0 89 0.5 90 0.5 91 0.5 92 1.5 93 2.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04033786013763767 2 0.040872642374310895 3 0.023530418413621974 4 0.042553386546712466 5 0.06218753437885807 6 0.06218753437885807 7 0.061805547066948616 8 0.06455585571269665 9 0.06463225317507854 10-11 0.07265398672517694 12-13 0.07051485777848403 14-15 0.0663129973474801 16-17 0.05462418560305101 18-19 0.0477866127198719 20-21 0.055961141194734075 22-23 0.0309791709958562 24-25 0.016616448068060972 26-27 0.012223593981102324 28-29 0.016501851874488136 30-31 0.012643780024202715 32-33 0.006417386840078721 34-35 0.009893471378454692 36-37 0.005386021097923212 38-39 0.007601547506998007 40-41 0.007563348775807063 42-43 0.004583847742913372 44-45 0.0016425454412106247 46-47 0.0022155264090747964 48-49 8.403720862007848E-4 50-51 0.005768008409832659 52-53 0.005386021097923212 54-55 0.006684777958415333 56-57 0.023721412069576696 58-59 0.008556515786771626 60-61 0.019595949100954663 62-63 0.014018934347076727 64-65 0.010390054883936975 66-67 0.016387255680915303 68-69 0.026127932134606218 70-71 0.01837358970284443 72-73 0.017227627767116088 74-75 0.017838807466171204 76-77 0.02356861714481292 78-79 0.03625059590020658 80-81 0.0309791709958562 82-83 0.029795010328936913 84-85 0.020474519918346392 86-87 0.02368321333838575 88-89 0.01925216052023616 90-91 0.016883839186397586 92-93 0.030062401447273526 94-95 0.022728245058612134 96-97 0.035830409857106185 98-99 0.052599652849930935 100-101 0.06279871407791318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1308944.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.58651702752116 #Duplication Level Percentage of deduplicated Percentage of total 1 81.09408199951481 50.7539614389383 2 11.516793344954893 14.415919655729233 3 3.33951383717182 6.270256189013898 4 1.3580081927241794 3.399720115097804 5 0.7150058815492119 2.2374863890178758 6 0.4327208332171001 1.6249493877783108 7 0.2768167834248126 1.2127498830524468 8 0.20731017766513893 1.0379857571534123 9 0.14962646753431283 0.8428139512293702 >10 0.8307751516255648 9.385800288486877 >50 0.05327386031628584 2.251631435223664 >100 0.022278774812822724 2.364564849589846 >500 0.00171373344665844 0.7267401279454933 >1k 0.00171373344665844 2.0555728835025855 >5k 3.6722859571252286E-4 1.4198476482409683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7105 0.542803970223325 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6304 0.4816096028554316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.391460597244802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3831 0.29267867838501876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.2566954736031488 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2894 0.22109425613318828 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2342 0.17892285689838525 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2126 0.1624210050238971 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1743 0.13316077693163345 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1706 0.13033407082350354 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1651 0.1261322103924996 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1391 0.10626887017320832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 1.5279492476377905E-4 0.0 0.0 0.0 14-15 7.639746238188952E-5 1.5279492476377905E-4 0.0 0.0 0.0 16-17 7.639746238188952E-5 1.5279492476377905E-4 0.0 0.0 0.0 18-19 7.639746238188952E-5 1.5279492476377905E-4 0.0 3.819873119094476E-5 0.0 20-21 7.639746238188952E-5 1.5279492476377905E-4 0.0 1.1459619357283429E-4 0.0 22-23 7.639746238188952E-5 1.5279492476377905E-4 0.0 4.965835054822819E-4 0.0 24-25 1.5279492476377905E-4 1.5279492476377905E-4 0.0 7.257758926279504E-4 0.0 26-27 1.5279492476377905E-4 1.5279492476377905E-4 0.0 0.0012987568604921219 0.0 28-29 2.6739111833661336E-4 1.5279492476377905E-4 0.0 0.002673911183366133 0.0 30-31 3.819873119094476E-4 1.5279492476377905E-4 0.0 0.00466024520529526 0.0 32-33 3.819873119094476E-4 1.5279492476377905E-4 0.0 0.007754342431761787 0.0 34-35 4.965835054822819E-4 1.5279492476377905E-4 0.0 0.01123042697013776 0.0 36-37 5.347822366732266E-4 1.5279492476377905E-4 0.0 0.01420992800303145 0.0 38-39 5.347822366732266E-4 1.5279492476377905E-4 0.0 0.017762410003789315 0.0 40-41 5.347822366732266E-4 1.5279492476377905E-4 0.0 0.020741911036683006 0.0 42-43 5.347822366732266E-4 1.5279492476377905E-4 0.0 0.023454020951240086 0.0 44-45 5.347822366732266E-4 1.5279492476377905E-4 0.0 0.026853708027234167 0.0 46-47 6.493784302460609E-4 1.5279492476377905E-4 0.0 0.032125132931584546 0.0 48-49 6.875771614370057E-4 1.5279492476377905E-4 0.0 0.037129166717598305 0.0 50-51 6.875771614370057E-4 1.5279492476377905E-4 0.0 0.04201860431003924 0.0 52-53 6.875771614370057E-4 1.5279492476377905E-4 0.0 0.0506897162903837 0.0 54-55 6.875771614370057E-4 2.2919238714566857E-4 0.0 0.06745895928320844 0.0 56-57 8.403720862007847E-4 2.2919238714566857E-4 0.0 0.10542849808700754 0.0 58-59 8.403720862007847E-4 3.055898495275581E-4 0.0 0.148669461795157 0.0 60-61 8.403720862007847E-4 3.055898495275581E-4 0.0 0.18656260313657422 0.0 62-63 8.403720862007847E-4 3.055898495275581E-4 0.0 0.2388566661369776 0.0 64-65 8.403720862007847E-4 3.055898495275581E-4 0.0 0.2999746360424892 0.0 66-67 9.167695485826743E-4 3.055898495275581E-4 0.0 0.3950130792455598 0.0 68-69 9.931670109645638E-4 3.055898495275581E-4 0.0 0.51308535735677 0.0 70-71 0.0010313657421555085 3.055898495275581E-4 0.0 0.6095371536139056 0.0 72-73 0.0011077632045373982 3.055898495275581E-4 0.0 0.7090830470975076 0.0 74-75 0.001145961935728343 3.819873119094476E-4 0.0 0.8189043992714737 0.0 76-77 0.001145961935728343 3.819873119094476E-4 0.0 0.9441962375777726 0.0 78-79 0.001145961935728343 3.819873119094476E-4 0.0 1.0441623171044752 0.0 80-81 0.0014897505164468458 3.819873119094476E-4 0.0 1.145885538265961 0.0 82-83 0.0015279492476377905 3.819873119094476E-4 0.0 1.2626590595166791 0.0 84-85 0.00160434671001968 3.819873119094476E-4 0.0 1.447349924824897 0.0 86-87 0.0016425454412106247 3.819873119094476E-4 0.0 1.6999581341906147 0.0 88-89 0.0016807441724015694 3.819873119094476E-4 0.0 1.9831635272402792 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7430 0.0 24.811062 1 TAGGACG 135 1.3395129E-5 24.637688 4 TACATGG 7480 0.0 23.883904 2 ACATGGG 7440 0.0 22.416607 3 GAGTACT 5660 0.0 21.786201 12-13 AGTACTT 5995 0.0 21.55958 12-13 CATGGGG 2485 0.0 21.415455 4 GTACTTT 6065 0.0 20.096348 14-15 TAAGGTG 410 0.0 19.703072 5 AGAGTAC 11040 0.0 19.585192 10-11 GTATCAA 15750 0.0 19.487423 1 GTATTAC 245 1.7518505E-7 19.392614 1 CATGGGA 5265 0.0 17.778845 4 TACTTTT 7180 0.0 17.736624 14-15 CAACGCA 18000 0.0 17.080503 5 TCAACGC 18030 0.0 17.050781 4 TATCAAC 17990 0.0 17.035215 2 ATGGGAG 2375 0.0 17.006863 5 ACTTTTT 7335 0.0 16.939436 16-17 ATCAACG 18140 0.0 16.92119 3 >>END_MODULE